Mcl-1 Antibody

Images

 
Western Blot: MCL1 Antibody [NB100-56146] - analysis of Mcl-1 in A) Ramos and B) ThP-1 lysate using Mcl-1 antibody at 1:2000.
Immunohistochemistry-Paraffin: MCL1 Antibody [NB100-56146] - Formalin-fixed paraffin-embedded tissue sections of human diffuse, aggressive Non-Hodgkin's lymphoma (NHL) of B cell lineage stained for Mcl-1 expression ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Mcl-1 Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 121-139 (SPEEELDGYEPEPLGKRPA) of human Mcl-1 was used as immunogen, GenBank no. NP_068779.1. The immunogen sequence is 100% conserved in Mcl-1 isoform 1 (GenBank no. NP_068779.1) and isoform 2 (GenBank no. NP_877495.1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MCL1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
Application Notes
Immunoprecipitation, Western Blot, Immunohistochemistry-Paraffin IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents)
Control
Human Lymph Node Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 1 Review rated 5
using
NB100-56146 in the following applications:

Publications
Read Publications using
NB100-56146 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for Mcl-1 Antibody

  • BCL2L3
  • bcl2-L-3
  • BCL2L3MGC104264
  • Bcl-2-like protein 3
  • Bcl-2-related protein EAT/mcl1
  • EAT
  • induced myeloid leukemia cell differentiation protein Mcl-1
  • Mcl1
  • Mcl-1
  • mcl1/EAT
  • MCL1-ES
  • MCL1L
  • MCL1S
  • MGC1839
  • myeloid cell leukemia ES
  • myeloid cell leukemia sequence 1 (BCL2-related)
  • TM

Background

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. This antibody recognizes Mcl-1, isoforms 1 and 2. Human Mcl-1 isoform 1 is a 350 amino acid protein, GenBank no. NP_068779.1. Human Mcl-1 isoform 2 is 271 amino acid protein, GenBank no. NP_877495.1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for Mcl-1 Antibody (NB100-56146)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IF/IHC, WB.


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IF/IHC
(1)
WB
(3)
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Human
(3)
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Showing Publications 1 - 5 of 5.
Publications using NB100-56146 Applications Species
Srivastava S, Gajwani P, Jousma J et al. Nuclear translocation of mitochondrial dehydrogenases as an adaptive cardioprotective mechanism Nature communications 2023-07-19 [PMID: 37468519] (WB, Human)

Details:
Dilutions: 1:1000
WB Human
Saleh T, El-Sadoni M, Alhesa A Et al. Expression of Senescence and Apoptosis Biomarkers in Synchronous Bilateral Breast Cancer: A Case Report Current oncology (Toronto, Ont.) 2021-09-30 [PMID: 34677245] (IF/IHC, Human) IF/IHC Human
Ohta K, Iwai K, Kasahara Y et al. Immunoblot analysis of cellular expression of Bcl-2 family proteins, Bcl-2, Bax, Bcl-X and Mcl-1, in human peripheral blood and lymphoid tissues. Int Immunol. 1995-11-01 [PMID: 8580080] (WB, Human)

Details:
WB: Bcl-2 (IMG-5685), Mcl-1 (IMG-5733), Bax (IMG-5682), Bcl-X (IMG-80459). 1. WB: Human PBMC, neutrophils, tonsil, spleen, thymus, Fig 1A.
WB Human
Reynolds JE, Yang T, Qian L et al. Mcl-1, a member of the Bcl-2 family, delays apoptosis induced by c-Myc overexpression in Chinese hamster ovary cells. Cancer Res. 1994-12-15 [PMID: 7987827] (WB)

Details:
Mcl-1 (IMG-5733). WB: Untransfected Chinese hamster ovary 5AHSmyc cells, Bcl-2 transfected 5AHSmyc cells, and human myeloblastic ML-1 cells treated with TPA, Fig 2C.
WB
Krajewski S, Bodrug S, Krajewska M et al. Immunohistochemical analysis of Mcl-1 protein in human tissues. Differential regulation of Mcl-1 and Bcl-2 protein production suggests a unique role for Mcl-1 in control of programmed cell death in vivo. Am J Pathol. 1995-06-01 [PMID: 7778670]

Review for Mcl-1 Antibody (NB100-56146) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56146:
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WB
(1)
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Human
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Images Ratings Applications Species Date Details
Western Blot Mcl-1 NB100-56146
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5
reviewed by:
Verified Customer
WB Human 06/15/2016
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from UW228 cells
SpeciesHuman
LotAR03061206B-02

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Verified Customer
06/15/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol MCL1
Entrez