Mcl-1 Antibody

Images

 
Western Blot: MCL1 Antibody [NB100-56146] - analysis of Mcl-1 in A) Ramos and B) ThP-1 lysate using Mcl-1 antibody at 1:2000.
Immunohistochemistry-Paraffin: MCL1 Antibody [NB100-56146] - Formalin-fixed paraffin-embedded tissue sections of human diffuse, aggressive Non-Hodgkin's lymphoma (NHL) of B cell lineage stained for Mcl-1 expression ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Mcl-1 Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 121-139 (SPEEELDGYEPEPLGKRPA) of human Mcl-1 was used as immunogen, GenBank no. NP_068779.1. The immunogen sequence is 100% conserved in Mcl-1 isoform 1 (GenBank no. NP_068779.1) and isoform 2 (GenBank no. NP_877495.1).
Specificity
Mcl-1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MCL1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
Application Notes
Immunoprecipitation, Western Blot, Immunohistochemistry-Paraffin IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents)
Positive Control
Lymph Node Lysate (NB820-59242)
Reviewed Applications
Read 1 Review rated 5
using
NB100-56146 in the following applications:

Publications
Read Publications using
NB100-56146 in the following applications:

  • WB
    2 publications

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for Mcl-1 Antibody

  • BCL2L3
  • bcl2-L-3
  • BCL2L3MGC104264
  • Bcl-2-like protein 3
  • Bcl-2-related protein EAT/mcl1
  • EAT
  • induced myeloid leukemia cell differentiation protein Mcl-1
  • Mcl1
  • Mcl-1
  • mcl1/EAT
  • MCL1-ES
  • MCL1L
  • MCL1S
  • MGC1839
  • myeloid cell leukemia ES
  • myeloid cell leukemia sequence 1 (BCL2-related)
  • TM

Background

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. This antibody recognizes Mcl-1, isoforms 1 and 2. Human Mcl-1 isoform 1 is a 350 amino acid protein, GenBank no. NP_068779.1. Human Mcl-1 isoform 2 is 271 amino acid protein, GenBank no. NP_877495.1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC, IHC-P, IP
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Publications for Mcl-1 Antibody (NB100-56146)(3)

Review for Mcl-1 Antibody (NB100-56146) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-56146:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot Mcl-1 NB100-56146
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reviewed by:
Anonymous
WB Human 06/15/2016
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from UW228 cells
SpeciesHuman
LotAR03061206B-02

Blocking

Blocking Details5% Milk in PBST for 1 hour at room temperature

Primary Anitbody

Dilution Ratio1:2000 in 5% milk PBST overnight at 4 degrees C

Secondary Antibody

Secondary DescriptionSouthern Biotech: donkey anti rabbit HRP
Secondary Manufacturer Cat#6440-05
Secondary Concentration1:5000

Details

Detection Notes5 second exposure

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Mcl-1 Antibody (NB100-56146) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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2
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1
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Anonymous
06/15/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol MCL1
Entrez