Mcl-1 Antibody


Western Blot: MCL1 Antibody [NB100-56146] - analysis of Mcl-1 in A) Ramos and B) ThP-1 lysate using Mcl-1 antibody at 1:2000.
Immunohistochemistry-Paraffin: MCL1 Antibody [NB100-56146] - Formalin-fixed paraffin-embedded tissue sections of human diffuse, aggressive Non-Hodgkin's lymphoma (NHL) of B cell lineage stained for Mcl-1 expression more

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Mcl-1 Antibody Summary

A synthetic peptide corresponding to amino acids 121-139 (SPEEELDGYEPEPLGKRPA) of human Mcl-1 was used as immunogen, GenBank no. NP_068779.1. The immunogen sequence is 100% conserved in Mcl-1 isoform 1 (GenBank no. NP_068779.1) and isoform 2 (GenBank no. NP_877495.1).
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
This product is unpurified. The exact concentration of antibody is not quantifiable.


Application Notes
Immunoprecipitation, Western Blot, Immunohistochemistry-Paraffin IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents)
Reviewed Applications
Read 1 Review rated 5
NB100-56146 in the following application:

Read Publications using
NB100-56146 in the following applications:

  • WB
    2 publications

Reactivity Notes

Cross reacts with Human.

Alternate Names for Mcl-1 Antibody

  • BCL2L3
  • bcl2-L-3
  • BCL2L3MGC104264
  • Bcl-2-like protein 3
  • Bcl-2-related protein EAT/mcl1
  • EAT
  • induced myeloid leukemia cell differentiation protein Mcl-1
  • Mcl1
  • Mcl-1
  • mcl1/EAT
  • MCL1-ES
  • MCL1L
  • MCL1S
  • MGC1839
  • myeloid cell leukemia ES
  • myeloid cell leukemia sequence 1 (BCL2-related)
  • TM


The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. This antibody recognizes Mcl-1, isoforms 1 and 2. Human Mcl-1 isoform 1 is a 350 amino acid protein, GenBank no. NP_068779.1. Human Mcl-1 isoform 2 is 271 amino acid protein, GenBank no. NP_877495.1.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Mcl-1 Antibody (NB100-56146)(3)

Reviews for Mcl-1 Antibody (NB100-56146) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-56146:
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Western Blot Mcl-1 NB100-56146
reviewed by:
WB Human 06/15/2016


ApplicationWestern Blot
Sample TestedWhole cell lysate from UW228 cells


Blocking Details5% Milk in PBST for 1 hour at room temperature

Primary Anitbody

Dilution Ratio1:2000 in 5% milk PBST overnight at 4 degrees C

Secondary Antibody

Secondary DescriptionSouthern Biotech: donkey anti rabbit HRP
Secondary Manufacturer Cat#6440-05
Secondary Concentration1:5000


Detection Notes5 second exposure

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Mcl-1 Antibody (NB100-56146) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol MCL1

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