BID Antibody [Unconjugated]

Images

 
Western blot shows Jurkat human acute T cell leukemia cell line treated with apoptosis inducer anti-Fas for the indicated times. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse BID Antigen Affinity-purified ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for BID at approximately 29 kDa (as indicated) using 10 µg/mL of Goat ...read more
Western blot shows lysates of SVEC4‑10 mouse vascular endothelial cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse BID Antigen ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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BID Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse BID
Met1-Asp195
Accession # AAC71064
Specificity
Detects human and mouse BID, a fragment generated in cells treated with anti-Fas and the carboxyl‑terminal portion of recombinant BID generated by cleavage with Caspase 8 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BID
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunoprecipitation 4 ug/10^6 cells
Reviewed Applications
Read 1 Review rated 4
using
AF860 in the following applications:

Publications
Read Publications using
AF860 in the following applications:

  • WB
    16 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BID Antibody [Unconjugated]

  • apoptic death agonist
  • BH3 interacting domain death agonist
  • BH3-interacting domain death agonist
  • BID isoform ES(1b)
  • BID isoform L(2)
  • BID isoform Si6
  • BID
  • desmocollin type 4
  • FP497
  • Human BID coding sequence
  • MGC15319
  • MGC42355
  • p22 BID

Background

BID is a 195 amino acid member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1). BID is a pro-apoptotic member that causes cytochrome c to be released from the mitochondria intermembrane space into the cytosol. In healthy cells BID is cytosolic. In response to Fas ligand or TNF, BID is cleaved by caspase-8 and it then relocates to the mitochondria outer membrane (2, 3). Cleavage of BID by caspase-8 generates a new N-terminal that contains a terminal glycine. It appears that the glycine is myristoylated and myristoylation serves to target BID to the mitochondria (4). BID may then interact with another pro-apoptotic Bcl-2 family member Bak (5). Interaction of BID with Bak causes altered mitochondrial membrane permeability. A 9‑13 amino acid stretch called the BH3 region (Bcl-2 homology region) appears to mediate the BID interaction with other Bcl-2 family members. BID is neutralized by binding to the anti-apoptotic member Bcl-xL.

  1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
  2. Luo, X., et al. (1998) Cell 94:481.
  3. Li, H. et al. (1998) Cell 94:491.
  4. Zha, J. et al. (2000) Science 290:1761.
  5. Wei, M.C. et al. (2000) Genes Dev. 14:2060.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BID Antibody (AF860)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(16)
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Filter By Species
Human
(7)
Mouse
(9)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using AF860 Applications Species
S Naghdi, WS Slovinsky, M Madesh, E Rubin, G Hajnóczky Mitochondrial fusion and Bid-mediated mitochondrial apoptosis are perturbed by alcohol with distinct dependence on its metabolism Cell Death Dis, 2018;9(10):1028. 2018 [PMID: 30301883] (WB,, Human) WB Human
NM de Vasconc, N Van Opdenb, H Van Gorp, E Parthoens, M Lamkanfi Single-cell analysis of pyroptosis dynamics reveals conserved GSDMD-mediated subcellular events that precede plasma membrane rupture Cell Death Differ., 2018;0(0):. 2018 [PMID: 29666477] (WB,, Mouse) WB Mouse
MA Miles, CJ Hawkins Executioner caspases and CAD are essential for mutagenesis induced by TRAIL or vincristine Cell Death Dis, 2017;8(10):e3062. 2017 [PMID: 28981092] (WB,, Human) WB Human
B Lacoste, VA Raymond, S Cassim, P Lapierre, M Bilodeau Highly tumorigenic hepatocellular carcinoma cell line with cancer stem cell-like properties PLoS ONE, 2017;12(2):e0171215. 2017 [PMID: 28152020] (WB,, Mouse) WB Mouse
Rizvi S, Mertens J, Bronk S, Hirsova P, Dai H, Roberts L, Kaufmann S, Gores G Platelet-derived growth factor primes cancer-associated fibroblasts for apoptosis. J Biol Chem, 2014;289(33):22835-49. 2014 [PMID: 24973208] (WB,, Human) WB Human
Chu H, Liao Y, Novak J, Hu Z, Merkin J, Shymkiv Y, Braeckman B, Dorovkov M, Nguyen A, Clifford P, Nagele R, Harrison D, Ellis R, Ryazanov A Germline quality control: eEF2K stands guard to eliminate defective oocytes. Dev Cell, 2014;28(5):561-72. 2014 [PMID: 24582807] (WB,, Mouse) WB Mouse
Kurita S, Mott JL, Almada LL GLI3-dependent repression of DR4 mediates hedgehog antagonism of TRAIL-induced apoptosis. Oncogene, 2010;29(34):4848-58. 2010 [PMID: 20562908] (WB,, Human) WB Human
Park SJ, Sohn HY, Yoon J, Park SI Down-regulation of FoxO-dependent c-FLIP expression mediates TRAIL-induced apoptosis in activated hepatic stellate cells. Cell. Signal., 2009;21(10):1495-503. 2009 [PMID: 19470406] (WB,, Human) WB Human
Park SJ, Kim MJ, Kim HB, Sohn HY, Bae JH, Kang CD, Kim SH Trichostatin A sensitizes human ovarian cancer cells to TRAIL-induced apoptosis by down-regulation of c-FLIPL via inhibition of EGFR pathway. Biochem. Pharmacol., 2009;77(8):1328-36. 2009 [PMID: 19426671] (WB,, Human) WB Human
Souktani R, Pons S, Guegan C, Bouhidel O, Bruneval P, Zini R, Mandet C, Onteniente B, Berdeaux A, Ghaleh B Cardioprotection against myocardial infarction with PTD-BIR3/RING, a XIAP mimicking protein. J. Mol. Cell. Cardiol., 2009;46(5):713-8. 2009 [PMID: 19233193] (WB,, Mouse) WB Mouse
Show All 16 Publications.

Review for BID Antibody (AF860) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF860:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/09/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21048194
SpeciesHuman

Blocking

Blocking DetailsSee PMID 21048194

Details

Detection NotesSee PMID 21048194

Comments

CommentsPublished: See PMID 21048194Independent review from antibodyresource.comSample loading buffer was added and equal amounts of protein (50 ?g/sample) were separated on 4%–12% or 12% polyacrylamide Criterion XT-gels in MOPS buffer (Bio-Rad) at 150 V. Subsequently, proteins were transferred onto a PVDF-membrane. Membranes were blocked for 30 min in 5% skimmed milk powder containing Tris-buffered saline and 0.1% Tween-20 (TBS-T) and probed for 1 h with primary antibody in 5% skimmed milk powder in TBS-T. Following three 10 min washes in TBS-T, membranes were incubated with species-specific HRP-conjugated antibody for 1 h in 5% skimmed milk powder containing TBS-T. Again following a threefold wash in TBS-T or TBS (last wash step), bands were visualized on ECL Hyperfilms (Amersham Biosciences, Buckinghamshire, UK) using an enhanced chemiluminiscence kit (PerkinElmer Life Sciences, Boston, MA).

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Blogs on BID.

Apoptosis and Necroptosis Part I: Important factors to identify both types of programmed cell death
Different types of cell death have classically been identified by discrete morphological changes. The hallmarks of apoptosis include cell shrinkage, nuclear fragmentation and membrane blebbing whereas necroptosis is characterized by cell swelling...  Read full blog post.

The use of apoptosis antibodies and controls in cell death research
Apoptosis is a method of programmed cell death that is notably characterized by a morphological change in cellular nuclei and membrane appearance.  Not to be confused with necrosis, apoptosis is a pathway that is induced by a variety of factors tha...  Read full blog post.

The role of p53 in UV radiation DNA damage and subsequent tumorogenesis
p53, the protein product of the tp53 gene, is one of the most widely studied tumor suppressor proteins in cancer research.  p53 is unique in that it demonstrates both tumor suppressive and tumor progressive properties depending on whether it is fu...  Read full blog post.

active/cleaved Caspase 2 - Inducing apoptosis in response to cellular stress
Caspase-2 is a highly conserved member of the caspase family involved in the initiation and execution of apoptosis. While its function is still poorly understood, caspase-2 is thought to be important for apoptosis in response to DNA damage, bacteri...  Read full blog post.

Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival
B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co...  Read full blog post.

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Recent Reviews

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5
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4
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1
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Anonymous
01/09/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol BID
Uniprot