BID Antibody [Unconjugated]

Images

 
Western blot shows Jurkat human acute T cell leukemia cell line treated with apoptosis inducer anti-Fas for the indicated times. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse BID Antigen Affinity-purified ...read more
Western blot shows lysates of SVEC4-10 mouse vascular endothelial cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse BID Antigen Affinity-purified ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for BID at approximately 29 kDa (as indicated) using 10 µg/mL of Goat ...read more
Survivin is predominantly expressed in obese hASCs and is influenced by the inflammatory environment. (a) Survivin protein expression and quantification in total adipose tissue (AT), adipocyte fraction (AD) and ...read more
Survivin is predominantly expressed in obese hASCs and is influenced by the inflammatory environment. (a) Survivin protein expression and quantification in total adipose tissue (AT), adipocyte fraction (AD) and ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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BID Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse BID
Met1-Asp195
Accession # AAC71064
Specificity
Detects human and mouse BID, a fragment generated in cells treated with anti-Fas and the carboxyl‑terminal portion of recombinant BID generated by cleavage with Caspase 8 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BID
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 4 ug/10^6 cells
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF860 in the following applications:

Publications
Read Publications using
AF860 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BID Antibody [Unconjugated]

  • apoptic death agonist
  • BH3 interacting domain death agonist
  • BH3-interacting domain death agonist
  • BID isoform ES(1b)
  • BID isoform L(2)
  • BID isoform Si6
  • BID
  • desmocollin type 4
  • FP497
  • Human BID coding sequence
  • MGC15319
  • MGC42355
  • p22 BID
  • tbid

Background

BID is a 195 amino acid member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1). BID is a pro-apoptotic member that causes cytochrome c to be released from the mitochondria intermembrane space into the cytosol. In healthy cells BID is cytosolic. In response to Fas ligand or TNF, BID is cleaved by caspase-8 and it then relocates to the mitochondria outer membrane (2, 3). Cleavage of BID by caspase-8 generates a new N-terminal that contains a terminal glycine. It appears that the glycine is myristoylated and myristoylation serves to target BID to the mitochondria (4). BID may then interact with another pro-apoptotic Bcl-2 family member Bak (5). Interaction of BID with Bak causes altered mitochondrial membrane permeability. A 9‑13 amino acid stretch called the BH3 region (Bcl-2 homology region) appears to mediate the BID interaction with other Bcl-2 family members. BID is neutralized by binding to the anti-apoptotic member Bcl-xL.

  1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
  2. Luo, X., et al. (1998) Cell 94:481.
  3. Li, H. et al. (1998) Cell 94:491.
  4. Zha, J. et al. (2000) Science 290:1761.
  5. Wei, M.C. et al. (2000) Genes Dev. 14:2060.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BID Antibody (AF860)(38)

We have publications tested in 3 confirmed species: Human, Mouse, Arabidopsis thaliana.

We have publications tested in 2 applications: ICC, Western Blot.


Filter By Application
ICC
(1)
Western Blot
(22)
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Filter By Species
Human
(10)
Mouse
(11)
Arabidopsis thaliana
(1)
All Species
Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using AF860 Applications Species
Sakthivel, D;Brown-Suedel, AN;Keane, F;Huang, S;Sherry, KM;Charendoff, CI;Dunne, KP;Robichaux, DJ;Le, B;Shin, CS;Carisey, AF;Flanagan, JM;Bouchier-Hayes, L; Caspase-2 is essential for proliferation and self-renewal of nucleophosmin-mutated acute myeloid leukemia bioRxiv : the preprint server for biology 2023-05-30 [PMID: 37398413] (Western Blot, Human) Western Blot Human
N Bajalovic, YZ Or, ARE Woo, SH Lee, VCL Lin High Levels of Progesterone Receptor B in MCF-7 Cells Enable Radical Anti-Tumoral and Anti-Estrogenic Effect of Progestin Biomedicines, 2022-08-02;10(8):. 2022-08-02 [PMID: 36009407] (Western Blot, Human) Western Blot Human
LE Buitrago-M, S Marhenke, D Becker, R Geffers, T Itzel, A Teufel, H Jaeschke, A Lechel, K Unger, J Markovic, AD Sharma, JU Marquardt, M Saborowski, A Saborowski, A Vogel p53-independent induction of p21 fails to control regeneration and hepatocarcinogenesis in a murine liver injury model Cellular and Molecular Gastroenterology and Hepatology, 2021-01-21;0(0):. 2021-01-21 [PMID: 33484913] (Western Blot, Mouse) Western Blot Mouse
A Manara, Z Imanifard, L Fracasso, D Bellin, M Crimi Plants expressing murine pro-apoptotic protein Bid do not have enhanced PCD Bmc Research Notes, 2020-09-21;13(1):450. 2020-09-21 [PMID: 32957987] (Western Blot, Arabidopsis thaliana) Western Blot Arabidopsis thaliana
NM de Vasconc, N Van Opdenb, H Van Gorp, R Martín-Pér, A Zecchin, P Vandenabee, M Lamkanfi An Apoptotic Caspase Network Safeguards Cell Death Induction in Pyroptotic Macrophages Cell Rep, 2020-07-28;32(4):107959. 2020-07-28 [PMID: 32726624] (Western Blot, Mouse) Western Blot Mouse
T Liu, T Kuwana, H Zhang, MG Vander Hei, RA Lerner, DD Newmeyer Phenotypic selection with an intrabody library reveals an anti-apoptotic function of PKM2 requiring Mitofusin-1 PLoS Biol., 2019-06-10;17(6):e2004413. 2019-06-10 [PMID: 31181072] (Western Blot, Human) Western Blot Human
S Naghdi, WS Slovinsky, M Madesh, E Rubin, G Hajnóczky Mitochondrial fusion and Bid-mediated mitochondrial apoptosis are perturbed by alcohol with distinct dependence on its metabolism Cell Death Dis, 2018-10-09;9(10):1028. 2018-10-09 [PMID: 30301883] (Western Blot, Human) Western Blot Human
Christi T Salisbury-Ruf, Clinton C Bertram, Aurelia Vergeade, Daniel S Lark, Qiong Shi, Marlene L Heberling et al. Bid maintains mitochondrial cristae structure and function and protects against cardiac disease in an integrative genomics study eLife 2018-10-03 [PMID: 30281024]
NM de Vasconc, N Van Opdenb, H Van Gorp, E Parthoens, M Lamkanfi Single-cell analysis of pyroptosis dynamics reveals conserved GSDMD-mediated subcellular events that precede plasma membrane rupture Cell Death Differ., 2018-04-17;0(0):. 2018-04-17 [PMID: 29666477] (Western Blot, Mouse) Western Blot Mouse
Moritz M. Gaidt, Thomas S. Ebert, Dhruv Chauhan, Katharina Ramshorn, Francesca Pinci, Sarah Zuber et al. The DNA Inflammasome in Human Myeloid Cells Is Initiated by a STING-Cell Death Program Upstream of NLRP3 Cell 2017-11-16 [PMID: 29033128]
Show All 38 Publications.

Review for BID Antibody (AF860) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF860:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Human 01/09/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 21048194
SpeciesHuman

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FAQs for BID Antibody (AF860) (0)

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Secondary Antibodies

 

Isotype Controls

Additional BID Products

Blogs on BID.

Apoptosis and Necroptosis Part I: Important factors to identify both types of programmed cell death
Different types of cell death have classically been identified by discrete morphological changes. The hallmarks of apoptosis include cell shrinkage, nuclear fragmentation and membrane blebbing whereas necroptosis is characterized by cell swelling ...  Read full blog post.

The use of apoptosis antibodies and controls in cell death research
Apoptosis is a method of programmed cell death that is notably characterized by a morphological change in cellular nuclei and membrane appearance.  Not to be confused with necrosis, apoptosis is a pathway that is induced by a variety of factors tha...  Read full blog post.

The role of p53 in UV radiation DNA damage and subsequent tumorogenesis
p53, the protein product of the tp53 gene, is one of the most widely studied tumor suppressor proteins in cancer research.  p53 is unique in that it demonstrates both tumor suppressive and tumor progressive properties depending on whether it is fu...  Read full blog post.

active/cleaved Caspase 2 - Inducing apoptosis in response to cellular stress
Caspase-2 is a highly conserved member of the caspase family involved in the initiation and execution of apoptosis. While its function is still poorly understood, caspase-2 is thought to be important for apoptosis in response to DNA damage, bacteri...  Read full blog post.

Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival
B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co...  Read full blog post.

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Verified Customer
01/09/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol BID
Uniprot