PUMA Antibody [Alexa Fluor® 488]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alexa Fluor 488

PUMA Antibody [Alexa Fluor® 488] Summary

Immunogen
A synthetic peptide made to a region within the C-terminus of the human PUMA protein sequence (between residues 150-193). [UniProt# Q9BXH1]
Localization
Mitochondrial
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BBC3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
This PUMA antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. By WB a band at ~16 kDa is seen, representing the beta form of PUMA. Reactivity against the alpha form of PUMA is unknown.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PUMA Antibody [Alexa Fluor® 488]

  • BCL2 binding component 3
  • bcl-2-binding component 3
  • JFY1
  • JFY-1
  • p53 up-regulated modulator of apoptosis
  • PUMAFLJ42994

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PUMA Antibody (NB500-261AF488) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB500-261AF350
Alexa Fluor 405 NB500-261AF405
Alexa Fluor 488 NB500-261AF488
Alexa Fluor 532 NB500-261AF532
Alexa Fluor 594 NB500-261AF594
Alexa Fluor 647 NB500-261AF647
Alexa Fluor 700 NB500-261AF700
Alexa Fluor 750 NB500-261AF750
Biotin NB500-261B
DyLight 350 NB500-261UV
DyLight 405 NB500-261V
DyLight 488 NB500-261G
DyLight 550 NB500-261R
DyLight 594 NB500-261DL594
DyLight 650 NB500-261C
DyLight 680 NB500-261FR
DyLight 755 NB500-261IR
FITC NB500-261F
HRP NB500-261H
Janelia Fluor 549 NB500-261JF549
Janelia Fluor 646 NB500-261JF646

Additional PUMA Products

Bioinformatics Tool for PUMA Antibody (NB500-261AF488)

Discover related pathways, diseases and genes to PUMA Antibody (NB500-261AF488). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for PUMA Antibody (NB500-261AF488)

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Pathways for PUMA Antibody (NB500-261AF488)

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PTMs for PUMA Antibody (NB500-261AF488)

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Research Areas for PUMA Antibody (NB500-261AF488)

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Blogs on PUMA.

A link between Autophagy and Apoptosis: Chat with First Author Brent E. Fitzwalter
By Christina Towers, PhD. Autophagy is a cellular recycling process and most often a pro-survival mechanism that regulates cellular homeostasis. On the contrary, apoptosis is an extensively conserved and elaborate pr...  Read full blog post.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

Altered expression of BCL2 in cancer
Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease.  For example, too little cell death can promote cell overgrowth a...  Read full blog post.

IRE1 alpha dependent apoptotic-signaling pathway
Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

Understanding Noxa Regulation of Apoptosis
Noxa is a pro-apoptotic gene belonging to the Bcl2 protein family that is unique in that it contains only BH3 domain. The BH3-only subclass of proteins, including proteins like PUMA and Bim in addition to Noxa, regulate the remaining Bcl-2 family memb...  Read full blog post.

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Bioinformatics

Gene Symbol BBC3