p73 Antibody (5B429)

Images

 
Western Blot: p73 Antibody (5B429) [NBP2-24737] - Analysis of p73 in A) transfected cell lysate and B) HeLa cell lysate at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: p73 Antibody (5B429) [NBP2-24737] - p73 was detected in immersion fixed Hela human cell line using p73 [5B429] Monoclonal Antibody (Catalog # NBP2-24737) at 3 ug/mL for 3 hours ...read more
Immunohistochemistry-Paraffin: p73 Antibody (5B429) [NBP2-24737] - FFPE breast cancer probed with p73 antibody at 5 ug/ml. Human tissue was used for this test. Staining of tissues is enhanced by boiling tissue sections ...read more
Flow Cytometry: p73 Antibody (5B429) [NBP2-24737] - Detection of p73 in Human HeLa Cell Line. Human HeLa cell line was stained with Mouse Anti- p73 Monoclonal Antibody (Catalog # NBP2-24737, filled histogram), or Mouse ...read more
Flow (Intracellular): p73 Antibody (5B429) [NBP2-24737] - An intracellular stain was performed on HeLa cells with pan p73 (5B429) antibody NBP2-24737 (blue) and a matched isotype control NBP2-27287 (orange). Cells were ...read more
Simple Western: p73 Antibody (5B429) [NBP2-24737] - Lane view shows a specific band for p73 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
5B429
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

p73 Antibody (5B429) Summary

Immunogen
This antibody was raised against full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.
Specificity
p73 antibody (clone 5B429) recognizes all of the TAp73 isoforms i.e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674 / IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kDa and 45 kDa, have been described in literature (Sayan et al, 2008). p73 antibody clone 5B429 has also been referred to as 429 in the literature.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TP73
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Simple Western 1:50
  • Chromatin Immunoprecipitation 1:20-1:1000
  • Flow Cytometry 1 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:20-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 5-15 ug/ml
  • Immunohistochemistry-Paraffin 5-15 ug/ml
  • Immunoprecipitation 1:20-1:1000
  • CyTOF-ready
  • Flow (Intracellular)
Application Notes
Use in FLOW cytometry reported in scientific literature (PMID: 21152443).

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. This antibody is CyTOF ready.
Control
p73 Knockout HeLa Cell Lysate
Publications
Read Publications using
NBP2-24737 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for p73 Antibody (5B429)

  • p53-related protein
  • P73p53-like transcription factor
  • TA p73
  • TAp73
  • tumor protein p73

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-24737
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Applications: WB, Simple Western, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
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Publications for p73 Antibody (NBP2-24737)(53)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ChIP, FLOW, ICC/IF, IHC-Fr, IP, WB.


Filter By Application
ChIP
(3)
FLOW
(1)
ICC/IF
(5)
IHC-Fr
(1)
IP
(4)
WB
(22)
All Applications
Filter By Species
Human
(20)
Mouse
(6)
Rat
(1)
All Species
Showing Publications 1 - 10 of 53. Show All 53 Publications.
Publications using NBP2-24737 Applications Species
Kumar S, Talluri S, Pal J et al. Role of apurinic/apyrimidinic nucleases in the regulation of homologous recombination in myeloma: mechanisms and translational significance. Blood Cancer J. Sep 25 2018 [PMID: 30301882] (ChIP, Human) ChIP Human
Makino E, Gutmann V, Kosnopfel C et al. Melanoma cells resistant towards MAPK inhibitors exhibit reduced TAp73 expression mediating enhanced sensitivity to platinum-based drugs. Cell Death Dis Sep 11 2018 [PMID: 30206212] (WB, Human) WB Human
Yang L, Yang X, Tang Y et al. Inhibition of DNA-PK activity sensitizes A549 cells to X-ray irradiation by inducing the ATM-dependent DNA damage response Mol Med Rep. [PMID: 29620203] (WB, Human) WB Human
Papaspyropoulos A, Bradley L, Thapa A et al. RASSF1A uncouples Wnt from Hippo signalling and promotes YAP mediated differentiation via p73 Nat Commun 2018 Jan 30 [PMID: 29382819] (ICC/IF, Mouse) ICC/IF Mouse
Lee T, Pelletier J. Dependence of p53-deficient cells on the DHX9 DExH-box helicase. Oncotarget. 2017 May 09 [PMID: 28427210] (WB, Human) WB Human
Gonzalez R, De la Rosa AJ, Rufini A et al. Role of p63 and p73 isoforms on the cell death in patients with hepatocellular carcinoma submitted to orthotopic liver transplantation. PLoS ONE. Mar 28 2017 [PMID: 28350813] (ICC/IF, Human) ICC/IF Human
Prabhu VV, Hong B, Allen JE et al. Small molecule prodigiosin restores p53 tumor suppressor activity in chemoresistant colorectal cancer stem cells via c-Jun-mediated deltaNp73 inhibition and p73 activation. Cancer Res. 2016 Jan 12 [PMID: 26759239] (WB, Human) WB Human
Brahimi-Horn MC, Lacas-Gervais S, Adaixo R et al. Local mitochondrial-endolysosomal microfusion cleaves the voltage-dependent anion channel 1 to promote survival in hypoxia Mol. Cell. Biol. 2015 Feb 17 [PMID: 25691661] (WB, Human)

Details:
p73 antibody (Imgenex IMG-246) used for WB analysis of lysates from HeLa cells which were subjected or not to transfection with either control siRNA or TP73 siRNA in normoxia or hypoxia and analyzed by immunoblotting (Fig. S1D).
WB Human
Amelio I, Inoue S, Markert EK et al. TAp73 opposes tumor angiogenesis by promoting hypoxia-inducible factor 1alpha degradation. Proc. Natl. Acad. Sci. U.S.A. 2015 Jan 06 [PMID: 25421557] (IP, Human)

Details:
p73 antibody used for IP of endogenous p73 protein in experiments involving H1299 cell line.
IP Human
Venkatanarayan A, Raulji P, Norton W et al. IAPP-driven metabolic reprogramming induces regression of p53-deficient tumours in vivo Nature et al. 2014 Nov 17 [PMID: 25409149] (WB, Mouse) WB Mouse
Show All 53 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for p73 Antibody (NBP2-24737). (Showing 1 - 2 of 2 FAQs).

  1. Do you know if it also detects deltaN isoforms?
    • For product NPB2-24737, the immunogen was the full length human p73 protein isoform alpha. The epitope for this product has been mapped to the N-terminal region of this isoform. This region shares 100% homology with isoform delta, so it should also recognize this isoform as well.
  2. Also, have you guys mapped the epitope?
    • While we know the general region where the epitope lies, we have not yet mapped the exact epitope.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-24737AF350
Alexa Fluor 405 NBP2-24737AF405
Alexa Fluor 488 NBP2-24737AF488
Alexa Fluor 532 NBP2-24737AF532
Alexa Fluor 594 NBP2-24737AF594
Alexa Fluor 647 NBP2-24737AF647
Alexa Fluor 700 NBP2-24737AF700
Alexa Fluor 750 NBP2-24737AF750
Allophycocyanin NBP2-24737APC
Biotin NBP2-24737B
DyLight 350 NBP2-24737UV
DyLight 405 NBP2-24737V
DyLight 488 NBP2-24737G
DyLight 550 NBP2-24737R
DyLight 594 NBP2-24737DL594
DyLight 650 NBP2-24737C
DyLight 680 NBP2-24737FR
DyLight 755 NBP2-24737IR
FITC NBP2-24737F
HRP NBP2-24737H
Janelia Fluor 549 NBP2-24737JF549
Janelia Fluor 646 NBP2-24737JF646
PE NBP2-24737PE
PerCP NBP2-24737PCP

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Blogs on p73.

p73: An Important Tumor Suppressor Cousin of p53
p73 has been identified as a long-lost cousin of the p53 tumor suppressor protein. It has high homology with both p53 and with p63, a gene implicated in the maintenance of epithelial stem cells. The presence of significant homology between the DNA-bin...  Read full blog post.

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Bioinformatics

Gene Symbol TP73
Uniprot
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