p73 Antibody (5B429)


Western Blot: p73 Antibody (5B429) [NBP2-24737] - Analysis of p73 in A) transfected cell lysate and B) HeLa cell lysate at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: p73 Antibody (5B429) [NBP2-24737]
Immunohistochemistry-Paraffin: p73 Antibody (5B429) [NBP2-24737] - Formalin-fixed, paraffin-embedded breast cancer probed with p73 antibody at 5 ug/ml. Human tissue was used for this test. Staining of formalin-fixed ...read more
Flow Cytometry: p73 Antibody (5B429) [NBP2-24737] - Detection of p73 in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- p73 Monoclonal Antibody (Catalog # NBP2-24737, filled ...read more
Flow (Intracellular): p73 Antibody (5B429) [NBP2-24737] - An intracellular stain was performed on HeLa cells with pan p73 (5B429) antibody NBP2-24737 (blue) and a matched isotype control NBP2-27287 (orange). Cells were ...read more
Simple Western: p73 Antibody (5B429) [NBP2-24737] - Simple Western lane view shows a specific band for p73 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa ...read more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, IP, CyTOF-ready
1.0 mg/ml

p73 Antibody (5B429) Summary

This antibody was raised against full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.
p73 antibody (clone 5B429) recognizes all of the TAp73 isoforms i.e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674 / IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kDa and 45 kDa, have been described in literature (Sayan et al, 2008). p73 antibody clone 5B429 has also been referred to as 429 in the literature.
IgG1 Kappa
Protein G purified
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  • Western Blot 2 ug/ml
  • Simple Western 1:50
  • Chromatin Immunoprecipitation 1:20-1:1000
  • Flow Cytometry 1 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:20-1:1000
  • Immunohistochemistry-Frozen 5-15 ug/ml
  • Immunohistochemistry-Paraffin 5-15 ug/ml
  • Immunoprecipitation 1:20-1:1000
  • CyTOF-ready
  • Flow (Intracellular)
Application Notes
Use in FLOW cytometry reported in scientific literature ( PMID 21152443).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Read Publications using
NBP2-24737 in the following applications:

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for p73 Antibody (5B429)

  • p53-related protein
  • P73p53-like transcription factor
  • TA p73
  • TAp73
  • tumor protein p73


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p73 Antibody (NBP2-24737)(50)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ChIP, FLOW, ICC/IF, IHC-Fr, IP, WB.

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Showing Publications 1 - 10 of 50. Show All 50 Publications.
Publications using NBP2-24737 Applications Species
Papaspyropoulos A, Bradley L, Thapa A et al. RASSF1A uncouples Wnt from Hippo signalling and promotes YAP mediated differentiation via p73 Nat Commun 2018 Jan 30 [PMID: 29382819] (ICC/IF, Mouse) ICC/IF Mouse
Lee T, Pelletier J. Dependence of p53-deficient cells on the DHX9 DExH-box helicase. Oncotarget. 2017 May 09 [PMID: 28427210] (WB, Human) WB Human
Gonzalez R, De la Rosa AJ, Rufini A et al. Role of p63 and p73 isoforms on the cell death in patients with hepatocellular carcinoma submitted to orthotopic liver transplantation. PLoS ONE. Mar 28 2017 [PMID: 28350813] (ICC/IF, Human) ICC/IF Human
Prabhu VV, Hong B, Allen JE et al. Small molecule prodigiosin restores p53 tumor suppressor activity in chemoresistant colorectal cancer stem cells via c-Jun-mediated deltaNp73 inhibition and p73 activation. Cancer Res. 2016 Jan 12 [PMID: 26759239] (WB, Human) WB Human
Brahimi-Horn MC, Lacas-Gervais S, Adaixo R et al. Local mitochondrial-endolysosomal microfusion cleaves the voltage-dependent anion channel 1 to promote survival in hypoxia Mol. Cell. Biol. 2015 Feb 17 [PMID: 25691661] (WB, Human)

p73 antibody (Imgenex IMG-246) used for WB analysis of lysates from HeLa cells which were subjected or not to transfection with either control siRNA or TP73 siRNA in normoxia or hypoxia and analyzed by immunoblotting (Fig. S1D).
WB Human
Amelio I, Inoue S, Markert EK et al. TAp73 opposes tumor angiogenesis by promoting hypoxia-inducible factor 1alpha degradation. Proc. Natl. Acad. Sci. U.S.A. 2015 Jan 06 [PMID: 25421557] (IP, Human)

p73 antibody used for IP of endogenous p73 protein in experiments involving H1299 cell line.
IP Human
Venkatanarayan A, Raulji P, Norton W et al. IAPP-driven metabolic reprogramming induces regression of p53-deficient tumours in vivo Nature et al. 2014 Nov 17 [PMID: 25409149] (WB, Mouse) WB Mouse
Chowdhury P, Lin Ge, Liu K et al. Targeting TopBP1 at a convergent point of multiple oncogenic pathways for cancer therapy Nat Commun et al. 2014 Nov 17 [PMID: 25400145] (IP, WB, Human)

p73 antibody used for IP on lysates of breast cancer cell lines MDA-MB468 and BT549. The antibody also used for WB on H1299 cells and BT549 transfected or not with shp73 (Figure 7e).
IP, WB Human
Lu H, Yan C, Quan XX et al. CK2 Phosphorylates and Inhibits TAp73 Tumor Suppressor Function to Promote Expression of Cancer Stem Cell Genes and Phenotype in Head and Neck Cancer. Neoplasia. 2014 Oct 01 [PMID: 25379016] (WB, Human) WB Human
Zoric A, Horvat A, Slade N. Differential effects of diverse p53 isoforms on TAp73 transcriptional activity and apoptosis. Carcinogenesis. 2013 Mar [PMID: 23188674]
Show All 50 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for p73 Antibody (NBP2-24737). (Showing 1 - 2 of 2 FAQs).

  1. Do you know if it also detects deltaN isoforms?
    • For product NPB2-24737, the immunogen was the full length human p73 protein isoform alpha. The epitope for this product has been mapped to the N-terminal region of this isoform. This region shares 100% homology with isoform delta, so it should also recognize this isoform as well.
  2. Also, have you guys mapped the epitope?
    • While we know the general region where the epitope lies, we have not yet mapped the exact epitope.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-24737AF405
Alexa Fluor 488 Labeled NBP2-24737AF488
Alexa Fluor 647 Labeled NBP2-24737AF647
Alexa Fluor 700 Labeled NBP2-24737AF700
Allophycocyanin Labeled NBP2-24737APC
Biotin Labeled NBP2-24737B
DyLight 350 Labeled NBP2-24737UV
DyLight 405 Labeled NBP2-24737V
DyLight 488 Labeled NBP2-24737G
DyLight 550 Labeled NBP2-24737R
DyLight 650 Labeled NBP2-24737C
DyLight 680 Labeled NBP2-24737FR
DyLight 755 Labeled NBP2-24737IR
HRP Labeled NBP2-24737H
PE Labeled NBP2-24737PE
PerCP Labeled NBP2-24737PCP

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Blogs on p73.

p73: An Important Tumor Suppressor Cousin of p53
p73 has been identified as a long-lost cousin of the p53 tumor suppressor protein. It has high homology with both p53 and with p63, a gene implicated in the maintenance of epithelial stem cells. The presence of significant homology between the DNA-bin...  Read full blog post.

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Gene Symbol TP73