p73 Antibody (5B429) - BSA Free

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Western Blot: p73 Antibody (5B429) [NBP2-24737] - Consequences of DHX9 knockdown on protein levels of p53 targets in HCT116 p53+/+ and p53-/- cells. Western blot analysis of p53 transcriptional targets in HCT116 p53+/+ ...read more
Immunocytochemistry/ Immunofluorescence: p73 Antibody (5B429) [NBP2-24737] - p73 was detected in immersion fixed Hela human cell line using p73 [5B429] Monoclonal Antibody (Catalog # NBP2-24737) at 3 ug/mL for 3 hours ...read more
Immunohistochemistry-Paraffin: p73 Antibody (5B429) [NBP2-24737] - FFPE breast cancer probed with p73 antibody at 5 ug/ml. Human tissue was used for this test. Staining of tissues is enhanced by boiling tissue sections ...read more
Flow Cytometry: p73 Antibody (5B429) [NBP2-24737] - Detection of p73 in Human HeLa Cell Line. Human HeLa cell line was stained with Mouse Anti- p73 Monoclonal Antibody (Catalog # NBP2-24737, filled histogram), or Mouse ...read more
Western Blot: p73 Antibody (5B429) [NBP2-24737] - Analysis of p73 in A) transfected cell lysate and B) HeLa cell lysate at 2 ug/ml.
Flow (Intracellular): p73 Antibody (5B429) [NBP2-24737] - An intracellular stain was performed on HeLa cells with pan p73 (5B429) antibody NBP2-24737 (blue) and a matched isotype control NBP2-27287 (orange). Cells were ...read more
Simple Western: p73 Antibody (5B429) [NBP2-24737] - Lane view shows a specific band for p73 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IP, ChIP, CyTOF-ready
Clone
5B429
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

p73 Antibody (5B429) - BSA Free Summary

Immunogen
This antibody was raised against full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.
Specificity
p73 antibody (clone 5B429) recognizes all of the TAp73 isoforms i.e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674 / IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kDa and 45 kDa, have been described in literature (Sayan et al, 2008).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TP73
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 1:20-1:1000
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry 1 ug/mL. Use reported in scientific literature (PMID 21152443)
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 5-15 ug/ml
  • Immunohistochemistry-Paraffin 5-15 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Simple Western 1:25
  • Western Blot 2 ug/ml
Application Notes
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. This antibody is CyTOF ready.
Publications
Read Publications using
NBP2-24737 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for p73 Antibody (5B429) - BSA Free

  • p53-related protein
  • P73p53-like transcription factor
  • TA p73
  • TAp73
  • tumor protein p73

Background

p73 is a transcription factor that shares a significant amino acid identity with p53 and p63 (1). p73 and p63 can both activate p53-responsive genes and induce apoptosis upon over expression (2) Unlike p53, p73 is not induced by DNA damage and is not a target for inactivation by viral oncoproteins (3). Similar to p63, p73 has an additional coding region in the C-terminus (not found in p53) that can undergo complex alternative splicing giving rise to 3 splice forms (alpha, beta, and gamma) (4). Activation of p73 requires the presence of functional c-Abl. It has also be found that mutant p53 can bind to and inactivate p73 (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP, ChIP, CyTOF-ready
Supplier Logo

Publications for p73 Antibody (NBP2-24737)(60)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: Chemotaxis, FLOW, ICC/IF, IHC, IHC-Fr, IP, WB.


Filter By Application
Chemotaxis
(4)
FLOW
(1)
ICC/IF
(6)
IHC
(1)
IHC-Fr
(1)
IP
(4)
WB
(26)
All Applications
Filter By Species
Human
(24)
Mouse
(6)
Rat
(1)
All Species
Showing Publications 1 - 10 of 60. Show All 60 Publications.
Publications using NBP2-24737 Applications Species
Acton A, Placzek WJ Myeloid Cell Leukemia 1 Small Molecule Inhibitor S63845 Synergizes with Cisplatin in Triple-Negative Breast Cancer Cancers 2023-09-08 [PMID: 37760451] (WB, Human) WB Human
Suzuki K, Tange M, Yamagishi R et al. SMG6 regulates DNA damage and cell survival in Hippo pathway kinase LATS2-inactivated malignant mesothelioma Cell death discovery 2022-11-05 [PMID: 36335095] (ICC/IF, Human) ICC/IF Human
Malik N, Yan H, Yang HH et al. CBFB cooperates with p53 to maintain TAp73 expression and suppress breast cancer PLoS genetics 2021-05-01 [PMID: 33945523] (WB, Human) WB Human
Widden H, Kaczmarczyk A, Subedi A et al. MCL1 binds and negatively regulates the transcriptional function of tumor suppressor p73 Cell Death Dis 2020-11-03 [PMID: 33144577] (WB) WB
Wang C, Teo CR, Sabapathy K p53-Related Transcription Targets of TAp73 in Cancer Cells-Bona Fide or Distorted Reality? Int J Mol Sci 2020-02-17 [PMID: 32079264] (Chemotaxis) Chemotaxis
Oh ST, Stark A, Reichrath J. The p53 Signalling Pathway in Cutaneous Basal Cell Carcinoma: An Immunohistochemical Description Acta Dermato Venereologica 2020-03-31 [PMID: 32052850] (IHC) IHC
Kumar S, Talluri S, Pal J et al. Role of apurinic/apyrimidinic nucleases in the regulation of homologous recombination in myeloma: mechanisms and translational significance. Blood Cancer J. 2018-09-25 [PMID: 30301882] (Chemotaxis, Human) Chemotaxis Human
Makino E, Gutmann V, Kosnopfel C et al. Melanoma cells resistant towards MAPK inhibitors exhibit reduced TAp73 expression mediating enhanced sensitivity to platinum-based drugs. Cell Death Dis 2018-09-11 [PMID: 30206212] (WB, Human) WB Human
Yang L, Yang X, Tang Y et al. Inhibition of DNA-PK activity sensitizes A549 cells to X-ray irradiation by inducing the ATM-dependent DNA damage response Mol Med Rep. [PMID: 29620203] (WB, Human) WB Human
Papaspyropoulos A, Bradley L, Thapa A et al. RASSF1A uncouples Wnt from Hippo signalling and promotes YAP mediated differentiation via p73 Nat Commun 2018-01-30 [PMID: 29382819] (ICC/IF, Mouse) ICC/IF Mouse
Show All 60 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p73 Antibody (NBP2-24737). (Showing 1 - 2 of 2 FAQs).

  1. Do you know if it also detects deltaN isoforms?
    • For product NPB2-24737, the immunogen was the full length human p73 protein isoform alpha. The epitope for this product has been mapped to the N-terminal region of this isoform. This region shares 100% homology with isoform delta, so it should also recognize this isoform as well.
  2. Also, have you guys mapped the epitope?
    • While we know the general region where the epitope lies, we have not yet mapped the exact epitope.

Secondary Antibodies

 

Isotype Controls

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Blogs on p73.

p73: An Important Tumor Suppressor Cousin of p53
p73 has been identified as a long-lost cousin of the p53 tumor suppressor protein. It has high homology with both p53 and with p63, a gene implicated in the maintenance of epithelial stem cells. The presence of significant homology between the DNA-bin...  Read full blog post.

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Bioinformatics

Gene Symbol TP73
Uniprot