MDM2/HDM2 Antibody (SMP14)

Images

 
Western Blot: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - Daudi whole cell protein was separated by SDS-PAGE on a 7.5% gel and transferred to PVDF membrane. The membrane was probed with anti-MDM2 antibody at 2 ug/ml and ...read more
Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - IHC analysis of formalin fixed paraffin embedded tissue section of human breast cancer xenograft using MDM2/HDM2 antibody (clone SMP14) at 1:100 ...read more
Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - IHC analysis of formalin fixed paraffin embedded tissue section of human breast cancer xenograft using MDM2/HDM2 antibody (clone SMP14) at 1:100 ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P, IP
Clone
SMP14
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

MDM2/HDM2 Antibody (SMP14) Summary

Immunogen
Synthetic peptide C-SRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2/HDM2. [UniProt# Q00987]
Epitope
Amino acids 154 - 167.
Localization
Nuclear and cytoplasmic (expressed predominantly in the nucleoplasm).
Specificity
The SMP14 monoclonal antibody also recognises a peptide epitope around Thr-216 of murine MDM2 (when Thr-216 is unphosphorylated) see Zhang & Prives, 2001 (PMID: 11359766) for further details. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MDM2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen 1:200-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Immunohistochemistry - Frozen and Paraffin, Immunoprecipitation and Western blot. This antibody has also been reported useful in IHC on resin tissue sections. Staining of formalin-fixed paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Control
MDM2/HDM2 Lysate (NBP2-64834)
Reviewed Applications
Read 1 Review rated 4
using
NB100-2736 in the following applications:

Publications
Read Publications using
NB100-2736 in the following applications:

  • IHC
    2 publications
  • 1 publication
  • 1 publication
  • IP
    3 publications
  • WB
    12 publications

Reactivity Notes

Human. Rat reactivity reported in scientific literature (PMID: 15625077).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for MDM2/HDM2 Antibody (SMP14)

  • ACTFS
  • HDM2
  • HDMX
  • MDM2 oncogene, E3 ubiquitin protein ligase
  • MDM2

Background

MDM2, also known as HDM2, is a protein of the E3 ubiquitin-ligase family. MDM2 is found predominantly in the nucleus, most localized to the nucleoplasm. MDM2 is over expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer. MDM2 ubiquinates or contributes to the ubiquination of a variety of proteins, including p53, thereby contributing to their proteosomal degradation. MDM2's interaction with p53 is inhibitory, causing an arrest of the cell cycle and deregulation of apoptosis, which explains MDM2's presence in many cancers. MDM2 is induced by damage to the DNA, and as a result has many isoforms, most of which are present exclusively in cancerous tissues, and non-existent in normal tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-46085
Species: Hu
Applications: WB, IHC
H00027065-M01
Species: Hu, Rt
Applications: WB, ELISA
NBP2-67484
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-31308
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-P
NB100-556
Species: Hu
Applications: WB, IP
NB100-56098
Species: Hu, Mu, Ca
Applications: WB, IHC, IHC-P, IP
NBP1-28566
Species: Hu, Mu, Rt, Bv, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP
NB100-56888
Species: Hu, Mu, Rt, Ca, Eq
Applications: WB
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NB600-335
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
NBP2-66154
Species: Hu
NBP2-37474
Species: Hu, Mu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
NBP2-24695
Species: Hu
Applications: WB, IHC-P
NBP1-89150
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P

Publications for MDM2/HDM2 Antibody (NB100-2736)(17)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
IHC
(2)
IHC-Fr
(1)
IHC-P
(1)
IP
(3)
WB
(12)
All Applications
Filter By Species
Human
(13)
Mouse
(1)
Rat
(2)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB100-2736 Applications Species
Ramos GV, Cruz A, Silva WJ et al. Thyroid hormone upregulates MDM2 in rat type i fiber: implications for skeletal muscle mass regulation Acta Physiol (Oxf). 2017 Nov 27 [PMID: 29178319] (Rat) Rat
Joshi Y, Soria MG, Quadrato G et al. The MDM4/MDM2-p53-IGF1 axis controls axonal regeneration, sprouting and functional recovery after CNS injury. Brain. 2015 May 16 [PMID: 25981963] (WB, IHC-Fr, Mouse) WB, IHC-Fr Mouse
Inaba H, Kuboniwa M, Sugita H et al. Identification of Signaling Pathways Mediating Cell Cycle Arrest and Apoptosis Induced by Porphyromonas gingivalis in Human Trophoblasts Infect Immun 2012 Aug [PMID: 22689813] (WB, Human) WB Human
de Freitas Mda C, Ramalho LM, Xavier FC et al. p53 and MDM2 protein expression in actinic cheilitis. J Appl Oral Sci. 2008 Nov-Dec [PMID: 19082401] (IHC, Human) IHC Human
Chen D, Zhang Z, Li M, Wang W et al. Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function. Oncogene. 2007 Aug 2 [PMID: 17310983] (WB, Human) WB Human
Stevenson LF, Sparks A, Allende-Vega N et al. The deubiquitinating enzyme USP2a regulates the p53 pathway by targeting Mdm2. EMBO J. 2007 Feb 21 [PMID: 17290220] (IP, Human) IP Human
Paajarvi G, Roudier E, Crisby M et al. HMG-CoA reductase inhibitors, statins, induce phosphorylation of Mdm2 and attenuate the p53 response to DNA damage. FASEB J. 2005 Mar [PMID: 15625077] (IHC, Rat) IHC Rat
Minsky N, Oren M. The RING domain of Mdm2 mediates histone ubiquitylation and transcriptional repression. Mol Cell. 2004 Nov 19 [PMID: 15546622] (WB, IP, Human) WB, IP Human
Menendez S, Higgins M, Berkson RG et al. Nuclear export inhibitor leptomycin B induces the appearance of novel forms of human Mdm2 protein. Br J Cancer. 2003 Feb 24 [PMID: 12592381] (WB, Human) WB Human
Goldberg Z, Vogt Sionov R,Berger M. Tyrosine phosphorylation of Mdm2 by c-Abl: implications for p53 regulation. EMBO J. 2002 Jul 15 [PMID: 12110584] (WB, Human) WB Human
Show All 17 Publications.

Review for MDM2/HDM2 Antibody (NB100-2736) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-2736:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 09/08/2010
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MDM2/HDM2 Antibody (NB100-2736). (Showing 1 - 1 of 1 FAQs).

  1. I would like to know where I can expect to see the signals with NB100-2736 in WB. After checking the information from UniProt, the MW of MDM2 seems to be 55 KDa. However, it may be changed by phosphorylation and Ubl conjugation. Is it the reason why the image on the product page showing the band at 100 KDa?
    • This product is a bit tricky as the protein is especially prone to forming protein complexes with other proteins; specifically 53/TP53, TP73/p73, RBL5 and RP11. Due to these interactions, it is common for this protein to be detected at a much higher weight than the expected weight given in Uniprot. In our testing, this protein usually detects a band at 100kDa. It is also possible to detect lower bands at 55 and 30kDa.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2736AF405
Alexa Fluor 488 Labeled NB100-2736AF488
Alexa Fluor 647 Labeled NB100-2736AF647
Alexa Fluor 700 Labeled NB100-2736AF700
Biotin Labeled NB100-2736B
DyLight 350 Labeled NB100-2736UV
DyLight 405 Labeled NB100-2736V
DyLight 488 Labeled NB100-2736G
DyLight 550 Labeled NB100-2736R
DyLight 650 Labeled NB100-2736C
DyLight 680 Labeled NB100-2736FR
DyLight 755 Labeled NB100-2736IR
HRP Labeled NB100-2736H

Additional Array Products

Bioinformatics Tool for MDM2/HDM2 Antibody (NB100-2736)

Discover related pathways, diseases and genes to MDM2/HDM2 Antibody (NB100-2736). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MDM2/HDM2 Antibody (NB100-2736)

Discover more about diseases related to MDM2/HDM2 Antibody (NB100-2736).
 

Pathways for MDM2/HDM2 Antibody (NB100-2736)

View related products by pathway.

PTMs for MDM2/HDM2 Antibody (NB100-2736)

Learn more about PTMs related to MDM2/HDM2 Antibody (NB100-2736).
 

Research Areas for MDM2/HDM2 Antibody (NB100-2736)

Find related products by research area.

Blogs on MDM2/HDM2

There are no specific blogs for MDM2/HDM2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Anonymous
09/08/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol MDM2
Entrez
Uniprot