Bim Antibody - BSA Free


Independent Antibodies: Western Blot: Bim Antibody [NBP1-76963] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, more
Immunocytochemistry/ Immunofluorescence: Bim Antibody [NBP1-76963] - K562 cells with at 20 ug/mL.
Immunohistochemistry-Paraffin: Bim Antibody [NBP1-76963] - Human skin cancer cells with Bim antibody at 20 ug/ml.
Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Rat Myeloma Cell line:Loading: 15 ug of rat myeloma YB2/0 cell lysate per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% more
Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in HeLa Cells. Loading: 15 ug of lysate per lane. Antibodies: Bim NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat more
Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Human Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat more
Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ELISA, ICC/IF, IHC
BSA Free
1 mg/ml
Validated by:

Independent Antibodies


Order Details

Bim Antibody - BSA Free Summary

Antibody was raised against a 20 amino acid peptide corresponding to amino acids near the amino terminus of human Bim. The immunogen is located within the first 50 amino acids of Bim. Amino Acid Squence: AERPPQLRPGAPTSLQTEPQ
Human BIM has 3 isoforms, including isoform EL (198aa, 22kD), isoform L (138aa, 16kD) and isoform S (108aa, 13kD). Mouse BIM has 3 isoforms, including isoform EL (196aa, 22kD), isoform L (140aa, 16kD) and isoform S (110aa, 13kD). Rat BIM has 4 isoforms, including isoform BOD-L (196aa, 22kD), isoform L (140aa, 16kD) and isoform BOD-M (110aa, 13kD). NBP1-76963 can detect all three isoforms.
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 20 ug/ml
  • Immunohistochemistry-Paraffin 20 ug/ml
  • Simple Western
  • Western Blot 0.5 ug/mL
Application Notes
Simple Western reported by an internal validation. Separated by Size-All, antibody dilution of 1:50; matrix was 12-230 kDa.
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Bim Peptide (NBP1-76963PEP)
Read Publications using
NBP1-76963 in the following applications:

  • WB
    5 publications

Reactivity Notes

The antigen sequence is identical to that of mouse and differs from that of rat by one amino acid.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1 mg/ml
Peptide affinity purified

Alternate Names for Bim Antibody - BSA Free

  • BAM
  • bcl-2 interacting mediator of cell death
  • bcl-2 interacting protein Bim
  • Bcl2-interacting mediator of cell death
  • bcl2-L-11
  • BCL2-like 11 (apoptosis facilitator)
  • bcl-2-like protein 11
  • bcl-2-related ovarian death agonist
  • BIM-alpha6
  • BIM-beta6
  • BIM-beta7
  • BimEL
  • BimL


Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat. Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Bim Antibody (NBP1-76963)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.

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Showing Publications 1 - 8 of 8.
Publications using NBP1-76963 Applications Species
Imbaby S, Elkholy SE, Faisal S et al. The GSTP1/MAPKs/BIM/SMAC modulatory actions of nitazoxanide: Bioinformatics and experimental evidence in subcutaneous solid Ehrlich carcinoma-inoculated mice Life sciences 2023-02-22 [PMID: 36822315] (WB, Mouse) WB Mouse
Luan Q, Jin L, Jiang CC et al. RIPK1 regulates survival of human melanoma cells upon endoplasmic reticulum stress through autophagy Autophagy. 2015-05-27 [PMID: 26018731] (WB, Human) WB Human
Wang Y, Lv J, Cheng Y et al. Apoptosis induced by Ginkgo biloba (EGb761) in melanoma cells is Mcl-1-dependent PLoS One 2015-04-11 [PMID: 25860257] (WB, Human)

Citation using the Non-Recombinant Monoclonal format of this antibody.
WB Human
Wroblewski D, Jiang CC, Croft A et al. OBATOCLAX and ABT-737 induce ER stress responses in human melanoma cells that limit induction of apoptosis PLoS One 2013-12-25 [PMID: 24367627] (WB, Human)

Citation using the Non-Recombinant Monoclonal format of this antibody.
WB Human
Ramos SJ, Hernandez JB, Gatzka M, Walsh CM. Enhanced T cell apoptosis within Drak2-deficient mice promotes resistance to autoimmunity. J Immunol. 2008-12-01 [PMID: 19017949] (WB, Mouse)

WB (mouse splenic T cells), Fig. 3G.
WB Mouse
Zhang LJ, Hao YZ, Hu CS et al. Inhibition of apoptosis facilitates necrosis induced by cisplatin in gastric cancer cells Anticancer Drugs 2008-01-08 [PMID: 18176112]

Citation using the Non-Recombinant Monoclonal and Biotin format of this antibody.
Castro JE, Prada CE, Aguillon RA et al. Thymidine-phosphorothioate oligonucleotides induce activation and apoptosis of CLL cells independently of CpG motifs or BCL-2 gene interference. Leukemia. 2006-04-01 [PMID: 16498393]
Zhang XD, Gillespie SK, Borrow JM, Hersey P. The histone deacetylase inhibitor suberic bishydroxamate regulates the expression of multiple apoptotic mediators and induces mitochondria-dependent apoptosis of melanoma cells. Mol Cancer Ther. 2004-04-01 [PMID: 15078986]

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Bim Antibody (NBP1-76963). (Showing 1 - of FAQs).

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    Blogs on Bim.

    Apoptosis and Necroptosis Part I: Important factors to identify both types of programmed cell death
    Different types of cell death have classically been identified by discrete morphological changes. The hallmarks of apoptosis include cell shrinkage, nuclear fragmentation and membrane blebbing whereas necroptosis is characterized by cell swelling ...  Read full blog post.

    Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
    Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

    Altered expression of BCL2 in cancer
    Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease.  For example, too little cell death can promote cell overgrowth a...  Read full blog post.

    IRE1 alpha dependent apoptotic-signaling pathway
    Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

    Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival
    B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co...  Read full blog post.

    Understanding Noxa Regulation of Apoptosis
    Noxa is a pro-apoptotic gene belonging to the Bcl2 protein family that is unique in that it contains only BH3 domain. The BH3-only subclass of proteins, including proteins like PUMA and Bim in addition to Noxa, regulate the remaining Bcl-2 family memb...  Read full blog post.

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    Gene Symbol BCL2L11