Bim Antibody

Images

 
Western Blot: Bim Antibody [NBP1-76963] - Analysis of Bim in K562 (K) and A549 (A) whole cell lysates with Bim antibody at 1 ug/mL.
Immunohistochemistry-Paraffin: Bim Antibody [NBP1-76963] - Human skin cancer cells with Bim antibody at 20 ug/ml.
Western Blot: Bim Antibody [NBP1-76963] - Detection of Bim in K562 (lane 1) and A549 (lane 2). 10 ug of protein was loaded per lane and this antibody was used at 2 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Bim Antibody Summary

Immunogen
Antibody was raised against a 20 amino acid peptide corresponding to amino acids near the amino terminus of human Bim. The immunogen is located within the first 50 amino acids of Bim.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCL2L11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 20 ug/ml
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Bim Peptide (NBP1-76963PEP)
Publications
Read Publications using
NBP1-76963 in the following applications:

  • WB
    4 publications

Reactivity Notes

The antigen sequence is identical to that of mouse and differs from that of rat by one amino acid.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Bim Antibody

  • BAM
  • bcl-2 interacting mediator of cell death
  • bcl-2 interacting protein Bim
  • Bcl2-interacting mediator of cell death
  • bcl2-L-11
  • BCL2-like 11 (apoptosis facilitator)
  • bcl-2-like protein 11
  • bcl-2-related ovarian death agonist
  • BIM-alpha6
  • BIM-beta6
  • BIM-beta7
  • BIMBOD
  • BimEL
  • BimL

Background

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat. Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-76963
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P

Publications for Bim Antibody (NBP1-76963)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(4)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-76963 Applications Species
Luan Q, Jin L, Jiang CC et al. RIPK1 regulates survival of human melanoma cells upon endoplasmic reticulum stress through autophagy Autophagy. 2015 May 27 [PMID: 26018731] (WB, Human) WB Human
Wang Y, Lv J, Cheng Y et al. Apoptosis Induced by Ginkgo biloba (EGb761) in Melanoma Cells Is Mcl-1-Dependent PLoS ONE 2015 Apr 11 [PMID: 25860257] (WB, Human)

Details:
Bim antibody was used for WB analysis of human melanoma cell lines Mel-RM and Mel-AT which were treated or not with Ginkgo biloba extract EGb761 for various time intervals (Fig 4).
WB Human
Wroblewski D, Jiang CC, Croft A et al. OBATOCLAX and ABT-737 Induce ER Stress Responses in Human Melanoma Cells that Limit Induction of Apoptosis. PLoS One 2013 Dec 19 [PMID: 24367627] (WB, Human) WB Human
Ramos SJ, Hernandez JB, Gatzka M, Walsh CM. Enhanced T cell apoptosis within Drak2-deficient mice promotes resistance to autoimmunity. J Immunol. 2008 Dec 1 [PMID: 19017949] (WB, Mouse)

Details:
WB (mouse splenic T cells), Fig. 3G.
WB Mouse
Zhang LJ, Hao YZ, Hu CS et al. Inhibition of apoptosis facilitates necrosis induced by cisplatin in gastric cancer cells. Anticancer Drugs. 2008 Feb [PMID: 18176112]

Details:
Antibodies cited [WB (human gastric adenocarcinoma cell lines SGG-7901 (Fig. 5 a,b) and BGC-823 (Fig. 5a)]: 1. IMG-349A (Noxa): Fig. 5 a,b. The antibody was SiRNA validated in Fig. 5b. 2. IMG-171 (Bim): Fig. 5a,b.
Castro JE, Prada CE, Aguillon RA et al. Thymidine-phosphorothioate oligonucleotides induce activation and apoptosis of CLL cells independently of CpG motifs or BCL-2 gene interference. Leukemia. 2006 Apr [PMID: 16498393]
Zhang XD, Gillespie SK, Borrow JM, Hersey P. The histone deacetylase inhibitor suberic bishydroxamate regulates the expression of multiple apoptotic mediators and induces mitochondria-dependent apoptosis of melanoma cells. Mol Cancer Ther. 2004 Apr [PMID: 15078986]

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Bim Antibody (NBP1-76963). (Showing 1 - of FAQs).

Secondary Antibodies

 

Isotype Controls

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Blogs on Bim.

Apoptosis and Necroptosis Part I: Important factors to identify both types of programmed cell death
Different types of cell death have classically been identified by discrete morphological changes. The hallmarks of apoptosis include cell shrinkage, nuclear fragmentation and membrane blebbing whereas necroptosis is characterized by cell swelling...  Read full blog post.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

Altered expression of BCL2 in cancer
Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease.  For example, too little cell death can promote cell overgrowth a...  Read full blog post.

IRE1 alpha dependent apoptotic-signaling pathway
Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival
B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co...  Read full blog post.

Understanding Noxa Regulation of Apoptosis
Noxa is a pro-apoptotic gene belonging to the Bcl2 protein family that is unique in that it contains only BH3 domain. The BH3-only subclass of proteins, including proteins like PUMA and Bim in addition to Noxa, regulate the remaining Bcl-2 family memb...  Read full blog post.

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Bioinformatics

Gene Symbol BCL2L11
Entrez
Uniprot