Noxa Antibody (114C307.1)

Images

 
Western Blot: Noxa Antibody (114C307.1) [NB600-1159] - Detection in RL-7 cells (a follicular lymphoma)
Immunocytochemistry/ Immunofluorescence: Noxa Antibody (114C307.1) [NB600-1159] - Analysis using Azide Free version of NB600-1159 at 1:10. HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were ...read more
Immunohistochemistry-Paraffin: Noxa Antibody (114C307.1) [NB600-1159] - Bouin-fixed, paraffin-embedded Chronic Lymphocytic Leukemia (CLL) xenograft stained with Noxa antibody (1:2000), peroxidase-conjugate and DAB ...read more
Flow Cytometry: Noxa Antibody (114C307.1) [NB600-1159] - An intracellular stain was performed on MCF7 cells with Noxa [114C307.1] Antibody NB600-1159AF700 (blue) and a matched isotype control (orange). Cells were fixed ...read more
Flow Cytometry: Noxa Antibody (114C307.1) [NB600-1159] - An intracellular stain was performed on HeLa cells with Noxa [114C307.1] Antibody NB600-1159AF700 (blue) and a matched isotype control (orange). Cells were fixed ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
114C307.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Noxa Antibody (114C307.1) Summary

Immunogen
Noxa Antibody was developed by immunizing mice with a fusion protein containing human Noxa.
Localization
Mitochondrion, also reported in Cytosol (PMID 21145489) & Nucleus (PMID 12879012).
Specificity
An ~80 kD background band may also be seen in Jurkat cell lysate.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PMAIP1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Flow Cytometry 1 ug / million cells
  • Immunocytochemistry/Immunofluorescence 1:20-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
Control
Noxa Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB600-1159 in the following applications:

Publications
Read Publications using
NB600-1159 in the following applications:

Reactivity Notes

Reactivity in rat is reported in scientific literature (Seda et al Hepatol Res. 2010, 40: 701-710)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Noxa Antibody (114C307.1)

  • adult T cell leukemia-derived PMA-responsive
  • APR
  • Immediate-early-response protein APR
  • NOXAPMA-induced protein 1
  • phorbol-12-myristate-13-acetate-induced protein 1
  • Protein Noxa

Background

Noxa, phorbol-12-myristate-13-acetate-induced protein 1, PMA-induced protein 1, PMAIP1 (Human 6 kDa and Mouse 11.6 kDa) is a Bcl2 homology 3 (BH3) domain containing protein which plays a role in the regulation of apoptosis, specifically a pro-apoptotic role. Human Noxa contains only one BH3 domain, while the mouse and rat proteins contain two BH3 domains. The human protein is encoded by Exon 1 and 3, with Exon 2 present only in two splice variants. Human Noxa splice variants lack a BH3 domain, have no known function, and are rapidly targeted for degradation (1).

Noxa mediated apoptosis may follow its transcriptional activation by p53 as part of the DNA damage response. However, Noxa expression may be induced by HIF-1 alpha under hypoxia, promoting apoptosis independently of p53 (1). Inhibition of the anti-apoptotic Bcl2 family member Myeloid cell leukemia-1 (Mcl-1) by Noxa, leads to the activation of pro-apoptotic Bcl2 homologous antagonist killer (Bak) and Bcl2 associated X (Bax) proteins, and apoptosis through the intrinsic mitochondrial pathway (2). Other BH3 only proteins such as Bim, Puma, Bad and Bid act via the same mechanism, targeting Mcl-1 and inducing Bak/Bax mediated mitochondrial outer membrane permeabilization (MOMP), cytochrome c release and caspase 9 activation (3). Overexpression of antiapoptotic Bcl2 family members is common in various types of cancer including prostate cancer. A recent study identified antiapoptotic Bcl2 proteins involved in the development of resistance towards the androgen receptor antagonist enzalutamide, which is used for the treatment of metastatic castration-resistant prostate cancer (mCRPC) (3).

References

1.Ploner, C., Kofler, R., & Villunger, A. (2008). Noxa: At the tip of the balance between life and death. Oncogene. https://doi.org/10.1038/onc.2009.46

2.Xiang, W., Yang, C. Y., & Bai, L. (2018). MCL-1 inhibition in cancer treatment. OncoTargets and Therapy. https://doi.org/10.2147/OTT.S146228

3.Pilling, A. B., & Hwang, C. (2019). Targeting prosurvival BCL2 signaling through Akt blockade sensitizes castration-resistant prostate cancer cells to enzalutamide. Prostate. https://doi.org/10.1002/pros.23843

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Noxa Antibody (NB600-1159)(70)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, WB, WB,IHC-P.


Filter By Application
ICC/IF
(2)
IHC
(1)
WB
(48)
WB,IHC-P
(1)
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Human
(33)
Mouse
(6)
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Showing Publications 1 - 10 of 70. Show All 70 Publications.
Publications using NB600-1159 Applications Species
Pilling AB, Hwang C Targeting prosurvival BCL2 signaling through Akt blockade sensitizes castration-resistant prostate cancer cells to enzalutamide Prostate Aug 1 2019 [PMID: 31228231] (WB, Human) WB Human
Gonzalez PS, O'Prey J, Cardaci S et al. Mannose impairs tumour growth and enhances chemotherapy. Nature. Nov 1 2018 [PMID: 30464341] (Mouse) Mouse
Minieri V, De Dominici M, Porazzi P et al. Targeting STAT5 or STAT5-regulated pathways suppresses leukemogenesis of Ph+ acute lymphoblastic leukemia. Cancer Res. Aug 28 2018 [PMID: 30154155] (WB, Human) WB Human
Gallagher SJ, Gunatilake D, Beaumont KA et al. HDAC inhibitors restore BRAF-inhibitor sensitivity by altering PI3K and survival signalling in a subset of melanoma Int. J. Cancer [PMID: 29210065] (WB, Human) WB Human
Antignani A, Segal D, Simon N et al. Essential role for Bim in mediating the apoptotic and antitumor activities of immunotoxins. Oncogene. Apr 24 2017 [PMID: 28436946] (WB, Mouse) WB Mouse
Zhang J, Huang K, O'Neill KL et al. Bax/Bak activation in the absence of Bid, Bim, Puma, and p53. Cell Death Dis Jun 17 2016 [PMID: 27310874] (WB, Human) WB Human
Heinemann A, Cullinane C, De Paoli-Iseppi R et al. Combining BET and HDAC inhibitors synergistically induces apoptosis of melanoma and suppresses AKT and YAP signaling Oncotarget. 2015 Jun 05 [PMID: 26087189] (WB, Human)

Details:
Noxa antibody clone 114C307.1 was used for WB application on total protein lysates from Patient-1-post and Me1007 cells, harvested after 24 h upon treatment with I-BET151 and/or LBH589 after 24 h (Figure 4C).
WB Human
Wang Y, Lv J, Cheng Y et al. Apoptosis Induced by Ginkgo biloba (EGb761) in Melanoma Cells Is Mcl-1-Dependent PLoS ONE 2015 Apr 11 [PMID: 25860257] (WB, Human)

Details:
Noxa antibody was used for WB analysis of human melanoma cell lines Mel-RM and Mel-AT which were treated or not with Ginkgo biloba extract EGb761 for various time intervals (Fig 4).
WB Human
Sigurdsson HH, Olesen CW, Dybboe R et al. Constitutively Active ErbB2 Regulates Cisplatin-induced Cell Death in Breast Cancer Cells via Pro- and Anti-apoptotic Mechanisms. Mol. Cancer Res. 2014 Aug 20 [PMID: 25143433] (WB, Human)

Details:
Noxa antibody (IMG-349A) used for WB on MCF-tTA-delta NErbB2 cells /delta NErbB2-expressing cells which express the truncated ErbB2 receptor under a tetracycline-off promoter and their corresponding vector controls MCF-tTA-pTRE cells treated or not with Cisplatin (Figure 4B). Antibody also used on siRNA-NOXA treated cells and WB data for NOXA knockdown is shown in Supplementary Figure S2C.
WB Human
Wei J, Noto JM, Zaika E et al. Bacterial CagA protein induces degradation of p53 protein in a p14ARF-dependent manner. Gut. 2014 Jul 30 [PMID: 25080447]
Show All 70 Publications.

Review for Noxa Antibody (NB600-1159) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB600-1159:
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Images Ratings Applications Species Date Details
Western Blot Noxa NB600-1159
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reviewed by:
Anonymous
WB Mouse 10/04/2011
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Summary

ApplicationWestern Blot
Sample TestedWEHI 7.2 mouse T cell lymphoma cell lysate, Sample Amount: 100ug
SpeciesMouse
Commentsbackground band just above 15kD that sometimes overtook the Noxa band. Recommend cutting the membrane just above 15kD to get rid of this problem before antibody incubation. transferred in CAPS buffer for 1 hr 15min at 25V

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:125, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse, Secondary Ab Dilution Ratio: 1:10,000
Secondary Manufacturer Cat#Amersham NA934

Details

Detection NotesDetection Method: ECL Plus, Exposure Time: 30 sec, Specific Bands: approximately 11kDa, Non-Specific Bands: just above the 15kD

Comments

Commentsbackground band just above 15kD that sometimes overtook the Noxa band. Recommend cutting the membrane just above 15kD to get rid of this problem before antibody incubation. transferred in CAPS buffer for 1 hr 15min at 25V

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Noxa Antibody (NB600-1159) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-1159AF350
Alexa Fluor 405 NB600-1159AF405
Alexa Fluor 488 NB600-1159AF488
Alexa Fluor 532 NB600-1159AF532
Alexa Fluor 594 NB600-1159AF594
Alexa Fluor 647 NB600-1159AF647
Alexa Fluor 700 NB600-1159AF700
Alexa Fluor 750 NB600-1159AF750
Biotin NBP2-24790
DyLight 350 NB600-1159UV
DyLight 405 NB600-1159V
DyLight 488 NB600-1159G
DyLight 550 NB600-1159R
DyLight 594 NB600-1159DL594
DyLight 650 NB600-1159C
DyLight 680 NB600-1159FR
DyLight 755 NB600-1159IR
FITC NB600-1159F
HRP NB600-1159H
Janelia Fluor 549 NB600-1159JF549
Janelia Fluor 646 NB600-1159JF646

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Blogs on Noxa.

NOXA - a BH3-only protein balancing cell death decisions
Noxa is a BH3-only protein involved in regulating cell death decisions. Noxa is a primary p53-response gene and is upregulated in response to p53 overexpression or DNA damage. Noxa can also be induced by alternative mechanisms including through a ...  Read full blog post.

Understanding Noxa Regulation of Apoptosis
Noxa is a pro-apoptotic gene belonging to the Bcl2 protein family that is unique in that it contains only BH3 domain. The BH3-only subclass of proteins, including proteins like PUMA and Bim in addition to Noxa, regulate the remaining Bcl-2 family memb...  Read full blog post.

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Recent Reviews

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Anonymous
10/04/2011
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PMAIP1
Entrez
Uniprot
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