Noxa Antibody (114C307.1)


Western Blot: Noxa Antibody (114C307.1) [NB600-1159] - Detection of Noxa antibody in A) 293 cells transfected with human Noxa, B) RL-7 cell (a follicular lymphoma) and C) T98G lysate using this antibody.
Immunocytochemistry/ Immunofluorescence: Noxa Antibody (114C307.1) [NB600-1159] - Noxa antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and more
Immunohistochemistry-Paraffin: Noxa Antibody (114C307.1) [NB600-1159] - Bouin-fixed, paraffin-embedded Chronic Lymphocytic Leukemia (CLL) xenograft stained with Noxa antibody (1:2000), peroxidase-conjugate and DAB more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
1.0 mg/ml

Noxa Antibody (114C307.1) Summary

Noxa Antibody was developed by immunizing mice with a fusion protein containing human Noxa.
Mitochondrion, also reported in Cytosol (PMID 21145489) & Nucleus (PMID 12879012).
An ~80 kD background band may also be seen in Jurkat cell lysate.
IgG1 Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified


Application Notes
Immunocytochemistry and Immunofluorescence; See Yakovlev et all (2003) for details.
Reviewed Applications
Read 1 Review rated 4
NB600-1159 in the following application:

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NB600-1159 in the following applications:

Reactivity Notes

Note- Reactivity with Rat is reported in scientific literature (Seda et al Hepatol Res. 2010, 40: 701-710)

Alternate Names for Noxa Antibody (114C307.1)

  • adult T cell leukemia-derived PMA-responsive
  • APR
  • Immediate-early-response protein APR
  • NOXAPMA-induced protein 1
  • phorbol-12-myristate-13-acetate-induced protein 1
  • Protein Noxa


Recently, a BH-3 only member of the Bcl-2 family have been identified in human and mouse and designated as Noxa (for damage) (1). The expression of the Noxa gene involves direct activation of its promoter by p53. Increased expression of Noxa protein occurs in normal thymocytes but not in p53-deficient thymocytes. Noxa cDNA codes for a 103-amino acid protein. The coimmunoprecipitation data suggest that Noxa protein may interact with proteins belonging to the Bcl-2 family, such as, Bcl-XL and Mcl-1. Oda et al (1) have also shown that blocking the endogenous Noxa induction results in the suppression of apoptosis. Treatment of cells with Noxa antisense oligonucleotides blocks radiation-induced apoptosis suggesting that Noxa may represent a mediator of p53-dependent apoptosis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Noxa Antibody (NB600-1159)(63)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, WB, WB,IHC-P.

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Showing Publications 1 - 10 of 63. Show All 63 Publications.
Publications using NB600-1159 Applications Species
Zhang J, Huang K, O'Neill KL et al. Bax/Bak activation in the absence of Bid, Bim, Puma, and p53. Cell Death Dis Jun 17 2016 12:00AM [PMID:27310874] (WB, Human) WB Human
Heinemann A, Cullinane C, De Paoli-Iseppi R et al. Combining BET and HDAC inhibitors synergistically induces apoptosis of melanoma and suppresses AKT and YAP signaling Oncotarget. 2015 Jun 05 [PMID:26087189] (WB, Human) WB Human
Wang Y, Lv J, Cheng Y et al. Apoptosis Induced by Ginkgo biloba (EGb761) in Melanoma Cells Is Mcl-1-Dependent PLoS ONE 2015 Apr 11 [PMID:25860257] (WB, Human) WB Human
Sigurdsson HH, Olesen CW, Dybboe R et al. Constitutively Active ErbB2 Regulates Cisplatin-induced Cell Death in Breast Cancer Cells via Pro- and Anti-apoptotic Mechanisms. Mol. Cancer Res. 2014 Aug 20 [PMID:25143433] (WB, Human) WB Human
Wei J, Noto JM, Zaika E et al. Bacterial CagA protein induces degradation of p53 protein in a p14ARF-dependent manner. Gut. 2014 Jul 30 [PMID:25080447]
Pang X, Zhang J, Lopez H et al. The Carboxyl-terminal Tail of Noxa Regulates the Stability of Noxa and Mcl-1. J. Biol. Chem. 2014 May 08 [PMID:24811167] (WB, Human) WB Human
Thorburn J, Andrysik Z, Staskiewicz L et al. Autophagy Controls the Kinetics and Extent of Mitochondrial Apoptosis by Regulating PUMA Levels. Cell Rep 4/1/2014 [PMID:24685133] (WB, Human) WB Human
Rao F, Cha J, Xu J et al. Inositol Pyrophosphates Mediate the DNA-PK/ATM-p53 Cell Death Pathway by Regulating CK2 Phosphorylation of Tti1/Tel2. Mol. Cell 2014 Mar 18 [PMID:24657168] (WB, Human) WB Human
Godbersen JC, Humphries LA, Danilova OV et al. The Nedd8-Activating Enzyme Inhibitor MLN4924 Thwarts Microenvironment-Driven NF-kB Activation and Induces Apoptosis in Chronic Lymphocytic Leukemia B Cells. Clin. Cancer Res. 2014 Mar 15 [PMID:24634471] (WB, Human) WB Human
Wroblewski D, Jiang CC, Croft A et al. OBATOCLAX and ABT-737 Induce ER Stress Responses in Human Melanoma Cells that Limit Induction of Apoptosis. PLoS One 2013 Dec 19 [PMID:24367627] (WB, Human) WB Human
Show All 63 Publications.

Reviews for Noxa Antibody (NB600-1159) (1) 41

Average Rating: 4
(Based on 1 reviews)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NB600-1159:
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Images Ratings Applications Species Date Details
Western Blot Noxa NB600-1159
reviewed by:
WB Mouse 10/04/2011


ApplicationWestern Blot
Sample TestedWEHI 7.2 mouse T cell lymphoma cell lysate, Sample Amount: 100ug
Commentsbackground band just above 15kD that sometimes overtook the Noxa band. Recommend cutting the membrane just above 15kD to get rid of this problem before antibody incubation. transferred in CAPS buffer for 1 hr 15min at 25V


Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:125, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse, Secondary Ab Dilution Ratio: 1:10,000
Secondary Manufacturer Cat#Amersham NA934


Detection NotesDetection Method: ECL Plus, Exposure Time: 30 sec, Specific Bands: approximately 11kDa, Non-Specific Bands: just above the 15kD


Commentsbackground band just above 15kD that sometimes overtook the Noxa band. Recommend cutting the membrane just above 15kD to get rid of this problem before antibody incubation. transferred in CAPS buffer for 1 hr 15min at 25V

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Noxa Antibody (NB600-1159) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB600-1159AF405
Alexa Fluor (R) 488 Labeled NB600-1159AF488
Alexa Fluor (R) 647 Labeled NB600-1159AF647
Alexa Fluor (R) 700 Labeled NB600-1159AF700
Allophycocyanin Labeled NB600-1159APC
Biotin Labeled NBP2-24790
DyLight 350 Labeled NB600-1159UV
DyLight 405 Labeled NB600-1159V
DyLight 405LS Labeled NB600-1159V3
DyLight 488 Labeled NB600-1159G
DyLight 550 Labeled NB600-1159R
DyLight 650 Labeled NB600-1159C
DyLight 680 Labeled NB600-1159FR
DyLight 755 Labeled NB600-1159IR
HRP Labeled NB600-1159H
PE Labeled NB600-1159PE
PerCP Labeled NB600-1159PCP

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Blogs on Noxa.

NOXA - a BH3-only protein balancing cell death decisions
Noxa is a BH3-only protein involved in regulating cell death decisions. Noxa is a primary p53-response gene and is upregulated in response to p53 overexpression or DNA damage. Noxa can also be induced by alternative mechanisms including through a ...  Read full blog post.

Understanding Noxa Regulation of Apoptosis
Noxa is a pro-apoptotic gene belonging to the Bcl2 protein family that is unique in that it contains only BH3 domain. The BH3-only subclass of proteins, including proteins like PUMA and Bim in addition to Noxa, regulate the remaining Bcl-2 family memb...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol PMAIP1

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