Noxa Antibody (114C307.1)

Images

 
Western Blot: Noxa Antibody (114C307.1) [NB600-1159] - Analysis using Azide Free version of NB600-1159. A) 293 cells transfected with human Noxa, B) RL-7 cell (a follicular lymphoma) and C) T98G lysate.
Immunocytochemistry/ Immunofluorescence: Noxa Antibody (114C307.1) [NB600-1159] - Analysis using Azide Free version of NB600-1159. HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with ...read more
Immunohistochemistry-Paraffin: Noxa Antibody (114C307.1) [NB600-1159] - Immunohistochemistry-Paraffin: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Bouin-fixed, paraffin-embedded Chronic Lymphocytic Leukemia ...read more
Flow Cytometry: Noxa Antibody (114C307.1) [NB600-1159] - An intracellular stain was performed on HeLa cells with Noxa [114C307.1] Antibody NB600-1159AF700 (blue) and a matched isotype control (orange). Cells were fixed ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
114C307.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Noxa Antibody (114C307.1) Summary

Immunogen
Noxa Antibody was developed by immunizing mice with a fusion protein containing human Noxa.
Localization
Mitochondrion, also reported in Cytosol (PMID 21145489) & Nucleus (PMID 12879012).
Specificity
An ~80 kD background band may also be seen in Jurkat cell lysate.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PMAIP1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Flow Cytometry 1 ug / million cells
  • Immunocytochemistry/Immunofluorescence 1:20-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
Immunocytochemistry and Immunofluorescence; See Yakovlev et all (2003) for details.
Control
Noxa Lysate (NBL1-14534)
Reviewed Applications
Read 1 Review rated 4
using
NB600-1159 in the following applications:

Publications
Read Publications using
NB600-1159 in the following applications:

Reactivity Notes

Note- Reactivity with Rat is reported in scientific literature (Seda et al Hepatol Res. 2010, 40: 701-710)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Noxa Antibody (114C307.1)

  • adult T cell leukemia-derived PMA-responsive
  • APR
  • Immediate-early-response protein APR
  • NOXAPMA-induced protein 1
  • phorbol-12-myristate-13-acetate-induced protein 1
  • Protein Noxa

Background

Recently, a BH-3 only member of the Bcl-2 family have been identified in human and mouse and designated as Noxa (for damage) (1). The expression of the Noxa gene involves direct activation of its promoter by p53. Increased expression of Noxa protein occurs in normal thymocytes but not in p53-deficient thymocytes. Noxa cDNA codes for a 103-amino acid protein. The coimmunoprecipitation data suggest that Noxa protein may interact with proteins belonging to the Bcl-2 family, such as, Bcl-XL and Mcl-1. Oda et al (1) have also shown that blocking the endogenous Noxa induction results in the suppression of apoptosis. Treatment of cells with Noxa antisense oligonucleotides blocks radiation-induced apoptosis suggesting that Noxa may represent a mediator of p53-dependent apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Noxa Antibody (NB600-1159)(66)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, WB, WB,IHC-P.


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ICC/IF
(2)
IHC
(1)
WB
(45)
WB,IHC-P
(1)
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Human
(30)
Mouse
(5)
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Showing Publications 1 - 10 of 66. Show All 66 Publications.
Publications using NB600-1159 Applications Species
Antignani A, Segal D, Simon N et al. Essential role for Bim in mediating the apoptotic and antitumor activities of immunotoxins. Oncogene. Apr 24 2017 [PMID: 28436946] (WB, Mouse) WB Mouse
Zhang J, Huang K, O'Neill KL et al. Bax/Bak activation in the absence of Bid, Bim, Puma, and p53. Cell Death Dis Jun 17 2016 [PMID: 27310874] (WB, Human) WB Human
Heinemann A, Cullinane C, De Paoli-Iseppi R et al. Combining BET and HDAC inhibitors synergistically induces apoptosis of melanoma and suppresses AKT and YAP signaling Oncotarget. 2015 Jun 05 [PMID: 26087189] (WB, Human)

Details:
Noxa antibody clone 114C307.1 was used for WB application on total protein lysates from Patient-1-post and Me1007 cells, harvested after 24 h upon treatment with I-BET151 and/or LBH589 after 24 h (Figure 4C).
WB Human
Wang Y, Lv J, Cheng Y et al. Apoptosis Induced by Ginkgo biloba (EGb761) in Melanoma Cells Is Mcl-1-Dependent PLoS ONE 2015 Apr 11 [PMID: 25860257] (WB, Human)

Details:
Noxa antibody was used for WB analysis of human melanoma cell lines Mel-RM and Mel-AT which were treated or not with Ginkgo biloba extract EGb761 for various time intervals (Fig 4).
WB Human
Sigurdsson HH, Olesen CW, Dybboe R et al. Constitutively Active ErbB2 Regulates Cisplatin-induced Cell Death in Breast Cancer Cells via Pro- and Anti-apoptotic Mechanisms. Mol. Cancer Res. 2014 Aug 20 [PMID: 25143433] (WB, Human)

Details:
Noxa antibody (IMG-349A) used for WB on MCF-tTA-delta NErbB2 cells /delta NErbB2-expressing cells which express the truncated ErbB2 receptor under a tetracycline-off promoter and their corresponding vector controls MCF-tTA-pTRE cells treated or not with Cisplatin (Figure 4B). Antibody also used on siRNA-NOXA treated cells and WB data for NOXA knockdown is shown in Supplementary Figure S2C.
WB Human
Wei J, Noto JM, Zaika E et al. Bacterial CagA protein induces degradation of p53 protein in a p14ARF-dependent manner. Gut. 2014 Jul 30 [PMID: 25080447]
Pang X, Zhang J, Lopez H et al. The Carboxyl-terminal Tail of Noxa Regulates the Stability of Noxa and Mcl-1. J. Biol. Chem. 2014 May 08 [PMID: 24811167] (WB, Human)

Details:
HeLa, Figs 1,2. The Noxa mAb detected Noxa at just below the 10 kDa marker. The Noxa mAb also detected two bands between the 37 and 50 kDa markers which was determined to be ubiquitylated NOXA by doing additional studies with a ubiquitin antibody and NOXA
WB Human
Thorburn J, Andrysik Z, Staskiewicz L et al. Autophagy Controls the Kinetics and Extent of Mitochondrial Apoptosis by Regulating PUMA Levels. Cell Rep 4/1/2014 [PMID: 24685133] (WB, Human) WB Human
Rao F, Cha J, Xu J et al. Inositol Pyrophosphates Mediate the DNA-PK/ATM-p53 Cell Death Pathway by Regulating CK2 Phosphorylation of Tti1/Tel2. Mol. Cell 2014 Mar 18 [PMID: 24657168] (WB, Human)

Details:
Mouse embryo fibroblasts, Fig 4A.
WB Human
Godbersen JC, Humphries LA, Danilova OV et al. The Nedd8-Activating Enzyme Inhibitor MLN4924 Thwarts Microenvironment-Driven NF-kB Activation and Induces Apoptosis in Chronic Lymphocytic Leukemia B Cells. Clin. Cancer Res. 2014 Mar 15 [PMID: 24634471] (WB, Human)

Details:
CLL leukemia cells, Figs 4D, 5C, 5D. Treatment with MLN4924 or Bortezombid led to upregulation of Noxa. The specificity of the Noxa Ab was validated with Noxa siRNA by WB in CLL cells (Fig 5C).
WB Human
Show All 66 Publications.

Review for Noxa Antibody (NB600-1159) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB600-1159:
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(1)
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Images Ratings Applications Species Date Details
Western Blot Noxa NB600-1159
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reviewed by:
Anonymous
WB Mouse 10/04/2011
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Summary

ApplicationWestern Blot
Sample TestedWEHI 7.2 mouse T cell lymphoma cell lysate, Sample Amount: 100ug
SpeciesMouse
Commentsbackground band just above 15kD that sometimes overtook the Noxa band. Recommend cutting the membrane just above 15kD to get rid of this problem before antibody incubation. transferred in CAPS buffer for 1 hr 15min at 25V

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:125, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse, Secondary Ab Dilution Ratio: 1:10,000
Secondary Manufacturer Cat#Amersham NA934

Details

Detection NotesDetection Method: ECL Plus, Exposure Time: 30 sec, Specific Bands: approximately 11kDa, Non-Specific Bands: just above the 15kD

Comments

Commentsbackground band just above 15kD that sometimes overtook the Noxa band. Recommend cutting the membrane just above 15kD to get rid of this problem before antibody incubation. transferred in CAPS buffer for 1 hr 15min at 25V

Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

FAQs for Noxa Antibody (NB600-1159) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-1159AF405
Alexa Fluor 488 Labeled NB600-1159AF488
Alexa Fluor 647 Labeled NB600-1159AF647
Alexa Fluor 700 Labeled NB600-1159AF700
Biotin Labeled NBP2-24790
DyLight 350 Labeled NB600-1159UV
DyLight 405 Labeled NB600-1159V
DyLight 488 Labeled NB600-1159G
DyLight 550 Labeled NB600-1159R
DyLight 650 Labeled NB600-1159C
DyLight 680 Labeled NB600-1159FR
DyLight 755 Labeled NB600-1159IR
HRP Labeled NB600-1159H

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Blogs on Noxa.

NOXA - a BH3-only protein balancing cell death decisions
Noxa is a BH3-only protein involved in regulating cell death decisions. Noxa is a primary p53-response gene and is upregulated in response to p53 overexpression or DNA damage. Noxa can also be induced by alternative mechanisms including through a ...  Read full blog post.

Understanding Noxa Regulation of Apoptosis
Noxa is a pro-apoptotic gene belonging to the Bcl2 protein family that is unique in that it contains only BH3 domain. The BH3-only subclass of proteins, including proteins like PUMA and Bim in addition to Noxa, regulate the remaining Bcl-2 family memb...  Read full blog post.

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Recent Reviews

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Anonymous
10/04/2011
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PMAIP1
Entrez
Uniprot