Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal

Images

 
Western Blot: Noxa Antibody (114C307.1) [NB600-1159] - Western blot analysis in RL-7 cells (a follicular lymphoma).
Immunocytochemistry/ Immunofluorescence: Noxa Antibody (114C307.1) [NB600-1159] - ICC/IF analysis using the azide-free version of NB600-1159 at 1:10. HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were ...read more
Immunohistochemistry-Paraffin: Noxa Antibody (114C307.1) [NB600-1159] - Bouin-fixed, paraffin-embedded Chronic Lymphocytic Leukemia (CLL) xenograft stained with Noxa antibody (1:2000), peroxidase-conjugate and DAB ...read more
Flow Cytometry: Noxa Antibody (114C307.1) [NB600-1159] - An intracellular stain was performed on Ntera2 cells with Noxa Antibody [114C307.1] NB600-1159 (blue) and a matched isotype control (orange). Cells were fixed ...read more
Flow Cytometry: Noxa Antibody (114C307.1) [NB600-1159] - Flow cytometry after an intracellular stain was performed on HeLa cells with Noxa [114C307.1] antibody NB600-1159AF700 (blue) and a matched isotype control ...read more
Flow Cytometry: Noxa Antibody (114C307.1) [NB600-1159] - Flow cytometry after an intracellular stain was performed on MCF7 cells with Noxa [114C307.1] antibody NB600-1159AF700 (blue) and a matched isotype control ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
114C307.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Non-Recombinant Monoclonal
Concentration
1.0 mg/ml

Order Details

View Available Formulations

Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal Summary

Immunogen
This Noxa antibody was developed by immunizing mice with a fusion protein containing human Noxa.
Localization
Mitochondrion, also reported in Cytosol (PMID 21145489) & Nucleus (PMID 12879012).
Specificity
An ~80 kD background band may also be seen in Jurkat cell lysate.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PMAIP1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/mL
  • Flow Cytometry 1 ug / million cells
  • Immunocytochemistry/Immunofluorescence 1:20 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 5 ug/mL
Theoretical MW
14.96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Noxa Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB600-1159 in the following applications:

Publications
Read Publications using
NB600-1159 in the following applications:

Reactivity Notes

Reactivity in rat is reported in scientific literature (Seda et al Hepatol Res. 2010, 40: 701-710). Rat reactivity reported in scientific literature (PMID:33031904).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal

  • adult T cell leukemia-derived PMA-responsive
  • APR
  • Immediate-early-response protein APR
  • NOXAPMA-induced protein 1
  • phorbol-12-myristate-13-acetate-induced protein 1
  • PMA-Induced Protein 1
  • Protein Noxa

Background

Noxa, phorbol-12-myristate-13-acetate-induced protein 1, PMA-induced protein 1, PMAIP1 (Human 6 kDa and Mouse 11.6 kDa) is a Bcl2 homology 3 (BH3) domain containing protein which plays a role in the regulation of apoptosis, specifically a pro-apoptotic role. Human Noxa contains only one BH3 domain, while the mouse and rat proteins contain two BH3 domains. The human protein is encoded by Exon 1 and 3, with Exon 2 present only in two splice variants. Human Noxa splice variants lack a BH3 domain, have no known function, and are rapidly targeted for degradation (1).

Noxa mediated apoptosis may follow its transcriptional activation by p53 as part of the DNA damage response. However, Noxa expression may be induced by HIF-1 alpha under hypoxia, promoting apoptosis independently of p53 (1). Inhibition of the anti-apoptotic Bcl2 family member Myeloid cell leukemia-1 (Mcl-1) by Noxa, leads to the activation of pro-apoptotic Bcl2 homologous antagonist killer (Bak) and Bcl2 associated X (Bax) proteins, and apoptosis through the intrinsic mitochondrial pathway (2). Other BH3 only proteins such as Bim, Puma, Bad and Bid act via the same mechanism, targeting Mcl-1 and inducing Bak/Bax mediated mitochondrial outer membrane permeabilization (MOMP), cytochrome c release and caspase 9 activation (3). Overexpression of antiapoptotic Bcl2 family members is common in various types of cancer including prostate cancer. A recent study identified antiapoptotic Bcl2 proteins involved in the development of resistance towards the androgen receptor antagonist enzalutamide, which is used for the treatment of metastatic castration-resistant prostate cancer (mCRPC) (3).

References

1.Ploner, C., Kofler, R., & Villunger, A. (2008). Noxa: At the tip of the balance between life and death. Oncogene. https://doi.org/10.1038/onc.2009.46

2.Xiang, W., Yang, C. Y., & Bai, L. (2018). MCL-1 inhibition in cancer treatment. OncoTargets and Therapy. https://doi.org/10.2147/OTT.S146228

3.Pilling, A. B., & Hwang, C. (2019). Targeting prosurvival BCL2 signaling through Akt blockade sensitizes castration-resistant prostate cancer cells to enzalutamide. Prostate. https://doi.org/10.1002/pros.23843

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Noxa Antibody (NB600-1159)(72)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, WB, WB,IHC-P.


Filter By Application
ICC/IF
(2)
IHC
(1)
WB
(49)
WB,IHC-P
(1)
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Human
(32)
Mouse
(7)
Rat
(1)
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Showing Publications 1 - 10 of 72. Show All 72 Publications.
Publications using NB600-1159 Applications Species
Zheng J, Zhuo L, Ran D et al. Cadmium induces apoptosis via generating reactive oxygen species to activate mitochondrial p53 pathway in primary rat osteoblasts Toxicology Oct 6 2020 [PMID: 33031904] (WB, Rat) WB Rat
Noxa (IMG-349) [WB, Fig4 (H1299 cells)].
Tseng HY, Dreyer J, Emran AA et al. Co-targeting bromodomain and extra-terminal proteins and MCL1 induces synergistic cell death in melanoma Int. J. Cancer Apr 5 2020 [PMID: 32249419]
Djajawi TM, Liu L, Gong JN et al. MARCH5 requires MTCH2 to coordinate proteasomal turnover of the MCL1:NOXA complex Cell Death Differ. Feb 24 2020 [PMID: 32094511] (WB, Mouse) WB Mouse
Pilling AB, Hwang C Targeting prosurvival BCL2 signaling through Akt blockade sensitizes castration-resistant prostate cancer cells to enzalutamide Prostate Aug 1 2019 [PMID: 31228231] (WB, Human) WB Human
Gonzalez PS, O'Prey J, Cardaci S et al. Mannose impairs tumour growth and enhances chemotherapy. Nature. Nov 1 2018 [PMID: 30464341] (Mouse) Mouse
Minieri V, De Dominici M, Porazzi P et al. Targeting STAT5 or STAT5-regulated pathways suppresses leukemogenesis of Ph+ acute lymphoblastic leukemia. Cancer Res. Aug 28 2018 [PMID: 30154155] (WB, Human) WB Human
Gallagher SJ, Gunatilake D, Beaumont KA et al. HDAC inhibitors restore BRAF-inhibitor sensitivity by altering PI3K and survival signalling in a subset of melanoma Int. J. Cancer [PMID: 29210065] (WB, Human) WB Human
Antignani A, Segal D, Simon N et al. Essential role for Bim in mediating the apoptotic and antitumor activities of immunotoxins. Oncogene. Apr 24 2017 [PMID: 28436946] (WB, Mouse) WB Mouse
Zhang J, Huang K, O'Neill KL et al. Bax/Bak activation in the absence of Bid, Bim, Puma, and p53. Cell Death Dis Jun 17 2016 [PMID: 27310874] (WB, Human) WB Human
Show All 72 Publications.

Review for Noxa Antibody (NB600-1159) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB600-1159:
Filter by Applications
WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot Noxa NB600-1159
Enlarge
4
reviewed by:
Anonymous
WB Mouse 10/04/2011
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Summary

ApplicationWestern Blot
Sample TestedWEHI 7.2 mouse T cell lymphoma cell lysate, Sample Amount: 100ug
SpeciesMouse
Commentsbackground band just above 15kD that sometimes overtook the Noxa band. Recommend cutting the membrane just above 15kD to get rid of this problem before antibody incubation. transferred in CAPS buffer for 1 hr 15min at 25V

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:125, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse, Secondary Ab Dilution Ratio: 1:10,000
Secondary Manufacturer Cat#Amersham NA934

Details

Detection NotesDetection Method: ECL Plus, Exposure Time: 30 sec, Specific Bands: approximately 11kDa, Non-Specific Bands: just above the 15kD

Comments

Commentsbackground band just above 15kD that sometimes overtook the Noxa band. Recommend cutting the membrane just above 15kD to get rid of this problem before antibody incubation. transferred in CAPS buffer for 1 hr 15min at 25V

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Noxa Antibody (NB600-1159) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Noxa.

NOXA - a BH3-only protein balancing cell death decisions
Noxa is a BH3-only protein involved in regulating cell death decisions. Noxa is a primary p53-response gene and is upregulated in response to p53 overexpression or DNA damage. Noxa can also be induced by alternative mechanisms including through a ...  Read full blog post.

Understanding Noxa Regulation of Apoptosis
Noxa is a pro-apoptotic gene belonging to the Bcl2 protein family that is unique in that it contains only BH3 domain. The BH3-only subclass of proteins, including proteins like PUMA and Bim in addition to Noxa, regulate the remaining Bcl-2 family memb...  Read full blog post.

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Recent Reviews

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5
0
4
1
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0
2
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1
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Anonymous
10/04/2011
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PMAIP1
Entrez
Uniprot