Alpha-TTP Antibody

Western Blot: Alpha-TTP Antibody [NBP2-14870] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with alpha TTP antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Alpha-TTP Antibody [NBP2-14870] - Analysis of methanol-fixed MCF-7, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: Alpha-TTP Antibody [NBP2-14870] - Paraffin-embedded gastric carcinoma; antibody dilution 1:500.

Western Blot: Alpha-TTP Antibody [NBP2-14870] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE; antibody diluted at 1:1000.

Western Blot: Alpha-TTP Antibody [NBP2-14870] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with alpha TTP antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Ha
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Alpha-TTP Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human alpha TTP. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TTPA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Alpha-TTP Antibody

• alpha-tocopherol transfer protein
• alphaTTP
• Alpha-TTP
• ataxia (Friedreich-like) with vitamin E deficiency
• ATTP
• AVED
• tocopherol (alpha) transfer protein (ataxia (Friedreich-like) with vitamin Edeficiency)
• tocopherol (alpha) transfer protein
• TPP1
• TTP1

Background

This gene encodes a soluble protein that binds alpha-trocopherol, a form of vitamin E, with high selectivity and affinity. This protein plays an important role in regulating vitamin E levels in the body by transporting vitamin E between membrane vesicles and facilitating the secretion of vitamin E from hepatocytes to circulating lipoproteins. Mutations in this gene cause hereditary vitamin E deficiency (ataxia with vitamin E deficiency, AVED) and retinitis pigmentosa. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TTPA
• Uniprot:
  • P49638(Human)