POT1 Antibody [FITC]

• Reactivity: Hu, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: FITC

POT1 Antibody [FITC] Summary

• Immunogen: A synthetic peptide made to an internal portion of the human POT1 protein sequence (between residues 200-300). [UniProt# Q9NUX5]
• Localization: Nuclear
• Predicted Species: Primate (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This POT1 antibody is use for Western blot, where a band at ~71.4 kDa is seen. Not recommended for ICC/IF. Nitrocellulose membranes may be necessary for optimal results, although this has not been completely determined. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 71.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: monkey (95%), rat (82%), and mouse (72%).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for POT1 Antibody [FITC]

• DKFZp586D211
• HPOT1
• POT1 protection of telomeres 1 homolog
• POT1
• POT1-like telomere end-binding protein
• protection of telomeres 1 homolog (S. pombe)
• protection of telomeres protein 1

Background

POT1 (protection of telomeres protein 1), a member of telombin family, is expressed ubiquitously and localizes to nucleus/chromosomal telomeres. It forms a component of telomerase RNP (ribonucleoprotein) complex which is essential for chromosome termini replication. POT1 participates in shelterin complex/telosome (contains TERF1, TERF2, TINF2, TERF2IP, ACD and POT1 - regulates telomeric length alongwith protecting telomers), telomerase holoenzyme complex (TERT, DKC1, WRAP53/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and TERC) and is also found complexed with TERF1, TINF2 and TNKS1. POT1 interacts with TNKS1 and can heterodimerize with ACD for enhancing telomere elongation via increase in telomerase processivity. POT1 implicates in double-stranded telomeric DNA-binding TRF1 complex which is essential to the regulation of telomere length by cis-inhibition of telomerase. POT1 acts as a single-stranded telomeric DNA-binding protein and thus behaves as a downstream effector of TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. POT1 functional alterations can lead to G-strand overhang loss, chromosomal-end fusions, chromosomal rearrangements, and rapid cell cycle arrest, ultimately leading to senescence and apoptosis. Accordingly, POT1 expression is altered in tumors and is specifically upregulated in therapeutic/radiation-resistant cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POT1