POT1 Antibody [FITC]

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB500-176F
    • Availability
      Product Discontinued

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POT1 Antibody [FITC] Summary

Immunogen
A synthetic peptide made to an internal portion of the human POT1 protein sequence (between residues 200-300). [UniProt# Q9NUX5]
Localization
Nuclear
Predicted Species
Primate (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POT1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10 - 1:500
Application Notes
This POT1 antibody is use for Western blot, where a band at ~71.4 kDa is seen. Not recommended for ICC/IF. Nitrocellulose membranes may be necessary for optimal results, although this has not been completely determined. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
71.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. Immunogen sequence has 95% homology to monkey, 82% homology to rat, and 72% homology to mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for POT1 Antibody [FITC]

  • DKFZp586D211
  • HPOT1
  • POT1 protection of telomeres 1 homolog
  • POT1
  • POT1-like telomere end-binding protein
  • protection of telomeres 1 homolog (S. pombe)
  • protection of telomeres protein 1

Background

POT1 (protection of telomeres protein 1), a member of telombin family, is expressed ubiquitously and localizes to nucleus/chromosomal telomeres. It forms a component of telomerase RNP (ribonucleoprotein) complex which is essential for chromosome termini replication. POT1 participates in shelterin complex/telosome (contains TERF1, TERF2, TINF2, TERF2IP, ACD and POT1 - regulates telomeric length alongwith protecting telomers), telomerase holoenzyme complex (TERT, DKC1, WRAP53/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and TERC) and is also found complexed with TERF1, TINF2 and TNKS1. POT1 interacts with TNKS1 and can heterodimerize with ACD for enhancing telomere elongation via increase in telomerase processivity. POT1 implicates in double-stranded telomeric DNA-binding TRF1 complex which is essential to the regulation of telomere length by cis-inhibition of telomerase. POT1 acts as a single-stranded telomeric DNA-binding protein and thus behaves as a downstream effector of TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. POT1 functional alterations can lead to G-strand overhang loss, chromosomal-end fusions, chromosomal rearrangements, and rapid cell cycle arrest, ultimately leading to senescence and apoptosis. Accordingly, POT1 expression is altered in tumors and is specifically upregulated in therapeutic/radiation-resistant cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Research Areas for POT1 Antibody (NB500-176F)

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Blogs on POT1.

Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.

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Bioinformatics

Gene Symbol POT1
Entrez