Tripeptidyl-Peptidase I/TPP1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Tripeptidyl-Peptidase I/TPP1 Antibody [NBP1-31758] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: Tripeptidyl-Peptidase I/TPP1 Antibody [NBP1-31758] - Mouse liver. TPP1 antibody [N1C1] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tripeptidyl-Peptidase I/TPP1 antibody [N1C1] (NBP1-31758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Bv, Ca, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Tripeptidyl-Peptidase I/TPP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Tripeptidyl-Peptidase I/TPP1 Antibody - BSA Free (NBP1-31758) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Tripeptidyl-Peptidase I/TPP1. The exact sequence is proprietary.
• Localization: Lysosome, Melanosome
• Predicted Species: Bovine (91%), Chimpanzee (100%), Canine (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TPP1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Tripeptidyl-Peptidase I/TPP1 Antibody - BSA Free

• Cell growth-inhibiting gene 1 protein
• ceroid-lipofuscinosis, neuronal 2, late infantile (Jansky-Bielschowsky disease)
• CLN2
• CLN2EC 3.4.14.9
• growth-inhibiting protein 1
• LINCL
• LPIC
• lysosomal pepstatin insensitive protease
• Lysosomal pepstatin-insensitive protease
• MGC21297
• TPP1
• TPP-1
• TPP-I
• Tripeptidyl aminopeptidase
• tripeptidyl peptidase I
• tripeptidyl-peptidase 1
• TripeptidylPeptidase I
• Tripeptidyl-Peptidase I

Background

This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TPP1