WRN Antibody

Western Blot: WRN Antibody [NB100-471] - Human colon cancer cells with siRNA Control, siRNA pool of siWrn and 3 different sets of siRNA Wrn. GAPDH laoding control. Western blot image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: WRN Antibody [NB100-471] - Analysis of WRN in untreated, asynchronous HeLa cells using anti-WRN antibody. The primary antibody was used at a dilution of 1:100, incubated for 1 hour at room temperature in 1XPBS. Image from verified customer review.

Western Blot: WRN Antibody [NB100-471] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells.

Western Blot: WRN Antibody [NB100-471] - Detection of WRN in 293T whole cell lysate using NB 100-471.

Western Blot: WRN Antibody [NB100-471] - Detection of human WRN on HeLa whole cell lysate using NB100-471. WRN was immunoprecipitated with NB100-471 or rabbit anti-WRN antibody NB100-472.

Immunoprecipitation: WRN Antibody [NB100-471] - Detection of human WRN by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WRN antibody NB100-471 used for WB at 0.2 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. WRN was also immunoprecipitated by rabbit anti-WRN antibody NB100-472, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

WRN Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 400 and 450 of human Werner Syndrome Helicase using the numbering given in SwissProt entry Q14191 (GeneID 7486).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WRN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Simple Western reported by internal validation
  • Western Blot 1:5000 - 1:25000
• Application Notes: Use in ICC/IF reported in scientific literature (PMID 25294835). Use in chromatin immunoprecipitation reported in scientific literature (PMID: 20065033). Use in Immunohistochemistry reported in scientific literature (PMID: 30146558). WRN antibody validated for WB from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for WRN Antibody

• DKFZp686C2056
• DNA helicase, RecQ-like type 3
• EC 3.1
• EC 3.1.-.-
• EC 3.6.1
• EC 3.6.4.12
• Exonuclease WRN
• RecQ protein-like 2
• RecQ3
• RECQ3RECQL3
• RECQL2
• RECQL2Werner syndrome ATP-dependent helicase
• Werner syndrome
• Werner syndrome, RecQ helicase-like
• WRN

Background

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner Syndrome, an autosomal recessive disorder characterized by premature aging.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WRN Antibody (NB100-471)(12)

Publications using NB100-471

• Datta A, Biswas K, Sommers JA Et al. WRN helicase safeguards deprotected replication forks in BRCA2-mutated cancer cells Nature communications 2021-11-12 [PMID: 34772932] (ICC/IF)
• Thangavel S, Berti M, Levikova M et al. DNA2 drives processing and restart of reversed replication forks in human cells. J Cell Biol 2015-03-02 [PMID: 25733713] (Human)
• Maddukuri L, Ketkar A, Eddy S et al. The Werner syndrome protein limits the error-prone 8-oxo-dG lesion bypass activity of human DnA polymerase kappa. Nucleic Acids Res. 2014-10-07 [PMID: 25294835] (ICC/IF, Human)
• Betous R, Glick GG, Zhao R, Cortez D. Identification and Characterization of SMARCAL1 Protein Complexes. PLoS One 2013-05-09 [PMID: 23671665] (WB, Human)
• Maddukuri L, Ketkar A, Eddy S et al. Enhancement of human DNA polymerase Eta activity and fidelity is dependent upon a bipartite interaction with the Werner syndrome protein. J Biol Chem. 2012-12-07 [PMID: 23045531] (WB)
• Thangavel S, Mendoza-Maldonado R, Tissino E et al. Human RECQ1 and RECQ4 helicases play distinct roles in DNA replication initiation. Mol Cell Biol 2010-03-01 [PMID: 20065033] (Chemotaxis, Human)
• Rizzo A, Salvati E, Porru M et al. Stabilization of quadruplex DNA perturbs telomere replication leading to the activation of an ATR-dependent ATM signaling pathway. Nucleic Acids Res 2009-09-01 [PMID: 19596811]
• Harrigan JA, Piotrowski J, Di Noto L et al. Metal-catalyzed Oxidation of the Werner Syndrome Protein Causes Loss of Catalytic Activities Impaired Protein-Protein Interactions. J Biol Chem, 2007;282(50):36403-11. 2007-12-14 [PMID: 17911100]
• Das A, Boldogh I, Lee JW et al. The human Werner syndrome protein stimulates repair of oxidative DNA base damage by the DNA glycosylase NEIL1. J Biol Chem 2007-09-01 [PMID: 17611195]
• Chen H, Wu X, Wang X et al. Downregulation of RECQL4 inhibits gastric cancer cell proliferation and induces cell cycle arrest at G0/G1 phase. Veterinary Microbiology. (WB, Human)
• Hayashi K, Tasaka T, Kondo T et al. Successful Cord Blood Transplantation in a Werner Syndrome Patient with High-risk Myelodysplastic Syndrome Intern. Med. 2018-08-24 [PMID: 30146558] (IF/IHC, Human)
• Nyunoya T, Monick MM, Klingelhutz AL et al. Cigarette Smoke Induces Cellular Senescence via Werner's Syndrome Protein Downregulation. Am J Respir Crit Care Med :200802-200320OC. 2008-01-01 [PMID: 19011155]

Reviews for WRN Antibody (NB100-471) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer WB Human 12/09/2019

Summary

• Application: Western Blot
• Sample Tested: Colon cancer cell line
• Species: Human
• Lot: A2

Comments

• Comments: Ab used at 1/1000_ON

reviewed by: Robert Eoff IF Human 01/12/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Asynchronous HeLa cells
• Species: Human
• Lot: A2

Bioinformatics

• Gene Symbol: WRN
• Entrez:
  • 7486(Human)
• Uniprot:
  • Q14191(Human)