WRN Antibody

Images

 
Western Blot: WRN Antibody [NB100-471] - Detection of human WRN on HeLa whole cell lysate using NB100-471. WRN was immunoprecipitated with NB100-471 or rabbit anti-WRN antibody NB100-472.
Immunocytochemistry/ Immunofluorescence: WRN Antibody [NB100-471] - Analysis of WRN in untreated, asynchronous HeLa cells using anti-WRN antibody. The primary antibody was used at a dilution of 1:100, incubated for 1 ...read more
Western Blot: WRN Antibody [NB100-471] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells.
Western Blot: WRN Antibody [NB100-471] - Detection of WRN in 293T whole cell lysate using NB 100-471.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

       

WRN Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 400 and 450 of human Werner Syndrome Helicase using the numbering given in SwissProt entry Q14191 (GeneID 7486).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WRN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:25000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25294835)
Reviewed Applications
Read 1 Review rated 4
using
NB100-471 in the following applications:

Publications
Read Publications using
NB100-471 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for WRN Antibody

  • DKFZp686C2056
  • DNA helicase, RecQ-like type 3
  • EC 3.1
  • EC 3.6.1
  • EC 3.6.4.12
  • Exonuclease WRN
  • RecQ protein-like 2
  • RecQ3
  • RECQ3RECQL3
  • RECQL2Werner syndrome ATP-dependent helicase
  • Werner syndrome
  • Werner syndrome, RecQ helicase-like

Background

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner Syndrome, an autosomal recessive disorder characterized by premature aging.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-471
Species: Hu
Applications: WB, ChIP, ICC/IF, IP

Publications for WRN Antibody (NB100-471)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ChIP, ICC/IF, WB.


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ChIP
(1)
ICC/IF
(1)
WB
(2)
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Human
(4)
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Showing Publications 1 - 9 of 9.
Publications using NB100-471 Applications Species
Thangavel S, Berti M, Levikova M et al. DNA2 drives processing and restart of reversed replication forks in human cells. J Cell Biol 2015 Mar 2 [PMID: 25733713] (Human) Human
Maddukuri L, Ketkar A, Eddy S et al. The Werner syndrome protein limits the error-prone 8-oxo-dG lesion bypass activity of human DnA polymerase kappa. Nucleic Acids Res. 2014 Oct 07 [PMID: 25294835] (ICC/IF, Human) ICC/IF Human
Betous R, Glick GG, Zhao R, Cortez D. Identification and Characterization of SMARCAL1 Protein Complexes. PLoS One 2013 May 9 [PMID: 23671665] (WB, Human) WB Human
Maddukuri L, Ketkar A, Eddy S et al. Enhancement of human DNA polymerase Eta activity and fidelity is dependent upon a bipartite interaction with the Werner syndrome protein. J Biol Chem. 2012 Dec 7 [PMID: 23045531] (WB) WB
Thangavel S, Mendoza-Maldonado R, Tissino E et al. Human RECQ1 and RECQ4 helicases play distinct roles in DNA replication initiation. Mol Cell Biol 2010 Mar [PMID: 20065033] (ChIP, Human) ChIP Human
Rizzo A, Salvati E, Porru M et al. Stabilization of quadruplex DNA perturbs telomere replication leading to the activation of an ATR-dependent ATM signaling pathway. Nucleic Acids Res 2009 Sep [PMID: 19596811]
Nyunoya T, Monick MM, Klingelhutz AL et al. Cigarette Smoke Induces Cellular Senescence via Werner's Syndrome Protein Downregulation. Am J Respir Crit Care Med :200802-200320OC. 2008 [PMID: 19011155]
Harrigan JA, Piotrowski J, Di Noto L et al. Metal-catalyzed Oxidation of the Werner Syndrome Protein Causes Loss of Catalytic Activities Impaired Protein-Protein Interactions. J Biol Chem, 2007;282(50):36403-11. 2007 December 14. [PMID: 17911100]
Das A, Boldogh I, Lee JW et al. The human Werner syndrome protein stimulates repair of oxidative DNA base damage by the DNA glycosylase NEIL1. J Biol Chem 2007 Sep [PMID: 17611195]

Review for WRN Antibody (NB100-471) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using NB100-471:
Filter by Applications
IF
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence WRN NB100-471
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reviewed by:
Anonymous
IF Human 01/12/2015
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Summary

ApplicationImmunofluorescence
Sample TestedAsynchronous HeLa cells
SpeciesHuman
LotA2

Blocking

Blocking Details1X PBS with 5 mg/mL BSA, 30 minutes RT

Primary Anitbody

Dilution Ratio1 to 100 dilution, 1 hour at room temperature in 1X PBS

Secondary Antibody

Secondary DescriptionDyLight conjugated goat anti-rabbit IgG
Secondary Manufacturer Cat#SA5-10033
Secondary Concentration1 to 100 dilution in 1X PBS

Details

Detection NotesOlympus IX81 fluorescence microscope
Fixation DetailsFixed-3.7% (v/v) formaldehyde, 20 min at room temperature (RT), permeabilized-0.2% (v/v) Triton X-100 in 1X PBS, 30 min RT
Wash Description1X PBS, 5 minutes, 3 times

Comments

CommentsAfter washing with 1X PBS, coverslips were mounted on Vectashield (Vector Laboratories).

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for WRN Antibody (NB100-471) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional WRN Products

Bioinformatics Tool for WRN Antibody (NB100-471)

Discover related pathways, diseases and genes to WRN Antibody (NB100-471). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for WRN Antibody (NB100-471)

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Pathways for WRN Antibody (NB100-471)

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PTMs for WRN Antibody (NB100-471)

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Research Areas for WRN Antibody (NB100-471)

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Blogs on WRN.

Not as Pluripotent as You Used to Be: Embryonic Stem Cell Markers and the Aging Process
We at Novus Biologicals recently extended our antibody catalog to include several embryonic stem cells (ESC) antibodies validated for use in FACS (fluorescent activated cell sorting) assays. Among them was Oct4, which recently became the focus of an i...  Read full blog post.

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
01/12/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol WRN
Entrez
Uniprot