POT1 Antibody [DyLight 488]

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Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 488

POT1 Antibody [DyLight 488] Summary

Immunogen
A synthetic peptide made to an internal portion of the human POT1 protein sequence (between residues 200-300). [UniProt# Q9NUX5]
Localization
Nuclear
Predicted Species
Primate (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This POT1 antibody is use for Western blot, where a band at ~71.4 kDa is seen. Not recommended for ICC/IF. Nitrocellulose membranes may be necessary for optimal results, although this has not been completely determined.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
71.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. Immunogen sequence has 95% homology to monkey, 82% homology to rat, and 72% homology to mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for POT1 Antibody [DyLight 488]

  • DKFZp586D211
  • HPOT1
  • POT1 protection of telomeres 1 homolog
  • POT1
  • POT1-like telomere end-binding protein
  • protection of telomeres 1 homolog (S. pombe)
  • protection of telomeres protein 1

Background

Telomere maintenance is essential for protection against changes in telomere length, which has been implicated in ageing and certain cancers. POT1 (protection of telomeres 1), a telomere regulator, is a single-stranded telomeric DNA-binding protein that controls telomerase-mediated telomere elongation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for POT1 Antibody (NB500-176G) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB500-176B
DyLight 550 Labeled NB500-176R
DyLight 650 Labeled NB500-176C
HRP Labeled NB500-176H

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Bioinformatics Tool for POT1 Antibody (NB500-176G)

Discover related pathways, diseases and genes to POT1 Antibody (NB500-176G). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for POT1 Antibody (NB500-176G)

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Blogs on POT1.

Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.

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Bioinformatics

Gene Symbol POT1
Entrez