PGC1 alpha Antibody

Images

 
Western Blot: PGC1 alpha Antibody [NB100-60955] - (0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PGC1 alpha Antibody Summary

Immunogen
Peptide with sequence C-DGLFDDSEDESDK corresponding to internal region according to NP_037393.1.
Epitope
DGLFDDSEDESDK
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PPARGC1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3ug/ml
  • Peptide ELISA detection limit 1:8000
Application Notes
WB: Approx. 110 kDa band observed in human skeletal muscle lysates (calculated MW of 91.0 kDa band according to NP_037393.1). IHC-P usage is reported in scientifc literature (PMID: 23763823).
Theoretical MW
91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
PGC1 alpha Lysate (NBL1-14636)
Reviewed Applications
Read 1 Review rated 4
using
NB100-60955 in the following applications:

Publications
Read Publications using
NB100-60955 in the following applications:

  • IHC
    1 publication
  • 1 publication
  • WB
    1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PGC1 alpha Antibody

  • LEM6
  • Ligand effect modulator 6
  • ligand effect modulator-6
  • peroxisome proliferative activated receptor, gamma, coactivator 1, alpha
  • peroxisome proliferator-activated receptor gamma coactivator 1-alpha
  • peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
  • PGC1 alpha
  • PGC1A
  • PGC-1-alpha
  • PGC1APGC-1(alpha)
  • PGC1peroxisome proliferative activated receptor, gamma, coactivator 1
  • PPAR gamma coactivator variant form
  • PPAR gamma coactivator-1
  • PPAR-gamma coactivator 1-alpha
  • PPARGC1A
  • PPARGC-1-alpha
  • PPARGC1PGC-1v

Background

The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-60955
Species: Hu
Applications: WB, PEP-ELISA

Publications for PGC1 alpha Antibody (NB100-60955)(5)

Review for PGC1 alpha Antibody (NB100-60955) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-60955:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot PGC1 alpha NB100-60955
Enlarge
reviewed by:
Hongxia Hu
WB Human 01/09/2011
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Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate, Sample Amount: 30ug
SpeciesHuman
CommentsExamined only MW>72kDa. Two bands are observed. About 130kDa and 105kDa.

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in TBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:1000, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: bovine anti goat HRP, Secondary Ab Dilution Ratio: 1:2500

Details

Detection NotesDetection Method: ECL Plus, Exposure Time: 5 minutes, Specific Bands: 105 kDa, Non-Specific Bands: 130 kDa

Comments

CommentsExamined only MW>72kDa. Two bands are observed. About 130kDa and 105kDa.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PGC1 alpha Antibody (NB100-60955). (Showing 1 - 2 of 2 FAQs).

  1. Please differentiate me between PPAR and PGC clearly.i am confuse to know the differ these two. waiting for your reply
    • Thank you very much for contacting Novus Biologicals technical support team and sharing your query on the differences between PGC-1 alpha and PPAR. These are two different proteins encoded by their respective genes and serves different functions. PGC-1 alpha (PGC1A or PPAR gamma coactivator 1-alpha) is a transcriptional co-activator for steroid receptors and nuclear receptors, and it regulates diverse aspects of cellular physiology. It up-regulates the transcriptional activity of PPAR-gamma /thyroid hormone receptor on the uncoupling protein promoter; regulates the key mitochondrial genes involved in adaptive thermogenesis; implicates in the metabolic reprogramming in response to nutrients availability through the coordination of the expression of a wide array of genes involved in the regulation of glucose and fatty acid metabolism. Among our PGC-1 alpha antibodies, NBP1-04676 is our best selling product with nice customer feedback and citations in at least 13 research publications. PPAR (PPAR alpha) on the other hand is a ligand-activated transcription factor which gets activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine, and oleylethanolamide (a naturally occurring lipid that regulates satiety), and acts as a key regulator of lipid metabolism. It also acts as a receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. It regulates the peroxisomal beta-oxidation pathway of fatty acids, and also functions as transcription activator for the ACOX1 and P450 genes. We have a variety of PPAR alpha antibodies. I hope you will find this information helpful but please let me know if I can support you with anything else from my end. Thank you very much for choosing Novus Biologicals as your quality reagent supplier and we wish you the best with your research projects.
  2. My question is whether the antibody (anti-PGC) detects the phosphorylated PGC protein? Or if it just detects the total PGC protein?
    • NB100-60955 is not specific for a particular phosphorylated form of PGC-1 alpha. This protein is only phosphorylated on two residues but has 13 known acetylation sites, which is why the protein is detected at around 100 kDa instead of the predicted 91 kDa. This antibody should detect the unphosphorylated and unacetylated form as well, if they are present in your sample.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Hongxia Hu
01/09/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol PPARGC1A
Entrez
Uniprot