Perilipin-2/ADFP Antibody - BSA Free

Images

 
Orthogonal Strategies: Formalin-fixed paraffin-embedded tissue sections of mouse intestine were probed for Perilipin-2/ADFP mRNA (ACD RNAScope Probe, catalog #577111; Fast Red chromogen, ACD catalog # 322750). ...read more
Simple Western lane view shows a specific band for ADFP in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Biological Strategies: Oleic acid increasesI2 oxidation, glucose utilization and expression of genes involved in LCFA metabolism in U138 GBM cells. Cropped western blot images and quantitation of PLIN2 and GAPDH ...read more
ADFP antibody was tested in mouse liver using DAB with hematoxylin counterstain.
NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Perilipin-2/ADFP Antibody NB110-40877 at 1 ug/ml overnight ...read more
Detection of murine ADFP in mouse liver lysate.
HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Perilipin-2/ADFP at a 1:100 dilution overnight at 4C and ...read more
HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Perilipin-2/ADFP Antibody NB110-40877 at 1 ug/ml overnight at ...read more
Lack of MT4-MMP in patrolling monocytes leads to the accumulation of Mafb+AIM+ macrophages in incipient atherosclerotic plaques. c) Representative images of transverse sections of aortic sinus from BM-transplanted ...read more
Manipulation of LDLR or LPC abundance could reprogramme LD metabolism and increase DNA adduct formation for enhancing the cisplatin cytotoxic effects. H): Perilipin-2, which is an LD marker protein, was detected in OE ...read more
Oleic acid increases beta oxidation, glucose utilization and expression of genes involved in LCFA metabolism in U138 GBM cells. (B) Cropped western blot images and quantitation of PLIN2 and GAPDH protein levels from ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, Dual ISH-IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Perilipin-2/ADFP Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to a C-terminal region of mouse ADFP (within residues 350-425) [Swiss-Prot# P43883]
Localization
Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLIN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 35041644)
  • Immunohistochemistry-Paraffin 1:200
  • Simple Western 1:50
  • Western Blot 2 ug/ml
Application Notes
This ADFP antibody can be used for Western blot and Immunofluorescence/Immunocytochemistry. In Western blot a band is observed at approx. 51 kDa. In ICC/IF, membrane staining was observed in HeLa cells. In IHC-P, staining was observed on the membrane of mouse liver cells Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Adrenal Whole Tissue Lysate (Adult Whole Liver Cirrhosis)
Human Liver Whole Tissue Lysate (Adult Whole Normal)
Mouse Liver Whole Tissue Lysate (Adult Whole Normal)
Perilipin-2/ADFP Overexpression Lysate
Control Peptide
Perilipin-2/ADFP Protein (NB110-40877PEP)
Reviewed Applications
Read 1 Review rated 5
using
NB110-40877 in the following applications:

Publications
Read Publications using
NB110-40877 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 29053516). Use in Bovine reported in scientific literature (PMID:32647143).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Perilipin-2/ADFP Antibody - BSA Free

  • ADFP
  • Adipophilin
  • Adipose differentiation-related proteinadipophilin
  • ADRP
  • MGC10598
  • perilipin 2
  • Perilipin2
  • Perilipin-2
  • PLIN2

Background

Perilipin-2/Plin-2, also known as adipose differentiation-related protein (ADFP), ADRP, and adipophilin, is a member of the perilipin family of lipid droplet (LD) proteins that functions in lipid accumulation, lipid storage, and LD formation (1,2). Perilipin-2 is ubiquitously expressed and is the major LD protein of the liver (1-3). Human perilipin-2 is 437 amino acids (aa) in length with a theoretical molecular weight of 48 kDa (1,4). Human and mouse perilipin-2 share 81.8% sequence identity. One defining structural feature of perilipin-2 and other LD-related proteins is formation of amphipathic alpha helices (1,3,5). The perilipin-2 protein contains a PAT (perilipin A, ADRP, TIP47) domain, a 11-mer repeat motif, and a 4-helix bundle (1,5). The PAT domain has a role in triglyceride (TG) stabilization, the 11-mer repeat motif functions in cytoplasmic LD binding, and the 4-helix bundle facilitates membrane binding (5). Unlike LDs coated with other perilipin family members (e.g., perilipin-1 and perilipin-5), LDs coated with perilipin-2 are more permissive to lipolysis and protective of lipids and TGs (1-3). Studies have revealed that perilipin-2 knockout (KO) in mice is associated with resistance to fatty liver disease and diet-induced obesity (1). On the other hand, overexpression of perilipin-2 causes increased lipid and TG accumulation, which is a feature of many metabolic, cardiovascular, and age-related diseases (2). Additional research has found higher levels of perilipin-2 is associated with certain cancers such as colorectal cancer and renal carcinomas, and it may be a useful biomarker for detection (2). Though more research is needed, it is possible that inhibition or blocking of perilipin-2 may be a key strategy to combatting several pathologies (2).

References

1. Itabe H, Yamaguchi T, Nimura S, Sasabe N. Perilipins: a diversity of intracellular lipid droplet proteins. Lipids Health Dis. 2017;16(1):83. https://doi.org/10.1186/s12944-017-0473-y

2. Conte M, Franceschi C, Sandri M, Salvioli S. Perilipin 2 and Age-Related Metabolic Diseases: A New Perspective. Trends Endocrinol Metab. 2016;27(12):893-903. https://doi.org/10.1016/j.tem.2016.09.001

3. Sztalryd C, Brasaemle DL. The perilipin family of lipid droplet proteins: Gatekeepers of intracellular lipolysis. Biochim Biophys Acta Mol Cell Biol Lipids. 2017;1862(10 Pt B):1221-1232. https://doi.org/10.1016/j.bbalip.2017.07.009

4. Uniprot (Q99541)

5. Chong BM, Reigan P, Mayle-Combs KD, Orlicky DJ, McManaman JL. Determinants of adipophilin function in milk lipid formation and secretion. Trends Endocrinol Metab. 2011;22(6):211-217. https://10.1016/j.tem.2011.04.003

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Perilipin-2/ADFP Antibody (NB110-40877)(60)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Plant.

We have publications tested in 7 applications: ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, SDS-Page, WB.


Filter By Application
ICC/IF
(4)
IF/IHC
(10)
IHC
(1)
IHC-Fr
(3)
IHC-P
(5)
SDS-Page
(1)
WB
(22)
All Applications
Filter By Species
Human
(12)
Mouse
(31)
Rat
(2)
Bovine
(1)
Plant
(1)
All Species
Showing Publications 1 - 10 of 60. Show All 60 Publications.
Publications using NB110-40877 Applications Species
Renier TJ, Paetz OR, Paal MC et al. Changing the phospholipid composition of lipid droplets alters localization of select lipid droplet proteins microPublication biology 2023-11-03 [PMID: 38021172] (SDS-Page, Mouse) SDS-Page Mouse
Kothari V, Savard C, Tang J et al. sTREM2 is a plasma biomarker for human NASH and promotes hepatocyte lipid accumulation Hepatology communications 2023-11-01 [PMID: 37820278] (ICC/IF, Mouse) ICC/IF Mouse
Wieland EB, Kempen LJAP, Lu C et al. Protocol for multispectral imaging on cryosections to map myeloid cell heterogeneity in its spatial context STAR protocols 2023-09-23 [PMID: 37742177] (IHC-Fr)

Details:
Alexa Fluor 488 conjugation used. Diltion 1:29
IHC-Fr
Ibayashi M, Aizawa R, Mitsui J, Tsukamoto S Lipid droplet synthesis is associated with angiogenesis in mouse ovarian follicles Biology of reproduction 2022-12-28 [PMID: 36579469]
Yu S, Wang L, Che D Et al. Targeting CRABP-II Overcomes Pancreatic Cancer Drug Resistance By Reversing Lipid Raft Cholesterol Accumulation and AKT Survival Signaling Research Square Sep 30 2021 (IHC-P, Human) IHC-P Human
Choi YJ, Yun SH, Yu J et al. Chaperone-mediated autophagy dysregulation during aging impairs hepatic fatty acid oxidation via accumulation of NCoR1 Molecular metabolism 2023-07-29 [PMID: 37524243] (WB, Mouse) WB Mouse
Androvic P, Schifferer M, Perez Anderson K et al. Spatial Transcriptomics-correlated Electron Microscopy maps transcriptional and ultrastructural responses to brain injury Nature communications 2023-07-11 [PMID: 37433806] (IHC, Mouse) IHC Mouse
Pratelli G, Di Liberto D, Carlisi D et al. Hypertrophy and ER Stress Induced by Palmitate Are Counteracted by Mango Peel and Seed Extracts in 3T3-L1 Adipocytes International journal of molecular sciences 2023-03-12 [PMID: 36982490] (WB, Plant) WB Plant
Mi?i? B, Djordjevic A, Veli?kovi? N et al. AMPK Activation as a Protective Mechanism to Restrain Oxidative Stress in the Insulin-Resistant State in Skeletal Muscle of Rat Model of PCOS Subjected to Postnatal Overfeeding Biomedicines 2023-05-30 [PMID: 37371678]
Show All 60 Publications.

Review for Perilipin-2/ADFP Antibody (NB110-40877) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB110-40877:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Perilipin-2/ADFP NB110-40877
Enlarge
5
reviewed by:
Natasa Velickovic
WB Rat 06/16/2020
View

Summary

ApplicationWestern Blot
Sample TestedLiver whole cell extract,Liver whole tissue homogenate, Sample Amount: 50ug
SpeciesRat
LotF-3

Comments

CommentsConcentration 1:1000, over night
Secondary antibody: 1: 20000

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Perilipin-2/ADFP Antibody (NB110-40877). (Showing 1 - 2 of 2 FAQs).

  1. I would appreciate if you are able to provide the exact amino acid sequence of the immunogens. The customer would like to check the percentage alignment with the chicken protein to determine which of the antiodies woudl be better to try in this species.
    • I am unable to provide you with this information however I can tell you that there is a 71% homology between the chicken protein and human protein. Our lab only recommends use if the homology is 85% or above.
  2. I went trought your product datasheet, but I didn't find any information about the dilution I have to use for the western blot and if it's in 5% milk-TBST or other blocking buffer. Could you please provide me those information?
    • For our product NB110-40877, we recommend a starting dilution of 2 ug/ml for western blot. Our recommended diluent buffer is 5% nonfat milk in TBST.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Perilipin-2/ADFP Products

Research Areas for Perilipin-2/ADFP Antibody (NB110-40877)

Find related products by research area.

Blogs on Perilipin-2/ADFP.

The affects of Perilipin 2 on diet and metabolism
Perilipin 2 belongs to the Perilipin family, which consists of proteins that coat intracellular lipid storage droplets. Perilipin 2 in particular is involved in lipid globule surface membrane composition, and has also been implicated in the develo...  Read full blog post.

mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Natasa Velickovic
06/16/2020
Application: WB
Species: Rat

Bioinformatics

Gene Symbol PLIN2
Entrez
Uniprot