Perilipin-2/ADFP Antibody

Images

 
Western Blot: Perilipin-2/ADFP Antibody [NB110-40877] - Detection of murine ADFP in mouse liver lysate.
Immunocytochemistry/ Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more
Immunohistochemistry: Perilipin-2/ADFP Antibody [NB110-40877] - ADFP antibody was tested in mouse liver using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - Detection of ADFP (Green) in Hela cells using NB110-40877 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.
Simple Western: Perilipin-2/ADFP Antibody [NB110-40877] - Simple Western lane view shows a specific band for ADFP in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Perilipin-2/ADFP Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal region of mouse ADFP (within residues 350-425) [Swiss-Prot# P43883]
Localization
Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLIN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This ADFP antibody can be used for Western blot and Immunofluorescence/Immunocytochemistry. In Western blot a band is observed at approx. 51 kDa. In ICC/IF, membrane staining was observed in HeLa cells. In IHC-P, staining was observed on the membrane of mouse liver cells Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Adrenal Whole Tissue Lysate (Adult Whole Liver Cirrhosis)
Human Liver Whole Tissue Lysate (Adult Whole Normal)
Mouse Liver Whole Tissue Lysate (Adult Whole Normal)
Perilipin-2/ADFP Overexpression Lysate
Control Peptide
Publications
Read Publications using
NB110-40877 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 29053516).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Perilipin-2/ADFP Antibody

  • ADFP
  • Adipophilin
  • Adipose differentiation-related proteinadipophilin
  • ADRP
  • MGC10598
  • perilipin 2
  • Perilipin2
  • Perilipin-2
  • PLIN2

Background

ADFP (aka adipophilin) can be found on the surface of lipid droplets in most mammalian cell types. ADFP is a major protein component of lipid storage droplets. Lipid droplet metabolism is likely controlled by the coat proteins of the perilipin family, which include ADFP, perilipin, TIP47, S3-12, and PAT-1. Perilipin family proteins localize to lipid droplets, either constitutively (perilipin, ADFP) or in response to lipogenic and/or lipolytic stimuli (TIP47, S3-12, and PAT-1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB110-40764
Species: Hu, Mu, Po, Pm
Applications: WB, ICC/IF
NB110-40760
Species: Hu, Mu, Rt, Po, Fi
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-22106
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
NB100-1269
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, PEP-ELISA
44110002
Species: Hu
Applications: WB, ELISA, ICC/IF
NB300-537
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP
NB110-60509
Species: Hu, Mu, Po, Bv
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
AF5365
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-47602
Species: Hu, Mu, Bv, Vb
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP2-13776
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB110-37253
Species: Hu, Mu, Rt
Applications: WB
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
NB400-114
Species: Hu, Mu, Rt, Po, Ch, Fe, Ha, Pl, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB400-144
Species: Hu, Mu, Rt, Po, Bv, Pm, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB600-582
Species: Hu, Mu, Rt, Ca, Ch, SyHa, Ha, Mk
Applications: WB, Simple Western, IHC

Publications for Perilipin-2/ADFP Antibody (NB110-40877)(20)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC
(3)
IHC-P
(2)
WB
(9)
All Applications
Filter By Species
Human
(6)
Mouse
(11)
Rat
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NB110-40877 Applications Species
Farmer BC, Kluemper J, Johnson LA. Apolipoprotein E4 Alters Astrocyte Fatty Acid Metabolism and Lipid Droplet Formation Cells Feb 20 2019 [PMID: 30791549] (WB) WB
Larsen S, Vigelso A, Dandanell S et al. Simvastatin-Induced Insulin Resistance May Be Linked to Decreased Lipid Uptake and Lipid Synthesis in Human Skeletal Muscle: the LIFESTAT Study. Journal of Diabetes Research Sep 12 2018 (WB, Human) WB Human
Asimakopoulou A, Vucur M, Luedde T et al. Perilipin 5 and Lipocalin 2 Expression in Hepatocellular Carcinoma Cancers (Basel) Mar 19 2019 [PMID: 30893876] (WB, Human, Mouse) WB Human, Mouse
Chali F, Milior G, Marty S et al. Lipid markers and related transcripts during excitotoxic neurodegeneration in kainate-treated mice Eur. J. Neurosci. Feb 14 2019 [PMID: 30767299] (IHC, Mouse) IHC Mouse
Zhang P, Csaki LS, Ronquillo E et al. Lipin 2/3 phosphatidic acid phosphatases maintain phospholipid homeostasis to regulate chylomicron synthesis J. Clin. Invest. Jan 2 2019 [PMID: 30507612] (WB, Human) WB Human
Larsen Steen, Vigelso Andreas, Dandanell Sune et al. Simvastatin-Induced Insulin Resistance May Be Linked to Decreased Lipid Uptake and Lipid Synthesis in Human Skeletal Muscle: the LIFESTAT Study. J Diabetes Res.  2018 Sep 12 [PMID: 30276217] (WB, Human) WB Human
Li Z, Rasmussen ML, Li J et al. Periodized low protein-high carbohydrate diet confers potent, but transient, metabolic improvements. Mol Metab Aug 28 2018 [PMID: 30193785] (WB, Mouse) WB Mouse
Madushani Herath KHIN, Cho J, Kim A et al. Phenolic acid and flavonoid-rich fraction of Sasa quelpaertensis Nakai leaves prevent alcohol induced fatty liver through AMPK activation J Ethnopharmacol. [PMID: 29906537] (IHC, Mouse) IHC Mouse
Blossom SJ, Fernandes L, Bai S et al. Opposing actions of developmental trichloroethylene and high-fat-diet co-exposure on markers of lipogenesis and inflammation in autoimmune-prone mice. Toxicol. Sci. Apr 12 2018 [PMID: 29669109] (IHC, Mouse) IHC Mouse
Clemente C, Rius C, Alonso-Herranz L et al. MT4-MMP deficiency increases patrolling monocyte recruitment to early lesions and accelerates atherosclerosis. Nat Commun. Mar 2 2018 [PMID: 29500407] (IHC-P, Mouse) IHC-P Mouse
Show All 20 Publications.

Reviews for Perilipin-2/ADFP Antibody (NB110-40877) (0)

There are no reviews for Perilipin-2/ADFP Antibody (NB110-40877). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Perilipin-2/ADFP Antibody (NB110-40877). (Showing 1 - 2 of 2 FAQs).

  1. I would appreciate if you are able to provide the exact amino acid sequence of the immunogens. The customer would like to check the percentage alignment with the chicken protein to determine which of the antiodies woudl be better to try in this species.
    • I am unable to provide you with this information however I can tell you that there is a 71% homology between the chicken protein and human protein. Our lab only recommends use if the homology is 85% or above.
  2. I went trought your product datasheet, but I didn't find any information about the dilution I have to use for the western blot and if it's in 5% milk-TBST or other blocking buffer. Could you please provide me those information?
    • For our product NB110-40877, we recommend a starting dilution of 2 ug/ml for western blot. Our recommended diluent buffer is 5% nonfat milk in TBST.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-40877AF350
Alexa Fluor 405 NB110-40877AF405
Alexa Fluor 488 NB110-40877AF488
Alexa Fluor 532 NB110-40877AF532
Alexa Fluor 594 NB110-40877AF594
Alexa Fluor 647 NB110-40877AF647
Alexa Fluor 700 NB110-40877AF700
Alexa Fluor 750 NB110-40877AF750
Biotin NB110-40877B
DyLight 350 NB110-40877UV
DyLight 405 NB110-40877V
DyLight 488 NB110-40877G
DyLight 550 NB110-40877R
DyLight 594 NB110-40877DL594
DyLight 650 NB110-40877C
DyLight 680 NB110-40877FR
DyLight 755 NB110-40877IR
FITC NB110-40877F
HRP NB110-40877H
Janelia Fluor 549 NB110-40877JF549
Janelia Fluor 646 NB110-40877JF646

Additional Perilipin-2/ADFP Products

Bioinformatics Tool for Perilipin-2/ADFP Antibody (NB110-40877)

Discover related pathways, diseases and genes to Perilipin-2/ADFP Antibody (NB110-40877). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Perilipin-2/ADFP Antibody (NB110-40877)

Discover more about diseases related to Perilipin-2/ADFP Antibody (NB110-40877).
 

Pathways for Perilipin-2/ADFP Antibody (NB110-40877)

View related products by pathway.

PTMs for Perilipin-2/ADFP Antibody (NB110-40877)

Learn more about PTMs related to Perilipin-2/ADFP Antibody (NB110-40877).
 

Research Areas for Perilipin-2/ADFP Antibody (NB110-40877)

Find related products by research area.

Blogs on Perilipin-2/ADFP.

The affects of Perilipin 2 on diet and metabolism
Perilipin 2 belongs to the Perilipin family, which consists of proteins that coat intracellular lipid storage droplets. Perilipin 2 in particular is involved in lipid globule surface membrane composition, and has also been implicated in the develo...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Perilipin-2/ADFP Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol PLIN2
Entrez
Uniprot