Perilipin-2/ADFP Antibody

Images

 
Western Blot: Perilipin-2/ADFP Antibody [NB110-40877] - Detection of murine ADFP in mouse liver lysate.
Immunocytochemistry/ Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more
Immunocytochemistry/ Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - Detection of ADFP (Green) in Hela cells using NB110-40877 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.
Immunocytochemistry/ Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - ADFP antibody was tested in mouse liver using DAB with hematoxylin counterstain.
Simple Western: Perilipin-2/ADFP Antibody [NB110-40877] - Simple Western lane view shows a specific band for ADFP in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.17 mg/ml

Perilipin-2/ADFP Antibody Summary

Background
ADFP (aka adipophilin) can be found on the surface of lipid droplets in most mammalian cell types. ADFP is a major protein component of lipid storage droplets. Lipid droplet metabolism is likely controlled by the coat proteins of the perilipin family, which include ADFP, perilipin, TIP47, S3-12, and PAT-1. Perilipin family proteins localize to lipid droplets, either constitutively (perilipin, ADFP) or in response to lipogenic and/or lipolytic stimuli (TIP47, S3-12, and PAT-1).
Immunogen
A synthetic peptide made to a C-terminal region of mouse ADFP (within residues 350-425) [Swiss-Prot# P43883]
Localization
Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLIN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This ADFP antibody can be used for Western blot and Immunofluorescence/Immunocytochemistry. In Western blot a band is observed at approx. 51 kDa. In ICC/IF, membrane staining was observed in HeLa cells. In IHC-P, staining was observed on the membrane of mouse liver cells Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Adrenal Lysate (NB820-59318)
Liver Lysate (NB820-59232)
Liver Lysate (NB820-59662)
Perilipin-2/ADFP Lysate (NBL1-07334)
Control Peptide
Perilipin-2/ADFP Peptide (NB110-40877PEP)
Publications
Read Publications using
NB110-40877 in the following applications:

Reactivity Notes

Immunogen has 88% sequence identity with rat ADFP. Customer feedback has been negative on trout liver.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.17 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Perilipin-2/ADFP Antibody

  • ADFP
  • Adipophilin
  • Adipose differentiation-related proteinadipophilin
  • ADRP
  • MGC10598
  • perilipin 2
  • Perilipin2
  • Perilipin-2
  • PLIN2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Perilipin-2/ADFP Antibody (NB110-40877)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
WB
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(5)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB110-40877 Applications Species
Li J, Mihalcioiu M, Li L et al. Vitamin D prevents lipid accumulation in murine muscle through regulation of PPARg and perilipin-2 expression. J. Steroid Biochem. Mol. Biol. 2017 Oct 14 [PMID: 29037825] (Mouse) Mouse
Vigelso A, Gram M, Wiuff C et al. Effects of immobilization and aerobic training on proteins related to intramuscular substrate storage and metabolism in young and older men. Eur. J. Appl. Physiol. 2015 Dec 01 [PMID: 26626913] (WB, Human) WB Human
Nishikawa K, Iwaya K, Kinoshita M et al. Resveratrol increases CD68(+) Kupffer cells co-localized with adipose differentiation-related protein (ADFP) and ameliorates high-fat-diet-induced fatty liver in mice Mol Nutr Food Res. 2015 Feb 12 [PMID: 25677089] (WB, Mouse)

Details:
Perilipin-2/ADFP antibody (0.5 ug/ml) used for WB on mouse liver extract (Fig. 4).
WB Mouse
Bindesboll C, Berg O, Arntsen B et al. Fatty acids regulate perilipin5 in muscle by activating PPARdelta. J Lipid Res 2013 Jul [PMID: 23606724]
Lige B, Sampels V, Coppens I. Characterization of a second sterol-esterifying enzyme in Toxoplasma highlights the importance of cholesterol storage pathways for the parasite. Mol Microbiol 2013 Feb 3 [PMID: 23374239] (ICC/IF, Mouse) ICC/IF Mouse
Chikka MR, McCabe DD, Tyra HM, Rutkowski DT. C/EBP Homologous Protein (CHOP) Contributes to Suppression of Metabolic Genes During ER Stress in the Liver. J Biol Chem 2012 Dec 31 [PMID: 23281479] (IHC-P, Mouse) IHC-P Mouse
Son S-H, Goo Y-H, Chang BH, Paul A. Perilipin 2 (PLIN2)-Deficiency Does Not Increase Cholesterol-Induced Toxicity in Macrophages PLoS ONE 2012 [PMID: 22427949] (WB, Mouse) WB Mouse
Wohlers LM, Jackson KC, Spangenburg EE. Lipolytic signaling in response to acute exercise is altered in female mice following ovariectomy. J Cell Biochem. 2011 Aug 3. [PMID: 21815195]

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Perilipin-2/ADFP Antibody (NB110-40877). (Showing 1 - 2 of 2 FAQs).

  1. I would appreciate if you are able to provide the exact amino acid sequence of the immunogens. The customer would like to check the percentage alignment with the chicken protein to determine which of the antiodies woudl be better to try in this species.
    • I am unable to provide you with this information however I can tell you that there is a 71% homology between the chicken protein and human protein. Our lab only recommends use if the homology is 85% or above.
  2. I went trought your product datasheet, but I didn't find any information about the dilution I have to use for the western blot and if it's in 5% milk-TBST or other blocking buffer. Could you please provide me those information?
    • For our product NB110-40877, we recommend a starting dilution of 2 ug/ml for western blot. Our recommended diluent buffer is 5% nonfat milk in TBST.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-40877AF405
Alexa Fluor 488 Labeled NB110-40877AF488
Alexa Fluor 647 Labeled NB110-40877AF647
Alexa Fluor 700 Labeled NB110-40877AF700
Biotin Labeled NB110-40877B
DyLight 405 Labeled NB110-40877V
DyLight 488 Labeled NB110-40877G
DyLight 550 Labeled NB110-40877R
DyLight 650 Labeled NB110-40877C
FITC Labeled NB110-40877F
HRP Labeled NB110-40877H

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Bioinformatics Tool for Perilipin-2/ADFP Antibody (NB110-40877)

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Blogs on Perilipin-2/ADFP.

The affects of Perilipin 2 on diet and metabolism
Perilipin 2 belongs to the Perilipin family, which consists of proteins that coat intracellular lipid storage droplets. Perilipin 2 in particular is involved in lipid globule surface membrane composition, and has also been implicated in the develo...  Read full blog post.

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Bioinformatics

Gene Symbol PLIN2
Entrez
Uniprot