Perilipin-2/ADFP Antibody - BSA Free


Orthogonal Strategies: Dual RNAscope ISH-IHC: Perilipin-2/ADFP Antibody [NB110-40877] - Formalin-fixed paraffin-embedded tissue sections of mouse intestine were probed for Perilipin-2/ADFP mRNA (ACD RNAScope more
Simple Western: Perilipin-2/ADFP Antibody [NB110-40877] - Simple Western lane view shows a specific band for ADFP in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 more
Biological Strategies: Western Blot: Perilipin-2/ADFP Antibody [NB110-40877] - Oleic acid increasesI2 oxidation, glucose utilization and expression of genes involved in LCFA metabolism in U138 GBM cells. Cropped more
Immunohistochemistry: Perilipin-2/ADFP Antibody [NB110-40877] - ADFP antibody was tested in mouse liver using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells more
Western Blot: Perilipin-2/ADFP Antibody [NB110-40877] - Detection of murine ADFP in mouse liver lysate.
Immunocytochemistry/ Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells more
Immunocytochemistry/ Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells more

Product Details

Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC
BSA Free
1.0 mg/ml

Order Details

Perilipin-2/ADFP Antibody - BSA Free Summary

A synthetic peptide made to a C-terminal region of mouse ADFP (within residues 350-425) [Swiss-Prot# P43883]
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Dual RNAscope ISH-IHC
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Simple Western 1:50
  • Western Blot 2 ug/ml
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:35041644) This ADFP antibody can be used for Western blot and Immunofluorescence/Immunocytochemistry. In Western blot a band is observed at approx. 51 kDa. In ICC/IF, membrane staining was observed in HeLa cells. In IHC-P, staining was observed on the membrane of mouse liver cells Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human Adrenal Whole Tissue Lysate (Adult Whole Liver Cirrhosis)
Human Liver Whole Tissue Lysate (Adult Whole Normal)
Mouse Liver Whole Tissue Lysate (Adult Whole Normal)
Perilipin-2/ADFP Overexpression Lysate
Control Peptide
Perilipin-2/ADFP Protein (NB110-40877PEP)
Reviewed Applications
Read 1 Review rated 5
NB110-40877 in the following applications:

Read Publications using
NB110-40877 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 29053516). Use in Bovine reported in scientific literature (PMID:32647143).

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for Perilipin-2/ADFP Antibody - BSA Free

  • ADFP
  • Adipophilin
  • Adipose differentiation-related proteinadipophilin
  • ADRP
  • MGC10598
  • perilipin 2
  • Perilipin2
  • Perilipin-2
  • PLIN2


Perilipin-2/Plin-2, also known as adipose differentiation-related protein (ADFP), ADRP, and adipophilin, is a member of the perilipin family of lipid droplet (LD) proteins that functions in lipid accumulation, lipid storage, and LD formation (1,2). Perilipin-2 is ubiquitously expressed and is the major LD protein of the liver (1-3). Human perilipin-2 is 437 amino acids (aa) in length with a theoretical molecular weight of 48 kDa (1,4). Human and mouse perilipin-2 share 81.8% sequence identity. One defining structural feature of perilipin-2 and other LD-related proteins is formation of amphipathic alpha helices (1,3,5). The perilipin-2 protein contains a PAT (perilipin A, ADRP, TIP47) domain, a 11-mer repeat motif, and a 4-helix bundle (1,5). The PAT domain has a role in triglyceride (TG) stabilization, the 11-mer repeat motif functions in cytoplasmic LD binding, and the 4-helix bundle facilitates membrane binding (5). Unlike LDs coated with other perilipin family members (e.g., perilipin-1 and perilipin-5), LDs coated with perilipin-2 are more permissive to lipolysis and protective of lipids and TGs (1-3). Studies have revealed that perilipin-2 knockout (KO) in mice is associated with resistance to fatty liver disease and diet-induced obesity (1). On the other hand, overexpression of perilipin-2 causes increased lipid and TG accumulation, which is a feature of many metabolic, cardiovascular, and age-related diseases (2). Additional research has found higher levels of perilipin-2 is associated with certain cancers such as colorectal cancer and renal carcinomas, and it may be a useful biomarker for detection (2). Though more research is needed, it is possible that inhibition or blocking of perilipin-2 may be a key strategy to combatting several pathologies (2).


1. Itabe H, Yamaguchi T, Nimura S, Sasabe N. Perilipins: a diversity of intracellular lipid droplet proteins. Lipids Health Dis. 2017;16(1):83.

2. Conte M, Franceschi C, Sandri M, Salvioli S. Perilipin 2 and Age-Related Metabolic Diseases: A New Perspective. Trends Endocrinol Metab. 2016;27(12):893-903.

3. Sztalryd C, Brasaemle DL. The perilipin family of lipid droplet proteins: Gatekeepers of intracellular lipolysis. Biochim Biophys Acta Mol Cell Biol Lipids. 2017;1862(10 Pt B):1221-1232.

4. Uniprot (Q99541)

5. Chong BM, Reigan P, Mayle-Combs KD, Orlicky DJ, McManaman JL. Determinants of adipophilin function in milk lipid formation and secretion. Trends Endocrinol Metab. 2011;22(6):211-217. https://10.1016/j.tem.2011.04.003


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Perilipin-2/ADFP Antibody (NB110-40877)(49)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Bovine.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.

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Showing Publications 1 - 10 of 49. Show All 49 Publications.
Publications using NB110-40877 Applications Species
Ibayashi M, Aizawa R, Mitsui J, Tsukamoto S Lipid droplet synthesis is associated with angiogenesis in mouse ovarian follicles Biology of reproduction 2022-12-28 [PMID: 36579469]
Wang N, Wang M, Jeevaratnam S et al. Opposing effects of apoE2 and apoE4 on microglial activation and lipid metabolism in response to demyelination Molecular neurodegeneration 2022-11-23 [PMID: 36419137] (IF/IHC, Mouse) IF/IHC Mouse
Lin M, Ku AT, Dong J et al. STAT5 confers lactogenic properties in breast tumorigenesis and restricts metastatic potential Oncogene 2022-10-19 [PMID: 36261627] (IF/IHC, Mouse) IF/IHC Mouse
Fraunhoffer NA, Abuelafia AM, Bigonnet M et al. Multi-omics data integration and modeling unravels new mechanisms for pancreatic cancer and improves prognostic prediction NPJ precision oncology 2022-08-17 [PMID: 35978026] (IHC-P, Human)

Dilutions: 1:100
IHC-P Human
Liu Q, Xiao JJ, Wang S et al. Paraoxonase 1 Ameliorates Renal Lipotoxicity by Activating Lipophagy and Inhibiting Pyroptosis The American journal of pathology 2022-08-10 [PMID: 35963464]
Goossens P, Lu C, Cao J et al. Integrating multiplex immunofluorescent and mass spectrometry imaging to map myeloid heterogeneity in its metabolic and cellular context Cell metabolism 2022-07-12 [PMID: 35858629] (IHC-Fr, Mouse) IHC-Fr Mouse
Wang Z, Wu Q, Wang H et al. Diosgenin protects against podocyte injury in early phase of diabetic nephropathy through regulating SIRT6 Phytomedicine : international journal of phytotherapy and phytopharmacology 2022-06-13 [PMID: 35728388] (IF/IHC, Mouse) IF/IHC Mouse
Antony R, Aby K, Gao H et al. UCHL1 Regulates Lipid and Perilipin 2 Level in Skeletal Muscle Frontiers in physiology 2022-04-07 [PMID: 35464088] (WB, Mouse) WB Mouse
Asif S, Kim RY, Fatica T et al. Hmgcs2-mediated ketogenesis modulates high-fat diet-induced hepatosteatosis Molecular metabolism 2022-04-12 [PMID: 35421611] (IHC-P, Mouse) IHC-P Mouse
Show All 49 Publications.

Review for Perilipin-2/ADFP Antibody (NB110-40877) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB110-40877:
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Images Ratings Applications Species Date Details
Western Blot Perilipin-2/ADFP NB110-40877
reviewed by:
Verified Customer
WB Rat 06/16/2020


ApplicationWestern Blot
Sample TestedLiver whole cell extract,Liver whole tissue homogenate, Sample Amount: 50ug


CommentsConcentration 1:1000, over night
Secondary antibody: 1: 20000

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Perilipin-2/ADFP Antibody (NB110-40877). (Showing 1 - 2 of 2 FAQs).

  1. I would appreciate if you are able to provide the exact amino acid sequence of the immunogens. The customer would like to check the percentage alignment with the chicken protein to determine which of the antiodies woudl be better to try in this species.
    • I am unable to provide you with this information however I can tell you that there is a 71% homology between the chicken protein and human protein. Our lab only recommends use if the homology is 85% or above.
  2. I went trought your product datasheet, but I didn't find any information about the dilution I have to use for the western blot and if it's in 5% milk-TBST or other blocking buffer. Could you please provide me those information?
    • For our product NB110-40877, we recommend a starting dilution of 2 ug/ml for western blot. Our recommended diluent buffer is 5% nonfat milk in TBST.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Blogs on Perilipin-2/ADFP.

The affects of Perilipin 2 on diet and metabolism
Perilipin 2 belongs to the Perilipin family, which consists of proteins that coat intracellular lipid storage droplets. Perilipin 2 in particular is involved in lipid globule surface membrane composition, and has also been implicated in the develo...  Read full blog post.

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Recent Reviews


Verified Customer
Application: WB
Species: Rat


Gene Symbol PLIN2