Perilipin-2/ADFP Antibody

Images

 
Orthogonal Strategies: Dual RNAscope ISH-IHC: Perilipin-2/ADFP Antibody [NB110-40877] - Formalin-fixed paraffin-embedded tissue sections of mouse intestine were probed for Perilipin-2/ADFP mRNA (ACD RNAScope ...read more
Simple Western: Perilipin-2/ADFP Antibody [NB110-40877] - Simple Western lane view shows a specific band for ADFP in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 ...read more
Biological Strategies: Western Blot: Perilipin-2/ADFP Antibody [NB110-40877] - Oleic acid increasesI2 oxidation, glucose utilization and expression of genes involved in LCFA metabolism in U138 GBM cells. Cropped ...read more
Immunohistochemistry: Perilipin-2/ADFP Antibody [NB110-40877] - ADFP antibody was tested in mouse liver using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells ...read more
Western Blot: Perilipin-2/ADFP Antibody [NB110-40877] - Detection of murine ADFP in mouse liver lysate.
Immunocytochemistry/ Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more
Immunocytochemistry/ Immunofluorescence: Perilipin-2/ADFP Antibody [NB110-40877] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, Dual ISH-IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Perilipin-2/ADFP Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal region of mouse ADFP (within residues 350-425) [Swiss-Prot# P43883]
Localization
Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLIN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
  • Simple Western 1:50
  • Western Blot 2 ug/ml
Application Notes
This ADFP antibody can be used for Western blot and Immunofluorescence/Immunocytochemistry. In Western blot a band is observed at approx. 51 kDa. In ICC/IF, membrane staining was observed in HeLa cells. In IHC-P, staining was observed on the membrane of mouse liver cells Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Adrenal Whole Tissue Lysate (Adult Whole Liver Cirrhosis)
Human Liver Whole Tissue Lysate (Adult Whole Normal)
Mouse Liver Whole Tissue Lysate (Adult Whole Normal)
Perilipin-2/ADFP Overexpression Lysate
Control Peptide
Perilipin-2/ADFP Peptide (NB110-40877PEP)
Reviewed Applications
Read 1 Review rated 5
using
NB110-40877 in the following applications:

Publications
Read Publications using
NB110-40877 in the following applications:

  • 1 publication
  • IHC
    4 publications
  • 2 publications
  • WB
    14 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 29053516). Use in Bovine reported in scientific literature (PMID:32647143).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Perilipin-2/ADFP Antibody

  • ADFP
  • Adipophilin
  • Adipose differentiation-related proteinadipophilin
  • ADRP
  • MGC10598
  • perilipin 2
  • Perilipin2
  • Perilipin-2
  • PLIN2

Background

ADFP (aka adipophilin) can be found on the surface of lipid droplets in most mammalian cell types. ADFP is a major protein component of lipid storage droplets. Lipid droplet metabolism is likely controlled by the coat proteins of the perilipin family, which include ADFP, perilipin, TIP47, S3-12, and PAT-1. Perilipin family proteins localize to lipid droplets, either constitutively (perilipin, ADFP) or in response to lipogenic and/or lipolytic stimuli (TIP47, S3-12, and PAT-1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Perilipin-2/ADFP Antibody (NB110-40877)(30)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Bovine.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC
(4)
IHC-P
(2)
WB
(14)
All Applications
Filter By Species
Human
(8)
Mouse
(16)
Rat
(2)
Bovine
(1)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using NB110-40877 Applications Species
Qi G, Mi Y, Shi X, et al. ApoE4 Impairs Neuron-Astrocyte Coupling of Fatty Acid Metabolism Cell reports Jan 5 2021 [PMID: 33406436] (Human) Human
Lee LL, Khakoo AY, Chintalgattu V Cardiac pericytes function as key vasoactive cells to regulate homeostasis and disease FEBS Open Bio Nov 1 2020 [PMID: 33135334] (WB, Mouse)

Details:
C57/BL6J mouse coronary smooth muscle cells underwent Western blot analysis.
WB Mouse
Farmer BC, Kluemper J, Johnson LA. Apolipoprotein E4 Alters Astrocyte Fatty Acid Metabolism and Lipid Droplet Formation Cells Feb 20 2019 [PMID: 30791549] (WB) WB
Talbott HA, Plewes MR, Krause C et al. Formation and characterization of lipid droplets of the bovine corpus luteum Sci Rep Jul 9 2020 [PMID: 32647143] (WB, Bovine) WB Bovine
Mayagoitia K, Shin SD, Rubini M et al. Short-term exposure to dietary cholesterol is associated with downregulation of interleukin-15, reduced thigmotaxis and memory impairment in mice Behav. Brain Res. Jun 22 2020 [PMID: 32585301] (IHC, Mouse) IHC Mouse
Chen L, Ma WL, Cheng WC et al. Targeting lipid droplet lysophosphatidylcholine for cisplatin chemotherapy J. Cell. Mol. Med. Jun 16 2020 [PMID: 32543783] (WB, Human) WB Human
Milutinovic DV, Brkljacic J, Teofilovic A et al. Chronic Stress Potentiates High Fructose-Induced Lipogenesis in Rat Liver and Kidney Mol Nutr Food Res May 7 2020 [PMID: 32379936] (Rat) Rat
TaIb B, Aboussalah AM, Moniruzzaman M et al. Lipid accumulation and oxidation in glioblastoma multiforme Sci Rep Dec 20 2019 [PMID: 31863022] (WB, Human) WB Human
Helsley RN, Varadharajan V, Brown AL et al. Obesity-linked suppression of membrane-bound O-acyltransferase 7 (MBOAT7) drives non-alcoholic fatty liver disease Elife Oct 17 2019 [PMID: 31621579] (Mouse) Mouse
Show All 30 Publications.

Review for Perilipin-2/ADFP Antibody (NB110-40877) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB110-40877:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Perilipin-2/ADFP NB110-40877
Enlarge
5
reviewed by:
Natasa Velickovic
WB Rat 06/16/2020
View

Summary

ApplicationWestern Blot
Sample TestedLiver whole cell extract,Liver whole tissue homogenate, Sample Amount: 50ug
SpeciesRat
LotF-3

Comments

CommentsConcentration 1:1000, over night
Secondary antibody: 1: 20000

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Perilipin-2/ADFP Antibody (NB110-40877). (Showing 1 - 2 of 2 FAQs).

  1. I would appreciate if you are able to provide the exact amino acid sequence of the immunogens. The customer would like to check the percentage alignment with the chicken protein to determine which of the antiodies woudl be better to try in this species.
    • I am unable to provide you with this information however I can tell you that there is a 71% homology between the chicken protein and human protein. Our lab only recommends use if the homology is 85% or above.
  2. I went trought your product datasheet, but I didn't find any information about the dilution I have to use for the western blot and if it's in 5% milk-TBST or other blocking buffer. Could you please provide me those information?
    • For our product NB110-40877, we recommend a starting dilution of 2 ug/ml for western blot. Our recommended diluent buffer is 5% nonfat milk in TBST.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-40877AF350
Alexa Fluor 405 NB110-40877AF405
Alexa Fluor 488 NB110-40877AF488
Alexa Fluor 532 NB110-40877AF532
Alexa Fluor 594 NB110-40877AF594
Alexa Fluor 647 NB110-40877AF647
Alexa Fluor 700 NB110-40877AF700
Alexa Fluor 750 NB110-40877AF750
Biotin NB110-40877B
DyLight 350 NB110-40877UV
DyLight 405 NB110-40877V
DyLight 488 NB110-40877G
DyLight 550 NB110-40877R
DyLight 594 NB110-40877DL594
DyLight 650 NB110-40877C
DyLight 680 NB110-40877FR
DyLight 755 NB110-40877IR
FITC NB110-40877F
HRP NB110-40877H
Janelia Fluor 549 NB110-40877JF549
Janelia Fluor 646 NB110-40877JF646

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Bioinformatics Tool for Perilipin-2/ADFP Antibody (NB110-40877)

Discover related pathways, diseases and genes to Perilipin-2/ADFP Antibody (NB110-40877). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Perilipin-2/ADFP.

The affects of Perilipin 2 on diet and metabolism
Perilipin 2 belongs to the Perilipin family, which consists of proteins that coat intracellular lipid storage droplets. Perilipin 2 in particular is involved in lipid globule surface membrane composition, and has also been implicated in the develo...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
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Natasa Velickovic
06/16/2020
Application: WB
Species: Rat

Bioinformatics

Gene Symbol PLIN2
Entrez
Uniprot