FoxC2 Antibody

FoxC2 was detected in immersion fixed frozen sections of mouse embryo (E15.5) using Sheep Anti-Mouse FoxC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6989) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to periocular mesenchyme. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

FoxC2 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse FoxC2
  Ala412-Tyr494
  Accession # Q61850
• Specificity: Detects mouse FoxC2 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) FoxC2 is observed and less than 3% cross-reactivity with rhFoxF2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: FOXC2
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxC2 Antibody

• Fkh14
• FKHL14LD
• forkhead box C2 (MFH-1, mesenchyme forkhead 1)
• forkhead, Drosophila, homolog-like 14
• Forkhead-related protein FKHL14
• FoxC2
• LD
• Mesenchyme fork head protein 1
• mesenchyme forkhead 1
• MFH-1 protein
• MFH1
• MFH-1
• MFH1forkhead box protein C2
• Transcription factor FKH-14

Background

FOXC2 (Forkhead box protein C2; also BF-3, FKH-14 and MFH-1) is a 64-66 kDa member of the winged helix transcription factor gene family. It is widely expressed, including in endothelium where it induces the appearance of CXCR4 and integrin beta 3, two molecules essential to cell migration. High-calorie diets also induce FOXC2 in adipocytes, inducing a brown-fat like phenotype. Mouse FOXC2 is 494 amino acids (aa) in length. It contains a forkhead DNA binding domain (aa 71-148), a short poly-Arg segment (aa 162-166), one His-rich region (aa 386-395) and an Ala/Pro-rich domain (aa 396-415). There are seven potential Ser/Thr phosphorylation sites. Over aa 412-494, mouse FOXC2 shares 99% and 87% aa identity with rat and human FOXC2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FoxC2 Antibody (AF6989)(8)

Publications using AF6989

• P Flora, MY Li, PM Galbo, M Astorkia, D Zheng, E Ezhkova Polycomb repressive complex 2 in adult hair follicle stem cells is dispensable for hair regeneration PloS Genetics, 2021-12-14;17(12):e1009948. 2021-12-14 [PMID: 34905545] (IHC, Transgenic Mouse)
• MM Mikedis, Y Fan, PK Nicholls, T Endo, EK Jackson, SA Cobb, DG de Rooij, DC Page DAZL mediates a broad translational program regulating expansion and differentiation of spermatogonial progenitors Elife, 2020-07-20;9(0):. 2020-07-20 [PMID: 32686646] (IHC-P, Mouse)
• K Nonomura, V Lukacs, DT Sweet, LM Goddard, A Kanie, T Whitwam, SS Ranade, T Fujimori, ML Kahn, A Patapoutia Mechanically activated ion channel PIEZO1 is required for lymphatic valve formation Proc. Natl. Acad. Sci. U.S.A., 2018-11-27;0(0):. 2018-11-27 [PMID: 30482854] (IHC-Fr, Mouse)
• B Cha, X Geng, MR Mahamud, JY Zhang, L Chen, W Kim, EH Jho, Y Kim, D Choi, JB Dixon, H Chen, YK Hong, L Olson, TH Kim, BJ Merrill, MJ Davis, RS Srinivasan Complementary Wnt Sources Regulate Lymphatic Vascular Development via PROX1-Dependent Wnt/?-Catenin Signaling Cell Rep, 2018-10-16;25(3):571-584.e5. 2018-10-16 [PMID: 30332639] (IHC, Western Blot, Human, Mouse)
• A Milasan, G Jean, F Dallaire, JC Tardif, Y Merhi, M Sorci-Thom, C Martel Apolipoprotein A-I Modulates Atherosclerosis Through Lymphatic Vessel-Dependent Mechanisms in Mice J Am Heart Assoc, 2017-09-22;6(9):. 2017-09-22 [PMID: 28939717] (IHC, Mouse)
• Boksik Cha Mechanotransduction activates canonical Wnt/?-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves Genes Dev, 2016-06-16;30(12):1454-69. 2016-06-16 [PMID: 27313318] (IHC-Fr, Western Blot, Human, Mouse)
• Sweet D, Jimenez J, Chang J, Hess P, Mericko-Ishizuka P, Fu J, Xia L, Davies P, Kahn M Lymph flow regulates collecting lymphatic vessel maturation in vivo. J Clin Invest, 2015-07-27;125(8):2995-3007. 2015-07-27 [PMID: 26214523] (IHC-P, Mouse)
• Park D, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong K, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong Y, Koh G Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity. J Clin Invest, 2014-07-25;124(9):3960-74. 2014-07-25 [PMID: 25061877] (IHC, Mouse)

Bioinformatics

• Gene Symbol: FOXC2
• Uniprot:
  • Q61850(Mouse)