Fatty Acid Synthase/FASN Antibody

Images

 
Western Blot: Fatty Acid Synthase Antibody [NB400-114] - Expression of Fatty Acid Synthase in chicken hypothalamus. A: total RNA (1 ug) isolated from different tissues (brain, hypothalamus, liver and muscle) was ...read more
Immunocytochemistry/ Immunofluorescence: Fatty Acid Synthase Antibody [NB400-114] - The Fas antibody was tested in MCF-7 cells at a 1:2000 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were ...read more
Immunohistochemistry-Paraffin: Fatty Acid Synthase Antibody [NB400-114] - Immunohistochemical localization of FAS in chicken hypothalmus. Paraffin sections were obtained from 3-wk-old broiler chickens.
Western Blot: Fatty Acid Synthase Antibody [NB400-114] - Analysis of Fatty Acid Synthase, using NB400-114. Samples: 50 ug of total mouse liver lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Fe, Ha, Pl, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Fatty Acid Synthase/FASN Antibody Summary

Immunogen
A synthetic peptide, conjugated to KLH, made near the N-terminus of mouse FAS. [Swiss-Prot# P19096]
Localization
Cytoplasmic
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FASN
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:2000
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin 1:500
  • Immunoprecipitation 1:100
Application Notes
This Fatty Acid Synthase antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation and Western Blot where a band at ~272 kDa is observed. May see 1 or 2 minor cross-reacting lower MW bands in liver tissue. In ICC/IF cytoplasmic staining can be seen in MCF7 cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
272 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Liver Lysate (NB820-59662)
Fatty Acid Synthase/FASN Lysate (NBP2-66058)
Reviewed Applications
Read 2 Reviews rated 4
using
NB400-114 in the following applications:

Publications
Read Publications using
NB400-114 in the following applications:

  • 2 publications
  • IHC
    1 publication
  • 2 publications
  • WB
    11 publications

Reactivity Notes

Human, pig, rat, monkey, chicken, mouse and hamster. Plant reactivity reported in scientific literature (PMID: 24649190). Feline reactivity reported in scientific literature (PMID: 28871635).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Fatty Acid Synthase/FASN Antibody

  • EC 2.3.1
  • EC 2.3.1.85
  • EC 6.3.3.1
  • FASN
  • FASshort chain dehydrogenase/reductase family 27X, member 1
  • Fatty Acid Synthase
  • MGC14367
  • MGC15706
  • OA-519
  • SDR27X1

Background

Fatty acid synthetase (FASN) is a key player of fatty acid biosynthetic pathway and it catalyzes formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH (de novo lipid synthesis). Localized in cytoplasm and melanosomes, FASN is expressed at low level in normal adult tissues; however, it is significantly upregulated in cancer cells. The FASN expression is regulated by growth factors/growth factor receptors (EGFR and HER2) and steroid hormone /steroid hormone receptors (ER, AR and progesterone receptor). Downstream of the receptors, PI3K-AKT and MAPK signaling mediate FASN expression through SREBP-1c. FASN expression is also controlled by p53 family proteins and SPOT14, lipogenesis-related nuclear protein generally overexpressed in breast cancer. Extracellular stresses including hypoxia and low pH also induce the FASN expression in cancer cells and USP2a stabilises FASN alongwith promotion of its mTOR-dependent selective translational induction. FASN activation provides rapidly proliferating tumor cells sufficient amount of lipids for membrane biogenesis and confers growth/survival advantage possibly acting as a metabolic oncogene. Because FASN-inhibitors are selectively toxic to tumor cells, it has emerged as an attractive target for cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-582
Species: Hu, Mu, Rt, Ca, Ch, SyHa, Ha
Applications: WB, Simple Western, IHC
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
AF6898
Species: Hu, Mu, Rt
Applications: WB
MAB126
Species: Hu
Applications: ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP2-22106
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB100-56098
Species: Hu, Mu, Ca
Applications: WB, IHC, IHC-P, IP
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NBP1-26393
Species: Hu
Applications: WB, ICC/IF
NB100-56116
Species: Hu, Mu, Rt, Ge
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB300-537
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-28566
Species: Hu, Mu, Rt, Bv, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P

Publications for Fatty Acid Synthase/FASN Antibody (NB400-114)(19)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Chicken, Feline, Hamster, Plant.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(2)
IHC
(1)
IHC-P
(2)
WB
(11)
All Applications
Filter By Species
Human
(3)
Mouse
(5)
Rat
(1)
Chicken
(1)
Feline
(1)
Hamster
(1)
Plant
(1)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NB400-114 Applications Species
Walz JZ, Saha J, Arora A et al. Fatty acid synthase as a potential therapeutic target in feline oral squamous cell carcinoma Vet Comp Oncol 2017 Sep 04 [PMID: 28871635] (WB, Feline) WB Feline
Park SH, Sung YY, Jang S et al. The Korean herbal medicine, Do In Seung Gi-Tang, attenuates atherosclerosis via AMPK in high-fat diet-induced ApoE(-/-) mice. BMC Complement Altern Med. Sep 8 2016 [PMID: 27608856] (IHC, Mouse) IHC Mouse
Chen CC, Chuang WT, Lin AH et al. Andrographolide inhibits adipogenesis of 3T3-L1 cells by suppressing C/EBPbeta expression and activation. Toxicol. Appl. Pharmacol. Sep 15 2016 [PMID: 27475717] (ICC/IF, Mouse) ICC/IF Mouse
Karlas A, Berre S, Couderc T et al. A human genome-wide loss-of-function screen identifies effective chikungunya antiviral drugs. Nat Commun. May 14 2016 [PMID: 27177310] (WB, ICC/IF, Human) WB, ICC/IF Human
Gang X, Yang Y, Zhong J et al. P300 acetyltransferase regulates fatty acid synthase expression, lipid metabolism and prostate cancer growth. Oncotarget 2016 Mar 22 [PMID: 26934656] (WB) WB
Kim K, Cho YM, Park BH et al. Histological and immunohistochemical markers for progression prediction in transurethrally resected high-grade non-muscle invasive bladder cancer Int J Clin Exp Pathol. 2015 [PMID: 25755769] (IHC-P, Human)

Details:
Fatty Acid Synthase/FASN antibody used for IHC-P on formalin-fixed paraffin-embedded TMA sections that were creataed from human bladder cancer tissue blocks of TURBT specimens. The primary antibody was used at 1:500 dilution and the staining protocol involved automated system BenchMark XT from Ventana Medical Systems and an ultraView Universal DAB detection kit. FASN was found to localize to the cytoplasm of tumor cells and a representative image is shown in Figure 1F.
IHC-P Human
Eser PO, Vanden Heuvel JP, Araujo J et al. Marine- and plant-derived omega-3 fatty acids differentially regulate prostate cancer cell proliferation. Mol Clin Oncol 2013 May [PMID: 24649190] (Plant) Plant
He Y, Li Y, Zhao T et al. Ursolic Acid Inhibits Adipogenesis in 3T3-L1 Adipocytes through LKB1/AMPK Pathway. PLoS One 2013 Jul 26 [PMID: 23922935] (WB, Mouse) WB Mouse
Zhang Wencheng, Wang Qilong, Song Ping, Zou Ming-Hui. Liver kinase b1 is required for white adipose tissue growth and differentiation. Diabetes 2013 [PMID: 23396401] (WB, Mouse) WB Mouse
Grenier E, Garofalo C, Delvin E, Levy E. Modulatory Role of PYY in Transport and Metabolism of Cholesterol in Intestinal Epithelial Cells plos One 2012 [PMID: 22844422] (WB, Human) WB Human
Show All 19 Publications.

Reviews for Fatty Acid Synthase/FASN Antibody (NB400-114) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using NB400-114:
Filter by Applications
IF
(1)
WB
(1)
All Applications
Filter by Species
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Kristine Griffett
IF Mouse 03/12/2013
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse Liver, Xenograft tumors
SpeciesMouse

Blocking

Blocking DetailsPBST + NGS, 1 hour, room temp

Primary Anitbody

Dilution Ratio1:500, over night cold room

Secondary Antibody

Secondary Descriptiondylight conjugated goat-anti rabbit

Details

Fixation DetailsPBST, PBST + NGS, Room Temp
Wash DescriptionPBST x 3 washes x 15 min
 
reviewed by:
Anonymous
WB Rat 12/06/2011
View

Summary

ApplicationWestern Blot
Sample TestedRat
SpeciesRat

Blocking

Blocking DetailsBlocking Buffer: 5% Milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: Rabbit

Details

Detection NotesDetection Method: ECL

Product General Protocols

View specific protocols for Fatty Acid Synthase/FASN Antibody (NB400-114): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Fatty Acid Synthase/FASN Antibody (NB400-114). (Showing 1 - 1 of 1 FAQs).

  1. We are interested in your item, NB400-114. Could you please provide us specific protocol of NB400-114 for IHC-P?
    • If you were requesting the protocol for WB, then we have a special one for this antibody because it is highly acetylated and tightly associated with membrane. However, we don't have any special IHC-P protocol for NB400-114, and the general one can be used: view protocol. (If there was a special IHC-P protocol for this antibody, it would have posted on the webpage for this product; but it does not) The only thing you will concern for using this general IHC-P would be how to do antigen retrieval. Human Protein Atlas shows that for performing antigen retrieval during IHC-P experiments ProteinAtlas site, HIER pH 6 can be applied. For this, both human and mouse tissues are applicable to HIER pH 6.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Fatty Acid Synthase/FASN Antibody (NB400-114)

Discover related pathways, diseases and genes to Fatty Acid Synthase/FASN Antibody (NB400-114). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Fatty Acid Synthase/FASN Antibody (NB400-114)

Discover more about diseases related to Fatty Acid Synthase/FASN Antibody (NB400-114).
 

Pathways for Fatty Acid Synthase/FASN Antibody (NB400-114)

View related products by pathway.

PTMs for Fatty Acid Synthase/FASN Antibody (NB400-114)

Learn more about PTMs related to Fatty Acid Synthase/FASN Antibody (NB400-114).
 

Research Areas for Fatty Acid Synthase/FASN Antibody (NB400-114)

Find related products by research area.

Blogs on Fatty Acid Synthase/FASN

There are no specific blogs for Fatty Acid Synthase/FASN, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
Kristine Griffett
03/12/2013
Application: IF
Species: Mouse

 
Anonymous
12/06/2011
Application: WB
Species: Rat

Bioinformatics

Gene Symbol FASN
Entrez
Uniprot