Fatty Acid Synthase/FASN Antibody

Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Expression of Fatty Acid Synthase in chicken hypothalamus. A: total RNA (1 ug) isolated from different tissues (brain, hypothalamus, liver and muscle) was subjected to RT-PCR using specific primers for chicken Fatty Acid Synthase (GenBank accession JO4485) or ribosomal 18S as a control (GenBank accession AF173612) B: tissue lysates (brain, hypothalmus, liver and muscle) were subjected to Western blot as described in MATERIALS AND METHODS. Blots were incubated with anti-Fatty Acid Synthase antibody and revealed by enhanced chemiluminescence. Picture compliments of Dridi S. et al, Am J Physiol Regul Integr Comp Physiol. 2006 Jul;291(1):R138-47. Epub 2006 Feb 2.

Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114] - The Fas antibody was tested in MCF-7 cells at a 1:2000 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.

Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NB400-114] - Immunohistochemical localization of FAS in chicken hypothalmus. Paraffin sections were obtained from 3-wk-old broiler chickens.

Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Analysis of Fatty Acid Synthase, using NB400-114. Samples: 50 ug of total mouse liver lysate.

Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle. Confocal section of CHIKV replicon-infected HeLa cells labelled for FASN, dsRNA and 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms11320), licensed under a CC-BY licence.

Knockdown Validated: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle. Western blot showing silencing efficiency of FASN-, ACC- and ACLY-specific siRNAs on CHIKV replication (n=10 for each data set).

• Reactivity: Hu, Mu, Rt, Po, Ch, Fe, Ha, Pl, Pm
• Applications: WB, ICC/IF, IHC, IHC-P, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Fatty Acid Synthase/FASN Antibody Summary

• Immunogen: A synthetic peptide, conjugated to KLH, made near the N-terminus of mouse FAS. [Swiss-Prot# P19096]
• Localization: Cytoplasmic
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FASN
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:2000
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin 1:500immunoprecipitation 1:100
  • Immunoprecipitation 1:100
  • Knockdown Validated
  • Western Blot 1:1000
• Application Notes: This Fatty Acid Synthase antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation and Western Blot where a band at ~272 kDa is observed. May see 1 or 2 minor cross-reacting lower MW bands in liver tissue. In ICC/IF cytoplasmic staining can be seen in MCF7 cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 272 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Mouse Liver Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Human, pig, rat, monkey, chicken, mouse and hamster. Plant reactivity reported in scientific literature (PMID: 24649190). Feline reactivity reported in scientific literature (PMID: 28871635).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Fatty Acid Synthase/FASN Antibody

• EC 2.3.1
• EC 2.3.1.85
• EC 6.3.3.1
• FASN
• FASshort chain dehydrogenase/reductase family 27X, member 1
• Fatty Acid Synthase
• MGC14367
• MGC15706
• OA-519
• SDR27X1

Background

Fatty acid synthetase (FASN) is a key player of fatty acid biosynthetic pathway and it catalyzes formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH (de novo lipid synthesis). Localized in cytoplasm and melanosomes, FASN is expressed at low level in normal adult tissues; however, it is significantly upregulated in cancer cells. The FASN expression is regulated by growth factors/growth factor receptors (EGFR and HER2) and steroid hormone /steroid hormone receptors (ER, AR and progesterone receptor). Downstream of the receptors, PI3K-AKT and MAPK signaling mediate FASN expression through SREBP-1c. FASN expression is also controlled by p53 family proteins and SPOT14, lipogenesis-related nuclear protein generally overexpressed in breast cancer. Extracellular stresses including hypoxia and low pH also induce the FASN expression in cancer cells and USP2a stabilises FASN alongwith promotion of its mTOR-dependent selective translational induction. FASN activation provides rapidly proliferating tumor cells sufficient amount of lipids for membrane biogenesis and confers growth/survival advantage possibly acting as a metabolic oncogene. Because FASN-inhibitors are selectively toxic to tumor cells, it has emerged as an attractive target for cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fatty Acid Synthase/FASN Antibody (NB400-114)(24)

Publications using NB400-114

• Gupta K, Mukherjee S, Sen S, Sonawane M Coordinated activities of Myosin Vb isoforms and mTOR signaling regulate epithelial cell morphology during development Development (Cambridge, England) 2022-03-15 [PMID: 35299238]
• Han Y, Lee Y, Jang Y et Al. Aspirin Improves Nonalcoholic Fatty Liver Disease and Atherosclerosis through Regulation of the PPARdelta-AMPK-PGC-1 alpha Pathway in Dyslipidemic Conditions BioMed Research International 2020-03-19 [PMID: 32258142] (WB, Mouse, Human)
• Iizuka Y, Chiba K, Kim H et al. Impact of discontinuation of fish oil after pioglitazone-fish oil combination therapy in diabetic KK mice J. Nutr. Biochem. 2019-11-07 [PMID: 31760227] (WB, Mouse)
• Gang, X;Xuan, L;Zhao, X;Lv, Y;Li, F;Wang, Y;Wang, G; Speckle-type POZ protein suppresses lipid accumulation and prostate cancer growth by stabilizing fatty acid synthase Prostate 2019-04-07 [PMID: 30955223] (WB, Human)
• Li MD, Vera NB, Yang Y et al. Adipocyte OGT governs diet-induced hyperphagia and obesity Nat Commun 2018-11-30 [PMID: 30504766] (WB, Mouse)
• Walz JZ, Saha J, Arora A et al. Fatty acid synthase as a potential therapeutic target in feline oral squamous cell carcinoma Vet Comp Oncol 2017-09-04 [PMID: 28871635] (WB, Feline)
• Park SH, Sung YY, Jang S et al. The Korean herbal medicine, Do In Seung Gi-Tang, attenuates atherosclerosis via AMPK in high-fat diet-induced ApoE(-/-) mice. BMC Complement Altern Med. 2016-09-08 [PMID: 27608856] (IF/IHC, Mouse)
• Chen CC, Chuang WT, Lin AH et al. Andrographolide inhibits adipogenesis of 3T3-L1 cells by suppressing C/EBPbeta expression and activation. Toxicol. Appl. Pharmacol. 2016-09-15 [PMID: 27475717] (ICC/IF, Mouse)
• Karlas A, Berre S, Couderc T et al. A human genome-wide loss-of-function screen identifies effective chikungunya antiviral drugs. Nat Commun. 2016-05-14 [PMID: 27177310] (WB, ICC/IF, Human)
• Gang X, Yang Y, Zhong J et al. P300 acetyltransferase regulates fatty acid synthase expression, lipid metabolism and prostate cancer growth. Oncotarget 2016-03-22 [PMID: 26934656] (WB)
• Kim K, Cho YM, Park BH et al. Histological and immunohistochemical markers for progression prediction in transurethrally resected high-grade non-muscle invasive bladder cancer Int J Clin Exp Pathol. 2015-01-01 [PMID: 25755769] (IHC-P, Human)
  
  Details:
  Fatty Acid Synthase/FASN antibody used for IHC-P on formalin-fixed paraffin-embedded TMA sections that were creataed from human bladder cancer tissue blocks of TURBT specimens. The primary antibody was used at 1:500 dilution and the staining protocol involved automated system BenchMark XT from Ventana Medical Systems and an ultraView Universal DAB detection kit. FASN was found to localize to the cytoplasm of tumor cells and a representative image is shown in Figure 1F.
• Eser PO, Vanden Heuvel JP, Araujo J et al. Marine- and plant-derived omega-3 fatty acids differentially regulate prostate cancer cell proliferation. Mol Clin Oncol 2013-05-01 [PMID: 24649190] (Plant)
• He Y, Li Y, Zhao T et al. Ursolic Acid Inhibits Adipogenesis in 3T3-L1 Adipocytes through LKB1/AMPK Pathway. PLoS One 2013-07-26 [PMID: 23922935] (WB, Mouse)
• Zhang Wencheng, Wang Qilong, Song Ping, Zou Ming-Hui. Liver kinase b1 is required for white adipose tissue growth and differentiation. Diabetes 2013-01-01 [PMID: 23396401] (WB, Mouse)
• Grenier E, Garofalo C, Delvin E, Levy E. Modulatory Role of PYY in Transport and Metabolism of Cholesterol in Intestinal Epithelial Cells plos One 2012-01-01 [PMID: 22844422] (WB, Human)
• Oliva J, French SW, Li J et al. Proteasome inhibitor treatment reduced fatty acid, triacylglycerol and cholesterol synthesis Experimental and Molecular Pathology 2012-08-01 [PMID: 22445925] (WB, Rat)
• Chow JD, Jones ME, Prelle K, Simpson ER, Boon WC. A selective estrogen receptor ? agonist ameliorates hepatic steatosis in the male aromatase knockout mouse. J;210(3):323-34. 2011-09-01 [PMID: 21705395] (WB, Mouse)
• Basciano H et al. Metabolic effects of dietary cholesterol in an animal model of insulin resistance and hepatic steatosis. Am J Physiol Endocrinol Metab;297(2):E462-73. 2009-08-01 [PMID: 19509184] (WB)
• Basciano H, Miller A, Baker C et al. LXR{alpha} activation perturbs hepatic insulin signaling stimulates production of apolipoprotein B-containing lipoproteins. Am J Physiol Gastrointest Liver Physiol;297(2):G323-332. 2009-01-01 [PMID: 19497957] (WB, Hamster)
• Bryzgalova G, Lundholm L, Portwood N,et al. Mechanisms of antidiabetogenic and body weight-lowering effects of estrogen in high-fat diet-fed mice. Am J Physiol Endocrinol Metab;295(4):E904-12. 2008-10-01 [PMID: 18697913]
• Vavere, AL et al. 1-11C-acetate as a PET radiopharmaceutical for imaging fatty acid synthase expression in prostate cancer. J Nucl Med;49(2):327-34. 2008-02-01 [PMID: 18199615]
• Heemers, HV et al. Androgen deprivation increases p300 expression in prostate cancer cells. Cancer Res;67(7):3422-30. 2007-04-01 [PMID: 17409453]
• Gao H, Bryzgalova G, Hedman E et al. Long-term administration of estradiol decreases expression of hepatic lipogenic genes and improves insulin sensitivity in ob/ob mice: a possible mechanism is through direct regulation of signal transducer and activator of transcription 3. Mol Endocrinol;20(6):1287-99. 2006-06-01 [PMID: 16627594]
• Dridi S et al. FAS inhibitor cerulenin reduces food intake and melanocortin receptor gene expression without modulating the other (an)orexigenic neuropeptides in chickens. Am J Physiol Regul Integr Comp Physiol;291(1):R138-47. 2006-07-01 [PMID: 16455759] (WB, IHC-P, Chicken)

Reviews for Fatty Acid Synthase/FASN Antibody (NB400-114) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Rat.

reviewed by: Verified Customer IF Mouse 03/12/2013

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse Liver, Xenograft tumors
• Species: Mouse

reviewed by: Chiung-Chi Peng WB Rat 12/06/2011

Summary

• Application: Western Blot
• Sample Tested: Rat
• Species: Rat

FAQs for Fatty Acid Synthase/FASN Antibody (NB400-114). (Showing 1 - 1 of 1 FAQs).

1. We are interested in your item, NB400-114. Could you please provide us specific protocol of NB400-114 for IHC-P?
   • If you were requesting the protocol for WB, then we have a special one for this antibody because it is highly acetylated and tightly associated with membrane. However, we don't have any special IHC-P protocol for NB400-114, and the general one can be used: view protocol. (If there was a special IHC-P protocol for this antibody, it would have posted on the webpage for this product; but it does not) The only thing you will concern for using this general IHC-P would be how to do antigen retrieval. Human Protein Atlas shows that for performing antigen retrieval during IHC-P experiments ProteinAtlas site, HIER pH 6 can be applied. For this, both human and mouse tissues are applicable to HIER pH 6.

Bioinformatics

• Gene Symbol: FASN
• Entrez:
  • 2194 (Human)
  • 14104 (Mouse)
• Uniprot:
  • P19096(Mouse)