Fatty Acid Synthase/FASN Antibody Summary
A synthetic peptide, conjugated to KLH, made near the N-terminus of mouse FAS. [Swiss-Prot# P19096]
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot 1:1000
- Immunocytochemistry/Immunofluorescence 1:2000
- Immunohistochemistry 1:500
- Immunohistochemistry-Paraffin 1:500
- Immunoprecipitation 1:100
This Fatty Acid Synthase antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation and Western Blot where a band at ~272 kDa is observed. May see 1 or 2 minor cross-reacting lower MW bands in liver tissue. In ICC/IF cytoplasmic staining can be seen in MCF7 cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
|Read Publications using
NB400-114 in the following applications:
Human, pig, rat, monkey, chicken, mouse and hamster. Plant reactivity reported in scientific literature (PMID: 24649190). Feline reactivity reported in scientific literature (PMID: 28871635).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Alternate Names for Fatty Acid Synthase/FASN Antibody
- EC 2.3.1
- EC 126.96.36.199
- EC 188.8.131.52
- FASshort chain dehydrogenase/reductase family 27X, member 1
- Fatty Acid Synthase
Fatty acid synthetase (FASN) is a key player of fatty acid biosynthetic pathway and it catalyzes formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH (de novo lipid synthesis). Localized in cytoplasm and melanosomes, FASN is expressed at low level in normal adult tissues; however, it is significantly upregulated in cancer cells. The FASN expression is regulated by growth factors/growth factor receptors (EGFR and HER2) and steroid hormone /steroid hormone receptors (ER, AR and progesterone receptor). Downstream of the receptors, PI3K-AKT and MAPK signaling mediate FASN expression through SREBP-1c. FASN expression is also controlled by p53 family proteins and SPOT14, lipogenesis-related nuclear protein generally overexpressed in breast cancer. Extracellular stresses including hypoxia and low pH also induce the FASN expression in cancer cells and USP2a stabilises FASN alongwith promotion of its mTOR-dependent selective translational induction. FASN activation provides rapidly proliferating tumor cells sufficient amount of lipids for membrane biogenesis and confers growth/survival advantage possibly acting as a metabolic oncogene. Because FASN-inhibitors are selectively toxic to tumor cells, it has emerged as an attractive target for cancer.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Product General Protocols
View specific protocols for Fatty Acid Synthase/FASN Antibody (NB400-114):
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Fatty Acid Synthase/FASN Antibody (NB400-114). (Showing 1 - 1 of 1 FAQs).
We are interested in your item, NB400-114. Could you please provide us specific protocol of NB400-114 for IHC-P?
- If you were requesting the protocol for WB, then we have a special one for this antibody because it is highly acetylated and tightly associated with membrane. However, we don't have any special IHC-P protocol for NB400-114, and the general one can be used: view protocol. (If there was a special IHC-P protocol for this antibody, it would have posted on the webpage for this product; but it does not) The only thing you will concern for using this general IHC-P would be how to do antigen retrieval. Human Protein Atlas shows that for performing antigen retrieval during IHC-P experiments ProteinAtlas site, HIER pH 6 can be applied. For this, both human and mouse tissues are applicable to HIER pH 6.
Positive Control Lysate(s)
Additional Array Products
Bioinformatics Tool for Fatty Acid Synthase/FASN Antibody (NB400-114)
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Blogs on Fatty Acid Synthase/FASN