Perilipin-3/TIP47 Antibody

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Immunocytochemistry/ Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40764] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells ...read more
Western Blot: Perilipin-3/TIP47 Antibody [NB110-40764] - Detection of TIP47 in 3T3 L1 lysate.
Immunocytochemistry/ Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40764] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells ...read more
Western Blot: Perilipin-3/TIP47 Antibody [NB110-40764] - Changes in protein and gene expression relating to lipolysis with a single bout of endurance exercise in human skeletal muscle. Representative blots of PLIN3, ...read more
Immunocytochemistry/ Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40764] - TIP47 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Changes in protein and gene expression relating to lipolysis with a single bout of endurance exercise in human skeletal muscle.A) Representative blots of PLIN3, ATGL, ARF1, ARFRP1 and loading control GAPDH. Image ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, PmSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.00 mg/ml

Order Details

Perilipin-3/TIP47 Antibody Summary

Immunogen
A synthetic peptide made to a region within the C-terminus (within residues 350-435) of the human TIP47 protein. [Swiss-Prot# O60664]
Localization
Cytoplasm. Endosome. Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLIN3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 2 ug/ml
Application Notes
This TIP47 antibody is useful for Western Blot and Immunocytochemistry/Immunofluorescence. In Western Blot analysis, a band is seen at ~47 kDa, representing isoform B in both human (faint) and mouse lysates. In some samples, a ~28 kDa band may be observed which represents a splice isoform. In ICC/IF, endosomal staining was observed in U2OS cells.
Control
Perilipin-3/TIP47 Overexpression Lysate
Control Peptide
Perilipin-3/TIP47 Peptide (NB110-40764PEP)
Publications
Read Publications using
NB110-40764 in the following applications:

  • 3 publications
  • WB
    6 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.00 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Perilipin-3/TIP47 Antibody

  • Cargo selection protein TIP47
  • M6PRBP1
  • M6PRBP1MGC2012
  • Mannose-6-phosphate receptor-binding protein 1
  • perilipin 3,47 kDa MPR-binding protein
  • Perilipin3
  • Perilipin-3
  • Placental protein 17,47 kDa mannose 6-phosphate receptor-binding protein
  • PLIN3
  • PP17
  • PP17MGC11117
  • tail-interacting protein, 47 kD
  • TIP47
  • TIP47mannose-6-phosphate receptor binding protein 1

Background

TIP47 (tail-interacting protein 47) is a member of PAT family of proteins (perilipin, ADRP, S3-12 and OXPAT) which plays a key role in the packaging/storage of neutral lipids and is required for mannose 6-phosphate receptors (M6PR) transport from endosomes to trans-Golgi network (TGN). TIP47 localizes in cytoplasm, endosome membrane as peripheral membrane protein towards cytoplasmic side, core of lipid bodies/ lipid droplet, in lipid sails and in the envelope, and it interacts with M6PR, IGF2R etc. via the cytoplasmic domain. As a cargo protein, TIP47 facilitates the delivery of M6PR from endosomes to TGN where it binds to cytoplasmic domains of cation-independent/-dependent M6PR and translocates to late endosomes by binding to Rab9 GTPase. Like other PAT family members, TIP47 associates with lipid droplets and influence insulin signaling as evidenced by combined knockout of ADRP /TIP47 leading to decreased insulin sensitivity. During HIV-1 infection, TIP47 acts as a cellular cofactor playing a part in Env incorporation, allowing the encounter and physical association between HIV-1 Gag and Env proteins during viral assembly process. TIP47 is identical to pregnancy-related placental tissue protein 17b (PP17b) and is overexpressed in cervical carcinoma patients wherein it is considered a serum tumor marker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Perilipin-3/TIP47 Antibody (NB110-40764)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(3)
WB
(6)
All Applications
Filter By Species
Human
(6)
Mouse
(5)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB110-40764 Applications Species
Menon D, Bhapkar A, Manchandia B et al. ARL8B mediates lipid droplet contact and delivery to lysosomes for lipid remobilization Cell reports 2023-10-31 [PMID: 37777960] (ICC/IF, Human) ICC/IF Human
Bajpeyi S, Apaflo JN, Rosas V et al. Effect of an acute long-duration exercise bout on skeletal muscle lipid droplet morphology, GLUT 4 protein, and perilipin protein expression European journal of applied physiology 2023-06-27 [PMID: 37368137]
Antony R, Aby K, Gao H et al. UCHL1 Regulates Lipid and Perilipin 2 Level in Skeletal Muscle Frontiers in physiology 2022-04-07 [PMID: 35464088] (WB, Mouse) WB Mouse
Whytock KL, Parry SA, Turner MC et al. A high-fat high-calorie diet induces fibre-specific increases in intramuscular triglyceride and perilipin protein expression in human skeletal muscle J. Physiol. (Lond.) 2020-01-20 [PMID: 31958145] (ICC/IF, Human) ICC/IF Human
Helsley RN, Varadharajan V, Brown AL et al. Obesity-linked suppression of membrane-bound O-acyltransferase 7 (MBOAT7) drives non-alcoholic fatty liver disease Elife 2019-10-17 [PMID: 31621579] (Mouse) Mouse
Keenan SN, Meex RC, Lo JCY et al. Perilipin 5 Deletion in Hepatocytes Remodels Lipid Metabolism and Causes Hepatic Insulin Resistance in Mice. Diabetes 2019-01-07 [PMID: 30617219] (WB, Mouse) WB Mouse
Adam M, Heikela H, Sobolewski C, Portius D. Hydroxysteroid (17b) dehydrogenase 13 deficiency triggers hepatic steatosis and inflammation in mice. FASEB J. 2018-01-31 [PMID: 29401633] (WB, Mouse) WB Mouse
Kuo A, Lee MY, Sessa WC. Lipid Droplet Biogenesis and Function in the Endothelium. Circ. Res. 2017-01-24 [PMID: 28119423]
Covington JD, Noland RC, Hebert RC et al. Perilipin 3 Differentially Regulates Skeletal Muscle Lipid Oxidation in Active, Sedentary and Type 2 Diabetic Males. J. Clin. Endocrinol. Metab. 2015-07-14 [PMID: 26171795]
Wolins NE, Quaynor BK, Skinner JR et al. S3-12, Adipophilin, and TIP47 package lipid in adipocytes. J Biol Chem 280(19):19146-55. 2005-05-13 [PMID: 15731108] (WB, ICC/IF, Mouse, Human) WB, ICC/IF Mouse, Human
Show All 14 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PLIN3