Perilipin-3/TIP47 Antibody

Images

 
Western Blot: TIP47 Antibody [NB110-40764] - Detection of TIP47 in 3T3 L1 lysate.
Immunocytochemistry/ Immunofluorescence: TIP47 Antibody [NB110-40764] - TIP47 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, PmSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.00 mg/ml

Perilipin-3/TIP47 Antibody Summary

Immunogen
A synthetic peptide made to a region within the C-terminus (within residues 350-435) of the human TIP47 protein. [Swiss-Prot# O60664]
Localization
Cytoplasm. Endosome. Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLIN3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
This TIP47 antibody is useful for Western Blot and Immunocytochemistry/Immunofluorescence. In Western Blot analysis, a band is seen at ~47 kDa, representing isoform B in both human (faint) and mouse lysates. In some samples, a ~28 kDa band may be observed which represents a splice isoform. In ICC/IF, endosomal staining was observed in U2OS cells.
Control
Perilipin-3/TIP47 Overexpression Lysate
Control Peptide
Publications
Read Publications using
NB110-40764 in the following applications:

Reactivity Notes

Human, mouse, primate and porcine.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.00 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Perilipin-3/TIP47 Antibody

  • Cargo selection protein TIP47
  • M6PRBP1
  • M6PRBP1MGC2012
  • Mannose-6-phosphate receptor-binding protein 1
  • perilipin 3,47 kDa MPR-binding protein
  • Perilipin3
  • Perilipin-3
  • Placental protein 17,47 kDa mannose 6-phosphate receptor-binding protein
  • PLIN3
  • PP17
  • PP17MGC11117
  • tail-interacting protein, 47 kD
  • TIP47
  • TIP47mannose-6-phosphate receptor binding protein 1

Background

TIP47 (tail-interacting protein 47) is a member of PAT family of proteins (perilipin, ADRP, S3-12 and OXPAT) which plays a key role in the packaging/storage of neutral lipids and is required for mannose 6-phosphate receptors (M6PR) transport from endosomes to trans-Golgi network (TGN). TIP47 localizes in cytoplasm, endosome membrane as peripheral membrane protein towards cytoplasmic side, core of lipid bodies/ lipid droplet, in lipid sails and in the envelope, and it interacts with M6PR, IGF2R etc. via the cytoplasmic domain. As a cargo protein, TIP47 facilitates the delivery of M6PR from endosomes to TGN where it binds to cytoplasmic domains of cation-independent/-dependent M6PR and translocates to late endosomes by binding to Rab9 GTPase. Like other PAT family members, TIP47 associates with lipid droplets and influence insulin signaling as evidenced by combined knockout of ADRP /TIP47 leading to decreased insulin sensitivity. During HIV-1 infection, TIP47 acts as a cellular cofactor playing a part in Env incorporation, allowing the encounter and physical association between HIV-1 Gag and Env proteins during viral assembly process. TIP47 is identical to pregnancy-related placental tissue protein 17b (PP17b) and is overexpressed in cervical carcinoma patients wherein it is considered a serum tumor marker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Perilipin-3/TIP47 Antibody (NB110-40764)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(5)
All Applications
Filter By Species
Human
(4)
Mouse
(4)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB110-40764 Applications Species
Kuo A, Lee MY, Sessa WC. Lipid Droplet Biogenesis and Function in the Endothelium. Circ. Res. 2017 Jan 24 [PMID: 28119423]
Covington JD, Noland RC, Hebert RC et al. Perilipin 3 Differentially Regulates Skeletal Muscle Lipid Oxidation in Active, Sedentary and Type 2 Diabetic Males. J. Clin. Endocrinol. Metab. 2015 Jul 14 [PMID: 26171795]
Covington Jd, Bajpeyi S, Moro C et al. Effects of exercise training in women with polycystic ovary syndrome on adipose expression of perilipin 3. Eur. J. Endocrinol. 2014 Oct 23 [PMID: 25342854] (WB, Human) WB Human
Covington JD, Galgani JE, Moro C et al. Skeletal Muscle Perilipin 3 and Coatomer Proteins Are Increased following Exercise and Are Associated with Fat Oxidation. PLoS ONE 3/17/2014 [PMID: 24632837] (WB, Human) WB Human
Wolins NE, Quaynor BK, Skinner JR et al. S3-12, Adipophilin, and TIP47 package lipid in adipocytes. J Biol Chem 280(19):19146-55. 2005 May 13. [PMID: 15731108] (ICC/IF, WB, Human, Mouse) ICC/IF, WB Human, Mouse
Covington JD. THE EFFECTS OF AEROBIC EXERCISE AND LIPOLYTIC STIMULATION ON SKELETAL MUSCLE AND ADIPOSE EXPRESSION OF PERILIPIN 3 (PLIN3) IN INDIVIDUALS WITH TYPE 2 DIABETES AND WOMEN WITH POLYCYSTIC OVARY SYNDROME. Thesis. 2017 (Human) Human
Helsley RN, Varadharajan V, Brown AL et al. Obesity-linked suppression of membrane-bound O-acyltransferase 7 (MBOAT7) drives non-alcoholic fatty liver disease Elife Oct 17 2019 [PMID: 31621579] (Mouse) Mouse
Keenan SN, Meex RC, Lo JCY et al. Perilipin 5 Deletion in Hepatocytes Remodels Lipid Metabolism and Causes Hepatic Insulin Resistance in Mice. Diabetes Jan 7 2019 [PMID: 30617219] (WB, Mouse) WB Mouse
Adam M, Heikela H, Sobolewski C, Portius D. Hydroxysteroid (17b) dehydrogenase 13 deficiency triggers hepatic steatosis and inflammation in mice. FASEB J. 2018 Jan 31 [PMID: 29401633] (WB, Mouse) WB Mouse
Xu M, Zeng Y, Chi D et al. The dynamic pattern of PLIN3 in pig oocytes and cumulus cells during in vitro maturation Zygote 2018 Feb 01 [PMID: 29233207] (Porcine) Porcine

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Product General Protocols

View specific protocols for Perilipin-3/TIP47 Antibody (NB110-40764): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Perilipin-3/TIP47 Antibody (NB110-40764) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-40764AF350
Alexa Fluor 405 NB110-40764AF405
Alexa Fluor 488 NB110-40764AF488
Alexa Fluor 532 NB110-40764AF532
Alexa Fluor 594 NB110-40764AF594
Alexa Fluor 647 NB110-40764AF647
Alexa Fluor 700 NB110-40764AF700
Alexa Fluor 750 NB110-40764AF750
Biotin NB110-40764B
DyLight 350 NB110-40764UV
DyLight 405 NB110-40764V
DyLight 488 NB110-40764G
DyLight 550 NB110-40764R
DyLight 594 NB110-40764DL594
DyLight 650 NB110-40764C
DyLight 680 NB110-40764FR
DyLight 755 NB110-40764IR
FITC NB110-40764F
HRP NB110-40764H
Janelia Fluor 549 NB110-40764JF549
Janelia Fluor 646 NB110-40764JF646

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