Western Blot: OXPAT Antibody [NB110-60509] - Detection of OXPAT in human (lane 1) and mouse (lane 2) heart lysate.
Immunohistochemistry: OXPAT Antibody [NB110-60509] - Staining of OXPAT in paraffin embedded mouse fat.
Simple Western: OXPAT Antibody [NB110-60509] - Simple Western lane view shows a specific band for OXPAT in 0.5 mg/ml of Human Heart (left) and Mouse Heart (right) lysate. This experiment was performed under reducing ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.1% Sodium Azide
Immunogen affinity purified
This OXPAT antibody is useful for Western blot and Immunohistochemistry on paraffin-embedded sections. In WB a band can be seen at ~50 kDa, representing the OXPAT protein. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24648545)In Simple Western only 10-15 uL of the recommended dilution is used per data point.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human, bovine and mouse. Immunogen has 94% homology to sheep and porcine and 84% homology to rat.
Alternate Names for OXPAT Antibody
lipid storage droplet protein 5
OXPAT (PLIN5/Perilipin 5) is a novel PAT-1 (Perilipin, ADRP and TIP47) that binds to lipid droplets (LDs) and protects them from lipolytic degradation. The tissue distribution of PAT-1 proteins differs considerably, however, all of them are involved in accumulation and metabolism of lipids in LDs. OXPAT covers LDs in the cytosol and its expression is highest in tissues with a high fat-oxidative capacity, such as heart, oxidative (type I) skeletal muscles and brown adipose tissue wherein the OXPAT contents parallel the LD content. OXPAT overexpression in OP-9 and COS-7 cells increased cellular triglyceride storage in parallel with increases in fatty acid oxidation and induction of gene expression of mitochondrial enzymes involved in oxidative metabolism. OXPAT has the potential of modulating the interaction of the major triacylglycerol lipase ATGL with LD as well as with its co-factor CGI-58 in a well control manner to regulate LD lipolysis. OXPAT expression is up-regulated by PPARalpha, and this regulation appears to be part of an expression program that shifts the metabolism of cells from fatty acid storage to oxidation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.