OXPAT Antibody

Images

 
Western Blot: OXPAT Antibody [NB110-60509] - Detection of OXPAT in human (lane 1) and mouse (lane 2) heart lysate.
Immunohistochemistry: OXPAT Antibody [NB110-60509] - Staining of OXPAT in paraffin embedded mouse fat.
Simple Western: OXPAT Antibody [NB110-60509] - Simple Western lane view shows a specific band for OXPAT in 0.5 mg/ml of Human Heart (left) and Mouse Heart (right) lysate. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, Bv, ShSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

OXPAT Antibody Summary

Immunogen
A synthetic peptide made to a region of the human OXPAT protein, within residues 300-400. [UniProt# Q00G26]
Predicted Species
Sheep (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLIN5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2.0 ug/ml
  • Simple Western 10 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1.0-5.0 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1.0-5.0 ug/ml
Application Notes
This OXPAT antibody is useful for Western blot and Immunohistochemistry on paraffin-embedded sections. In WB a band can be seen at approx. 50 kDa, representing the OXPAT protein. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24648545). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 26096040).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Publications
Read Publications using
NB110-60509 in the following applications:

Reactivity Notes

porcine reactivity reported in scientific literature (PMID: 29094660).Human, bovine and mouse. Immunogen has 94% homology to sheep and porcine and 84% homology to rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for OXPAT Antibody

  • lipid storage droplet protein 5
  • LSDA5
  • LSDP5
  • MLDP
  • OXPAT
  • perilipin 5
  • perilipin-5

Background

OXPAT (PLIN5/Perilipin 5) is a novel PAT-1 (Perilipin, ADRP and TIP47) that binds to lipid droplets (LDs) and protects them from lipolytic degradation. The tissue distribution of PAT-1 proteins differs considerably, however, all of them are involved in accumulation and metabolism of lipids in LDs. OXPAT covers LDs in the cytosol and its expression is highest in tissues with a high fat-oxidative capacity, such as heart, oxidative (type I) skeletal muscles and brown adipose tissue wherein the OXPAT contents parallel the LD content. OXPAT overexpression in OP-9 and COS-7 cells increased cellular triglyceride storage in parallel with increases in fatty acid oxidation and induction of gene expression of mitochondrial enzymes involved in oxidative metabolism. OXPAT has the potential of modulating the interaction of the major triacylglycerol lipase ATGL with LD as well as with its co-factor CGI-58 in a well control manner to regulate LD lipolysis. OXPAT expression is up-regulated by PPARalpha, and this regulation appears to be part of an expression program that shifts the metabolism of cells from fatty acid storage to oxidation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for OXPAT Antibody (NB110-60509)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 2 applications: IHC-Fr, WB.


Filter By Application
IHC-Fr
(1)
WB
(3)
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Human
(4)
Mouse
(2)
Porcine
(1)
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Showing Publications 1 - 9 of 9.
Publications using NB110-60509 Applications Species
Zhong W, Fan B, Cong H et al. Oleic acid-induced perilipin 5 expression and lipid droplets formation are regulated by the PI3K/PPAR alpha pathway in HepG2 cells Appl Physiol Nutr Metab Aug 1 2019 [PMID: 31274012]
Larsen S, Vigelso A, Dandanell S et al. Simvastatin-Induced Insulin Resistance May Be Linked to Decreased Lipid Uptake and Lipid Synthesis in Human Skeletal Muscle: the LIFESTAT Study. Journal of Diabetes Research Sep 12 2018 (WB, Human) WB Human
Larsen Steen, Vigelso Andreas, Dandanell Sune et al. Simvastatin-Induced Insulin Resistance May Be Linked to Decreased Lipid Uptake and Lipid Synthesis in Human Skeletal Muscle: the LIFESTAT Study. Journal of Diabetes Research 2018 Sep 12 [PMID: 30276217] (WB, Human) WB Human
Kristensen MD, Petersen SM, Moller KE et al. Obesity leads to impairments in the morphology and organization of human skeletal muscle lipid droplets and mitochondrial networks, which are resolved with gastric bypass surgery-induced improvements in insulin sensitivity Acta Physiol (Oxf) May 23 2018 [PMID: 29791782] (Human) Human
Lambertz J, Berger T, Mak TW et al. Lipocalin-2 in Fructose-Induced Fatty Liver Disease. Front Physiol 2017 Nov 28 [PMID: 29234288] (Mouse) Mouse
Zappaterra M, Mazzoni M, Zambonelli P, Davoli R. Investigation of the Perilipin 5 gene expression and association study of its sequence polymorphism with meat and carcass quality traits in different pig breeds animal. 2017 Nov 02 [PMID: 29094660] (Porcine) Porcine
Vigelso A, Gram M, Wiuff C et al. Effects of immobilization and aerobic training on proteins related to intramuscular substrate storage and metabolism in young and older men. Eur. J. Appl. Physiol. 2015 Dec 01 [PMID: 26626913] (WB, Human) WB Human
Revuelta-Lopez E, Cal R, Julve J et al. Hypoxia worsens the impact of intracellular triglyceride accumulation promoted by electronegative low-density lipoprotein in cardiomyocytes by impairing perilipin 5 upregulation. Int J Biochem Cell Biol 2015 Aug [PMID: 26096040]
Fahrenbach JP, Stoller D, Kim G et al. Abcc9 is required for the transition to oxidative metabolism in the newborn heart. FASEB J. 3/19/2014 [PMID: 24648545] (IHC-Fr, Mouse) IHC-Fr Mouse

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Product General Protocols

View specific protocols for OXPAT Antibody (NB110-60509): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for OXPAT Antibody (NB110-60509) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-60509AF350
Alexa Fluor 405 NB110-60509AF405
Alexa Fluor 488 NB110-60509AF488
Alexa Fluor 532 NB110-60509AF532
Alexa Fluor 594 NB110-60509AF594
Alexa Fluor 647 NB110-60509AF647
Alexa Fluor 700 NB110-60509AF700
Alexa Fluor 750 NB110-60509AF750
Biotin NB110-60509B
DyLight 350 NB110-60509UV
DyLight 405 NB110-60509V
DyLight 488 NB110-60509G
DyLight 550 NB110-60509R
DyLight 594 NB110-60509DL594
DyLight 650 NB110-60509C
DyLight 680 NB110-60509FR
DyLight 755 NB110-60509IR
FITC NB110-60509F
HRP NB110-60509H
Janelia Fluor 549 NB110-60509JF549
Janelia Fluor 646 NB110-60509JF646

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Array NB110-60509

Bioinformatics Tool for OXPAT Antibody (NB110-60509)

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Research Areas for OXPAT Antibody (NB110-60509)

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Blogs on OXPAT.

OXPAT: Regulating Lipid Metabolism during Dietary Fluctuations
OXPAT is the mammalian form of the PAT (perilipin, adipophilin, and TIP47) gene family that consists of proteins associated with lipid droplets (LDs) in fat-storing adipocyte cells. They serve to protect LDs from natural lipases responsible for breaki...  Read full blog post.

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Bioinformatics

Gene Symbol PLIN5
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