OXPAT Antibody - BSA Free

Images

 
Western Blot: OXPAT Antibody [NB110-60509] - Detection of OXPAT in human (lane 1) and mouse (lane 2) heart lysate.
Immunohistochemistry: OXPAT Antibody [NB110-60509] - Staining of OXPAT in paraffin embedded mouse fat.
Simple Western: OXPAT Antibody [NB110-60509] - Simple Western lane view shows a specific band for OXPAT in 0.5 mg/ml of Human Heart (left) and Mouse Heart (right) lysate. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, Bv, ShSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

OXPAT Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to a region of the human OXPAT protein, within residues 300-400. [UniProt# Q00G26]
Predicted Species
Sheep (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLIN5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 26096040)
  • Immunohistochemistry 1.0-5.0 ug/ml
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 24648545)
  • Immunohistochemistry-Paraffin 1.0-5.0 ug/ml
  • Simple Western 10 ug/ml
  • Western Blot 0.5-2.0 ug/ml
Application Notes
In WB a band can be seen at approx. 50 kDa, representing the OXPAT protein.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
OXPAT Peptide (NB110-60509PEP)
Publications
Read Publications using
NB110-60509 in the following applications:

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 29094660). Immunogen displays the following percentage of sequence identity for non-tested species: sheep (94%), rat (84%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for OXPAT Antibody - BSA Free

  • lipid storage droplet protein 5
  • LSDA5
  • LSDP5
  • MLDP
  • OXPAT
  • perilipin 5
  • perilipin-5

Background

OXPAT (PLIN5/Perilipin 5) is a novel PAT-1 (Perilipin, ADRP and TIP47) that binds to lipid droplets (LDs) and protects them from lipolytic degradation. The tissue distribution of PAT-1 proteins differs considerably, however, all of them are involved in accumulation and metabolism of lipids in LDs. OXPAT covers LDs in the cytosol and its expression is highest in tissues with a high fat-oxidative capacity, such as heart, oxidative (type I) skeletal muscles and brown adipose tissue wherein the OXPAT contents parallel the LD content. OXPAT overexpression in OP-9 and COS-7 cells increased cellular triglyceride storage in parallel with increases in fatty acid oxidation and induction of gene expression of mitochondrial enzymes involved in oxidative metabolism. OXPAT has the potential of modulating the interaction of the major triacylglycerol lipase ATGL with LD as well as with its co-factor CGI-58 in a well control manner to regulate LD lipolysis. OXPAT expression is up-regulated by PPARalpha, and this regulation appears to be part of an expression program that shifts the metabolism of cells from fatty acid storage to oxidation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OXPAT Antibody (NB110-60509)(16)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 6 applications: FLOW, ICC/IF, IHC, IHC-Fr, IP, WB.


Filter By Application
FLOW
(1)
ICC/IF
(1)
IHC
(1)
IHC-Fr
(1)
IP
(1)
WB
(6)
All Applications
Filter By Species
Human
(4)
Mouse
(7)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB110-60509 Applications Species
Gan X, Zhao J, Chen Y et al. Plin5 inhibits proliferation and migration of vascular smooth muscle cell through interacting with PGC-1alpha following vascular injury Bioengineered 2022-04-01 [PMID: 35470759] (WB, IP, Mouse) WB, IP Mouse
Fillmore N, Hou V, Sun J et al. Cardiac specific knock-down of peroxisome proliferator activated receptor alpha prevents fasting-induced cardiac lipid accumulation and reduces perilipin 2 PloS one 2022-03-08 [PMID: 35259201] (WB, Mouse) WB Mouse
Miao H, Li B, Wang Z et al. Lipidome Atlas of the Developing Heart Uncovers Dynamic Membrane Lipid Attributes Underlying Cardiac Structural and Metabolic Maturation Research 2022-01-01 (WB, Mouse)

Details:
Dilution used in WB 1:500
WB Mouse
Zhao Y, Albrecht E, Li Z et al. Distinct Roles of Perilipins in the Intramuscular Deposition of Lipids in Glutamine-Supplemented, Low-, and Normal-Birth-Weight Piglets Frontiers in Veterinary Science 2021-06-21 [PMID: 34235195] (IHC) IHC
Zhong W, Fan B, Cong H et al. Oleic acid-induced perilipin 5 expression and lipid droplets formation are regulated by the PI3K/PPAR alpha pathway in HepG2 cells Appl Physiol Nutr Metab 2019-08-01 [PMID: 31274012]
Larsen Steen, Vigelso Andreas, Dandanell Sune et al. Simvastatin-Induced Insulin Resistance May Be Linked to Decreased Lipid Uptake and Lipid Synthesis in Human Skeletal Muscle: the LIFESTAT Study. J Diabetes Res. 2018-09-12 [PMID: 30276217] (WB, Human) WB Human
Kristensen MD, Petersen SM, Moller KE et al. Obesity leads to impairments in the morphology and organization of human skeletal muscle lipid droplets and mitochondrial networks, which are resolved with gastric bypass surgery-induced improvements in insulin sensitivity Acta Physiol (Oxf) 2018-05-23 [PMID: 29791782] (Human) Human
Lambertz J, Berger T, Mak TW et al. Lipocalin-2 in Fructose-Induced Fatty Liver Disease. Front Physiol 2017-11-28 [PMID: 29234288] (Mouse) Mouse
Zappaterra M, Mazzoni M, Zambonelli P, Davoli R. Investigation of the Perilipin 5 gene expression and association study of its sequence polymorphism with meat and carcass quality traits in different pig breeds animal. 2017-11-02 [PMID: 29094660] (Porcine) Porcine
Vigelso A, Gram M, Wiuff C et al. Effects of immobilization and aerobic training on proteins related to intramuscular substrate storage and metabolism in young and older men. Eur. J. Appl. Physiol. 2015-12-01 [PMID: 26626913] (WB, Human) WB Human
Show All 16 Publications.

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Product General Protocols

View specific protocols for OXPAT Antibody (NB110-60509): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional OXPAT Products

Array NB110-60509

Research Areas for OXPAT Antibody (NB110-60509)

Find related products by research area.

Blogs on OXPAT.

OXPAT: Regulating Lipid Metabolism during Dietary Fluctuations
OXPAT is the mammalian form of the PAT (perilipin, adipophilin, and TIP47) gene family that consists of proteins associated with lipid droplets (LDs) in fat-storing adipocyte cells. They serve to protect LDs from natural lipases responsible for breaki...  Read full blog post.

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Bioinformatics

Gene Symbol PLIN5