Hormone-sensitive Lipase/HSL Antibody

Images

 
Western Blot: HSL Antibody [NB110-37253] - Detection of HSL using NB110-37253. Lane 1: Liver extract (negative control) Lane 2: Fat extract (10ug) (positive sample).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price

Hormone-sensitive Lipase/HSL Antibody Summary

Immunogen
A fusion protein containing an internal portion of the rat HSL protein (between residues 699-908). [Swiss-Prot P15304]
Localization
Cytoplasmic
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIPE
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This HSL antibody is useful for Western Blot, where a band at ~84kDa is seen representing HSL protein. A non-specific band may be seen at ~89 kDa. Bands at ~102, 113, and 127 kDa may also be seen in the testis of sexually immature rats.
Positive Control
Adipose Lysate (NB820-59170)
Publications
Read Publications using
NB110-37253 in the following applications:

  • WB
    3 publications

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS containing 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Purity
IgG purified

Alternate Names for Hormone-sensitive Lipase/HSL Antibody

  • hormone-sensitive lipase testicular isoform
  • Hormonesensitive Lipase
  • Hormone-sensitive Lipase
  • HSL
  • HSLEC 3.1.1.79
  • LHS
  • lipase, hormone-sensitive
  • LIPE

Background

HSL (hormone-sensitive lipase) is a key enzyme in lipid metabolism regulation in several tissues and this intracellular neutral lipase hydrolyzes triacylglycerols (TG), diacylglycerols (DG), and monoacylglycerols, as well as cholesterol and retinyl esters. While ATGL selectively performs the first step in TG hydrolysis, HSL is the most active enzyme against DG, which gets hydrolyzed faster than TG. HSL has been shown to express in several non-adipose tissues, including ovary, adrenal gland, heart, skeletal and smooth muscle, placenta, and pancreatic beta-cells, as well as testes. Under basal conditions, HSL is exclusively found in the cytosol of adipocytes, whereas upon phosphorylation mediated activation via PKA, HSL translocates from cytosol to lipid droplet surfaces for participation in lipolysis. HSL activity is regulated primarily by serine phosphorylation, which is stimulatory at Ser-563/Ser-659 via PKA and inhibitory at Ser-565 via AMPK. Mice lacking HSL have decreased nonesterified fatty acid (NEFA) and increased cholesterol concentrations in plasma and are resistant to diet-induced obesity. HSL has emerged as a potential target for lipid disorders and obesity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Hormone-sensitive Lipase/HSL Antibody (NB110-37253)(3)

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 488 Labeled NB110-37253G

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Diseases for Hormone-sensitive Lipase/HSL Antibody (NB110-37253)

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Bioinformatics

Gene Symbol LIPE
Entrez