Perilipin Antibody

Images

 
Western Blot: Perilipin Antibody [NB110-40760] - Total protein from Human breast (lane 1), Human adipose membrane fraction (lane 2) and Human adipose tissue (lane 3) were separated on a 12% gel by SDS-PAGE. Protein was ...read more
Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760] - IHC analysis of formalin fixed paraffin embedded section of liver tissue from mouse with Perilipin antibody at 1:200 dilution. The hepatocytes developed ...read more
Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760] - IHC analysis of formalin fixed paraffin embedded section of fat tissue from mouse with Perilipin antibody at 1:200 dilution. The antibody generated an ...read more
Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760] - IHC analysis of formalin fixed paraffin embedded section of liver tissue from mouse with Perilipin antibody at 1:200 dilution. The hepatocytes developed ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.1 mg/ml

Perilipin Antibody Summary

Immunogen
A synthetic peptide made to a region between residues 450-522 (C-terminus) of the human Perilipin protein. [Swiss-Prot# O60240]
Localization
Endoplasmic reticulum, Lipid droplet (surface-associated).
Predicted Species
Sheep (92%), Bovine (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLIN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This Perilipin antibody is useful for Western blot anlaysis where a band at ~60 kDa is seen. Immunocytochemistry/Immunofluorescence was reported in scientific literature.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Liver Lysate (NB820-59232)
Control Peptide
Perilipin Peptide (NB110-40760PEP)
Publications
Read Publications using
NB110-40760 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Perilipin Antibody

  • PERI
  • perilipin 1
  • Perilipin
  • perilipin-1
  • PLIN
  • PLIN1
  • PLINLipid droplet-associated protein

Background

Perilipin is a member of the perilipin or PAT family which includes perilipin or perilipin 1 (PLIN1), ADRP/perilipin 2, TIP47/perilipin 3, S3-12/perilipin 4, and LSDP5/perilipin 5 (also called OXPAT). PLIN1 is localized on lipid droplet surfaces in adipocytes and steroidogenic cells wherein it plays a key role in basal/stimulated lipid storage as well as breakdown. Under basal conditions, perilipin protects the lipid core from lipases (e.g. hormone-sensitive lipase and adipocyte TAG lipase) and co-activator CGI-58 by sequestering the enzymes from the lipid droplet surfaces. However, upon beta-adrenergic stimulation, PKA phosphorylates PLIN1 and causes the release of CGI-58 so it can bind and stimulate ATGL and also allows/recruits HSL to translocate to the lipid droplet surface. PLIN1 knockout increases basal lipolysis and decreases LD size in adipocytes and causes resistance to diet induced obesity in mice. In humans, lower PLIN1 expression is associated with higher rates of lipolysis and mutation in PLIN1 or its expression in obese human adipose tissue, correlates positively with insulin sensitivity, and have been established as the cause of familial partial lipodystrophy type 4 (FPLD4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Perilipin Antibody (NB110-40760)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(2)
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Filter By Species
Human
(1)
Mouse
(2)
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Product General Protocols

View specific protocols for Perilipin Antibody (NB110-40760): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Perilipin Antibody (NB110-40760) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-40760AF405
Alexa Fluor 488 Labeled NB110-40760AF488
Alexa Fluor 647 Labeled NB110-40760AF647
Alexa Fluor 700 Labeled NB110-40760AF700
Biotin Labeled NB110-40760B
DyLight 405 Labeled NB110-40760V
DyLight 488 Labeled NB110-40760G
DyLight 550 Labeled NB110-40760R
DyLight 650 Labeled NB110-40760C
FITC Labeled NB110-40760F
HRP Labeled NB110-40760H

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Bioinformatics Tool for Perilipin Antibody (NB110-40760)

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Bioinformatics

Gene Symbol PLIN1
Entrez