SREBP1 Antibody (2A4)


Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Western blot analysis of SREBP1 expression in HeLa lysate using NB600-582.
Simple Western: SREBP1 Antibody (2A4) [NB600-582] - Simple Western lane view shows a specific band for SREBP1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa more

Product Details

Reactivity Hu, Mu, Rt, Ca, Ch, SyHa, HaSpecies Glossary
Applications WB, Simple Western
1 mg/ml

SREBP1 Antibody (2A4) Summary

6 His-tag fusion protein of human SREBP1 corresponding to amino acids 301-407. [UniProt# P36956]
Cytoplasmic and nuclear
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
1 mg/ml
Protein G purified


Application Notes
This SREBP1 (clone 2A4) antibody is useful for Western blot where a band can be seen at 125 kDa (precursor) and additional bands may be seen at 60-70 kDa (cleaved).In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Read Publications using
NB600-582 in the following applications:

  • WB
    19 publications

Reactivity Notes

Human, mouse, rat and Golden Syrian Hamster. Canine reactivity reported in scientific literature (PMID: 23720350).Hamster reactivity reported in scientific literature (PMID: 24393244). Chicken reactivity reported in multiple pieces scientific literature

Alternate Names for SREBP1 Antibody (2A4)

  • BHLHD1
  • Class D basic helix-loop-helix protein 1
  • SREBP 1c
  • SREBP-1
  • sterol regulatory element binding protein-1
  • sterol regulatory element binding transcription factor 1
  • sterol regulatory element-binding protein 1
  • Sterol regulatory element-binding transcription factor 1


SREBP1 (sterol regulatory element binding protein 1, also called SREBF1 or BHLHD1) is a bHLH-LZ (basic helix-loop-helix-leucine zipper) transcription factor that acts as a key lipogenic transcription factor controlled by glucose/insulin and preferentially regulates lipogenesis via activating genes involved in fatty acid as well as triglyceride synthesis. It is a transcriptional activator required for lipid homeostasis and regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. SREBP1 has a dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (sterol regulatory element 1; 5'-ATCACCCCAC-3'). It exists as multi-pass membrane protein in the membranes of ER, Golgi apparatus and cytoplasmic vesicle /COPII-coated vesicles and moves from ER to Golgi in the absence of sterols. It forms a tight complex with SCAP in ER membranes and interacts with LMNA. AMPK phosphorylate SREBP1 leading to suppression of protein processing and nuclear translocation, and target gene expression repression. Moreover, SIK1 mediated phosphorylation at Ser-402 site represses its activity through inhibition of DNA-binding.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Po, Ch, Ha, Pl, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Ch
Applications: WB, ELISA, GS, ICC/IF, IP
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, Flow, GS, IHC, IHC-P, IP
Species: Hu
Applications: WB, PEP-ELISA
Species: Hu, Rt, Po, Bv
Applications: WB, EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, RIA
Species: Hu, Mu, Po, Pm
Applications: WB, Simple Western
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC, ICFlow
Species: Hu
Applications: WB, IHC
Species: Hu, Mu
Applications: WB, ICC
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ChIP, IB, ICC/IF, IHC, IHC-P, IP

Publications for SREBP1 Antibody (NB600-582)(23)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Canine, Chicken, Hamster.

We have publications tested in 1 application: WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NB600-582 Applications Species
Dungan CM, Li J, Williamson DL. Caloric Restriction Normalizes Obesity-Induced Alterations on Regulators of Skeletal Muscle Growth Signaling. Lipids Jun 11 2016 12:00AM [PMID:27289530] (WB, Mouse) WB Mouse
Dungan CM, Li J, Williamson DL. Caloric Restriction Normalizes Obesity-Induced Alterations on Regulators of Skeletal Muscle Growth Signaling. Lipids 2016 Jun 11 [PMID:27289530] (WB, Mouse) WB Mouse
Mahmood S, Birkaya B, Rideout TC, Patel MS. Lack of Mitochondria-generated acetyl-CoA by pyruvate dehydrogenase complex downregulates gene expression in hepatic de novo lipogenic pathway. Am. J. Physiol. Endocrinol. Metab. May 10 2016 12:00AM [PMID:27166281] (WB, Mouse) WB Mouse
Casali BT, Corona AW, Mariani MM et al. Omega-3 Fatty Acids Augment the Actions of Nuclear Receptor Agonists in a Mouse Model of Alzheimer's Disease J. Neurosci. 2015 Jun 17 [PMID:26085639] (WB, Mouse) WB Mouse
Dungan CM, Li Z, Wright DC, Williamson DL. Hyperactive mTORC1 signaling is unaffected by metformin treatment in aged skeletal muscle. Muscle Nerve 2015 Apr 29 [PMID:25926238]
Li Z, Dungan Cm, Carrier B et al. Alpha-Lipoic Acid Supplementation Reduces mTORC1 Signaling in Skeletal Muscle from High Fat Fed, Obese Zucker Rats Lipids et al. 2014 Dec 01 [PMID:25366515] (WB, Rat) WB Rat
Rideout TC, Ramprasath V, Griffin JD et al. Phytosterols protect against diet-induced hypertriglyceridemia in Syrian golden hamsters. Lipids Health Dis 2014 Jan 6 [PMID:24393244] (WB, Hamster) WB Hamster
Wen S, Jadhav KS, Williamson DL, Rideout TC. Treadmill Exercise Training Modulates Hepatic Cholesterol Metabolism and Circulating PCSK9 Concentration in High-Fat-Fed Mice. J Lipids 2013 [PMID:23862065] (WB, Mouse) WB Mouse
Pajvani UB, Qiang L, Kangsamaksin T et al. Inhibition of Notch uncouples Akt activation from hepatic lipid accumulation by decreasing mTorc1 stability. Nat Med 2013 Aug [PMID:23832089] (WB, Mouse) WB Mouse
Raksaseri P, Chatsudthipong V, Muanprasat C, Soodvilai S. Activation of liver X receptors reduces cystic fibrosis transmembrane conductance regulator (CFTR)-mediated chloride transport in kidney collecting duct cells. Am J Physiol Renal Physiol 2013 May 29 [PMID:23720350] (WB, Canine) WB Canine
Show All 23 Publications.

Reviews for SREBP1 Antibody (NB600-582) (0)

There are no reviews for SREBP1 Antibody (NB600-582). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

View specific protocols for SREBP1 Antibody (NB600-582): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SREBP1 Antibody (NB600-582). (Showing 1 - 2 of 2 FAQs).

  1. I would like to order SREBP-1 antibody, catalog number NB600-582. I am wondering if it might cross react with MDCK-C cells from dog?
    • We have not tested NB600-582 with any dog samples, and cannot guarantee that it will cross-react. However, if you are interested in trying this antibody with dog samples you would be eligible for our Innovators Reward Program.
  2. I am looking for SREBF1 antibodies that can cross-react with Zebrafish srebf1. I am wondering whether you have any SREBF1 antibodies that have been tested for zebra fish or have high sequence identity in the immunogen region?
    • Unfortunately we do not have any antibodies that have been tested in zebra fish. I have included the immunogen sequence for the antibodies it might be worth considering. I am not sure exactly what the zebra fish sequence is, I was unable to find it on, so I was unable to check the homology. The following antibodies all have available sample sizes. If you decide to try one you would qualify for our Innovator's Reward Program. NB600-582: 301-407 aa, NB100-2215: 300-400 aa, NB100-60545: 700-800 aa

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB600-582AF405
Alexa Fluor (R) 488 Labeled NB600-582AF488
Alexa Fluor (R) 647 Labeled NB600-582AF647
Alexa Fluor (R) 700 Labeled NB600-582AF700
Biotin Labeled NB600-582B
DyLight 405 Labeled NB600-582V
DyLight 488 Labeled NB600-582G
DyLight 550 Labeled NB600-582R
DyLight 650 Labeled NB600-582C
FITC Labeled NB600-582F
HRP Labeled NB600-582H

Additional SREBP1 Antibody (2A4) Products

Related Products by Gene

Bioinformatics Tool for SREBP1 Antibody (NB600-582)

Discover related pathways, diseases and genes to SREBP1 Antibody (NB600-582). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SREBP1 Antibody (NB600-582)

Discover more about diseases related to SREBP1 Antibody (NB600-582).

Pathways for SREBP1 Antibody (NB600-582)

View related products by pathway.

PTMs for SREBP1 Antibody (NB600-582)

Learn more about PTMs related to SREBP1 Antibody (NB600-582).

Research Areas for SREBP1 Antibody (NB600-582)

Find related products by research area.

Blogs on SREBP1.

SREBP2 - regulating cholesterol homeostasis and lipid metabolism
Sterol regulatory element-binding proteins (SREBP) are important transcription factors regulating the synthesis and uptake of lipids including cholesterol. This essential role in lipid metabolism makes investigations into the functions SREBPs im...  Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g....  Read full blog post.

Contact Information

Product PDFs


Gene Symbol SREBF1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB600-582 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought