SREBP1 Antibody (2A4)

Images

 
Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Western blot analysis of whole cell lysate from HeLa cells showing a single specific band for the expression of SREBP1 precursor protein (~120 kDa).
Simple Western: SREBP1 Antibody (2A4) [NB600-582] - Simple Western lane view shows a specific band for SREBP1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Ch, Ha, SyHaSpecies Glossary
Applications WB, Simple Western, IHC
Clone
2A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

SREBP1 Antibody (2A4) Summary

Immunogen
6 His-tag fusion protein of human SREBP1 corresponding to amino acids 301-407. [UniProt# P36956]
Localization
Cytoplasmic and nuclear
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SREBF1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2ug/ml
  • Simple Western 1:12.5
  • Immunohistochemistry
Application Notes
This SREBP1 (clone 2A4) antibody is useful for Western blot where a band can be seen at 125 kDa (precursor) and additional bands may be seen at 60-70 kDa (cleaved).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
HeLa Lysate (NB800-PC9)
Publications
Read Publications using
NB600-582 in the following applications:

  • WB
    21 publications

Reactivity Notes

Human, mouse, rat and Golden Syrian Hamster. Canine reactivity reported in scientific literature (PMID: 23720350).Hamster reactivity reported in scientific literature (PMID: 24393244). Chicken reactivity reported in multiple pieces scientific literature

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for SREBP1 Antibody (2A4)

  • BHLHD1
  • Class D basic helix-loop-helix protein 1
  • SREBP 1c
  • SREBP-1
  • SREBP1bHLHd1SREBP-1c
  • sterol regulatory element binding protein-1
  • sterol regulatory element binding transcription factor 1
  • sterol regulatory element-binding protein 1
  • Sterol regulatory element-binding transcription factor 1

Background

SREBP1 (sterol regulatory element binding protein 1, also called SREBF1 or BHLHD1) is a bHLH-LZ (basic helix-loop-helix-leucine zipper) transcription factor that acts as a key lipogenic transcription factor controlled by glucose/insulin and preferentially regulates lipogenesis via activating genes involved in fatty acid as well as triglyceride synthesis. It is a transcriptional activator required for lipid homeostasis and regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. SREBP1 has a dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (sterol regulatory element 1; 5'-ATCACCCCAC-3'). It exists as multi-pass membrane protein in the membranes of ER, Golgi apparatus and cytoplasmic vesicle /COPII-coated vesicles and moves from ER to Golgi in the absence of sterols. It forms a tight complex with SCAP in ER membranes and interacts with LMNA. AMPK phosphorylate SREBP1 leading to suppression of protein processing and nuclear translocation, and target gene expression repression. Moreover, SIK1 mediated phosphorylation at Ser-402 site represses its activity through inhibition of DNA-binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, IHC

Publications for SREBP1 Antibody (NB600-582)(27)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Canine, Chicken, Hamster.

We have publications tested in 1 application: WB.


Filter By Application
WB
(21)
All Applications
Filter By Species
Human
(2)
Mouse
(11)
Rat
(4)
Canine
(1)
Chicken
(2)
Hamster
(2)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using NB600-582 Applications Species
Ikenoue T, Terakado Y, Zhu C, Liu X. Establishment and analysis of a novel mouse line carrying a conditional knockin allele of a cancer-specific FBXW7 mutation. Sci Rep. 2018 Jan 31 [PMID: 29386660] (WB, Mouse) WB Mouse
Khound R, Shen J, Song Y et al. Phytoceuticals in Fenugreek Ameliorate VLDL Overproduction and Insulin Resistance via the Insig Signaling Pathway. Mol Nutr Food Res 2017 Dec 06 [PMID: 29210166] (Mouse) Mouse
Ahmad F, Patrick S, Sheikh T et al. TERT-EZH2 network regulates lipid metabolism and DNA damage responses in glioblastoma J. Neurochem. 2017 Aug 21 [PMID: 28833137] (WB, Mouse) WB Mouse
Janssens S, Ciapaite J, Wolters JC et al. An In Vivo Magnetic Resonance Spectroscopy Study of the Effects of Caloric and Non-Caloric Sweeteners on Liver Lipid Metabolism in Rats. Nutrients. 2017 May 10 [PMID: 28489050] (WB, Rat) WB Rat
Dungan CM, Li J, Williamson DL. Caloric Restriction Normalizes Obesity-Induced Alterations on Regulators of Skeletal Muscle Growth Signaling. Lipids Jun 11 2016 [PMID: 27289530] (WB, Mouse) WB Mouse
Mahmood S, Birkaya B, Rideout TC, Patel MS. Lack of Mitochondria-generated acetyl-CoA by pyruvate dehydrogenase complex downregulates gene expression in hepatic de novo lipogenic pathway. Am. J. Physiol. Endocrinol. Metab. May 10 2016 [PMID: 27166281] (WB, Mouse) WB Mouse
Goedeke L, Rotllan N, Canfran-Duque A et al. MicroRNA-148a regulates LDL receptor and ABCA1 expression to control circulating lipoprotein levels. Nat Med 2015 Nov [PMID: 26437365]
Casali BT, Corona AW, Mariani MM et al. Omega-3 Fatty Acids Augment the Actions of Nuclear Receptor Agonists in a Mouse Model of Alzheimer's Disease J. Neurosci. 2015 Jun 17 [PMID: 26085639] (WB, Mouse) WB Mouse
Dungan CM, Li Z, Wright DC, Williamson DL. Hyperactive mTORC1 signaling is unaffected by metformin treatment in aged skeletal muscle. Muscle Nerve 2015 Apr 29 [PMID: 25926238]
Li Z, Dungan Cm, Carrier B et al. Alpha-Lipoic Acid Supplementation Reduces mTORC1 Signaling in Skeletal Muscle from High Fat Fed, Obese Zucker Rats Lipids et al. 2014 Dec 01 [PMID: 25366515] (WB, Rat)

Details:
SREBP1 / SREBP1c antibody used for WB in nuclear and cytoplasmic fractions of skeletal muscles from High Fat Fed, Obese Zucker Rats supplemented or not with Alpha-Lipoic Acid (Fig. 3c and d)
WB Rat
Show All 27 Publications.

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Product General Protocols

View specific protocols for SREBP1 Antibody (NB600-582): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SREBP1 Antibody (NB600-582). (Showing 1 - 2 of 2 FAQs).

  1. I would like to order SREBP-1 antibody, catalog number NB600-582. I am wondering if it might cross react with MDCK-C cells from dog?
    • We have not tested NB600-582 with any dog samples, and cannot guarantee that it will cross-react. However, if you are interested in trying this antibody with dog samples you would be eligible for our Innovators Reward Program.
  2. I am looking for SREBF1 antibodies that can cross-react with Zebrafish srebf1. I am wondering whether you have any SREBF1 antibodies that have been tested for zebra fish or have high sequence identity in the immunogen region?
    • Unfortunately we do not have any antibodies that have been tested in zebra fish. I have included the immunogen sequence for the antibodies it might be worth considering. I am not sure exactly what the zebra fish sequence is, I was unable to find it on uniprot.org, so I was unable to check the homology. The following antibodies all have available sample sizes. If you decide to try one you would qualify for our Innovator's Reward Program. NB600-582: 301-407 aa, NB100-2215: 300-400 aa, NB100-60545: 700-800 aa

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-582AF405
Alexa Fluor 488 Labeled NB600-582AF488
Alexa Fluor 647 Labeled NB600-582AF647
Alexa Fluor 700 Labeled NB600-582AF700
Biotin Labeled NB600-582B
DyLight 405 Labeled NB600-582V
DyLight 488 Labeled NB600-582G
DyLight 550 Labeled NB600-582R
DyLight 650 Labeled NB600-582C
HRP Labeled NB600-582H

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Blogs on SREBP1.

SREBP2 - regulating cholesterol homeostasis and lipid metabolism
Sterol regulatory element-binding proteins (SREBP) are important transcription factors regulating the synthesis and uptake of lipids including cholesterol. This essential role in lipid metabolism makes investigations into the functions SREBPs im...  Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g....  Read full blog post.

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Bioinformatics

Gene Symbol SREBF1
Entrez
Uniprot