Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Hepatic SREBP1c and SREBP2 protein abundance in C57BL/6 mice assigned to a high-fat (HF) or a high-fat/exercise (HF + EX) group for 8 weeks. Cytosolic and nuclear ...read more
Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Analysis of whole cell lysate from HeLa cells showing a single specific band for the expression of SREBP1 precursor protein (~120 kDa).
Simple Western: SREBP1 Antibody (2A4) [NB600-582] - Lane view shows a specific band for SREBP1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Genetic Strategies: Knockout Validated: SREBP1 Antibody (2A4) [NB600-582] - KO and OE of SREBP1 influenced lipid metabolism in Bel-7402 cells. The protein level of lipid-associated genes in SREBP1-KO, SREBP1-OE, ...read more
This SREBP1 (clone 2A4) antibody is useful for WB where a band can be seen at 125 kDa (precursor) and additional bands may be seen at 60-70 kDa (cleaved).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The use of this antibody in IHC paraffin embedded tissue has been questionable.
Canine reactivity reported in scientific literature (PMID: 23720350). Hamster reactivity reported in scientific literature (PMID: 24393244). Chicken reactivity reported in multiple pieces scientific literature. Monkey reactivity reported in scientific literature (PMID: 26437365).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Alternate Names for SREBP1 Antibody (2A4) - BSA Free
Class D basic helix-loop-helix protein 1
sterol regulatory element binding protein-1
sterol regulatory element binding transcription factor 1
SREBP1 (sterol regulatory element binding protein 1, also called SREBF1 or BHLHD1) is a bHLH-LZ (basic helix-loop-helix-leucine zipper) transcription factor that acts as a key lipogenic transcription factor controlled by glucose/insulin and preferentially regulates lipogenesis via activating genes involved in fatty acid as well as triglyceride synthesis. It is a transcriptional activator required for lipid homeostasis and regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. SREBP1 has a dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (sterol regulatory element 1; 5'-ATCACCCCAC-3'). It exists as multi-pass membrane protein in the membranes of ER, Golgi apparatus and cytoplasmic vesicle /COPII-coated vesicles and moves from ER to Golgi in the absence of sterols. It forms a tight complex with SCAP in ER membranes and interacts with LMNA. AMPK phosphorylate SREBP1 leading to suppression of protein processing and nuclear translocation, and target gene expression repression. Moreover, SIK1 mediated phosphorylation at Ser-402 site represses its activity through inhibition of DNA-binding.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Chen YJ, Lo YH, Chen YT et al. Magnesium lithospermate B improves metabolic changes in high-fat diet-fed rats with metabolic syndrome Journal of Functional Foods. 2015 Apr 01 (WB, Rat)
Details: SREBP1 antibody was used for WB application on lysates of liver tissue from rats that were fed different diet regimns as - normal diet control/NC, high-fat diet control/HC or HC+ MLB/Magnesium lithospermate B (MLB5-20/HC+5-20?mg/kg/day of MLB). Primary antibody was used at 1:1000 dilution in 5% skim milk and detected with HRP-conjugated secondary antibody - chemiluminescent HRP substrate (data shown in Fig. 4A).
FAQs for SREBP1 Antibody (NB600-582). (Showing 1 - 2 of 2 FAQs).
I would like to order SREBP-1 antibody, catalog number NB600-582. I am wondering if it might cross react with MDCK-C cells from dog?
We have not tested NB600-582 with any dog samples, and cannot guarantee that it will cross-react. However, if you are interested in trying this antibody with dog samples you would be eligible for our Innovators Reward Program.
I am looking for SREBF1 antibodies that can cross-react with Zebrafish srebf1. I am wondering whether you have any SREBF1 antibodies that have been tested for zebra fish or have high sequence identity in the immunogen region?
Unfortunately we do not have any antibodies that have been tested in zebra fish. I have included the immunogen sequence for the antibodies it might be worth considering. I am not sure exactly what the zebra fish sequence is, I was unable to find it on uniprot.org, so I was unable to check the homology. The following antibodies all have available sample sizes. If you decide to try one you would qualify for our Innovator's Reward Program. NB600-582: 301-407 aa, NB100-2215: 300-400 aa, NB100-60545: 700-800 aa
SREBP: Gatekeeper of Cholesterol Homeostasis SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. ... Read full blog post.
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