SREBP1 Antibody (2A4)


Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Hepatic SREBP1c and SREBP2 protein abundance in C57BL/6 mice assigned to a high-fat (HF) or a high-fat/exercise (HF + EX) group for 8 weeks. Cytosolic and nuclear more
Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Analysis of whole cell lysate from HeLa cells showing a single specific band for the expression of SREBP1 precursor protein (~120 kDa).
Simple Western: SREBP1 Antibody (2A4) [NB600-582] - Lane view shows a specific band for SREBP1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Genetic Strategies: Knockout Validated: SREBP1 Antibody (2A4) [NB600-582] - KO and OE of SREBP1 influenced lipid metabolism in Bel-7402 cells. The protein level of lipid-associated genes in SREBP1-KO, SREBP1-OE, more

Product Details

Reactivity Hu, Mu, Rt, Ca, Ch, Ha, Pm, SyHaSpecies Glossary
Applications WB, Simple Western, IHC, KO
1 mg/ml
Validated by:

Genetic Strategies


SREBP1 Antibody (2A4) Summary

6 His-tag fusion protein of human SREBP1 corresponding to amino acids 301-407. [UniProt# P36956]
Cytoplasmic and nuclear
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1-2ug/ml
  • Simple Western 1:12.5
  • Immunohistochemistry
  • Knockout Validated
Application Notes
This SREBP1 (clone 2A4) antibody is useful for WB where a band can be seen at 125 kDa (precursor) and additional bands may be seen at 60-70 kDa (cleaved).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
HeLa Nuclear Cell Lysate
Read Publications using
NB600-582 in the following applications:

  • 1 publication
  • WB
    31 publications

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 23720350). Hamster reactivity reported in scientific literature (PMID: 24393244). Chicken reactivity reported in multiple pieces scientific literature. Monkey reactivity reported in scientific literature (PMID: 26437365).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1 mg/ml
Protein G purified

Alternate Names for SREBP1 Antibody (2A4)

  • BHLHD1
  • Class D basic helix-loop-helix protein 1
  • SREBP 1c
  • SREBP-1
  • sterol regulatory element binding protein-1
  • sterol regulatory element binding transcription factor 1
  • sterol regulatory element-binding protein 1
  • Sterol regulatory element-binding transcription factor 1


SREBP1 (sterol regulatory element binding protein 1, also called SREBF1 or BHLHD1) is a bHLH-LZ (basic helix-loop-helix-leucine zipper) transcription factor that acts as a key lipogenic transcription factor controlled by glucose/insulin and preferentially regulates lipogenesis via activating genes involved in fatty acid as well as triglyceride synthesis. It is a transcriptional activator required for lipid homeostasis and regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. SREBP1 has a dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (sterol regulatory element 1; 5'-ATCACCCCAC-3'). It exists as multi-pass membrane protein in the membranes of ER, Golgi apparatus and cytoplasmic vesicle /COPII-coated vesicles and moves from ER to Golgi in the absence of sterols. It forms a tight complex with SCAP in ER membranes and interacts with LMNA. AMPK phosphorylate SREBP1 leading to suppression of protein processing and nuclear translocation, and target gene expression repression. Moreover, SIK1 mediated phosphorylation at Ser-402 site represses its activity through inhibition of DNA-binding.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu
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Applications: WB, ChIP, GS, ICC/IF, IHC, IHC-P, IP, KO
Species: Hu, Mu, Rt, Ca, Ch, Ha, Pm, SyHa
Applications: WB, Simple Western, IHC, KO

Publications for SREBP1 Antibody (NB600-582)(38)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Canine, Chicken, Hamster.

We have publications tested in 2 applications: IHC-F, WB.

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Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using NB600-582 Applications Species
Park Y, An M, Kim J, Lim Y Antiobesity effect of ethanolic extract of Ramulus mori in differentiated 3T3-L1 adipocytes and high-fat diet-induced obese mice Journal of Ethnopharmacology Jan 1 2020 [PMID: 31904494] (WB, Mouse) WB Mouse
Inhibition of Hepatotoxicity by a LXR Inverse Agonist in a Model of Alcoholic Liver Disease Sengupta M, Griffett K, Flaveny C et al. ACS Pharmacol Transl Sci [PMID: 31696159] (WB, IHC-F, Mouse) WB, IHC-F Mouse
Lin R, Jia Y, Wu F et al. Combined Exposure to Fructose and Bisphenol A Exacerbates Abnormal Lipid Metabolism in Liver of Developmental Male Rats Int J Environ Res Public Health 2019 Oct 28 [PMID: 31661889] (WB, Rat) WB Rat
Gouw AM, Margulis K, Liu NS et al. The MYC Oncogene Cooperates with Sterol-Regulated Element-Binding Protein to Regulate Lipogenesis Essential for Neoplastic Growth Cell Metab. Aug 19 2019 [PMID: 31447321]
Yang N, Li C, Li H et al. Emodin Induced SREBP1-Dependent and SREBP1-Independent Apoptosis in Hepatocellular Carcinoma Cells Front Pharmacol Jun 25 2019 [PMID: 31297058] (WB, Human) WB Human
Pham DD, Bruelle C, Thi Do H et al. Caspase-2 and p75 neurotrophin receptor (p75NTR) are involved in the regulation of SREBP and lipid genes in hepatocyte cells Cell Death Dis Jul 11 2019 [PMID: 31296846] (WB, Human) WB Human
Li Y, Xu Y, Jadhav K et al. Hepatic Forkhead Box Protein A3 Regulates ApoA-I (Apolipoprotein A-I) Expression, Cholesterol Efflux, and Atherogenesis Arterioscler. Thromb. Vasc. Biol. Aug 1 2019 [PMID: 31291759] (WB, Mouse) WB Mouse
Chuang WT, Liu YT, Huang CS et al. Benzyl Isothiocyanate and Phenethyl Isothiocyanate Inhibit Adipogenesis and Hepatosteatosis in Mice with Obesity Induced by a High-Fat Diet J. Agric. Food Chem. Jun 26 2019 [PMID: 31240929] (WB, Mouse) WB Mouse
Li CC, Yen CC, Fan CT et al. 14-Deoxy-11,12-didehydroandrographolide suppresses adipogenesis of 3 T3-L1 preadipocytes by inhibiting CCAAT/enhancer-binding protein B activation and AMPK-mediated mitotic clonal expansion. Toxicol. Appl. Pharmacol. Sep 21 2018 [PMID: 30248417] (WB, Mouse) WB Mouse
Ikenoue T, Terakado Y, Zhu C, Liu X. Establishment and analysis of a novel mouse line carrying a conditional knockin allele of a cancer-specific FBXW7 mutation. Sci Rep. 2018 Jan 31 [PMID: 29386660] (WB, Mouse) WB Mouse
Show All 38 Publications.

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Product General Protocols

View specific protocols for SREBP1 Antibody (NB600-582): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SREBP1 Antibody (NB600-582). (Showing 1 - 2 of 2 FAQs).

  1. I would like to order SREBP-1 antibody, catalog number NB600-582. I am wondering if it might cross react with MDCK-C cells from dog?
    • We have not tested NB600-582 with any dog samples, and cannot guarantee that it will cross-react. However, if you are interested in trying this antibody with dog samples you would be eligible for our Innovators Reward Program.
  2. I am looking for SREBF1 antibodies that can cross-react with Zebrafish srebf1. I am wondering whether you have any SREBF1 antibodies that have been tested for zebra fish or have high sequence identity in the immunogen region?
    • Unfortunately we do not have any antibodies that have been tested in zebra fish. I have included the immunogen sequence for the antibodies it might be worth considering. I am not sure exactly what the zebra fish sequence is, I was unable to find it on, so I was unable to check the homology. The following antibodies all have available sample sizes. If you decide to try one you would qualify for our Innovator's Reward Program. NB600-582: 301-407 aa, NB100-2215: 300-400 aa, NB100-60545: 700-800 aa

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-582AF350
Alexa Fluor 405 NB600-582AF405
Alexa Fluor 488 NB600-582AF488
Alexa Fluor 532 NB600-582AF532
Alexa Fluor 594 NB600-582AF594
Alexa Fluor 647 NB600-582AF647
Alexa Fluor 700 NB600-582AF700
Alexa Fluor 750 NB600-582AF750
Biotin NB600-582B
DyLight 350 NB600-582UV
DyLight 405 NB600-582V
DyLight 488 NB600-582G
DyLight 550 NB600-582R
DyLight 594 NB600-582DL594
DyLight 650 NB600-582C
DyLight 680 NB600-582FR
DyLight 755 NB600-582IR
HRP NB600-582H
Janelia Fluor 549 NB600-582JF549
Janelia Fluor 646 NB600-582JF646

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Blogs on SREBP1.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

SREBP2 - regulating cholesterol homeostasis and lipid metabolism
Sterol regulatory element-binding proteins (SREBP) are important transcription factors regulating the synthesis and uptake of lipids including cholesterol. This essential role in lipid metabolism makes investigations into the functions SREBPs im...  Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g....  Read full blog post.

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Gene Symbol SREBF1