SREBP1 Antibody (2A4) - BSA Free

Images

 
Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Hepatic SREBP1c and SREBP2 protein abundance in C57BL/6 mice assigned to a high-fat (HF) or a high-fat/exercise (HF + EX) group for 8 weeks. Cytosolic and nuclear ...read more
Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Analysis of whole cell lysate from HeLa cells showing a single specific band for the expression of SREBP1 precursor protein (~120 kDa).
Simple Western: SREBP1 Antibody (2A4) [NB600-582] - Lane view shows a specific band for SREBP1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Genetic Strategies: Knockout Validated: SREBP1 Antibody (2A4) [NB600-582] - KO and OE of SREBP1 influenced lipid metabolism in Bel-7402 cells. The protein level of lipid-associated genes in SREBP1-KO, SREBP1-OE, ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Ch, Ha, Pm, SyHaSpecies Glossary
Applications WB, Simple Western, IHC-Fr, KO
Clone
2A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

SREBP1 Antibody (2A4) - BSA Free Summary

Immunogen
6 His-tag fusion protein of human SREBP1 corresponding to amino acids 301-407. [UniProt# P36956]
Localization
Cytoplasmic and nuclear
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SREBF1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Frozen
  • Knockout Validated
  • Simple Western 1:12.5
  • Western Blot 1-2ug/ml
Application Notes
This SREBP1 (clone 2A4) antibody is useful for WB where a band can be seen at 125 kDa (precursor) and additional bands may be seen at 60-70 kDa (cleaved).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The use of this antibody in IHC paraffin embedded tissue has been questionable.
Control
HeLa Nuclear Cell Lysate
Publications
Read Publications using
NB600-582 in the following applications:

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 23720350). Hamster reactivity reported in scientific literature (PMID: 24393244). Chicken reactivity reported in multiple pieces scientific literature. Monkey reactivity reported in scientific literature (PMID: 26437365).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for SREBP1 Antibody (2A4) - BSA Free

  • BHLHD1
  • Class D basic helix-loop-helix protein 1
  • SREBP 1c
  • SREBP-1
  • SREBP1bHLHd1SREBP-1c
  • sterol regulatory element binding protein-1
  • sterol regulatory element binding transcription factor 1
  • sterol regulatory element-binding protein 1
  • Sterol regulatory element-binding transcription factor 1

Background

SREBP1 (sterol regulatory element binding protein 1, also called SREBF1 or BHLHD1) is a bHLH-LZ (basic helix-loop-helix-leucine zipper) transcription factor that acts as a key lipogenic transcription factor controlled by glucose/insulin and preferentially regulates lipogenesis via activating genes involved in fatty acid as well as triglyceride synthesis. It is a transcriptional activator required for lipid homeostasis and regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. SREBP1 has a dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (sterol regulatory element 1; 5'-ATCACCCCAC-3'). It exists as multi-pass membrane protein in the membranes of ER, Golgi apparatus and cytoplasmic vesicle /COPII-coated vesicles and moves from ER to Golgi in the absence of sterols. It forms a tight complex with SCAP in ER membranes and interacts with LMNA. AMPK phosphorylate SREBP1 leading to suppression of protein processing and nuclear translocation, and target gene expression repression. Moreover, SIK1 mediated phosphorylation at Ser-402 site represses its activity through inhibition of DNA-binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-582
Species: Hu, Mu, Rt, Ca, Ch, Ha, Pm, SyHa
Applications: WB, Simple Western, IHC-Fr, KO

Publications for SREBP1 Antibody (NB600-582)(46)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Canine, Chicken, Hamster.

We have publications tested in 3 applications: ICC/IF, IHC-F, WB.


Filter By Application
ICC/IF
(1)
IHC-F
(1)
WB
(38)
All Applications
Filter By Species
Human
(9)
Mouse
(20)
Rat
(7)
Canine
(1)
Chicken
(2)
Hamster
(2)
All Species
Showing Publications 1 - 10 of 46. Show All 46 Publications.
Publications using NB600-582 Applications Species
Zeng H, Qin H, Liao M et al. CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing Molecular metabolism Dec 30 2021 [PMID: 34974159] (WB, Mouse) WB Mouse
Jin M, Zhang D, Zheng L et al. Lipopolysaccharide and tyloxapol accelerate the development of atherosclerosis in mice Lipids Dec 7 2021 [PMID: 34875723] (WB, Mouse) WB Mouse
Zhang M, Ceyhan Y, Kaftanovskaya EM et al. INPP4B protects from metabolic syndrome and associated disorders Communications biology Mar 26 2021 [PMID: 33772116] (WB, Mouse) WB Mouse
Cook J. Toward a Mechanistic Understanding of Hepatic Insulin Action and Resistance. Thesis. 2014 (WB, Mouse) WB Mouse
Aliluev A, Tritschler S, Sterr M et al. Diet-induced alteration of intestinal stem cell function underlies obesity and prediabetes in mice Nature metabolism Sep 1 2021 [PMID: 34552271] (WB, Mouse) WB Mouse
Ploner C, Rauchenwald T, Connolly CE et al. Oxidant therapy improves adipogenic differentiation of adipose-derived stem cells in human wound healing Stem cell research & therapy May 10 2021 [PMID: 33971957] (WB, Human) WB Human
Bosc C, Broin N, Fanjul M et al. Autophagy regulates fatty acid availability for oxidative phosphorylation through mitochondria-endoplasmic reticulum contact sites Nat Commun 2020 Aug 13 [PMID: 32792483] (WB, Human) WB Human
Srinivasan MP, Bhopale KK, Caracheo AA et al. Activation of AMP-activated protein kinase attenuates ethanol-induced ER/oxidative stress and lipid phenotype in human pancreatic acinar cells Biochem Pharmacol Oct 20 2020 [PMID: 32717227] (WB, Human, Rat) WB Human, Rat
Shen B, Feng H et al. Geniposide alleviates non-alcohol fatty liver disease via regulating Nrf2/AMPK/mTOR signalling pathways. J Cell Mol Med Jan 5 2020 [PMID: 32293113] (WB, Human) WB Human
Zhou J, Lu Y, Jia Y Et al. Ketogenic Diet Ameliorates Lipid Dysregulation in Type 2 Diabetic Mice by Downregulating Hepatic Pescadillo 1 Research Square Oct 6 2021 (ICC/IF, Rat) ICC/IF Rat
Show All 46 Publications.

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Product General Protocols

View specific protocols for SREBP1 Antibody (NB600-582): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SREBP1 Antibody (NB600-582). (Showing 1 - 2 of 2 FAQs).

  1. I would like to order SREBP-1 antibody, catalog number NB600-582. I am wondering if it might cross react with MDCK-C cells from dog?
    • We have not tested NB600-582 with any dog samples, and cannot guarantee that it will cross-react. However, if you are interested in trying this antibody with dog samples you would be eligible for our Innovators Reward Program.
  2. I am looking for SREBF1 antibodies that can cross-react with Zebrafish srebf1. I am wondering whether you have any SREBF1 antibodies that have been tested for zebra fish or have high sequence identity in the immunogen region?
    • Unfortunately we do not have any antibodies that have been tested in zebra fish. I have included the immunogen sequence for the antibodies it might be worth considering. I am not sure exactly what the zebra fish sequence is, I was unable to find it on uniprot.org, so I was unable to check the homology. The following antibodies all have available sample sizes. If you decide to try one you would qualify for our Innovator's Reward Program. NB600-582: 301-407 aa, NB100-2215: 300-400 aa, NB100-60545: 700-800 aa

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for SREBP1 Antibody (NB600-582)

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Blogs on SREBP1.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

SREBP2 - regulating cholesterol homeostasis and lipid metabolism
Sterol regulatory element-binding proteins (SREBP) are important transcription factors regulating the synthesis and uptake of lipids including cholesterol. This essential role in lipid metabolism makes investigations into the functions SREBPs im...  Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. ...  Read full blog post.

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Bioinformatics

Gene Symbol SREBF1
Entrez
Uniprot