Thyroglobulin Antibody (2H11 + 6E1)

Images

 
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Mouse thyroid tissue section stained with Thyroglobulin Antibody (2H11 + 6E1). IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).
Immunohistochemistry: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Imaging of Mouse stomach tissue. Positive staining can only be observed inside the thyroid cells and the follicle in thyroid tissue. This image ...read more
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. IHC-P image submitted by a verified customer ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Flow, IHC, IHC-P
Clone
2H11 + 6E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Thyroglobulin Antibody (2H11 + 6E1) Summary

Immunogen
Human thyroid follicular cells
Localization
Cytoplasmic and secreted
Marker
Thyroidal Cell Marker
Specificity
Thyroglobulin is a 660kDa dimeric pre-protein with multiple glycosylation sites. It is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. Prior to forming dimers, thyroglobulin monomers undergo conformational maturation in the endoplasmic reticulation. The vast majority of follicular carcinomas of the thyroid will give positive immunoreactivity for anti-thyroglobulin even though sometimes only focally. Poorly differentiated carcinomas of the thyroid are frequently anti-thyroglobulin negative. Adenocarcinomas of other-than-thyroid origin do not react with this antibody. This antibody is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of thyroglobulin in metastatic lesions establishes the thyroid origin of tumor. Anti-thyroglobulin, combined with anti-calcitonin, can identify medullary carcinomas of the thyroid. Furthermore, anti-thyroglobulin, combined with anti-TTF1, can be a reliable marker to differentiate between primary thyroid and lung neoplasms.
Isotype
IgG1 Kappa/IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TG
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5-1.0 ug/million cells
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.1-0.2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
660 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 5
using
NBP2-34294 in the following applications:

Publications
Read Publication using
NBP2-34294 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34530)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Thyroglobulin Antibody (2H11 + 6E1)

  • AITD3
  • AITD3TGN
  • cog
  • TDH3
  • Tg
  • TGN
  • Thyroglobulin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Thyroglobulin Antibody (NBP2-34294)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
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Filter By Species
Mouse
(1)
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Reviews for Thyroglobulin Antibody (NBP2-34294) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using NBP2-34294:
Filter by Applications
Immunohistochemistry-Paraffin
(2)
Immunohistochemistry
(1)
All Applications
Filter by Species
Mouse
(3)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Thyroglobulin NBP2-34294
Enlarge
5
reviewed by:
Abigail Stone
Immunohistochemistry-Paraffin Mouse 10/21/2021
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedThyroid tissue
SpeciesMouse
Lot2H11 + 6E1
Immunohistochemistry Thyroglobulin NBP2-34294
Enlarge
5
reviewed by:
Verified Customer
Immunohistochemistry Mouse 06/20/2017
View

Summary

ApplicationImmunohistochemistry
Sample TestedStomach tissue
SpeciesMouse
Lot7038-3P160707
Immunohistochemistry-Paraffin Thyroglobulin NBP2-34294
Enlarge
5
reviewed by:
Verified Customer
Immunohistochemistry-Paraffin Mouse 06/09/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedThyroid tissue
SpeciesMouse
Lot7038-3P160707

Comments

CommentsImmunofluorescence using paraffin-embedded mouse thyroid tissue section. Antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in antibody diluent reagent) for 12 hours at 4°C.

Product General Protocols

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FAQs for Thyroglobulin Antibody (NBP2-34294) (0)

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Blogs on Thyroglobulin.

TTF1 / NKX2.1 - An essential regulator of lung development with implications in cancer diagnostics
Thyroid transcription factor 1 (TTF-1), also known as NKX2.1, is a conserved master regulatory transcription factor involved in the development of the lung, brain, and thyroid (1). In the lung TTF-1 positively regulates the expression of several lu...  Read full blog post.

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Abigail Stone
10/21/2021
Application: Immunohistochemistry-Paraffin
Species: Mouse

Verified Customer
06/20/2017
Application: Immunohistochemistry
Species: Mouse

Verified Customer
06/09/2017
Application: Immunohistochemistry-Paraffin
Species: Mouse

Bioinformatics

Gene Symbol TG
Uniprot