PEDFR/PNPLA2/ATGL Antibody [Unconjugated]


Western blot shows lysates of human adipose tissue and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat PEDF R/PNPLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # more
PEDF R/PNPLA2 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Sheep Anti-Human/Mouse/Rat PEDF R/PNPLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5365) overnight at 4 °C. more
Simple Western lane view shows lysates of mouse adipose tissue, loaded at 0.2 mg/mL. A specific band was detected for PEDF R/PNPLA2 at approximately 59 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse/Rat more
Loss-of-function analyses of PEDF in Bkid cells. (A) PEDF micro RNA (miR) expressing vector was transfected into Bkid cells. Immunoblot by anti-PEDF antibody showed the reduction of PEDF proteins by miRs. (B) The renal more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC

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PEDFR/PNPLA2/ATGL Antibody [Unconjugated] Summary

E. coli-derived recombinant mouse PEDF R/PNPLA2
Accession # Q8BJ56
Detects human, mouse, and rat PEDF R/PNPLA2 in direct ELISAs and Western blots.
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Read Publications using
AF5365 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PEDFR/PNPLA2/ATGL Antibody [Unconjugated]

  • Adipose triglyceride lipase
  • ATGL
  • ATGL1110001C14Rik
  • Calcium-independent phospholipase A2
  • Desnutrin
  • EC
  • FP17548
  • IPLA2-zeta
  • patatin-like phospholipase domain containing 2
  • patatin-like phospholipase domain-containing protein 2
  • patatin-like phospholipase domain-containing protein 2-like
  • PEDF R
  • PEDF-R
  • Pigment epithelium-derived factor
  • PNPLA2
  • Transport-secretion protein 2
  • transport-secretion protein 2.2
  • triglyceride hydrolase
  • TTS2
  • TTS2.2
  • TTS-2.2
  • TTS2DKFZp667M109


PEDF R (Pigment epithelium derived factor receptor; also known as ATGL, PNPLA2, and Desnutrin) is a 55 kDa member of the PNPLA family of proteins. It is expressed in adipocytes, hepatocytes, skeletal muscle, and pigment epithelium. PEDF R specifically hydrolyses triglycerides, releasing long-chain fatty acids. Mouse PEDF R is described as being a 486 amino acid (aa) type II transmembrane (TM) protein. Its TM segment is stated to span aa 9-29. Alternatively, it may have a hydrophobic lipid-association domain between aa 267-295. It contains a palatin domain (aa 10-179) with a conserved GxSxG enzymatic motif. There are multiple splice forms. Two show alternate start sites at Met57 and Met232, while two others show a deletion of aa 163-196 and aa 253-308, respectively. Over aa 162-253, mouse PEDF R shares 98% and 93% aa sequence identity with rat and human PEDF R, respectively.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PEDFR/PNPLA2/ATGL Antibody (AF5365)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC, IHC, Western Blot.

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Showing Publications 1 - 6 of 6.
Publications using AF5365 Applications Species
Sun, L;Zhang, X;Wu, S;Liu, Y;Guerrero-Juarez, CF;Liu, W;Huang, J;Yao, Q;Yin, M;Li, J;Ramos, R;Liao, Y;Wu, R;Xia, T;Zhang, X;Yang, Y;Li, F;Heng, S;Zhang, W;Yang, M;Tzeng, CM;Ji, C;Plikus, MV;Gallo, RL;Zhang, LJ; Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration Cell reports 2023-06-16 [PMID: 37330908] (ICC, Mouse) ICC Mouse
JY Kim, S Park, SH Park, D Lee, GH Kim, JE Noh, KJ Lee, GJ Kim Overexpression of pigment epithelium-derived factor in placenta-derived mesenchymal stem cells promotes mitochondrial biogenesis in retinal cells Lab. Invest., 2020-07-28;0(0):. 2020-07-28 [PMID: 32724163] (Western Blot, Rat) Western Blot Rat
S Omri, H Tahiri, WC Pierre, M Desjarlais, I Lahaie, SE Loiselle, F Rezende, G Lodygensky, TE Hebert, H Ong, S Chemtob Propranolol Attenuates Proangiogenic Activity of Mononuclear Phagocytes: Implication in Choroidal Neovascularization Invest. Ophthalmol. Vis. Sci., 2019-11-01;60(14):4632-4642. 2019-11-01 [PMID: 31682714] (IHC, Mouse) IHC Mouse
W Eichler, H Savkovi?-C, S Bürger, M Beck, M Schmidt, P Wiedemann, A Reichenbac, JD Unterlauft M�ller Cell-Derived PEDF Mediates Neuroprotection via STAT3 Activation Cell. Physiol. Biochem., 2017-11-30;44(4):1411-1424. 2017-11-30 [PMID: 29186716] (Western Blot, Mouse, Rat) Western Blot Mouse, Rat
D Martinez-M, C Jarvis, T Nelius, W de Riese, OV Volpert, S Filleur PEDF increases the tumoricidal activity of macrophages towards prostate cancer cells in vitro PLoS ONE, 2017-04-12;12(4):e0174968. 2017-04-12 [PMID: 28403150] (ICC, Mouse) ICC Mouse
Deshpande M, Notari L, Subramanian P, Notario V, Becerra SP Inhibition of tumor cell surface ATP synthesis by pigment epithelium-derived factor: implications for antitumor activity. Int. J. Oncol., 2012-04-10;41(1):219-27. 2012-04-10 [PMID: 22504705] (Western Blot, Human) Western Blot Human

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Gene Symbol PNPLA2