PEDFR/PNPLA2/ATGL Antibody [Unconjugated]

Images

 
Western blot shows lysates of human adipose tissue and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat PEDF R/PNPLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
PEDF R/PNPLA2 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Sheep Anti-Human/Mouse/Rat PEDF R/PNPLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5365) overnight at 4 °C. ...read more
Simple Western lane view shows lysates of mouse adipose tissue, loaded at 0.2 mg/mL. A specific band was detected for PEDF R/PNPLA2 at approximately 59 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse/Rat ...read more
Loss-of-function analyses of PEDF in Bkid cells. (A) PEDF micro RNA (miR) expressing vector was transfected into Bkid cells. Immunoblot by anti-PEDF antibody showed the reduction of PEDF proteins by miRs. (B) The renal ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

PEDFR/PNPLA2/ATGL Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse PEDF R/PNPLA2
Val162-Gly253
Accession # Q8BJ56
Specificity
Detects human, mouse, and rat PEDF R/PNPLA2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PNPLA2
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF5365 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PEDFR/PNPLA2/ATGL Antibody [Unconjugated]

  • Adipose triglyceride lipase
  • ATGL
  • ATGL1110001C14Rik
  • Calcium-independent phospholipase A2
  • Desnutrin
  • EC 3.1.1.3
  • FP17548
  • IPLA2-zeta
  • patatin-like phospholipase domain containing 2
  • patatin-like phospholipase domain-containing protein 2
  • patatin-like phospholipase domain-containing protein 2-like
  • PEDF R
  • PEDFR
  • PEDF-R
  • Pigment epithelium-derived factor
  • PNPLA2
  • Transport-secretion protein 2
  • transport-secretion protein 2.2
  • triglyceride hydrolase
  • TTS2
  • TTS2.2
  • TTS-2.2
  • TTS2DKFZp667M109

Background

PEDF R (Pigment epithelium derived factor receptor; also known as ATGL, PNPLA2, and Desnutrin) is a 55 kDa member of the PNPLA family of proteins. It is expressed in adipocytes, hepatocytes, skeletal muscle, and pigment epithelium. PEDF R specifically hydrolyses triglycerides, releasing long-chain fatty acids. Mouse PEDF R is described as being a 486 amino acid (aa) type II transmembrane (TM) protein. Its TM segment is stated to span aa 9-29. Alternatively, it may have a hydrophobic lipid-association domain between aa 267-295. It contains a palatin domain (aa 10-179) with a conserved GxSxG enzymatic motif. There are multiple splice forms. Two show alternate start sites at Met57 and Met232, while two others show a deletion of aa 163-196 and aa 253-308, respectively. Over aa 162-253, mouse PEDF R shares 98% and 93% aa sequence identity with rat and human PEDF R, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PEDFR/PNPLA2/ATGL Antibody (AF5365)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC, IHC, Western Blot.


Filter By Application
ICC
(2)
IHC
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
Rat
(2)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF5365 Applications Species
Sun, L;Zhang, X;Wu, S;Liu, Y;Guerrero-Juarez, CF;Liu, W;Huang, J;Yao, Q;Yin, M;Li, J;Ramos, R;Liao, Y;Wu, R;Xia, T;Zhang, X;Yang, Y;Li, F;Heng, S;Zhang, W;Yang, M;Tzeng, CM;Ji, C;Plikus, MV;Gallo, RL;Zhang, LJ; Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration Cell reports 2023-06-16 [PMID: 37330908] (ICC, Mouse) ICC Mouse
JY Kim, S Park, SH Park, D Lee, GH Kim, JE Noh, KJ Lee, GJ Kim Overexpression of pigment epithelium-derived factor in placenta-derived mesenchymal stem cells promotes mitochondrial biogenesis in retinal cells Lab. Invest., 2020-07-28;0(0):. 2020-07-28 [PMID: 32724163] (Western Blot, Rat) Western Blot Rat
S Omri, H Tahiri, WC Pierre, M Desjarlais, I Lahaie, SE Loiselle, F Rezende, G Lodygensky, TE Hebert, H Ong, S Chemtob Propranolol Attenuates Proangiogenic Activity of Mononuclear Phagocytes: Implication in Choroidal Neovascularization Invest. Ophthalmol. Vis. Sci., 2019-11-01;60(14):4632-4642. 2019-11-01 [PMID: 31682714] (IHC, Mouse) IHC Mouse
W Eichler, H Savkovi?-C, S Bürger, M Beck, M Schmidt, P Wiedemann, A Reichenbac, JD Unterlauft M�ller Cell-Derived PEDF Mediates Neuroprotection via STAT3 Activation Cell. Physiol. Biochem., 2017-11-30;44(4):1411-1424. 2017-11-30 [PMID: 29186716] (Western Blot, Mouse, Rat) Western Blot Mouse, Rat
D Martinez-M, C Jarvis, T Nelius, W de Riese, OV Volpert, S Filleur PEDF increases the tumoricidal activity of macrophages towards prostate cancer cells in vitro PLoS ONE, 2017-04-12;12(4):e0174968. 2017-04-12 [PMID: 28403150] (ICC, Mouse) ICC Mouse
Deshpande M, Notari L, Subramanian P, Notario V, Becerra SP Inhibition of tumor cell surface ATP synthesis by pigment epithelium-derived factor: implications for antitumor activity. Int. J. Oncol., 2012-04-10;41(1):219-27. 2012-04-10 [PMID: 22504705] (Western Blot, Human) Western Blot Human

Reviews for PEDFR/PNPLA2/ATGL Antibody (AF5365) (0)

There are no reviews for PEDFR/PNPLA2/ATGL Antibody (AF5365). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PEDFR/PNPLA2/ATGL Antibody (AF5365) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PEDFR/PNPLA2/ATGL Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol PNPLA2
Uniprot