Rhodopsin Antibody (B630)


Western Blot: Rhodopsin Antibody (B630) [NBP2-25160] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of ...read more
Immunocytochemistry/ Immunofluorescence: Rhodopsin Antibody (B630) [NBP2-25160] - High magnification confocal image of pig retinal section stained with NBP2-25160 (Green). Rhodopsin is most abundant in the rod outer ...read more

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, Ca, EqSpecies Glossary
Applications WB, ICC/IF, IHC
1 mg/ml

Rhodopsin Antibody (B630) Summary

Purified rhodopsin from bovine retina.
Epitope resides in the N-terminal 32 amino acids.
Membrane; Multi-pass membrane protein. Note: Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:5000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
Application Notes
This Rhodopsin (B630) antibody is useful for Immunocytochemisty/Immunofluorescence, Immunohistochemistry, and Western Blot, where a band can be seen at ~35 kDa.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NBP2-25160 in the following applications:

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 30889179).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 50% glycerol (1:1)
5mM Sodium Azide
1 mg/ml
Protein G purified

Alternate Names for Rhodopsin Antibody (B630)

  • MGC138309
  • OPN2MGC138311
  • opsin 2, rod pigment
  • opsin-2
  • retinitis pigmentosa 4, autosomal dominant
  • rhodopsin
  • RP4


Rhodopsin is the protein in the mammalian retina responsible for the light sensitivity of rod cells, which are responsible for vision in low light levels. Somewhat surprisingly, the Rhodopsin protein turned out to be a typical member of the seven transmembrane G protein-coupled receptor (GPCR) superfamily. Whereas other GPCRs initiate signaling on binding a specific ligand, Rhodopsin exists with a ligand already bound, specifically the vitamin A related substance retinal. Retinal can exist in two isomeric forms, 11-cis and 11-trans retinal. In the dark Rhodopsin is associated with 11-cis retinal, but photons cause the 11-cis form to flip to the 11-trans form, and this causes an alteration in the structure of the Rhodopsin making it catalytically active. Activated Rhodopsin in turn activates the GTP binding protein transducin by favoring the loss of GDP and the addition of GTP. Transducin is a typical member of the family of heterotrimeric G proteins, and consists of an alpha and a beta gamma subunit. The alpha subunit is responsible for the GTP binding and the GTP bound form activates a phosphodiesterase (PDE) enzyme which hydrolysescyclic GMP. This in turn results in the closure of cyclic nucleotide gated ion channels in the rod cell membrane, which prevent the influx of sodium and calcium ions. This results in an increase in the membrane potential of the rod cell and so reduces the rate of synaptic signaling. So light stimulation results in a reduced rate of photoreceptor synaptic release. This information is transmitted through neurons of the retina to the visual centers of the brain. Rhodopsin activity is shut off by phosphorylation under the influence of Rhodopsin kinase, the activity of which results in binding of visual arrestin, which prevents Rhodopsin from interacting with and activating more transducin molecules. This basic signaling paradigm proved to be a prototype for understanding how other GPCRs function, as proteins similar to transducin, arrestin and rhodopsin kinase are found in these pathways.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Rb
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Ca, Pm, Rb
Applications: WB, ICC/IF
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Mu
Applications: Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, IHC, IHC-P, IP
Species: Hu, Bv
Applications: WB, ICC/IF, IHC, IHC-Fr, IP
Species: Hu, Mu
Applications: WB, ICC/IF, IHC-P
Species: Hu, Mu
Applications: WB, Flow, CyTOF-ready, ICC
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Pm, Pm
Applications: IHC, IHC-P
Species: Hu, Pm
Applications: IHC, IHC-P, ICC
Species: Hu, Rt, Pm
Applications: IHC, IHC-P
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq
Applications: WB, ICC/IF, IHC

Publications for Rhodopsin Antibody (NBP2-25160)(2)

We have publications tested in 1 confirmed species: Canine.

We have publications tested in 2 applications: ICC/IF, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 2 of 2.
Publications using NBP2-25160 Applications Species
MAkelAinen S, Hellsand M, van der Heiden A et al. Deletion in the Bardet-Biedl Syndrome Gene TTC8 Results in a Syndromic Retinal Degeneration in Dogs Preprint Aug 26 2020
Makelainen, S;Godia, M;Hellsand, M;Viluma, A;Hahn, D;Makdoumi, K;Zeiss, CJ;Mellersh, C;Ricketts, SL;Narfstrom, K;Hallbook, F;Ekesten, B;Andersson, G;Bergstrom, TF; An ABCA4 loss-of-function mutation causes a canine form of Stargardt disease PLoS Genet. Mar 1 2019 [PMID: 30889179] (WB, ICC/IF, Canine) WB, ICC/IF Canine

Reviews for Rhodopsin Antibody (NBP2-25160) (0)

There are no reviews for Rhodopsin Antibody (NBP2-25160). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rhodopsin Antibody (NBP2-25160). (Showing 1 - 1 of 1 FAQs).

  1. I’m looking for anti-bovine rhodopsin antibody recognizing extracellular part of protein. Because I’m using chimera protein containing only extracellular part of rhodopsin it is crucial that epitope is localized outside of cell. I came across your product
    • Unfortunately, for product NBP2-25160, we have not yet mapped the epitope, so we would not know for sure if it will recognize the extracellular or cytoplasmic domain of the rhodopsin protein.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-25160AF405
Alexa Fluor 488 NBP2-25160AF488
Alexa Fluor 647 NBP2-25160AF647
Alexa Fluor 700 NBP2-25160AF700
Biotin NBP2-25160B
DyLight 350 NBP2-25160UV
DyLight 405 NBP2-25160V
DyLight 488 NBP2-25160G
DyLight 550 NBP2-25160R
DyLight 650 NBP2-25160C
DyLight 680 NBP2-25160FR
DyLight 755 NBP2-25160IR
FITC NBP2-25160F
HRP NBP2-25160H
Janelia Fluor 549 NBP2-25160JF549
Janelia Fluor 646 NBP2-25160JF646

Additional Rhodopsin Products

Bioinformatics Tool for Rhodopsin Antibody (NBP2-25160)

Discover related pathways, diseases and genes to Rhodopsin Antibody (NBP2-25160). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Rhodopsin Antibody (NBP2-25160)

Discover more about diseases related to Rhodopsin Antibody (NBP2-25160).

Pathways for Rhodopsin Antibody (NBP2-25160)

View related products by pathway.

PTMs for Rhodopsin Antibody (NBP2-25160)

Learn more about PTMs related to Rhodopsin Antibody (NBP2-25160).

Research Areas for Rhodopsin Antibody (NBP2-25160)

Find related products by research area.

Blogs on Rhodopsin.

Vision Infographic: Do you see how I see?
Vision involves several parts of the eye processing light which send signals to the brain via the optic nerve to process information. Learn more about the vision process and related ocular proteins in the infographic below. Novus Biological...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Rhodopsin Antibody (B630) and receive a gift card or discount.


Gene Symbol RHO