Rhodopsin Antibody (B630)

Images

 
Western Blot: Rhodopsin Antibody (B630) [NBP2-25160] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of ...read more
Immunocytochemistry/ Immunofluorescence: Rhodopsin Antibody (B630) [NBP2-25160] - High magnification confocal image of pig retinal section stained with NBP2-25160 (Green). Rhodopsin is most abundant in the rod outer ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, EqSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
B630
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Rhodopsin Antibody (B630) Summary

Immunogen
Purified rhodopsin from bovine retina.
Epitope
Epitope resides in the N-terminal 32 amino acids.
Localization
Membrane; Multi-pass membrane protein. Note: Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RHO
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
Application Notes
This Rhodopsin (B630) antibody is useful for Immunocytochemisty/Immunofluorescence, Immunohistochemistry, and Western Blot, where a band can be seen at ~35 kDa.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-25160 in the following applications:

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 30889179).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50% PBS, 50% Glycerol
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Rhodopsin Antibody (B630)

  • CSNBAD1
  • MGC138309
  • OPN2MGC138311
  • opsin 2, rod pigment
  • opsin-2
  • retinitis pigmentosa 4, autosomal dominant
  • rhodopsin
  • RP4

Background

Rhodopsin is the protein in the mammalian retina responsible for the light sensitivity of rod cells, which are responsible for vision in low light levels. Somewhat surprisingly, the Rhodopsin protein turned out to be a typical member of the seven transmembrane G protein-coupled receptor (GPCR) superfamily. Whereas other GPCRs initiate signaling on binding a specific ligand, Rhodopsin exists with a ligand already bound, specifically the vitamin A related substance retinal. Retinal can exist in two isomeric forms, 11-cis and 11-trans retinal. In the dark Rhodopsin is associated with 11-cis retinal, but photons cause the 11-cis form to flip to the 11-trans form, and this causes an alteration in the structure of the Rhodopsin making it catalytically active. Activated Rhodopsin in turn activates the GTP binding protein transducin by favoring the loss of GDP and the addition of GTP. Transducin is a typical member of the family of heterotrimeric G proteins, and consists of an alpha and a beta gamma subunit. The alpha subunit is responsible for the GTP binding and the GTP bound form activates a phosphodiesterase (PDE) enzyme which hydrolysescyclic GMP. This in turn results in the closure of cyclic nucleotide gated ion channels in the rod cell membrane, which prevent the influx of sodium and calcium ions. This results in an increase in the membrane potential of the rod cell and so reduces the rate of synaptic signaling. So light stimulation results in a reduced rate of photoreceptor synaptic release. This information is transmitted through neurons of the retina to the visual centers of the brain. Rhodopsin activity is shut off by phosphorylation under the influence of Rhodopsin kinase, the activity of which results in binding of visual arrestin, which prevents Rhodopsin from interacting with and activating more transducin molecules. This basic signaling paradigm proved to be a prototype for understanding how other GPCRs function, as proteins similar to transducin, arrestin and rhodopsin kinase are found in these pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rhodopsin Antibody (NBP2-25160)(1)

We have publications tested in 1 confirmed species: Canine.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rhodopsin Antibody (NBP2-25160). (Showing 1 - 1 of 1 FAQ).

  1. I’m looking for anti-bovine rhodopsin antibody recognizing extracellular part of protein. Because I’m using chimera protein containing only extracellular part of rhodopsin it is crucial that epitope is localized outside of cell. I came across your product
    • Unfortunately, for product NBP2-25160, we have not yet mapped the epitope, so we would not know for sure if it will recognize the extracellular or cytoplasmic domain of the rhodopsin protein.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-25160AF405
Alexa Fluor 488 NBP2-25160AF488
Alexa Fluor 647 NBP2-25160AF647
Alexa Fluor 700 NBP2-25160AF700
Biotin NBP2-25160B
DyLight 350 NBP2-25160UV
DyLight 405 NBP2-25160V
DyLight 488 NBP2-25160G
DyLight 550 NBP2-25160R
DyLight 650 NBP2-25160C
DyLight 680 NBP2-25160FR
DyLight 755 NBP2-25160IR
FITC NBP2-25160F
HRP NBP2-25160H
Janelia Fluor 549 NBP2-25160JF549
Janelia Fluor 646 NBP2-25160JF646

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Vision involves several parts of the eye processing light which send signals to the brain via the optic nerve to process information. Learn more about the vision process and related ocular proteins in the infographic below. Novus Biological...  Read full blog post.

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Bioinformatics

Gene Symbol RHO
Entrez
OMIM
Uniprot
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