Rhodopsin Antibody (B630)

Images

 
Immunohistochemistry: Rhodopsin Antibody (B630) [NBP2-25160] - Rhodopsin expression and the prevalence of rod photoreceptors in the canine retina. (A) Fluorescence micrographs showing rhodopsin expression (red) in the ...read more
Western Blot: Rhodopsin Antibody (B630) [NBP2-25160] - Western blot analyses of ABCA4 (above), GAPDH (middle), and RHO (below) protein levels in retinal tissue of dogs with the three different genotypes. Image collected ...read more
Immunocytochemistry/ Immunofluorescence: Rhodopsin Antibody (B630) [NBP2-25160] - High magnification confocal image of pig retinal section stained with NBP2-25160 (Green). Rhodopsin is most abundant in the rod outer ...read more
Western Blot: Rhodopsin Antibody (B630) [NBP2-25160] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, EqSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
B630
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Rhodopsin Antibody (B630) Summary

Immunogen
Purified rhodopsin from bovine retina.
Epitope
Epitope resides in the N-terminal 32 amino acids.
Localization
Membrane; Multi-pass membrane protein. Note: Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RHO
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Western Blot 1:5000
Application Notes
This Rhodopsin (B630) antibody is useful for Immunocytochemisty/Immunofluorescence, Immunohistochemistry, and Western Blot, where a band can be seen at ~35 kDa.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-25160 in the following applications:

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 30889179).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50% PBS, 50% glycerol
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Rhodopsin Antibody (B630)

  • CSNBAD1
  • MGC138309
  • OPN2
  • OPN2MGC138311
  • opsin 2, rod pigment
  • Opsin-2
  • retinitis pigmentosa 4, autosomal dominant
  • RHO
  • Rhodopsin
  • RP4

Background

Rhodopsin is the protein in the mammalian retina responsible for the light sensitivity of rod cells, which are responsible for vision in low light levels. Somewhat surprisingly, the Rhodopsin protein turned out to be a typical member of the seven transmembrane G protein-coupled receptor (GPCR) superfamily. Whereas other GPCRs initiate signaling on binding a specific ligand, Rhodopsin exists with a ligand already bound, specifically the vitamin A related substance retinal. Retinal can exist in two isomeric forms, 11-cis and 11-trans retinal. In the dark Rhodopsin is associated with 11-cis retinal, but photons cause the 11-cis form to flip to the 11-trans form, and this causes an alteration in the structure of the Rhodopsin making it catalytically active. Activated Rhodopsin in turn activates the GTP binding protein transducin by favoring the loss of GDP and the addition of GTP. Transducin is a typical member of the family of heterotrimeric G proteins, and consists of an alpha and a beta gamma subunit. The alpha subunit is responsible for the GTP binding and the GTP bound form activates a phosphodiesterase (PDE) enzyme which hydrolysescyclic GMP. This in turn results in the closure of cyclic nucleotide gated ion channels in the rod cell membrane, which prevent the influx of sodium and calcium ions. This results in an increase in the membrane potential of the rod cell and so reduces the rate of synaptic signaling. So light stimulation results in a reduced rate of photoreceptor synaptic release. This information is transmitted through neurons of the retina to the visual centers of the brain. Rhodopsin activity is shut off by phosphorylation under the influence of Rhodopsin kinase, the activity of which results in binding of visual arrestin, which prevents Rhodopsin from interacting with and activating more transducin molecules. This basic signaling paradigm proved to be a prototype for understanding how other GPCRs function, as proteins similar to transducin, arrestin and rhodopsin kinase are found in these pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rhodopsin Antibody (NBP2-25160)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 6 applications: Func, ICC/IF, IHC, IHC-Fr, IHC-WhMt, WB.


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Func
(1)
ICC/IF
(1)
IHC
(3)
IHC-Fr
(1)
IHC-WhMt
(1)
WB
(2)
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Human
(1)
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(2)
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Showing Publications 1 - 8 of 8.
Publications using NBP2-25160 Applications Species
Chen C, Rong Y, Zhuang Y et al. RNA-Seq Analysis Reveals an Essential Role of the cGMP-PKG-MAPK Pathways in Retinal Degeneration Caused by Cep250 Deficiency International Journal of Molecular Sciences 2023-05-16 [PMID: 37240188] (IHC, Func) IHC, Func
Li M, Xu J, Wang Y et al. Astragaloside A Protects Against Photoreceptor Degeneration in Part Through Suppressing Oxidative Stress and DNA Damage-Induced Necroptosis and Inflammation in the Retina Journal of Inflammation Research 2022-05-20 [PMID: 35645574] (IHC, IHC-Fr) IHC, IHC-Fr
Chen C, Liu Q, Rong Y et al. ZC3H11A mutations cause high myopia by triggering PI3K-AKT and NF-?B mediated inflammatory reactions in humans and mice bioRxiv 2023-08-30 (IHC, Mouse) IHC Mouse
Vargas JA, Finnemann SC Differences in Diurnal Rhythm of Rod Outer Segment Renewal between 129T2/SvEmsJ and C57BL/6J Mice International journal of molecular sciences 2022-08-22 [PMID: 36012733] (IHC-WhMt, Mouse) IHC-WhMt Mouse
Mao Y, Finnemann SC Acute RhoA/Rho Kinase Inhibition Is Sufficient to Restore Phagocytic Capacity to Retinal Pigment Epithelium Lacking the Engulfment Receptor MerTK Cells 2021-07-29 [PMID: 34440696]
Bullock J, Polato F, Abu-Asab M et al. Degradation of Photoreceptor Outer Segments by the Retinal Pigment Epithelium Requires Pigment Epithelium-Derived Factor Receptor (PEDF-R) Investigative ophthalmology & visual science 2021-02-01 [PMID: 33605986] (WB, Human) WB Human
MAkelAinen S, Hellsand M, van der Heiden A et al. Deletion in the Bardet-Biedl Syndrome Gene TTC8 Results in a Syndromic Retinal Degeneration in Dogs Genes (Basel) 2020-09-23 [PMID: 32962042]
Makelainen, S;Godia, M;Hellsand, M;Viluma, A;Hahn, D;Makdoumi, K;Zeiss, CJ;Mellersh, C;Ricketts, SL;Narfstrom, K;Hallbook, F;Ekesten, B;Andersson, G;Bergstrom, TF; An ABCA4 loss-of-function mutation causes a canine form of Stargardt disease PLoS Genet. 2019-03-01 [PMID: 30889179] (ICC/IF, WB, Canine) ICC/IF, WB Canine

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rhodopsin Antibody (NBP2-25160). (Showing 1 - 1 of 1 FAQs).

  1. I’m looking for anti-bovine rhodopsin antibody recognizing extracellular part of protein. Because I’m using chimera protein containing only extracellular part of rhodopsin it is crucial that epitope is localized outside of cell. I came across your product
    • Unfortunately, for product NBP2-25160, we have not yet mapped the epitope, so we would not know for sure if it will recognize the extracellular or cytoplasmic domain of the rhodopsin protein.

Secondary Antibodies

 

Isotype Controls

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Blogs on Rhodopsin.

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Bioinformatics

Gene Symbol RHO
Entrez
OMIM
Uniprot