ORP150/HSP12A Antibody - BSA Free

Western Blot: ORP150/HSP12A Antibody [NBP2-14113] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: HELA

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP2-14113] - Staining of human pancreas shows distinct cytoplasmic positivity in exocrine glandular cells.

Simple Western: ORP150/HSP12A Antibody [NBP2-14113] - Simple Western lane view shows a specific band for ORP150/HSP12A in 0.2 mg/ml of HEL lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.

Simple Western: ORP150/HSP12A Antibody [NBP2-14113] - Electropherogram image of the corresponding Simple Western lane view. ORP150/HSP12A antibody was used at 1:25 dilution on HEL lysate(s) respectively.

• Reactivity: Hu, Ch, Mu, Rt
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ORP150/HSP12A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ORP150/HSP12A Antibody - BSA Free (NBP2-14113) is a polyclonal antibody validated for use in IHC, WB and Simple Western. Anti-ORP150/HSP12A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to the amino acids: VLQLINDNTATALSYGVFRRKDINTTAQNIMFYDMGSGSTVCTIVTYQMVKTKEAGMQPQLQIRGVGFDRTLGGLEMELRLRERLAGLFNEQRKGQRAKDVRENPRAMAKLLREANRLKTVLSANADHMAQIEGLMDDVDFKAKVTRV
• Predicted Species: Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HYOU1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Simple Western 1:25
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HEL lysates, separated by Size, antibody dilution of 1:25
• Control Peptide:

  ORP150/HSP12A Protein (NBP2-14113PEP)

Reactivity Notes

Chicken reactivity reported in literature (PMID: 30298003)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ORP150/HSP12A Antibody - BSA Free

• 150 kDa oxygen-regulated protein
• 170 kDa glucose-regulated protein
• DKFZp686N08236
• FLJ97572
• glucose-regulated protein 170
• Grp170
• GRP-170
• HSP12A
• HYOU1
• hypoxia up-regulated 1
• hypoxia up-regulated protein 1
• ORP150
• ORP-150
• ORP150FLJ94899
• oxygen regulated protein (150kD)

Background

HYOU1 is encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment is found at the 5' end of the first exon, and is involved in stress-dependent induction. A transcript that is produced from a downstream start site is preferentially induced by hypoxia, resulting in the accumulation of this protein in the endoplasmic reticulum (ER). The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ORP150/HSP12A Antibody (NBP2-14113)(1)

Publication using NBP2-14113

• Fauzia E, Barbhuyan Tk, Shrivastava Ak et al. Chick Embryo: A Preclinical Model for Understanding Ischemia-Reperfusion Mechanism. Front Pharmacol. 2018-09-21 [PMID: 30298003] (WB, Chicken)

Bioinformatics

• Gene Symbol: HYOU1