ORP150 Antibodies
We offer ORP150/HSP12A Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each ORP150/HSP12A Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ORP150/HSP12A Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the ORP150/HSP12A Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ORP150/HSP12A polyclonal antibodies and browse our ORP150/HSP12A monoclonal antibody catalog.
Alternate Names for ORP150 Antibodies
anti-ORP150/HSP12A antibody, anti-HYOU1 antibody, anti-150 kDa oxygen-regulated protein antibody, anti-170 kDa glucose-regulated protein antibody, anti-DKFZp686N08236 antibody, anti-FLJ97572 antibody, anti-glucose-regulated protein 170 antibody, anti-GRP-170 antibody, anti-Grp170 antibody, anti-HSP12A antibody, anti-hypoxia up-regulated 1 antibody, anti-hypoxia up-regulated protein 1 antibody, anti-ORP-150 antibody, anti-ORP150FLJ94899 antibody, anti-oxygen regulated protein (150kD) antibody
15 Results for "orp150-hsp12a" in Primary Antibodies
![Western Blot: ORP150/HSP12A Antibody [NBP1-32140] - Mouse tissue extracts (50 ug) was separated by 5% SDS-PAGE, and blotted with ORP150 antibodydiluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Western-Blot-NBP1-32140-img0011.jpg "Western Blot: ORP150/HSP12A Antibody [NBP1-32140] - Mouse tissue extracts (50 ug) was separated by 5% SDS-PAGE, and blotted with ORP150 antibodydiluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ORP150 protein stained by ORP150 antibody [C2C3], C-term diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32140-img0015.jpg)
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP1-32140] - Meta-analysis of significant metastatic upregulated proteins in acid exosomes through Prognoscan. Confocal immunofluorescence analysis with the indicated antibodies on primary (apical and mid region), and metastatic melanoma biopsies from the same patient. Ki67 labeling was used as internal control. Red, CFL, GSN, HYOU1; blue, DAPI; green, Ki67. Bar 50 um Image collected and cropped by CiteAb from the following publication (//jeccr.biomedcentral.com/articles/10.1186/s13046-018-0915-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Immunohistochemistry-Paraffin-NBP1-32140-img0016.jpg)
![Western Blot: ORP150/HSP12A Antibody [NBP1-32140] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and blotted with ORP150 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Western-Blot-NBP1-32140-img0008.jpg)
![Western Blot: ORP150/HSP12A Antibody [NBP1-32140] - Rat tissue extracts (50 ug) was separated by 5% SDS-PAGE, and blotted with ORP150 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Western-Blot-NBP1-32140-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] - Analysis of methanol-fixed Hep3B, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32140-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] - U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ORP150 protein stained by ORP150 antibody [C2C3], C-term diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32140-img0013.jpg)
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP1-32140] - Paraffin-embedded CL1-0 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Immunohistochemistry-Paraffin-NBP1-32140-img0006.jpg)
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP1-32140] - Paraffin-embedded mouse fore brain. ORP150 antibody [C2C3], C-term diluted at 1:500.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Immunohistochemistry-Paraffin-NBP1-32140-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: ORP150/HSP12A Antibody [NBP1-32140] - Meta-analysis of significant metastatic upregulated proteins in acid exosomes through Prognoscan. a Kaplan-Meyer curves depicting the Overall Survival of metastatic melanoma patients with high (red lines) or low (green lines) expression of the genes listed in Table 1 that resulted statistically significant. Mentel-Cox p-values for each plot are showed. b Western blotting analysis of ctr & pH 6.0 exosomes (20 μg). Cell lysate was used as positive control. Expression of CFL, GSN & HSP90 alpha beta is shown. Ponceau red is shown for equal exosome protein amount. c confocal immunofluorescence analysis with the indicated antibodies on primary (apical & mid region), & metastatic melanoma biopsies from the same patient. Ki67 labeling was used as internal control. Red, CFL, GSN, HYOU1; blue, DAPI; green, Ki67. Bar 50 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30290833), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32140_rabbit-polyclonal-orp150-hsp12a-antibody-31020241615469.jpg)
![Western Blot: ORP150/HSP12A Antibody (6F7) [H00010525-M01] - HYOU1 monoclonal antibody (M01), clone 6F7 Analysis of HYOU1 expression in MCF-7.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-6F7-Western-Blot-H00010525-M01-img0010.jpg "Western Blot: ORP150/HSP12A Antibody (6F7) [H00010525-M01] - HYOU1 monoclonal antibody (M01), clone 6F7 Analysis of HYOU1 expression in MCF-7.")
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (6F7) [H00010525-M01] - Analysis of monoclonal antibody to HYOU1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-6F7-Immunohistochemistry-Paraffin-H00010525-M01-img0008.jpg)
![ELISA: ORP150/HSP12A Antibody (6F7) [H00010525-M01] - Detection limit for recombinant GST tagged HYOU1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-6F7-ELISA-H00010525-M01-img0011.jpg)
![Western Blot: ORP150/HSP12A Antibody [NBP2-14113] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: HELA](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Western-Blot-NBP2-14113-img0002.jpg "Western Blot: ORP150/HSP12A Antibody [NBP2-14113] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: HELA")
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP2-14113] - Staining of human pancreas shows distinct cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Immunohistochemistry-Paraffin-NBP2-14113-img0001.jpg)
![Simple Western: ORP150/HSP12A Antibody [NBP2-14113] - Simple Western lane view shows a specific band for ORP150/HSP12A in 0.2 mg/ml of HEL lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Simple-Western-NBP2-14113-img0003.jpg)
![Simple Western: ORP150/HSP12A Antibody [NBP2-14113] - Electropherogram image of the corresponding Simple Western lane view. ORP150/HSP12A antibody was used at 1:25 dilution on HEL lysate(s) respectively.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Simple-Western-NBP2-14113-img0004.jpg)
![Western Blot: ORP150/HSP12A Antibody (201) [NBP2-89890] - Lane A: 293T Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:111 kDa Observed band size:170 kDa](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-201-Western-Blot-NBP2-89890-img0003.jpg "Western Blot: ORP150/HSP12A Antibody (201) [NBP2-89890] - Lane A: 293T Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:111 kDa Observed band size:170 kDa")
![Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody (201) [NBP2-89890] - Staining of ORP150 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ORP150 monoclonal antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-201-Immunocytochemistry-Immunofluorescence-NBP2-89890-img0005.jpg)
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (201) [NBP2-89890] - Staining of human HSP12A in human colon carcinoma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-201-Immunohistochemistry-Paraffin-NBP2-89890-img0002.jpg)
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (201) [NBP2-89890] - Staining of human HSP12A in human cirrhosis with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-201-Immunohistochemistry-Paraffin-NBP2-89890-img0001.jpg)
![Immunoprecipitation: ORP150/HSP12A Antibody (201) [NBP2-89890] - Lane A:0.5 mg MCF-7 Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg Hela Whole Cell Lysate 2 uL anti-HSP12A rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-HSP12A rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 111 kDaObserved band size: 111 kDa](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-201-Immunoprecipitation-NBP2-89890-img0004.jpg)
![Western Blot: ORP150/HSP12A Antibody (6E3-2C3) [NBP2-59345] - Western Blot analysis of Rat Liver showing detection of ~170 kDa ORP150/HSP12A protein using Mouse Anti-ORP150/HSP12A Monoclonal Antibody, Clone 6E3-2C3 (NBP2-59345). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysate. Load: 20 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ORP150/HSP12A Monoclonal Antibody (NBP2-59345) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-6E3-2C3-Western-Blot-NBP2-59345-img0007.jpg "Western Blot: ORP150/HSP12A Antibody (6E3-2C3) [NBP2-59345] - Western Blot analysis of Rat Liver showing detection of ~170 kDa ORP150/HSP12A protein using Mouse Anti-ORP150/HSP12A Monoclonal Antibody, Clone 6E3-2C3 (NBP2-59345). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysate. Load: 20 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ORP150/HSP12A Monoclonal Antibody (NBP2-59345) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa.")
![Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody (6E3-2C3) [NBP2-59345] - Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP170 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-6E3-2C3-Immunocytochemistry-Immunofluorescence-NBP2-59345-img0002.jpg)
![Western Blot: ORP150/HSP12A Antibody (6E3-2C3) [NBP2-59345] - Analysis of Human HEK-293 cell lysate showing detection of ~170 kDa GRP170 protein using Mouse Anti-GRP170 Monoclonal Antibody, Clone 6E3-2C2. Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK-293 cell lysate. Load: 20 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-GRP170 Monoclonal Antibody at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa. Other Band(s): 100 kDa.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-6E3-2C3-Western-Blot-NBP2-59345-img0006.jpg)
![Western Blot: ORP150/HSP12A Antibody (6H7X2) [NBP3-15331] - Western blot analysis of extracts of various cell lines, using ORP150/HSP12A Rabbit mAb (NBP3-15331) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-6H7X2-Western-Blot-NBP3-15331-img0002.jpg "Western Blot: ORP150/HSP12A Antibody (6H7X2) [NBP3-15331] - Western blot analysis of extracts of various cell lines, using ORP150/HSP12A Rabbit mAb (NBP3-15331) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (6H7X2) [NBP3-15331] - Immunohistochemistry of paraffin-embedded rat pancreas using ORP150/HSP12A Rabbit mAb (NBP3-15331) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-6H7X2-Immunohistochemistry-Paraffin-NBP3-15331-img0001.jpg)
![Western Blot: ORP150/HSP12A Antibody (S04-4K7) [NBP3-19728] - Western blot detection of ORP150/HSP12A in 293T, MCF-7 using NBP3-19728. Predicted band size: 111kDa. Observed band size: 150kDa.](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-S04-4K7-Western-Blot-NBP3-19728-img0001.jpg "Western Blot: ORP150/HSP12A Antibody (S04-4K7) [NBP3-19728] - Western blot detection of ORP150/HSP12A in 293T, MCF-7 using NBP3-19728. Predicted band size: 111kDa. Observed band size: 150kDa.")
![Immunoprecipitation:ORP150/HSP12A AntibodyNBP3-30107] -Detection of human ORP150/HYOU1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ORP150/HYOU1 antibody (NBP3-30107 lot 1) used for IP at 1 µg per reaction. ORP150/HYOU1 was also immunoprecipitated by a second.inst a different epitope of ORP150/HYOU1 (BL19596). For blotting immunoprecipitated ORP150/HYOU1, NBP3-30107 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30107_rabbit-orp150-hsp12a-pab-3012202412262512.jpg "Immunoprecipitation:ORP150/HSP12A AntibodyNBP3-30107] -Detection of human ORP150/HYOU1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ORP150/HYOU1 antibody (NBP3-30107 lot 1) used for IP at 1 µg per reaction. ORP150/HYOU1 was also immunoprecipitated by a second.inst a different epitope of ORP150/HYOU1 (BL19596). For blotting immunoprecipitated ORP150/HYOU1, NBP3-30107 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP2-99781] - Immunochemical staining of human ORP150/HSP12A in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Immunohistochemistry-Paraffin-NBP2-99781-img0002.jpg "Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP2-99781] - Immunochemical staining of human ORP150/HSP12A in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody [NBP2-99781] - Immunochemical staining of human ORP150/HSP12A in human breast carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/ORP150-HSP12A-Antibody-Immunohistochemistry-Paraffin-NBP2-99781-img0001.jpg)