gp96/HSP90B1/GRP94 Antibody (9G10)

Images

 
Western Blot: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of 25 ug of Hela (lane 1), C6 (lane 2) and NIH-3T3 (lane 3) lysates.
Immunocytochemistry/ Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - GR94 in human primary fibroblasts, ER staining. Primary antibody incubation: 1:250 in 10% Serum in PBST (0.1% Triton) O/N 4C. ...read more
Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Normal biopsies of deparaffinized Mouse breast tissue.
Western Blot: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of 2-fold serial dilutions of HeLa cell lysate, starting at 10 ug, per well.
Immunocytochemistry/ Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) shows staining in C6 ...read more
Immunocytochemistry/ Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) shows staining in Hela ...read more
Immunocytochemistry/ Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) shows staining in NIH-3T3 ...read more
Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Normal biopsies of deparaffinized Mouse liver tissue.
Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Normal biopsies of deparaffinized Mouse lymph node.
Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of Glucose-Regulated Protein 94 (Grp94) in U2OS cells. Cells fixed with 4% paraformaldehyde were permeabilized with 0.1% Triton ...read more
Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Staining on poorly differentiated adenocarcinoma from paraffin-embedded human colon tissue sections.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, HaSpecies Glossary
Applications WB, ICC/IF, IHC, IP, ICC/IF
Clone
9G10
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

gp96/HSP90B1/GRP94 Antibody (9G10) Summary

Immunogen
Chick oviduct GRP94.
Localization
Endoplasmic reticulum
Marker
ER Stress Marker
Specificity
GRP94 (9G10)
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
HSP90B1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry
  • Immunocytochemistry/Immunofluorescence 1:250
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:20
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:1000
Application Notes
WB: Detects an approx. 108 kDa protein representing GRP94 in chick oviduct cytosol.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB300-619 in the following applications:

Publications
Read Publications using
NB300-619 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for gp96/HSP90B1/GRP94 Antibody (9G10)

  • 94 kDa glucose-regulated protein
  • ECGP
  • Endoplasmin
  • endothelial cell (HBMEC) glycoprotein
  • glucose regulated protein, 94 kDa
  • gp96
  • GP96ECGP
  • Grp94
  • GRP-94
  • GRP94gp96 homolog
  • Heat shock protein 90 kDa beta member 1
  • heat shock protein 90kDa beta (Grp94), member 1
  • HSP90B1
  • TRA1endoplasmin
  • tumor rejection antigen (gp96) 1
  • Tumor rejection antigen 1
  • Tumor rejection antigen-1 (gp96)

Background

Glucose-regulated protein 94, also known as Grp94 or gp96, is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. Grp94 and other resident soluble proteins of the ER such as members of the Ca(2+) binding protein subfamily (CaBP), CaBPI and CaBP2 as well as calreticulin, possess the COOH-terminal tetrapeptide Lys-Asp-Glu-Leu (KDEL) which is a sorting signal that is thought to lead to the retention of these proteins in the pre-Golgi compartments (1). Grp94 expression is upregulated by stress conditions such as lucose starvation and heat shock, which promote protein misfolding or unfolding (2). In addition to a homeostatic role in protein folding and assembly, Grp94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells (3,4). Grp94 and Hsp90 share high sequence identity and presumably identical adenosine nucleotide-dependent modes of regulation. Earlier data suggests that Hsp90 and Grp94 may differ in their nucleotide binding properties. The N-terminal domain of eukaryotic Hsp90 proteins contains a conserved adenosine nucleotide binding pocket which also serves as the inding site for the Hsp90 inhibitors geldanamycin and radicicol. However, the molecular basis for adenosine nucleotide-dependent regulation of Grp94remains to be established. Recent data has entified a ligand dependent regulation of Grp94 function and suggest a model whereby Grp94 function is regulated through a ligand-dependent conversion of Grp94 from an inactive to an active conformation (5, 6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Bv, Ch, Ha
Applications: WB, ICC/IF, IHC, IP, ICC/IF

Publications for gp96/HSP90B1/GRP94 Antibody (NB300-619)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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(1)
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Reviews for gp96/HSP90B1/GRP94 Antibody (NB300-619) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB300-619:
Filter by Applications
ICC
(1)
WB
(1)
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Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
Immunocytochemistry gp96/HSP90B1/GRP94 NB300-619
Enlarge
4
reviewed by:
Marina Villar
ICC Human 11/22/2020
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman primary fibroblast
SpeciesHuman
Lottl277703

Comments

CommentsPFA 4% 15 min fixed. Primary ab incubation: 1:250 in 10% Serum in PBST (0.1% Triton) O/N 4ºC. Secondary: Donkey anti-rat 647 1:500 2h RT.
Western Blot gp96/HSP90B1/GRP94 NB300-619
Enlarge
5
reviewed by:
Marina Villar
WB Human 10/25/2020
View

Summary

ApplicationWestern Blot
Sample TestedHuman primary fibroblast
SpeciesHuman
Lottl277703

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for gp96/HSP90B1/GRP94 Antibody (NB300-619) (0)

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
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Marina Villar
11/22/2020
Application: ICC
Species: Human

Marina Villar
10/25/2020
Application: WB
Species: Human

Bioinformatics

Gene Symbol HSP90B1
Entrez
Uniprot