Calreticulin Antibody

Images

 
Genetic Strategies: Knockdown Validated: Calreticulin Antibody [NB600-101] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout (KO) HeLa cell ...read more
Western Blot: Calreticulin Antibody [NB600-101] - Human kidney lysate.
Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody [NB600-101] - Immunofluorescence staining of Calreticulin in HCT15 colon cancer cells using NB600-101. Secondary antibody was conjugated with Alexa Fluor ...read more
Immunohistochemistry-Paraffin: Calreticulin Antibody [NB600-101] - Analysis of a FFPE tissue section of human thyroid gland using 1:50 dilution of Calreticulin antibody. The signal was developed using HRP-DAB based ...read more
Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody [NB600-101] - The Calreticulin antibody NB600-101 was tested in HeLa cells at a 1:250 dilution against DyLight 488 (Green). Alpha-tubulin and nuclei were ...read more
Immunohistochemistry-Paraffin: Calreticulin Antibody [NB600-101] - Analysis of a FFPE tissue section of human thyroid gland using 1:50 dilution of Calreticulin antibody. The signal was developed using HRP-DAB based ...read more
Simple Western: Calreticulin Antibody [NB600-101] - Lane view shows a specific band for Calreticulin in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ha, PmSpecies Glossary
Applications WB, Simple Western, DB, EM, Flow, ICC/IF, IHC, IHC-P, IP, PA, B/N, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Calreticulin Antibody Summary

Immunogen
A fusion protein to mouse Calreticulin [Uniprot: P14211]
Localization
Calreticulin staining is localized to the ER in most cell types and under most conditions.
Marker
Endoplasmic Reticulum Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CALR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:50
  • Dot Blot
  • Electron Microscopy
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 -1:200
  • Immunoprecipitation
  • Protein Array
  • Block/Neutralize
  • Knockdown Validated 1:1500
Application Notes
In Western blot, a band is observed at ~55 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Protein Array (PMID: 17897946), and Immunoprecipitation (PMID: 15161937) were reported in scientific literature. Use in FLOW reported in scientific literature (PMID: 19171874). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
Calreticulin Overexpression Lysate
Calreticulin Knockout HeLa Cell Lysate
Publications
Read Publications using
NB600-101 in the following applications:

  • B/N
    1 publication
  • DB
    1 publication
  • EM
    1 publication
  • 1 publication
  • 1 publication
  • 9 publications
  • IHC
    3 publications
  • IP
    1 publication
  • PA
    1 publication
  • WB
    11 publications

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Calreticulin Antibody

  • Autoantigen Ro
  • CALR
  • Calregulin
  • Calreticulin
  • cC1qR
  • CRP55
  • CRT
  • CRTC
  • Endoplasmic reticulum resident protein 60
  • ERp60
  • FLJ26680
  • grp60
  • RO
  • Sicca syndrome antigen A (autoantigen Ro; calreticulin)
  • SSA
  • SSAHACBP
  • Vasostatin

Background

Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum (ER). It is also a molecular chaperone and is involved in protein folding events in the ER. Calreticulin has also been shown to interact with the cytoskeleton and to be involved in the regulation of gene expression. Calreticulin may play a role in cellular proliferation including its apparent activity in the proliferation of certain viruses within mammalian host cells. (2-3) Recently it has been shown that calreticulin is induced in response to various types of cell stress including amino acid deprivation. (1) Close interconnections among protein synthesis, gene expression and calcium signaling have been observed by many researchers in recent years. Calreticulin might be centrally located and crucially participate in the coordination of these functions by the cell.(2-3)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calreticulin Antibody (NB600-101)(24)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Hamster, Primate.

We have publications tested in 10 applications: B/N, DB, EM, FLOW, Flow, ICC/IF, IHC, IP, PA, WB.


Filter By Application
B/N
(1)
DB
(1)
EM
(1)
FLOW
(1)
Flow
(1)
ICC/IF
(9)
IHC
(3)
IP
(1)
PA
(1)
WB
(11)
All Applications
Filter By Species
Human
(6)
Mouse
(4)
Rat
(6)
Bovine
(1)
Hamster
(1)
Primate
(1)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NB600-101 Applications Species
Roberti MP, Yonekura S, Duong CPM et al. Chemotherapy-induced ileal crypt apoptosis and the ileal microbiome shape immunosurveillance and prognosis of proximal colon cancer Nat. Med. May 25 2020 [PMID: 32451498] (Mouse) Mouse
Musante L, Bontha Sv, La Salvia S et Al. Rigorous characterization of urinary extracellular vesicles (uEVs) in the low centrifugation pellet - a neglected source for uEVs Sci Rep Feb 28 2020 [PMID: 32111925] (WB, Human) WB Human
Lekszas C, Foresti O, Raote I et Al. Biallelic TANGO1 mutations cause a novel syndromal disease due to hampered cellular collagen secretion Elife Feb 26 2020 [PMID: 32101163] (ICC/IF, Human) ICC/IF Human
Dong X, Wu W, Ma L et al. Collectin-11 Is an Important Modulator of Retinal Pigment Epithelial Cell Phagocytosis and Cytokine Production J Innate Immun 2017 Aug 04 [PMID: 28772263] (Flow, Mouse) Flow Mouse
Yamada HY, Kumar G, Zhang Y et al. Systemic chromosome instability in Shugoshin-1 mice resulted in compromised glutathione pathway, activation of Wnt signaling and defects in immune system in the lung. Oncogenesis. Aug 16 2016 [PMID: 27526110] (WB, IHC) WB, IHC
Teng CY, van Oers MM, Wu TY et al. Additive effect of calreticulin and translation initiation factor eIF4E on secreted protein production in the baculovirus expression system. Appl Microbiol Biotechnol 2013 Jul 31 [PMID: 23900798] (WB) WB
Teng CY, Chang SL, Tsai MF, Wu TY. alpha-Synuclein and beta-synuclein enhance secretion protein production in baculovirus expression vector system Appl Microbiol Biotechnol 2013 Jan 12 [PMID: 23314197] (WB) WB
Teng CY, Chang SL, van Oers MM, Wu TY. Enhanced Protein Secretion From Insect Cells by Co-Expression of the Chaperone Calreticulin and Translation Initiation Factor eIF4E. Mol Biotechnol. 2012 May 4. [PMID: 22555850] (WB) WB
Bazwinsky-Wutschke I, Wolgast S, Muhlbauer E, Peschke E. Distribution patterns of calcium-binding proteins in pancreatic tissue of non-diabetic as well as type 2 diabetic rats and in rat insulinoma beta-cells (INS-1). Histochem Cell Biol 2010 Aug [PMID: 20607274] (Rat) Rat
Anderson DJ et al. Recruitment of functionally distinct membrane proteins to chromatin mediates nuclear envelope formation in vivo. J Cell Biol;186(2):183-91. 2009 Jul 27. [PMID: 19620630] (WB, ICC/IF) WB, ICC/IF
Show All 24 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calreticulin Antibody (NB600-101). (Showing 1 - 3 of 3 FAQs).

  1. We are looking to buy an anti-calreticulin antibody that would work for WB and IP. Your reference NB 600-101 wouls correspond, but we need it to work on proteins with altered C terminus. Could you please let us know whether the immunizing peptide for this antibody is located N-terminal to AA #364?
    • Yes, our product NB600-101 was raised against an immunogen that falls between amino acids 150-350 of the mouse Calreticulin protein.
  2. I would like to know how the NB600-101 Calreticulin Antibody was used in the neutralization/blocking application. Do you have protocol details for a particular cell type etc?
    • NB600-101 was validated for B/N via a publication. We do not have a specific protocol available; however, I do have the link to that publication that your customer may find helpful. NCBI site
  3. Is this antibody (NB600-101) suitable for immunolabeling in Tokuyasu criosections?
    • This product has not been tested in Tokuyasu Cryosections but is validated in IHC/ICC/IF. We do assume it should work for you.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-101AF350
Alexa Fluor 405 NB600-101AF405
Alexa Fluor 488 NB600-101AF488
Alexa Fluor 532 NB600-101AF532
Alexa Fluor 594 NB600-101AF594
Alexa Fluor 647 NB600-101AF647
Alexa Fluor 700 NB600-101AF700
Alexa Fluor 750 NB600-101AF750
DyLight 350 NB600-101UV
DyLight 405 NB600-101V
DyLight 488 NB600-101G
DyLight 550 NB600-101R
DyLight 594 NB600-101DL594
DyLight 650 NB600-101C
DyLight 680 NB600-101FR
DyLight 755 NB600-101IR

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Blogs on Calreticulin.

Calreticulin - ER chaperone involved in calcium homeostasis and protein quality control
Calreticulin is a calcium-dependent ER chaperone, involved in protein folding, maturation, and cellular localization. Calreticulin is a highly conserved 48 kDa protein encoded by the CALR gene. Calreticulin and its homolog calnexin regulate the fo...  Read full blog post.

Calnexin - an ER chaperone that folds the cell's glycoproteins
Calnexin is an abundant 90kDa chaperone protein that resides in the membrane of the endoplasmic reticulum. Calnexin and the related calreticulin protein function together to ensure the proper folding of glycoproteins. By binding to partially folded...  Read full blog post.

Calreticulin: a Multiprocess Calcium Buffering Chaperone
Calreticulin is a Calcium binding chaperone that has multiple functions both inside and outside the endoplasmic reticulum. Calreticulin is involved in the quality control of newly synthesized proteins and glycoproteins, interacting with various other...  Read full blog post.

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Bioinformatics

Gene Symbol CALR
Entrez
Uniprot