Western Blot: Calreticulin Antibody [NB600-101] - Human kidney lysate.
Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody [NB600-101] - Immunofluorescence staining of Calreticulin in HCT15 colon cancer cells using NB600-101. Secondary antibody was an Alexa Fluor 488. Photo ...read more
Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody [NB600-101] - The Calreticulin antibody NB600-101 was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were ...read more
Simple Western: Calreticulin Antibody [NB600-101] - Simple Western lane view shows a specific band for Calreticulin in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 ...read more
Hu, Mu, Rt, Bv, Ha, PmSpecies Glossary
WB, Simple Western, B/N, DB, EM, ICC/IF, IHC, IP, PA
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
This Calreticulin antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Western blot, where a band is observed at ~55 kDa. Electron Microscopy, Blocking/Neutralizing, Dot Blot, Immunoprecipitation and Protein Array were reported in scientific literature.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Human, bovine, rat and mouse calreticulin. Others not tested.
Alternate Names for Calreticulin Antibody
Endoplasmic reticulum resident protein 60
Sicca syndrome antigen A (autoantigen Ro; calreticulin)
Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum (ER). It is also a molecular chaperone and is involved in protein folding events in the ER. Calreticulin has also been shown to interact with the cytoskeleton and to be involved in the regulation of gene expression. Calreticulin may play a role in cellular proliferation including its apparent activity in the proliferation of certain viruses within mammalian host cells. (2-3) Recently it has been shown that calreticulin is induced in response to various types of cell stress including amino acid deprivation. (1) Close interconnections among protein synthesis, gene expression and calcium signaling have been observed by many researchers in recent years. Calreticulin might be centrally located and crucially participate in the coordination of these functions by the cell.(2-3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Calreticulin Antibody (NB600-101). (Showing 1 - 2 of 2 FAQs).
We are looking to buy an anti-calreticulin antibody that would work for WB and IP. Your reference NB 600-101 wouls correspond, but we need it to work on proteins with altered C terminus. Could you please let us know whether the immunizing peptide for this antibody is located N-terminal to AA #364?
Yes, our product NB600-101 was raised against an immunogen that falls between amino acids 150-350 of the mouse Calreticulin protein.
I would like to know how the NB600-101 Calreticulin Antibody was used in the neutralization/blocking application. Do you have protocol details for a particular cell type etc?
NB600-101 was validated for B/N via a publication. We do not have a specific protocol available; however, I do have the link to that publication that your customer may find helpful. NCBI site