Calreticulin Antibody - BSA Free

Images

 
Genetic Strategies: Knockdown Validated: Calreticulin Antibody [NB600-101] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout (KO) HeLa cell ...read more
Western Blot: Calreticulin Antibody [NB600-101] - Human kidney lysate.
Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody [NB600-101] - Immunofluorescence staining of Calreticulin in HCT15 colon cancer cells using NB600-101. Secondary antibody was conjugated with Alexa Fluor ...read more
Immunohistochemistry-Paraffin: Calreticulin Antibody [NB600-101] - Analysis of a FFPE tissue section of human thyroid gland using 1:50 dilution of Calreticulin antibody. The signal was developed using HRP-DAB based ...read more
Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody [NB600-101] - The Calreticulin antibody NB600-101 was tested in HeLa cells at a 1:250 dilution against DyLight 488 (Green). Alpha-tubulin and nuclei were ...read more
Immunohistochemistry-Paraffin: Calreticulin Antibody [NB600-101] - Analysis of a FFPE tissue section of human thyroid gland using 1:50 dilution of Calreticulin antibody. The signal was developed using HRP-DAB based ...read more
Simple Western: Calreticulin Antibody [NB600-101] - Lane view shows a specific band for Calreticulin in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ha, PmSpecies Glossary
Applications WB, Simple Western, DB, EM, Flow, ICC/IF, IHC, IHC-P, IP, PA, B/N, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Calreticulin Antibody - BSA Free Summary

Immunogen
Calreticulin Antibody was developed against a fusion protein to mouse Calreticulin [Uniprot: P14211]
Localization
Calreticulin staining is localized to the ER in most cell types and under most conditions.
Marker
Endoplasmic Reticulum Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CALR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize
  • Dot Blot
  • Electron Microscopy
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 -1:200
  • Immunoprecipitation
  • Knockdown Validated 1:1500
  • Protein Array
  • Simple Western 1:50
  • Western Blot 1:1000
Application Notes
In Western blot, a band is observed at ~55 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Protein Array (PMID: 17897946), and Immunoprecipitation (PMID: 15161937) were reported in scientific literature. Use in FLOW reported in scientific literature (PMID: 19171874). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Calreticulin Overexpression Lysate
Publications
Read Publications using
NB600-101 in the following applications:

  • B/N
    1 publication
  • DB
    1 publication
  • EM
    1 publication
  • 1 publication
  • 1 publication
  • 9 publications
  • IHC
    3 publications
  • IP
    1 publication
  • PA
    1 publication
  • WB
    10 publications

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Calreticulin Antibody - BSA Free

  • Autoantigen Ro
  • CALR
  • Calregulin
  • Calreticulin
  • cC1qR
  • CRP55
  • CRT
  • Endoplasmic reticulum resident protein 60
  • epididymis secretory sperm binding protein Li 99n
  • ERp60
  • FLJ26680
  • grp60
  • HACBP
  • HEL-S-99n
  • RO
  • Sicca syndrome antigen A (autoantigen Ro; calreticulin)
  • SSA
  • Vasostatin

Background

Calreticulin, also called CALR, is an endoplasmic reticulum (ER) protein that functions as a calcium (Ca2+) buffering molecular chaperone (1-3). Together with calnexin and ERp57, calreticulin has a critical role in protein folding and quality control of newly synthesized glycoproteins (1-3). Calreticulin has many diverse functions beyond Ca2+ binding, including cell adhesion, lectin binding, apoptosis, nuclear transport, antigen presentation, and immune response (1-3). Specifically, calreticulin is part of the peptide-loading complex that resides on the ER membrane and is responsible for antigen loading onto MHC class I molecules (1-3). Calreticulin protein has a theoretical molecular weight of 46 kDa and is 417 amino acids (aa) in length (1-3). The protein has three main domains: the N-terminal lectin-like domain, the proline-rich P-domain, and the acidic C-domain which is followed by an ER-retention KDEL signal sequence (1-3).

Given its role in multiple biological processes, it makes sense that calreticulin is implicated in both healthy and disease states. Studies have found that calreticulin mutations were present in patients with myeloproliferative neoplasms (MFN) and essential thrombocythaemia (ET) (4). Calreticulin expression is typically upregulated in most cancer lines, however it is downregulated in some tissues including cervical carcinomas, prostate cancer, and human colon adenocarcinoma (3). High expression of calreticulin on cancer cells is related to tumor cell phagocytosis and is often correlated with, and counteracted by, elevated CD47 expression to prevent cancer cell phagocytosis (3). Calreticulin mutations can serve as a major diagnostic biomarker for MFN and ET and additionally the calreticulin gene may be a potential target for cancer therapeutics (3,4).

References

1. Michalak, M., Groenendyk, J., Szabo, E., Gold, L. I., & Opas, M. (2009). Calreticulin, a multi-process calcium-buffering chaperone of the endoplasmic reticulum. The Biochemical Journal. https://doi.org/10.1042/BJ20081847

2. Fucikova, J., Spisek, R., Kroemer, G., & Galluzzi, L. (2021). Calreticulin and cancer. Cell Research. https://doi.org/10.1038/s41422-020-0383-9

3. Sun, J., Mu, H., Dai, K., & Yi, L. (2017). Calreticulin: a potential anti-cancer therapeutic target. Die Pharmazie. https://doi.org/10.1691/ph.2017.7031

4. Prins, D., Gonzalez Arias, C., Klampfl, T., Grinfeld, J., & Green, A. R. (2020). Mutant Calreticulin in the Myeloproliferative Neoplasms. HemaSphere. https://doi.org/10.1097/HS9.0000000000000333

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calreticulin Antibody (NB600-101)(26)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Hamster, Primate.

We have publications tested in 10 applications: B/N, DB, EM, FLOW, Flow, ICC/IF, IHC, IP, PA, WB.


Filter By Application
B/N
(1)
DB
(1)
EM
(1)
FLOW
(1)
Flow
(1)
ICC/IF
(9)
IHC
(3)
IP
(1)
PA
(1)
WB
(10)
All Applications
Filter By Species
Human
(7)
Mouse
(4)
Rat
(6)
Bovine
(1)
Hamster
(1)
Primate
(1)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using NB600-101 Applications Species
Zheng C, Sui B, Zhang X et al. Apoptotic vesicles restore liver macrophage homeostasis to counteract type 2 diabetes Journal of Extracellular Vesicles May 1 2021 [PMID: 34084287] (Human) Human
Harada K, Matsuoka H, Toyohira Y et al. Mechanisms for establishment of GABA signaling in adrenal medullary chromaffin cells Journal of neurochemistry Mar 11 2021 [PMID: 33704788]
Roberti MP, Yonekura S, Duong CPM et al. Chemotherapy-induced ileal crypt apoptosis and the ileal microbiome shape immunosurveillance and prognosis of proximal colon cancer Nat. Med. May 25 2020 [PMID: 32451498] (Mouse) Mouse
Lekszas C, Foresti O, Raote I et Al. Biallelic TANGO1 mutations cause a novel syndromal disease due to hampered cellular collagen secretion Elife Feb 26 2020 [PMID: 32101163] (ICC/IF, Human) ICC/IF Human
Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods Jan 11 2018 [PMID: 30377371] (Human)

Details:
Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).
Human
Dong X, Wu W, Ma L et al. Collectin-11 Is an Important Modulator of Retinal Pigment Epithelial Cell Phagocytosis and Cytokine Production J Innate Immun 2017 Aug 04 [PMID: 28772263] (Flow, Mouse) Flow Mouse
Yamada HY, Kumar G, Zhang Y et al. Systemic chromosome instability in Shugoshin-1 mice resulted in compromised glutathione pathway, activation of Wnt signaling and defects in immune system in the lung. Oncogenesis. Aug 16 2016 [PMID: 27526110] (WB, IHC) WB, IHC
Teng CY, van Oers MM, Wu TY et al. Additive effect of calreticulin and translation initiation factor eIF4E on secreted protein production in the baculovirus expression system. Appl Microbiol Biotechnol 2013 Jul 31 [PMID: 23900798] (WB) WB
Teng CY, Chang SL, Tsai MF, Wu TY. alpha-Synuclein and beta-synuclein enhance secretion protein production in baculovirus expression vector system Appl Microbiol Biotechnol 2013 Jan 12 [PMID: 23314197] (WB) WB
Teng CY, Chang SL, van Oers MM, Wu TY. Enhanced Protein Secretion From Insect Cells by Co-Expression of the Chaperone Calreticulin and Translation Initiation Factor eIF4E. Mol Biotechnol. 2012 May 4. [PMID: 22555850] (WB) WB
Show All 26 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calreticulin Antibody (NB600-101). (Showing 1 - 3 of 3 FAQs).

  1. We are looking to buy an anti-calreticulin antibody that would work for WB and IP. Your reference NB 600-101 wouls correspond, but we need it to work on proteins with altered C terminus. Could you please let us know whether the immunizing peptide for this antibody is located N-terminal to AA #364?
    • Yes, our product NB600-101 was raised against an immunogen that falls between amino acids 150-350 of the mouse Calreticulin protein.
  2. I would like to know how the NB600-101 Calreticulin Antibody was used in the neutralization/blocking application. Do you have protocol details for a particular cell type etc?
    • NB600-101 was validated for B/N via a publication. We do not have a specific protocol available; however, I do have the link to that publication that your customer may find helpful. NCBI site
  3. Is this antibody (NB600-101) suitable for immunolabeling in Tokuyasu criosections?
    • This product has not been tested in Tokuyasu Cryosections but is validated in IHC/ICC/IF. We do assume it should work for you.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Calreticulin.

Calreticulin - ER chaperone involved in calcium homeostasis and protein quality control
Calreticulin is a calcium-dependent ER chaperone, involved in protein folding, maturation, and cellular localization. Calreticulin is a highly conserved 48 kDa protein encoded by the CALR gene. Calreticulin and its homolog calnexin regulate the fo...  Read full blog post.

Calnexin - an ER chaperone that folds the cell's glycoproteins
Calnexin is an abundant 90kDa chaperone protein that resides in the membrane of the endoplasmic reticulum. Calnexin and the related calreticulin protein function together to ensure the proper folding of glycoproteins. By binding to partially folded...  Read full blog post.

Calreticulin: a Multiprocess Calcium Buffering Chaperone
Calreticulin is a Calcium binding chaperone that has multiple functions both inside and outside the endoplasmic reticulum. Calreticulin is involved in the quality control of newly synthesized proteins and glycoproteins, interacting with various other ...  Read full blog post.

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Bioinformatics

Gene Symbol CALR
Entrez
Uniprot