Calnexin Antibody

Western Blot: Calnexin Antibody [NB100-1965] - A dilution of 1/2000 used in TBS with 0.05% Tween-20. Bands visualized by ECL using goat anti-rabbit HRP at 1:2000.

Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1965] - Antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).

Immunohistochemistry: Calnexin Antibody [NB100-1965] - IHC analysis of CANX in mouse prostate using DAB with hematoxylin counterstain.

Western Blot: Calnexin Antibody [NB100-1965] - WB analysis of CANX in cell lysates as noted.

Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1965] - Calnexin antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Simple Western: Calnexin Antibody [NB100-1965] - Simple Western lane view shows a specific band for Calnexin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Po, Av, Bv, Ch, Dr, GP, Rb, Sh, Xp, Ze, Ca
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Calnexin Antibody Summary

• Immunogen: A synthetic peptide made to an internal region of the canine Calnexin protein (within residues 25-100). [Swiss-Prot P24643]
• Localization: Endoplasmic reticulum
• Marker: Endoplasmic Reticulum Membrane Marker
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CANX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/ml
  • Simple Western 1:25
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:40
  • Immunohistochemistry-Paraffin 1:40
  • Immunoprecipitation 1:100
• Application Notes: This Calnexin antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, Immunoprecipitation and Western Blot. In Western blot a band is observed approx. 97 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse, rat, Xenopus laevis, bovine, chicken, Drosophilia melanogaster, guinea pig, pig, quail, rabbit, zebrafish and sheep. Predicted to react with dog based on 100% sequence homology.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.5% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Calnexin Antibody

• calnexin
• CNX
• IP90FLJ26570
• Major histocompatibility complex class I antigen-binding protein p88
• P90

Background

Calnexin is a chaperone protein that assists in protein folding and quality control. Calnexin retains unassembled or unfolded N-linked glycoproteins in the endoplasmic reticulum and ensures that only properly assembled and folded proteins proceed down the secretory pathway. Calnexin antibodies can be used as endoplasmic reticulum markers (ER markers) in immunostaining experiments

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calnexin Antibody (NB100-1965)(17)

Publications using NB100-1965

• Xu Y, Xu Y, Zhu Y et al. Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis Mol. Ther. 2019 Sep 12 [PMID: 31604677]
• Jo Y, Hamilton JS, Hwang S et al. Schnyder corneal dystrophy-associated UBIAD1 inhibits ER-associated degradation of HMG CoA reductase in mice Elife Feb 20 2019 [PMID: 30785396] (WB, Mouse)
• Tiedemann K, Sadvakassova G, Mikolajewicz N et al. Exosomal Release of L-Plastin by Breast Cancer Cells Facilitates Metastatic Bone Osteolysis Transl Oncol Dec 20 2018 [PMID: 30583289] (WB, Human)
• Gao HN, Guo HY, Zhang H et al. Yak milk-derived exosomes promote proliferation of intestinal epithelial cells in hypoxic environment J. Dairy Sci. Dec 20 2018 [PMID: 30580945] (WB, Bovine)
• Qi X, Schmiege P, Coutavas E et al. Two Patched molecules engage distinct sites on Hedgehog yielding a signaling-competent complex Science Aug 23 2018 [PMID: 30139912] (WB, Human)
• Qi X, Schmiege P, Coutavas E et al. Structures of human Patched and its complex with native palmitoylated sonic hedgehog. Nature. Jul 11 2018 [PMID: 29995851] (WB, Human)
• Borgna V, Villegas J, Burzio VA et al. Mitochondrial ASncmtRNA-1 and ASncmtRNA-2 as potent targets to inhibit tumor growth and metastasis in the RenCa murine renal adenocarcinoma model Oncotarget 2017 Jul 04 [PMID: 28620146] (WB, Mouse)
• Xu Y, Li F, Zalzala M et al. Farnesoid X receptor activation increases reverse cholesterol transport by modulating bile acid composition and cholesterol absorption in mice Hepatology 2016 Jun 30 [PMID: 27359351]
• Li N, Subrahmanyan L, Smith E et al. Mutations in the Histone Modifier PRDM6 Are Associated with Isolated Nonsyndromic Patent Ductus Arteriosus. Am. J. Hum. Genet. Jun 2 2016 [PMID: 27181681] (ICC/IF, Human)
• Smagris E, Gilyard S, BasuRay S et al. Inactivation of Tm6sf2, a Gene Defective in Fatty Liver Disease, Impairs Lipidation but Not Secretion of Very Low Density Lipoproteins. J Biol Chem 2016 May 13 [PMID: 27013658]
• Huynh N, VonMoss L, Smith D et al. Characterization of Regulatory Extracellular Vesicles from Osteoclasts. J Dent Res 2016 Jun [PMID: 26908631]
• Riemersma M, Sandrock J, Boltje TJ et al. Disease mutations in CMP-sialic acid transporter SLC35A1 result in abnormal alpha-dystroglycan O-mannosylation, independent from sialic acid. Hum. Mol. Genet. 2014 Dec 30 [PMID: 25552652] (WB, Human)
• Saeki T, Sato K, Ito S et al. Importance of uncharged polar residues and proline in the proximal two-thirds (Pro107-Ser128) of the highly conserved region of mouse ileal Na+-dependent bile acid transporter, Slc10a2, in transport activity and cellular expression. BMC Physiol 2013 Feb 4 [PMID: 23374508] (WB, Porcine)
• Yuen MY, Webb SE, Chan CM et al. Characterization of Ca(2+) signaling in the external yolk syncytial layer during the late blastula and early gastrula periods of zebrafish development Biochim Biophys Acta 2012 Nov 8 [PMID: 23142640] (IHC, ICC/IF, Zebrafish)
• Tian E, Hoffman MP, Ten Hagen KG. O-glycosylation modulates integrin and FGF signalling by influencing the secretion of basement membrane components. Nat Commun 3:869. 2012 May 29. [PMID: 22643896] (ICC/IF, Mouse)
• Corbel S, Sampath S, Schmedt C, Zhang Q COMPOSITIONS, METHODS, AND THERAPEUTIC USES RELATED TO FUSOGENIC PROTEIN MINION United States Patent Application 20190263899 2019 Aug 29
• McFarlane MR, Cantoria MJ, Linden AG et al. Scap is required for sterol synthesis and crypt growth in intestinal mucosa J. Lipid Res. 2015 Apr 20 [PMID: 25896350] (WB, Mouse)

Review for Calnexin Antibody (NB100-1965) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Elizabeth Soberg WB Mouse 06/13/2018

Summary

• Application: Western Blot
• Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line
• Species: Mouse
• Lot: C-3

Comments

• Comments: 20 ug of whole cell lysate and exosomal lysate were loaded.
  Primary was used @ 2 ug/mL.
  Donkey anti-rabbit-HRP secondary was used @ 1:5000.
  Entry level (ug sensitivity) ECL reagents were used.

Bioinformatics

• Gene Symbol: CANX
• Entrez:
  • 29144(Rat)
• Uniprot:
  • P24643(Canine)