Calnexin Antibody


Western Blot: Calnexin Antibody [NB100-1965] - A dilution of 1/2000 used in TBS with 0.05% Tween-20. Bands visualized by ECL using goat anti-rabbit HRP at 1:2000.
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody [NB100-1965] - Antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
Immunohistochemistry: Calnexin Antibody [NB100-1965] - IHC analysis of CANX in mouse prostate using DAB with hematoxylin counterstain.
Western Blot: Calnexin Antibody [NB100-1965] - WB analysis of CANX in cell lysates as noted.
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody [NB100-1965] - Calnexin antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and more
Simple Western: Calnexin Antibody [NB100-1965] - Simple Western lane view shows a specific band for Calnexin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa more

Product Details

Reactivity Hu, Mu, Rt, Po, Av, Bv, Ch, Dr, GP, Rb, Sh, Xp, Ze, CaSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
1 mg/ml

Calnexin Antibody Summary

Calnexin is a chaperone protein that assists in protein folding and quality control. Calnexin retains unassembled or unfolded N-linked glycoproteins in the endoplasmic reticulum and ensures that only properly assembled and folded proteins proceed down the secretory pathway. Calnexin antibodies can be used as endoplasmic reticulum markers (ER markers) in immunostaining experiments.
A synthetic peptide made to an internal region of the canine Calnexin protein (within residues 25-100). [Swiss-Prot P24643]
Endoplasmic reticulum
Endoplasmic Reticulum Membrane Marker
Predicted Species
Canine (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 2 ug/ml
  • Simple Western 1:25
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:40
  • Immunohistochemistry-Paraffin 1:40
  • Immunoprecipitation 1:100
Application Notes
This Calnexin antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, Immunoprecipitation and Western Blot. In Western blot a band is observed approx. 97 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NB100-1965 in the following applications:

  • 3 publications
  • IHC
    1 publication
  • WB
    3 publications

Reactivity Notes

Human, mouse, rat, Xenopus laevis, bovine, chicken, Drosophilia melanogaster, guinea pig, pig, quail, rabbit, zebrafish and sheep. Predicted to react with dog based on 100% sequence homology.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.5% Sodium Azide
1 mg/ml
Immunogen affinity purified

Alternate Names for Calnexin Antibody

  • calnexin
  • CNX
  • IP90FLJ26570
  • Major histocompatibility complex class I antigen-binding protein p88
  • P90


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC, IP
Species: Hu, Mu, Rt, Po, Av, Bv, Ch, Dr, GP, Rb, Sh, Xp, Ze, Ca
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP

Publications for Calnexin Antibody (NB100-1965)(9)

We have publications tested in 4 confirmed species: Human, Mouse, Porcine, Zebrafish.

We have publications tested in 3 applications: ICC/IF, IHC, WB.

Filter By Application
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Showing Publications 1 - 9 of 9.
Publications using NB100-1965 Applications Species
Xu Y, Li F, Zalzala M et al. Farnesoid X receptor activation increases reverse cholesterol transport by modulating bile acid composition and cholesterol absorption in mice Hepatology 2016 Jun 30 [PMID: 27359351]
Li N, Subrahmanyan L, Smith E et al. Mutations in the Histone Modifier PRDM6 Are Associated with Isolated Nonsyndromic Patent Ductus Arteriosus. Am. J. Hum. Genet. Jun 2 2016 [PMID: 27181681] (ICC/IF, Human) ICC/IF Human
Smagris E, Gilyard S, BasuRay S et al. Inactivation of Tm6sf2, a Gene Defective in Fatty Liver Disease, Impairs Lipidation but Not Secretion of Very Low Density Lipoproteins. J Biol Chem 2016 May 13 [PMID: 27013658]
Huynh N, VonMoss L, Smith D et al. Characterization of Regulatory Extracellular Vesicles from Osteoclasts. J Dent Res 2016 Jun [PMID: 26908631]
McFarlane MR, Cantoria MJ, Linden AG et al. Scap is required for sterol synthesis and crypt growth in intestinal mucosa J. Lipid Res. 2015 Apr 20 [PMID: 25896350] (WB, Mouse) WB Mouse
Riemersma M, Sandrock J, Boltje TJ et al. Disease mutations in CMP-sialic acid transporter SLC35A1 result in abnormal alpha-dystroglycan O-mannosylation, independent from sialic acid. Hum. Mol. Genet. 2014 Dec 30 [PMID: 25552652] (WB, Human) WB Human
Saeki T, Sato K, Ito S et al. Importance of uncharged polar residues and proline in the proximal two-thirds (Pro107-Ser128) of the highly conserved region of mouse ileal Na+-dependent bile acid transporter, Slc10a2, in transport activity and cellular expression. BMC Physiol 2013 Feb 4 [PMID: 23374508] (WB, Porcine) WB Porcine
Yuen MY, Webb SE, Chan CM et al. Characterization of Ca(2+) signaling in the external yolk syncytial layer during the late blastula and early gastrula periods of zebrafish development Biochim Biophys Acta 2012 Nov 8 [PMID: 23142640] (IHC, ICC/IF, Zebrafish) IHC, ICC/IF Zebrafish
Tian E, Hoffman MP, Ten Hagen KG. O-glycosylation modulates integrin and FGF signalling by influencing the secretion of basement membrane components. Nat Commun 3:869. 2012 May 29. [PMID: 22643896] (ICC/IF, Mouse) ICC/IF Mouse

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calnexin Antibody (NB100-1965) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-1965AF405
Alexa Fluor 488 Labeled NB100-1965AF488
Alexa Fluor 647 Labeled NB100-1965AF647
Alexa Fluor 700 Labeled NB100-1965AF700
Biotin Labeled NB100-1965B
DyLight 405 Labeled NB100-1965V
DyLight 488 Labeled NB100-1965G
DyLight 550 Labeled NB100-1965R
DyLight 650 Labeled NB100-1965C
FITC Labeled NB100-1965F
HRP Labeled NB100-1965H

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Blogs on Calnexin.

Calnexin - an ER chaperone that folds the cell's glycoproteins
Calnexin is an abundant 90kDa chaperone protein that resides in the membrane of the endoplasmic reticulum. Calnexin and the related calreticulin protein function together to ensure the proper folding of glycoproteins. By binding to partially folded...  Read full blog post.

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Gene Symbol CANX