Calnexin Antibody

Images

 
Western Blot: Calnexin Antibody [NB100-1965] - A dilution of 1/2000 used in TBS with 0.05% Tween-20. Bands visualized by ECL using goat anti-rabbit HRP at 1:2000.
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody [NB100-1965] - Antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
Immunohistochemistry: Calnexin Antibody [NB100-1965] - IHC analysis of CANX in mouse prostate using DAB with hematoxylin counterstain.
Western Blot: Calnexin Antibody [NB100-1965] - WB analysis of CANX in cell lysates as noted.
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody [NB100-1965] - Calnexin antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Simple Western: Calnexin Antibody [NB100-1965] - Simple Western lane view shows a specific band for Calnexin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Av, Bv, Ch, Dr, Gp, Rb, Sh, Xp, Ze, CaSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Calnexin Antibody Summary

Immunogen
A synthetic peptide made to an internal region of the canine Calnexin protein (within residues 25-100). [Swiss-Prot P24643]
Localization
Endoplasmic reticulum
Marker
Endoplasmic Reticulum Membrane Marker
Predicted Species
Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CANX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Simple Western 1:25
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:40
  • Immunohistochemistry-Paraffin 1:40
  • Immunoprecipitation 1:100
Application Notes
This Calnexin antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, Immunoprecipitation and Western Blot. In Western blot a band is observed approx. 97 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB100-1965 in the following applications:

Publications
Read Publications using
NB100-1965 in the following applications:

  • IB
    1 publication
  • 3 publications
  • IHC
    1 publication
  • WB
    13 publications

Reactivity Notes

Human, mouse, rat, Xenopus laevis, bovine, chicken, Drosophilia melanogaster, guinea pig, pig, quail, rabbit, zebrafish and sheep. Predicted to react with dog based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Calnexin Antibody

  • calnexin
  • CNX
  • IP90FLJ26570
  • Major histocompatibility complex class I antigen-binding protein p88
  • P90

Background

Calnexin is a chaperone protein that assists in protein folding and quality control. Calnexin retains unassembled or unfolded N-linked glycoproteins in the endoplasmic reticulum and ensures that only properly assembled and folded proteins proceed down the secretory pathway. Calnexin antibodies can be used as endoplasmic reticulum markers (ER markers) in immunostaining experiments

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calnexin Antibody (NB100-1965)(22)

We have publications tested in 5 confirmed species: Human, Mouse, Bovine, Porcine, Zebrafish.

We have publications tested in 4 applications: IB, ICC/IF, IHC, WB.


Filter By Application
IB
(1)
ICC/IF
(3)
IHC
(1)
WB
(13)
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Filter By Species
Human
(7)
Mouse
(7)
Bovine
(1)
Porcine
(1)
Zebrafish
(1)
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Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB100-1965 Applications Species
Xu Y, Zhu Y, Bawa F et al. HepatocyteSpecific Expression of Human Carboxylesterase 1 Attenuates Diet-Induced Steatohepatitis and Hyperlipidemia in Mice Hepatol Commun Feb 20 2020 [PMID: 32258948] (WB, Mouse) WB Mouse
Jo Y, Kim Ss, Garland K et Al. Enhanced ER-associated degradation of HMG CoA reductase causes embryonic lethality associated with Ubiad1 deficiency Elife Mar 2 2020 [PMID: 32118581] (IB, Mouse) IB Mouse
Musante L, Bontha Sv, La Salvia S et Al. Rigorous characterization of urinary extracellular vesicles (uEVs) in the low centrifugation pellet - a neglected source for uEVs Sci Rep Feb 28 2020 [PMID: 32111925] (WB, Human) WB Human
Xu Y, Xu Y, Zhu Y et al. Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis Mol. Ther. 2019 Sep 12 [PMID: 31604677]
Khalaj K, Miller JE, Lingegowda H et al. Extracellular vesicles from endometriosis patients are characterized by a unique miRNA-lncRNA signature JCI Insight Sep 19 2019 [PMID: 31534048] (WB, Human) WB Human
Jo Y, Hamilton JS, Hwang S et al. Schnyder corneal dystrophy-associated UBIAD1 inhibits ER-associated degradation of HMG CoA reductase in mice Elife Feb 20 2019 [PMID: 30785396] (WB, Mouse) WB Mouse
Tiedemann K, Sadvakassova G, Mikolajewicz N et al. Exosomal Release of L-Plastin by Breast Cancer Cells Facilitates Metastatic Bone Osteolysis Transl Oncol Dec 20 2018 [PMID: 30583289] (WB, Human) WB Human
Gao HN, Guo HY, Zhang H et al. Yak milk-derived exosomes promote proliferation of intestinal epithelial cells in hypoxic environment J. Dairy Sci. Dec 20 2018 [PMID: 30580945] (WB, Bovine) WB Bovine
Qi X, Schmiege P, Coutavas E et al. Two Patched molecules engage distinct sites on Hedgehog yielding a signaling-competent complex Science Aug 23 2018 [PMID: 30139912] (WB, Human) WB Human
Qi X, Schmiege P, Coutavas E et al. Structures of human Patched and its complex with native palmitoylated sonic hedgehog. Nature. Jul 11 2018 [PMID: 29995851] (WB, Human) WB Human
Show All 22 Publications.

Review for Calnexin Antibody (NB100-1965) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-1965:
Filter by Applications
WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot Calnexin NB100-1965
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reviewed by:
Elizabeth Soberg
WB Mouse 06/13/2018
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Summary

ApplicationWestern Blot
Sample TestedRAW 264.7 mouse monocyte/macrophage cell line
SpeciesMouse
LotC-3

Comments

Comments20 ug of whole cell lysate and exosomal lysate were loaded.
Primary was used @ 2 ug/mL.
Donkey anti-rabbit-HRP secondary was used @ 1:5000.
Entry level (ug sensitivity) ECL reagents were used.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calnexin Antibody (NB100-1965) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-1965AF350
Alexa Fluor 405 NB100-1965AF405
Alexa Fluor 488 NB100-1965AF488
Alexa Fluor 532 NB100-1965AF532
Alexa Fluor 594 NB100-1965AF594
Alexa Fluor 647 NB100-1965AF647
Alexa Fluor 700 NB100-1965AF700
Alexa Fluor 750 NB100-1965AF750
Biotin NB100-1965B
DyLight 350 NB100-1965UV
DyLight 405 NB100-1965V
DyLight 488 NB100-1965G
DyLight 550 NB100-1965R
DyLight 594 NB100-1965DL594
DyLight 650 NB100-1965C
DyLight 680 NB100-1965FR
DyLight 755 NB100-1965IR
FITC NB100-1965F
HRP NB100-1965H
Janelia Fluor 549 NB100-1965JF549
Janelia Fluor 646 NB100-1965JF646

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Bioinformatics Tool for Calnexin Antibody (NB100-1965)

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Blogs on Calnexin.

Calnexin - an ER chaperone that folds the cell's glycoproteins
Calnexin is an abundant 90kDa chaperone protein that resides in the membrane of the endoplasmic reticulum. Calnexin and the related calreticulin protein function together to ensure the proper folding of glycoproteins. By binding to partially folded...  Read full blog post.

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Elizabeth Soberg
06/13/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol CANX
Entrez
Uniprot
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