Calnexin Antibody - BSA Free

Images

 
Western Blot: Calnexin Antibody [NB100-1965] - WB analysis of CANX in cell lysates as noted.
Western Blot: Calnexin Antibody [NB100-1965] - Subcellular localization of TM6SF2.Immunoaffinity isolation of ER and Golgi complex from mouse liver. ER and Golgi fractions were prepared from mouse liver microsomes by ...read more
Immunohistochemistry: Calnexin Antibody [NB100-1965] - Calnexin immunostaining in the anterior (AI) and mid (MI) intestine of ctrl (control zebrafish), D.I.O. (diet-induced obesity zebrafish), D.I.O. flw 3,5-T2 (D.I.O. ...read more
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody [NB100-1965] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody [NB100-1965] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody [NB100-1965] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Flow Cytometry: Calnexin Antibody [NB100-1965] - An intracellular stain was performed on rat FR cells with Calnexin Antibody NB100-1965 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% ...read more
Western Blot: Calnexin Antibody [NB100-1965] - A dilution of 1/2000 used in TBS with 0.05% Tween-20. Bands visualized by ECL using goat anti-rabbit HRP at 1:2000.
Immunohistochemistry: Calnexin Antibody [NB100-1965] - IHC analysis of CANX in mouse prostate using DAB with hematoxylin counterstain.
Flow Cytometry: Calnexin Antibody [NB100-1965] - An intracellular stain was performed on Daudi cells with Calnexin Antibody NB100-1965 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% ...read more
Flow Cytometry: Calnexin Antibody [NB100-1965] - An intracellular stain was performed on NIH3T3 cells with Calnexin Antibody NB100-1965 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% ...read more
Simple Western: Calnexin Antibody [NB100-1965] - Simple Western lane view shows a specific band for Calnexin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Av, Bv, Ch, Dr, Gp, Rb, Sh, Xp, Ze, CaSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Calnexin Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal region of the canine Calnexin protein (within residues 25-100). [Swiss-Prot P24643]
Localization
Endoplasmic reticulum
Marker
Endoplasmic Reticulum Membrane Marker
Predicted Species
Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CANX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1-5 ug/ml
  • Immunohistochemistry 1:40
  • Immunohistochemistry-Paraffin 1:40
  • Immunoprecipitation 1:100
  • Simple Western 1:25
  • Western Blot 2 ug/ml
Application Notes
This Calnexin antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, Immunoprecipitation and Western Blot. In Western blot a band is observed approx. 97 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4
using
NB100-1965 in the following applications:

Publications
Read Publications using
NB100-1965 in the following applications:

  • IB
    1 publication
  • 3 publications
  • IHC
    2 publications
  • WB
    17 publications

Reactivity Notes

Human, mouse, rat, Xenopus laevis, bovine, chicken, Drosophilia melanogaster, guinea pig, pig, quail, rabbit, zebrafish and sheep. Predicted to react with dog based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Calnexin Antibody - BSA Free

  • calnexin
  • CNX
  • IP90FLJ26570
  • Major histocompatibility complex class I antigen-binding protein p88
  • P90

Background

Calnexin is a chaperone protein that assists in protein folding and quality control. Calnexin retains unassembled or unfolded N-linked glycoproteins in the endoplasmic reticulum and ensures that only properly assembled and folded proteins proceed down the secretory pathway. Calnexin antibodies can be used as endoplasmic reticulum markers (ER markers) in immunostaining experiments

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calnexin Antibody (NB100-1965)(30)

We have publications tested in 5 confirmed species: Human, Mouse, Bovine, Porcine, Zebrafish.

We have publications tested in 4 applications: IB, ICC/IF, IHC, WB.


Filter By Application
IB
(1)
ICC/IF
(3)
IHC
(2)
WB
(17)
All Applications
Filter By Species
Human
(9)
Mouse
(9)
Bovine
(1)
Porcine
(1)
Zebrafish
(2)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using NB100-1965 Applications Species
Xu Y, Hu S, Jadhav K et al. Hepatocytic Activating Transcription Factor 3 Protects Against Steatohepatitis Via Hepatocyte Nuclear Factor 4 alpha Diabetes Sep 2 2021 [PMID: 34475098]
Xu Y, Zhu Y, Hu S et al. Hepatocyte miR-34a is a key regulator in the development and progression of non-alcoholic fatty liver disease Molecular metabolism Apr 27 2021 [PMID: 33930596] (WB, Mouse) WB Mouse
Millan C, Prause L, Vallmajo-Martin Q et al. Extracellular Vesicles from 3D Engineered Microtissues Harbor Disease-Related Cargo Absent in EVs from 2D Cultures Advanced healthcare materials Apr 22 2021 [PMID: 33890421]
Xu Y, Li Y, Jadhav K, et al. Hepatocyte ATF3 protects against atherosclerosis by regulating HDL and bile acid metabolism Nature metabolism Jan 1 2021 [PMID: 33462514]
Qi X, Friedberg L, De Bose-Boyd R et al. Sterols in an intramolecular channel of Smoothened mediate Hedgehog signaling Nat. Chem. Biol. Sep 14 2020 [PMID: 32929279] (WB, Human) WB Human
Imperatore R, Tunisi L, Mavaro I et al. Immunohistochemical Analysis of Intestinal and Central Nervous System Morphology in an Obese Animal Model (Danio rerio) Treated with 3,5-T2: A Possible Farm Management Practice? Animals (Basel) Jul 3 2020 [PMID: 32635261] (IHC, Zebrafish) IHC Zebrafish
Xu Y, Zhu Y, Bawa F et al. HepatocyteSpecific Expression of Human Carboxylesterase 1 Attenuates Diet-Induced Steatohepatitis and Hyperlipidemia in Mice Hepatol Commun Feb 20 2020 [PMID: 32258948] (WB, Mouse) WB Mouse
Jo Y, Kim Ss, Garland K et Al. Enhanced ER-associated degradation of HMG CoA reductase causes embryonic lethality associated with Ubiad1 deficiency Elife Mar 2 2020 [PMID: 32118581] (IB, Mouse) IB Mouse
Musante L, Bontha Sv, La Salvia S et Al. Rigorous characterization of urinary extracellular vesicles (uEVs) in the low centrifugation pellet - a neglected source for uEVs Sci Rep Feb 28 2020 [PMID: 32111925] (WB, Human)

Details:
Antibody was used in Western Blot analysis to verify that subcellular contaminants were not interfering with detecting target bio-markers.
WB Human
Musante L, Bontha Sv, La Salvia S et Al. Rigorous characterization of urinary extracellular vesicles (uEVs) in the low centrifugation pellet - a neglected source for uEVs Sci Rep Feb 28 2020 [PMID: 32111925] (WB, Human)

Details:
Antibody was used in Western Blot analysis to verify that subcellular contaminants were not interfering with detecting target bio-markers.
WB Human
Show All 30 Publications.

Reviews for Calnexin Antibody (NB100-1965) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-1965:
Filter by Applications
Immunoprecipitation
(1)
Western Blot
(2)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunoprecipitation Calnexin NB100-1965
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2
reviewed by:
Verified Customer
Immunoprecipitation Human 03/14/2022
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Summary

ApplicationImmunoprecipitation
Sample TestedB cell lymphoma
SpeciesHuman
LotE

Comments

Comments***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
Western Blot Calnexin NB100-1965
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5
reviewed by:
Verified Customer
Western Blot Human 02/16/2022
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Summary

ApplicationWestern Blot
Sample Tested293t HEK and U2OS cells
SpeciesHuman
LotE

Comments

CommentsLane 1: WCL of 5 x 10^6 HEK cells
Lane 2: Membrane enrichment of 5 x 10^6 HEK cells
Size is around 75 kDA. One clear and specific band.
Western Blot Calnexin NB100-1965
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5
reviewed by:
Verified Customer
Western Blot Mouse 06/13/2018
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Summary

ApplicationWestern Blot
Sample TestedRAW 264.7 mouse monocyte/macrophage cell line
SpeciesMouse
LotC-3

Comments

Comments20 ug of whole cell lysate and exosomal lysate were loaded.
Primary was used @ 2 ug/mL.
Donkey anti-rabbit-HRP secondary was used @ 1:5000.
Entry level (ug sensitivity) ECL reagents were used.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calnexin Antibody (NB100-1965) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Calnexin Antibody (NB100-1965)

Discover related pathways, diseases and genes to Calnexin Antibody (NB100-1965). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Calnexin.

Calnexin - an ER chaperone that folds the cell's glycoproteins
Calnexin is an abundant 90kDa chaperone protein that resides in the membrane of the endoplasmic reticulum. Calnexin and the related calreticulin protein function together to ensure the proper folding of glycoproteins. By binding to partially folded...  Read full blog post.

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Recent Reviews

4
5
2
4
0
3
0
2
1
1
0

Verified Customer
03/14/2022
Application: Immunoprecipitation
Species: Human

Verified Customer
02/16/2022
Application: Western Blot
Species: Human

Verified Customer
06/13/2018
Application: Western Blot
Species: Mouse

Bioinformatics

Gene Symbol CANX
Entrez
Uniprot