Protein Disulfide Isomerase/P4HB Antibody (RL90)

Images

 
Western Blot: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Analysis of Protein Disulfide Isomerase/P4HB was performed by loading 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates.
Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Staining of Phalloidin (purple) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with ...read more
Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Both normal and cancer biopsies of deparaffinized Human colon tissue tissues.
Flow Cytometry: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Analysis of Hela cells compared to an isotype control (blue).
Western Blot: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Analysis of PDIA2 expression in 1) human brain and 2) rat liver tissues extracts, 3) HeLa and 4) Hek 293 whole cell lysates.
Western Blot: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Cell surface Protein Disulfide Isomerase/P4HB associated with GC-AC. Proteins were pulled down with anti GC-A antibody from HUVEC or HMC ...read more
Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Staining in p19 Cells.
Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and ...read more
Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and ...read more
Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and ...read more
Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Staining in A549 Cells.
Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Staining in HMVEC Cells.
Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Staining in NS-1 Cells.
Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Cancer biopsies of deparaffinized Human lung adenocarcinoma tissues.
Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Flow Cytometry: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Analysis of NIH/3T3 cells compared to an isotype control (blue).
Flow Cytometry: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Analysis of K562 cells compared to an isotype control (blue).
Genetic Strategies: Knockdown Validated: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Down regulated PDIA2 attenuates CNP-mediated cGMP activation in HMCs. Western blotting with RL90 antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ha, Pm, Pm, RbSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IP, B/N, KD
Clone
RL90
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

Protein Disulfide Isomerase/P4HB Antibody (RL90) Summary

Immunogen
Purified rat Protein Disulfide Isomerase/P4HB protein.
Marker
Endoplasmic Reticulum Marker
Specificity
NB300-517 detects Protein Disulfide Isomerase/P4HB from human, rat, porcine and mouse tissues as well as hamster cells.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
P4HB
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunomicroscopy 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Knockdown Validated
  • Western Blot 1:1000
Application Notes
IHC usage was reported in scientific literature (see Tanaka S, et al). ELISA assay reported in literature (see Kaetzel CS, et al). Flow Cytometry usage was reported in scientific literature (see Ryser HJ, et al). Blocking/Neutralization usage was reported in the literature (PMID:12791669 and PMID: 22013897)
Control
HeLa Whole Cell Lysate
Reviewed Applications
Read 3 Reviews rated 5
using
NB300-517 in the following applications:

Publications
Read Publications using
NB300-517 in the following applications:

  • B/N
    2 publications
  • 1 publication
  • 4 publications
  • IHC
    1 publication
  • WB
    4 publications

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 16150729). Bovine (PMID: 14592831). Primate (PMID: 20667829). Monkey reactivity reported in scientific literature (PMID: 27487388). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Protein Disulfide Isomerase/P4HB Antibody (RL90)

  • Cellular thyroid hormone-binding protein
  • collagen prolyl 4-hydroxylase beta
  • DSI
  • ERBA2L
  • GIT
  • glutathione-insulin transhydrogenase
  • P4HB
  • P4Hbeta
  • p55
  • PDI
  • PDIA1
  • PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1)
  • PDIEC 5.3.4.1
  • PHDB
  • PO4DB
  • PO4HB
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide
  • PROHB
  • PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase; thyroid hormone binding protein p55)
  • Prolyl 4-hydroxylase subunit beta
  • prolyl 4-hydroxylase, beta polypeptide
  • protein disulfide isomerase family A, member 1
  • protein disulfide isomerase/oxidoreductase
  • protein disulfide isomerase-associated 1
  • protein disulfide-isomerase
  • protocollagen hydroxylase
  • Thbp
  • thyroid hormone-binding protein p55

Background

P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Protein Disulfide Isomerase/P4HB Antibody (NB300-517)(22)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Monkey, Primate.

We have publications tested in 5 applications: B/N, Flow, ICC/IF, IHC, WB.


Filter By Application
B/N
(2)
Flow
(1)
ICC/IF
(4)
IHC
(1)
WB
(4)
All Applications
Filter By Species
Human
(6)
Mouse
(2)
Rat
(1)
Monkey
(1)
Primate
(1)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB300-517 Applications Species
Beckmann L, Rolling CC, VoigtlAnder M Et al. Bacitracin and Rutin Regulate Tissue Factor Production in Inflammatory Monocytes and Acute Myeloid Leukemia Blasts Cancers Aug 4 2021 [PMID: 34439096]
Paardekooper LM, van Vroonhoven E, Ter Beest M, van den Bogaart G Radical Stress Is More Cytotoxic in the Nucleus than in Other Organelles Int J Mol Sci Aug 25 2019 [PMID: 31450682] (WB, Human) WB Human
Subramanian A, Capalbo A, Iyengar NR et al. Auto-regulation of Secretory Flux by Sensing and Responding to the Folded Cargo Protein Load in the Endoplasmic Reticulum Cell Mar 7 2019 [PMID: 30849374] (ICC/IF, Human) ICC/IF Human
Liang D, Wu Y, Zhou L et al. LRP5 controls cardiac QT interval by modulating the metabolic homeostasis of L-type calcium channel. Int J Cardiol. [PMID: 30309679] (ICC/IF, Mouse) ICC/IF Mouse
Zorin VL, Pulin AA, Eremin II et al. Myogenic potential of human alveolar mucosa derived cells Cell Cycle 2017 Mar 19 [PMID: 28118065] (ICC/IF, Flow, Human) ICC/IF, Flow Human
Dosoki H, Stegemann A, Taha M et al. Targeting of NADPH oxidase in vitro and in vivo suppresses fibroblast activation and experimental skin fibrosis. Exp. Dermatol. 2016 Aug 30 [PMID: 27576129]
Walsh MT, Di Leo E, Okur I et al. Structure-function analyses of microsomal triglyceride transfer protein missense mutations in abetalipoproteinemia and hypobetalipoproteinemia subjects. Biochim. Biophys. Acta. Jul 31 2016 [PMID: 27487388] (WB, Monkey) WB Monkey
Pan S, Chen Hh, Correia C et al. Cell surface protein disulfide isomerase regulates natriuretic Peptide generation of cyclic guanosine monophosphate. PLoS OnE. 2014 Nov 25 [PMID: 25419565] (ICC/IF, Human) ICC/IF Human
Langer F, Spath B, Fischer C et al. Rapid activation of monocyte tissue factor by antithymocyte globulin is dependent on complement and protein disulfide isomerase. Blood 2013 Jan 11 [PMID: 23315166]
Hicks DA, Makova NZ, Nalivaeva NN et al. Characterisation of acetylcholinesterase release from neuronal cells. Chem Biol Interact. 2013 Mar 25 [PMID: 23047022] (B/N, Mouse) B/N Mouse
Show All 22 Publications.

Reviews for Protein Disulfide Isomerase/P4HB Antibody (NB300-517) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Other.

Reviews using NB300-517:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(2)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Protein Disulfide Isomerase/P4HB NB300-517
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5
reviewed by:
Jaehyung (Gus) Cho
WB Human 06/20/2011
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Summary

ApplicationWestern Blot
Sample TestedHL60 cells transduced with a lentiviral control or PDI shRNA vector., Sample Amount: Lysates of 50,000 HL60 cells
SpeciesHuman
LotLB138171 and MA151519
Comments1. A band of protein disulfide isomerase is appeared at 65-kD.

I am very satisfied with the products supplied by NOVUS Biologicals, especially the high quality of many antibodies. In particular, I have been using monoclonal antibodies against protein disulfide isomerase since 2009. These antibodies work extremely well for detection of human protein disulfide isomerase and block its function well. The price of NOVUS Biologicals antibodies is very reasonable in comparison to that of other companies. I strongly recommend NOVUS Biologicals products to my colleagues.

Blocking

Blocking DetailsBlocking Buffer: 5% BSA in TBS, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:1000, Incubation Dilution Buffer:TBS containing 0.1% BSA and 0.1% Tween-20, Incubation Time:1hr, Temp:RT

Secondary Antibody

Secondary DescriptionSecondary Ab: HRP-conjugated anti-mouse IgG, Secondary Ab Dilution Ratio: 1:10,000
Secondary Manufacturer Cat#Jackson Immunoresearch, 715-035-150

Details

Detection NotesDetection Method: Chemiluminescence, Exposure Time: 10 sec- 1min, Specific Bands: PDI at 65 kDa, Non-Specific Bands: None

Comments

Comments1. A band of protein disulfide isomerase is appeared at 65-kD.

I am very satisfied with the products supplied by NOVUS Biologicals, especially the high quality of many antibodies. In particular, I have been using monoclonal antibodies against protein disulfide isomerase since 2009. These antibodies work extremely well for detection of human protein disulfide isomerase and block its function well. The price of NOVUS Biologicals antibodies is very reasonable in comparison to that of other companies. I strongly recommend NOVUS Biologicals products to my colleagues.
  5
reviewed by:
Verified Customer
WB Human 06/23/2010
View

Summary

ApplicationWestern Blot
Sample TestedCell lysate
SpeciesHuman
CommentsThis antibody was pure and worked fine.

Comments

CommentsThis antibody was pure and worked fine.
  5
reviewed by:
Verified Customer
WB Other 03/11/2010
View

Summary

ApplicationWestern Blot
Sample Testedplasma, Sample Amount: 20ul
SpeciesOther
LotKE132987

Blocking

Blocking DetailsBlocking Buffer: 5% milk, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/1000, Incubation Dilution Buffer: 1% milk, Incubation Time: overnight, Incubation Temp: RT

Secondary Antibody

Secondary DescriptionSecondary Ab: donkey anti mouse HRP, Secondary Ab Dilution Ratio: 1/10000

Details

Detection NotesDetection Method: ECL Plus, Exposure Time: 2 seconds, Specific Bands: 60 kDa

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Protein Disulfide Isomerase/P4HB Antibody (NB300-517). (Showing 1 - 1 of 1 FAQs).

  1. We have recently purchased mouse monoclonal anti-PDI antibody RL90 (cat# NB300-517). From the information available, it appears that this was generated with purified rat PDI serving as immunogen. We would like to know whether there is any information available on the epitope in the PDI molecule recognized by the RL90 antibody? I could not find this information in the description of the antibody.
    • Yes, you are correct: purified rat PDI protein was used as the immunogen for NB300-517, PDI Antibody (RL90). We do not normally epitope map our antibodies, so I think it is unlikely that we know where on the PDI protein this antibody binds. The lab have now confirmed to me that no epitope mapping been carried out for NB300-517, and hence we do not have any additional detail on the binding region of this antibody.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Jaehyung (Gus) Cho
06/20/2011
Application: WB
Species: Human

 
Verified Customer
06/23/2010
Application: WB
Species: Human

 
Verified Customer
03/11/2010
Application: WB
Species: Other

Bioinformatics

Gene Symbol P4HB