ERp72 Antibody (OTI2B9)

Images

 
Western Blot: ERp72 Antibody (2B9) [NBP2-01652] Analysis of extracts (35ug) from 9 different cell lines by using anti-ERp72 monoclonal antibody.
Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Human tonsil using anti-ERp72 mouse monoclonal antibody.
Western Blot: ERp72 Antibody (2B9) [NBP2-01652] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERp72 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ERp72 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ERp72 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ERp72 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Human liver tissue using anti-ERp72 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Human lymphoma tissue using anti-ERp72 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Human pancreas tissue using anti-ERp72 mouse monoclonal antibody.
Western Blot: ERp72 Antibody (2B9) [NBP2-01652] - Western Blot of lysate of PC-3 cells. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, IHC
Clone
OTI2B9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.84 mg/ml

Order Details

ERp72 Antibody (OTI2B9) Summary

Immunogen
Full length human recombinant protei of human PDIA4(NP_004902) produced in HEK293T cell.
Marker
Endoplasmic Reticulum Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PDIA4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
72.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-01652 in the following applications:

Publications
Read Publication using
NBP2-01652 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.84 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ERp72 Antibody (OTI2B9)

  • Endoplasmic reticulum resident protein 70
  • Endoplasmic reticulum resident protein 72
  • ER protein 70
  • ERp70
  • ERP70ER protein 72
  • ERp72
  • ERp-72
  • ERP72EC 5.3.4.1
  • intestinal-related)
  • protein disulfide isomerase family A, member 4
  • protein disulfide isomerase related protein (calcium-binding protein
  • protein disulfide isomerase-associated 4
  • protein disulfide-isomerase A4

Background

ERp72 is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. Sequence analysis of ERp72 shows that it shares sequence identity with PDI (protein disulphide isomerase) at three discrete regions, as well as containing the ER retention signal KEEL at its C-terminus. ERp72 is shown to have three copies of sequence believed to be CGHC-containing active sites, which are also found in PDI. These CGHC sites are critical sites that are involved in the protein disulphide isomerization process. These results suggest that ERp72 is a newly described member of the family of CGHC-containing proteins that functions as a molecular chaperone. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERp72 Antibody (NBP2-01652)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Review for ERp72 Antibody (NBP2-01652) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-01652:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot ERp72 NBP2-01652
Enlarge
5
reviewed by:
Armen Petrosyan
WB Human 06/27/2023
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Summary

ApplicationWestern Blot
Sample TestedPC-3 human prostate cancer cell line
SpeciesHuman
LotA01

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Recent Reviews

5
5
1
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1
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Armen Petrosyan
06/27/2023
Application: WB
Species: Human

Bioinformatics

Gene Symbol PDIA4
Entrez