Notch-1 Antibody

Images

 
Western Blot: Notch-1 Antibody [NBP1-78292] - Mouse total protein lysates (50 ug) were separated on 8% SDS-PAGE. Notch-1 (NBP1-78292SS) antibody was used at 1:500 dilution. Actin (1:10,000) was used as normalizer. WB ...read more
Immunohistochemistry: Notch-1 Antibody [NBP1-78292] - Staining of Notch1 in human kidney carcinoma using DAB with hematoxylin counterstain.
Western Blot: Notch-1 Antibody [NBP1-78292] - Analysis of Cleaved Notch1 in Jurkat cell lysate.
Simple Western: Notch-1 Antibody [NBP1-78292] - Lane view shows a specific band for Notch-1 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.18 mg/ml

Notch-1 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human NOTCH1 protein (between residues 2300-2350) [UniProt# P46531]
Localization
Cell membrane; Single-pass type I membrane protein. Notch 1 intracellular domain: Following proteolytical processing is translocated to the nucleus.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOTCH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This Notch1 antibody is useful for Western Blot and Immunohistochemistry-Paraffin. In Western Blot, a band is seen ~120 kDa representing the cleaved form of Notch1 peptide. In IHC-P, staining of nucleus and cell membrane was observed in human kidney carcinoma tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
Jurkat Whole Cell Lysate
Notch-1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP1-78292 in the following applications:

Publications
Read Publications using
NBP1-78292 in the following applications:

  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.18 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Notch-1 Antibody

  • EC 2.1.2.11
  • EC 3.4.21.68
  • hN1
  • Notch (Drosophila) homolog 1 (translocation-associated)
  • notch 1Notch homolog 1, translocation-associated (Drosophila)
  • Notch homolog 1, translocation-associated
  • Notch1
  • Notch-1
  • TAN1
  • TAN1neurogenic locus notch homolog protein 1
  • Translocation-associated notch protein TAN-1

Background

Notch1 is a single-pass transmembrane protein that serves as the receptor for the Delta1, Jagged1 and Jagged2 ligands. Activation of Notch1 causes the cytoplasmic domain to be cleaved, releasing the notch intracellular domain (NICD) which forms a complex with RBPJ/RBPSUH and subsequently influences transcription of its target genes. Because the majority of Notch1 ligands are transmembrane proteins, Notch1 is primarily triggered by cell-to-cell contact and employs lateral inhibition mechanisms to influence local populations of cells. The Notch1 pathway plays a pivotal role in development, including cell-fate determination, proliferation, and cell contact-dependent signaling. Aberrant activation of the Notch1 pathway has been associated with numerous diseases such as bicuspid aortic valve and cancer-- in particular certain leukemias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-78292
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

Publications for Notch-1 Antibody (NBP1-78292)(3)

Review for Notch-1 Antibody (NBP1-78292) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-78292:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot Notch-1 NBP1-78292
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reviewed by:
Manjinder Cheema
WB Mouse 02/18/2020
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Summary

ApplicationWestern Blot
Sample Tested Mouse brain,mouse liver,Mouse Spleen,Mouse kidney
SpeciesMouse
LotA-2

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Notch-1 Antibody (NBP1-78292). (Showing 1 - of FAQs).

    Control Lysate(s)

    Secondary Antibodies

     

    Isotype Controls

    Other Available Formats

    Alexa Fluor 350 NBP1-78292AF350
    Alexa Fluor 405 NBP1-78292AF405
    Alexa Fluor 488 NBP1-78292AF488
    Alexa Fluor 532 NBP1-78292AF532
    Alexa Fluor 594 NBP1-78292AF594
    Alexa Fluor 647 NBP1-78292AF647
    Alexa Fluor 700 NBP1-78292AF700
    Alexa Fluor 750 NBP1-78292AF750
    Biotin NBP1-78292B
    DyLight 350 NBP1-78292UV
    DyLight 405 NBP1-78292V
    DyLight 488 NBP1-78292G
    DyLight 550 NBP1-78292R
    DyLight 594 NBP1-78292DL594
    DyLight 650 NBP1-78292C
    DyLight 680 NBP1-78292FR
    DyLight 755 NBP1-78292IR
    FITC NBP1-78292F
    HRP NBP1-78292H
    Janelia Fluor 549 NBP1-78292JF549
    Janelia Fluor 646 NBP1-78292JF646

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    Bioinformatics Tool for Notch-1 Antibody (NBP1-78292)

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    Research Areas for Notch-1 Antibody (NBP1-78292)

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    Blogs on Notch-1.

    Notch1 - A multifunctional transmembrane receptor
    Notch1 is a member of the Notch family of Type 1 single-pass transmembrane proteins that share an extracellular domain of multiple epidermal growth factor-like (EGF) repeats. Notch family members play key roles in a variety of developmental process...  Read full blog post.

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    Recent Reviews

    5
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    Manjinder Cheema
    02/18/2020
    Application: WB
    Species: Mouse

    Bioinformatics

    Gene Symbol NOTCH1
    Entrez
    Uniprot