HES-1 Antibody (OTI4H1)

Western Blot: HES-1 Antibody (OTI4H1) [NBP1-47791] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1 (1:500).

Immunocytochemistry/Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791] - HeLa cells using anti-HES1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded liver using anti-Hes1 mouse monoclonal antibody.

Immunocytochemistry/Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hes1.

Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded breast using anti-Hes1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded Carcinoma of liver using anti-Hes1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded endometrium using anti-Hes1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded kidney using anti-Hes1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ChIP, ICC/IF, IHC, IHC-P
• Clone: OTI4H1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

HES-1 Antibody (OTI4H1) Summary

• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 1-280 of human HES1
• Specificity: This antibody is specific for Homo sapiens hairy and enhancer of split 1, (Drosophila) (HES1).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HES1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
• Application Notes: Chip reactivity reported in (PMID: 29048612).
• Theoretical MW: 29.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HES-1 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for HES-1 Antibody (OTI4H1)

• BHLHB39
• bHLHb39hairy homolog (Drosophila)
• Class B basic helix-loop-helix protein 39
• FLJ20408
• Hairy and enhancer of split 1
• hairy and enhancer of split 1, (Drosophila)
• Hairy homolog
• Hairy-like protein
• HES1
• HES-1
• hHL
• HL
• HRY
• HRYHHL
• transcription factor HES-1

Background

This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HES-1 Antibody (NBP1-47791)(6)

Publications using NBP1-47791

• Kim TH, Jeong JY, Park JY, Kim SW et al. miR-150 enhances apoptotic and anti-tumor effects of paclitaxel in paclitaxel-resistant ovarian cancer cells by targeting Notch3 Oncotarget 2017 Sep 22 [PMID: 29069826] (WB, Human)
• Liao B, Zhou H, Liang H, Li C. Regulation of ERK and AKT pathways by hepatitis B virus X protein via the Notch1 pathway in hepatocellular carcinoma. Int. J. Oncol. 2017 Nov 01 [PMID: 29048612] (ChIP, Human)
• Jeong JY, Kang H, Kim TH et al. MicroRNA-136 inhibits cancer stem cell activity and enhances the anti-tumor effect of paclitaxel against chemoresistant ovarian cancer cells by targeting Notch3. Cancer Lett. Nov 22 2016 [PMID: 27887917] (WB, Human)
• Chiou HY,Liu SY,Lin CH, Lee EH. Hes-1 SUMOylation by protein inhibitor of activated STAT1 enhances the suppressing effect of Hes-1 on GADD45a expression to increase cell survival J. Biomed. Sci. 2014 Jun 26 [PMID: 24894488] (ChIP, Human)
  
  Details:
  Hes1 antibody used for Chromatin Immunoprecipitation /ChIP assay in transfected HEK293T cells - nuclear chromatin incubated Hes-1 antibody 4.8 ug or mouse IgG or Flag-M2, 4C ON, immunoprecipitates collected on magnesium beads (1-2 h 4C), immunoprecipitates washed and de-crosslinked, chromatin processed for quantitative PCR analysis (Figure 6B).
• Maraver A, Fernandez-Marcos PJ, Herranz D et al. Therapeutic Effect of Secretase Inhibition in Kras(G12V)-Driven Non-Small Cell Lung Carcinoma by Derepression of DUSP1 and Inhibition of ERK Cancer Cell 2012 Aug 14 [PMID: 22897852] (ChIP, Human)
• Wang CY, Wei Q, Han I et al. Hedgehog and Notch signaling regulate self-renewal of undifferentiated pleomorphic sarcomas. Cancer Res 2012 Feb 15 [PMID: 22232736]

FAQs for HES-1 Antibody (NBP1-47791). (Showing 1 - 1 of 1 FAQs).

1. Can you elaborate in detail regarding the specificity mentioned for NBP1-47791?
   • This antibody has been created to detect the human HES1 protein (UniProt Q14469). The immunogen information given states that the immunogen corresponds to amino acids 1-280 of human HES1, which according to the UniProt entry I've just linked to is the whole protein. I think the mention of Drosophila in the specificity information is referring to the derivation of the name of this protein. Wikipedia says, 'Transcription factor HES1 (hairy and enhancer of split-1) is a protein that is encoded by the Hes1 gene, and is the mammalian homolog of the hairy gene in Drosophila'.

Bioinformatics

• Gene Symbol: HES1
• Entrez:
  • 3280(Human)
• Uniprot:
  • Q14469()