HES-1 Antibody (OTI4H1)


Western Blot: HES-1 Antibody (OTI4H1) [NBP1-47791] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791] - HeLa cells using anti-HES1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded liver using anti-Hes1 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hes1.
Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded breast using anti-Hes1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded Carcinoma of liver using anti-Hes1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded endometrium using anti-Hes1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded kidney using anti-Hes1 mouse monoclonal antibody.

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P
1 mg/ml

HES-1 Antibody (OTI4H1) Summary

Recombinant protein expressed in E.coli corresponding to amino acids 1-280 of human HES1
This antibody is specific for Homo sapiens hairy and enhancer of split 1, (Drosophila) (HES1).
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:1000
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
Application Notes
Chip reactivity reported in (PMID: 29048612).
Theoretical MW
29.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
HES-1 Knockout HeLa Cell Lysate
Read Publications using
NBP1-47791 in the following applications:

  • 3 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
0.02% Sodium Azide
1 mg/ml
Affinity purified

Alternate Names for HES-1 Antibody (OTI4H1)

  • BHLHB39
  • bHLHb39hairy homolog (Drosophila)
  • Class B basic helix-loop-helix protein 39
  • FLJ20408
  • Hairy and enhancer of split 1
  • hairy and enhancer of split 1, (Drosophila)
  • Hairy homolog
  • Hairy-like protein
  • HES1
  • HES-1
  • hHL
  • HL
  • HRY
  • transcription factor HES-1


This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HES-1 Antibody (NBP1-47791)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ChIP, WB.

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Showing Publications 1 - 6 of 6.
Publications using NBP1-47791 Applications Species
Kim TH, Jeong JY, Park JY, Kim SW et al. miR-150 enhances apoptotic and anti-tumor effects of paclitaxel in paclitaxel-resistant ovarian cancer cells by targeting Notch3 Oncotarget 2017 Sep 22 [PMID: 29069826] (WB, Human) WB Human
Liao B, Zhou H, Liang H, Li C. Regulation of ERK and AKT pathways by hepatitis B virus X protein via the Notch1 pathway in hepatocellular carcinoma. Int. J. Oncol. 2017 Nov 01 [PMID: 29048612] (ChIP, Human) ChIP Human
Jeong JY, Kang H, Kim TH et al. MicroRNA-136 inhibits cancer stem cell activity and enhances the anti-tumor effect of paclitaxel against chemoresistant ovarian cancer cells by targeting Notch3. Cancer Lett. Nov 22 2016 [PMID: 27887917] (WB, Human) WB Human
Chiou HY,Liu SY,Lin CH, Lee EH. Hes-1 SUMOylation by protein inhibitor of activated STAT1 enhances the suppressing effect of Hes-1 on GADD45a expression to increase cell survival J. Biomed. Sci. 2014 Jun 26 [PMID: 24894488] (ChIP, Human)

Hes1 antibody used for Chromatin Immunoprecipitation /ChIP assay in transfected HEK293T cells - nuclear chromatin incubated Hes-1 antibody 4.8 ug or mouse IgG or Flag-M2, 4C ON, immunoprecipitates collected on magnesium beads (1-2 h 4C), immunoprecipitates washed and de-crosslinked, chromatin processed for quantitative PCR analysis (Figure 6B).
ChIP Human
Maraver A, Fernandez-Marcos PJ, Herranz D et al. Therapeutic Effect of Secretase Inhibition in Kras(G12V)-Driven Non-Small Cell Lung Carcinoma by Derepression of DUSP1 and Inhibition of ERK Cancer Cell 2012 Aug 14 [PMID: 22897852] (ChIP, Human) ChIP Human
Wang CY, Wei Q, Han I et al. Hedgehog and Notch signaling regulate self-renewal of undifferentiated pleomorphic sarcomas. Cancer Res 2012 Feb 15 [PMID: 22232736]

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for HES-1 Antibody (NBP1-47791). (Showing 1 - 1 of 1 FAQs).

  1. Can you elaborate in detail regarding the specificity mentioned for NBP1-47791?
    • This antibody has been created to detect the human HES1 protein (UniProt Q14469). The immunogen information given states that the immunogen corresponds to amino acids 1-280 of human HES1, which according to the UniProt entry I've just linked to is the whole protein. I think the mention of Drosophila in the specificity information is referring to the derivation of the name of this protein. Wikipedia says, 'Transcription factor HES1 (hairy and enhancer of split-1) is a protein that is encoded by the Hes1 gene, and is the mammalian homolog of the hairy gene in Drosophila'.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70937AF350
Alexa Fluor 405 NBP2-70937AF405
Alexa Fluor 488 NBP2-70937AF488
Alexa Fluor 532 NBP2-70937AF532
Alexa Fluor 594 NBP2-70937AF594
Alexa Fluor 647 NBP2-70937AF647
Alexa Fluor 700 NBP2-70937AF700
Alexa Fluor 750 NBP2-70937AF750
Biotin NBP2-70937B
DyLight 350 NBP2-70937UV
DyLight 405 NBP2-70937V
DyLight 488 NBP2-70937G
DyLight 550 NBP2-70937R
DyLight 594 NBP2-70937DL594
DyLight 650 NBP2-70937C
DyLight 680 NBP2-70937FR
DyLight 755 NBP2-70937IR
FITC NBP2-70937F
HRP NBP2-70937H
Janelia Fluor 549 NBP2-70937JF549
Janelia Fluor 646 NBP2-70937JF646

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Gene Symbol HES1