HES-1 Antibody (OTI4H1)

Western Blot: HES-1 Antibody (OTI4H1) [NBP1-47791] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1 (1:500).

Immunocytochemistry/Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791] - HeLa cells using anti-HES1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded liver using anti-Hes1 mouse monoclonal antibody.

Immunocytochemistry/Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hes1.

Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded breast using anti-Hes1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded Carcinoma of liver using anti-Hes1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded endometrium using anti-Hes1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded kidney using anti-Hes1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ChIP, ICC/IF, IHC, IHC-P
• Clone: OTI4H1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

HES-1 Antibody (OTI4H1) Summary

• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 1-280 of human HES1
• Specificity: This antibody is specific for Homo sapiens hairy and enhancer of split 1, (Drosophila) (HES1).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HES1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000
• Application Notes: Chip reactivity reported in (PMID: 29048612).
• Theoretical MW: 29.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HES-1 Antibody (OTI4H1)

• BHLHB39
• bHLHb39hairy homolog (Drosophila)
• Class B basic helix-loop-helix protein 39
• FLJ20408
• Hairy and enhancer of split 1
• hairy and enhancer of split 1, (Drosophila)
• Hairy homolog
• Hairy-like protein
• HES1
• HES-1
• hHL
• HL
• HRY
• HRYHHL
• transcription factor HES-1

Background

This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HES-1 Antibody (NBP1-47791)(7)

Publications using NBP1-47791

• Abe T, Uosaki H, Shibata H, et al. Fetal sheep support the development of hematopoietic cells in vivo from human induced pluripotent stem cells Experimental hematology Jan 1 2021 [PMID: 33395577] (IHC, Human)
• Kim TH, Jeong JY, Park JY, Kim SW et al. miR-150 enhances apoptotic and anti-tumor effects of paclitaxel in paclitaxel-resistant ovarian cancer cells by targeting Notch3 Oncotarget 2017 Sep 22 [PMID: 29069826] (WB, Human)
• Liao B, Zhou H, Liang H, Li C. Regulation of ERK and AKT pathways by hepatitis B virus X protein via the Notch1 pathway in hepatocellular carcinoma. Int. J. Oncol. 2017 Nov 01 [PMID: 29048612] (ChIP, Human)
• Jeong JY, Kang H, Kim TH et al. MicroRNA-136 inhibits cancer stem cell activity and enhances the anti-tumor effect of paclitaxel against chemoresistant ovarian cancer cells by targeting Notch3. Cancer Lett. Nov 22 2016 [PMID: 27887917] (WB, Human)
• Chiou HY,Liu SY,Lin CH, Lee EH. Hes-1 SUMOylation by protein inhibitor of activated STAT1 enhances the suppressing effect of Hes-1 on GADD45a expression to increase cell survival J. Biomed. Sci. 2014 Jun 26 [PMID: 24894488] (ChIP, Human)
  
  Details:
  Hes1 antibody used for Chromatin Immunoprecipitation /ChIP assay in transfected HEK293T cells - nuclear chromatin incubated Hes-1 antibody 4.8 ug or mouse IgG or Flag-M2 , 4C ON, immunoprecipitates collected on magnesium beads (1-2 h 4C), immunoprecipitates washed and de-crosslinked, chromatin processed for quantitative PCR analysis (Figure 6B).
• Maraver A, Fernandez-Marcos PJ, Herranz D et al. Therapeutic Effect of Secretase Inhibition in Kras(G12V)-Driven Non-Small Cell Lung Carcinoma by Derepression of DUSP1 and Inhibition of ERK Cancer Cell 2012 Aug 14 [PMID: 22897852] (ChIP, Human)
• Wang CY, Wei Q, Han I et al. Hedgehog and Notch signaling regulate self-renewal of undifferentiated pleomorphic sarcomas. Cancer Res 2012 Feb 15 [PMID: 22232736]

FAQs for HES-1 Antibody (NBP1-47791). (Showing 1 - 1 of 1 FAQs).

1. Can you elaborate in detail regarding the specificity mentioned for NBP1-47791?
   • This antibody has been created to detect the human HES1 protein (UniProt Q14469). The immunogen information given states that the immunogen corresponds to amino acids 1-280 of human HES1, which according to the UniProt entry I've just linked to is the whole protein. I think the mention of Drosophila in the specificity information is referring to the derivation of the name of this protein. Wikipedia says, 'Transcription factor HES1 (hairy and enhancer of split-1) is a protein that is encoded by the Hes1 gene, and is the mammalian homolog of the hairy gene in Drosophila'.

Bioinformatics

• Gene Symbol: HES1
• Entrez:
  • 3280(Human)
• Uniprot:
  • Q14469()