FBXW7/Cdc4 Antibody

Western Blot: FBXW7/Cdc4 Antibody [NBP1-59631] - Sample Type: Human Fetal Heart Antibody Dilution: 1.0 ug/ml

Immunohistochemistry: FBXW7/Cdc4 Antibody [NBP1-59631] - Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue Observed Staining: Cytoplasmic in cell bodies and processes of pinealocytes Primary Antibody Concentration: 1:100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec

Western Blot: FBXW7/Cdc4 Antibody [NBP1-59631] - Jurkat cell lysate.

Western Blot: FBXW7/Cdc4 Antibody [NBP1-59631] - Analysis of 721_B whole cell lysates, Antibody Dilution: 1 ug/ml.

Western Blot: FBXW7/Cdc4 Antibody [NBP1-59631] - Analysis of 721_B whole cell lysates, Antibody Dilution: 1.0 ug/ml.

Western Blot: FBXW7/Cdc4 Antibody [NBP1-59631] - Analysis of 721_B whole cell lysates, Antibody Dilution: 1.0 ug/ml.

Immunohistochemistry: FBXW7/Cdc4 Antibody [NBP1-59631] - Kidney

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

FBXW7/Cdc4 Antibody Summary

• Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human FBXW7/Cdc4. Peptide Sequence LKTGEFIRNLVTLESGGSGGVVWRIRASNTKLVCAVGSRNGTEETKLLVL. The peptide sequence for this immunogen was taken from within the described region.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FBXW7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1.0 ug/ml
• Application Notes: This is a rabbit polyclonal antibody against FBXW7 and was validated on Western blot.
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25822340).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 2% Sucrose
• Preservative: 0.09% Sodium Azide
• Purity: Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for FBXW7/Cdc4 Antibody

• AGO
• AGOFBXO30
• Archipelago Homolog
• archipelago, Drosophila, homolog of
• Cdc4
• CDC4FLJ16457
• F-box and WD repeat domain containing 7
• F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila)
• F-box and WD-40 domain-containing protein 7
• F-box protein FBW7
• F-box protein FBX30
• F-box protein SEL-10
• F-box/WD repeat-containing protein 7
• FBW6
• Fbw7
• FBW7hCdc4
• FBX30
• FBX30FLJ11071
• FBXO30
• FBXW6
• FBXW7
• hAgo
• homolog of C elegans sel-10
• SCF(Fbw7)
• SEL10
• SEL-10DKFZp686F23254
• SEL10FBW6

Background

FBXW7 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in FBXW7 gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Three transcript variants encoding three different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FBXW7/Cdc4 Antibody (NBP1-59631)(4)

Publications using NBP1-59631

• Liu J, Shi H, Li X et al. miR-223-3p regulates cell growth and apoptosis via FBXW7 suggesting an oncogenic role in human testicular germ cell tumors. Int. J. Oncol. 2016-12-15 [PMID: 28000896] (WB, Human)
• Marathe S, Liu S, Brai E et al. Notch signaling in response to excitotoxicity induces neurodegeneration via erroneous cell cycle reentry Cell Death Differ. 2015-03-27 [PMID: 25822340] (ICC/IF, IF/IHC, Mouse)
• Tseng YY, Moriarity BS, Gong W et al. PVT1 dependence in cancer with MYC copy-number increase. Nature 2014-08-07 [PMID: 25043044] (WB)
• Lu A, Pfeffer SR. et al. Golgi-associated RhoBTB3 targets Cyclin E for ubiquitylation and promotes cell cycle progression. J Cell Biol. 2013-10-28 [PMID: 24145166] (WB, Human)

Review for FBXW7/Cdc4 Antibody (NBP1-59631) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 02/10/2017

Summary

• Application: Western Blot
• Sample Tested: b cell leukemia,B cell lymphoma,Jurkat human acute T cell leukemia cell line
• Species: Human

Bioinformatics

• Gene Symbol: FBXW7
• Entrez:
  • 55294(Human)
• Uniprot:
  • Q969H0-4(Human)