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FBXW7/Cdc4 Antibody

 (4 Publications)
 (1 Review)
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Western Blot: FBXW7/Cdc4 Antibody [NBP1-59631] - Sample Type: Human Fetal Heart Antibody Dilution: 1.0 ug/ml
Western Blot: FBXW7/Cdc4 Antibody [NBP1-59631] - Sample Type: Human Fetal Heart Antibody Dilution: 1.0 ug/ml
Immunohistochemistry: FBXW7/Cdc4 Antibody [NBP1-59631] - Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue Observed Staining: Cytoplasmic in cell bodies and processes of pinealocytes Primary Antibody ...read more
Western Blot: FBXW7/Cdc4 Antibody [NBP1-59631] - Jurkat cell lysate.
Western Blot: FBXW7/Cdc4 Antibody [NBP1-59631] - Analysis of 721_B whole cell lysates, Antibody Dilution: 1 ug/ml.
Western Blot: FBXW7/Cdc4 Antibody [NBP1-59631] - Analysis of 721_B whole cell lysates, Antibody Dilution: 1.0 ug/ml.
Western Blot: FBXW7/Cdc4 Antibody [NBP1-59631] - Analysis of 721_B whole cell lysates, Antibody Dilution: 1.0 ug/ml.
Immunohistochemistry: FBXW7/Cdc4 Antibody [NBP1-59631] - Kidney

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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FBXW7/Cdc4 Antibody Summary

Immunogen
Synthetic peptides corresponding to FBXW7(F-box and WD repeat domain containing 7) The peptide sequence was selected from the C terminal of FBXW7 (NP_001013433). Peptide sequence LKTGEFIRNLVTLESGGSGGVVWRIRASNTKLVCAVGSRNGTEETKLLVL.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FBXW7
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.2-1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Immunocytochemistry
Application Notes
This is a rabbit polyclonal antibody against FBXW7 and was validated on Western blot.
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
FBXW7/Cdc4 Lysate (NBP2-65025)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-59631 in the following applications:
Publications
Read Publications using
NBP1-59631 in the following applications:

  • ICC/IF
    1 publication
  • IHC
    1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for FBXW7/Cdc4 Antibody

  • AGO
  • AGOFBXO30
  • Archipelago Homolog
  • archipelago, Drosophila, homolog of
  • Cdc4
  • CDC4FLJ16457
  • F-box and WD repeat domain containing 7
  • F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila)
  • F-box and WD-40 domain-containing protein 7
  • F-box protein FBW7
  • F-box protein FBX30
  • F-box protein SEL-10
  • F-box/WD repeat-containing protein 7
  • FBW6
  • Fbw7
  • FBW7hCdc4
  • FBX30
  • FBX30FLJ11071
  • FBXO30
  • FBXW6
  • FBXW7
  • hAgo
  • homolog of C elegans sel-10
  • SCF(Fbw7)
  • SEL10
  • SEL-10DKFZp686F23254
  • SEL10FBW6

Background

FBXW7 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in FBXW7 gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Three transcript variants encoding three different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of Argonaute 2 on different lysates using anti-Argonaute 2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7
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Western Blot: Notch-1 Antibody (mN1A) [NB100-78486] - Cell extracts from Jurkat (Lane 1) or mouse thymocytes (Lane 2) were Western Blot analyzed with monoclonal anti-NOTCH1 antibody. The mN1A antibody recognizes both mouse and human 270 kD full-length NOTCH1 and 110-120 kD cleaved NOTCH 1 (NICD).
Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (mN1A) [NB100-78486] - The Notch1 Antibody was tested in HEK293 cells at a 1:50 dilution against Dylight 488 (Green). Actin was counterstained against Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue).
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Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Western blot analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Immunofluorescence analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: c-jun Antibody (5B1) [NBP2-37576] - Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using c-Jun mouse mAb with DAB staining.
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Western Blot: MUL1 Antibody [NBP2-31363] - Western blot analysis of K562 (A), Rt4 (B), and SH-SY5Y (C) cell lysate using MUL1 antibody at a concentration of 2 ug/ml.
Immunocytochemistry/Immunofluorescence: MUL1 Antibody [NBP2-31363] - MUL1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody concentration of 0.1 ug/ml was used.
Western Blot: MUL1 Antibody [NBP2-31363] - WB analysis of MUL1 protein in lysate of human liver and partial recombinant MUL1 protein by using the primary antibody concentration of 2ug/ml and 0.5 ug/ml respectively.
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Western Blot: ALDH9A1 Antibody [NBP1-89150] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
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Western Blot: ALDH9A1 Antibody [NBP1-89150] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
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Western Blot: RISC Antibody [NBP1-87127] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
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Publications for FBXW7/Cdc4 Antibody (NBP1-59631)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


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(3)
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Review for FBXW7/Cdc4 Antibody (NBP1-59631) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-59631:
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Images Ratings Applications Species Date Details
Western Blot FBXW7/Cdc4 NBP1-59631
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reviewed by:
JAEWOONG LEE
 WB Human 02/10/2017
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Summary

ApplicationWestern Blot
Sample TestedB cell lymphoma,Jurkat human acute T cell leukemia cell line,b cell leukemia
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol
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FAQs for FBXW7/Cdc4 Antibody (NBP1-59631) (0)

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Positive Control Lysate(s)

FBXW7/Cdc4 Knockout 293T Cell Lysate

Secondary Antibodies

 

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Diseases for FBXW7/Cdc4 Antibody (NBP1-59631)

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Western Blot FBXW7/Cdc4 NBP1-59631
Enlarge
 JAEWOONG LEE
02/10/2017
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol FBXW7
Entrez
Uniprot