Recombinant Human Jagged 2 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Jagged 2 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 0.5-2 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Jagged 2 protein
Human Jagged 2
(Met27-Leu1082)
Accession #AAB61285
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Met27
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
JAG2
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
138.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
165-175 kDa, reducing conditions
Publications
Read Publications using
1726-JG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Jagged 2 Fc Chimera Protein, CF

  • HJ2
  • JAG2
  • Jagged 2
  • Jagged2
  • protein jagged-2
  • SER2

Background

Human Jagged 2 is a 131 kDa (predicted) member of the Delta-Serrate-Lag-2 (DSL) family of ligands. This family activates LIN12/Notch proteins and thereby regulates cell fate determination during development (1 - 5). It is a type 1 transmembrane protein that is synthesized as a 1238 amino acid (aa) precursor (SwissProt # Q9Y219). It contains a 23 aa signal sequence, a large 1057 aa extracellular region, a 21 aa transmembrane region, and a short 137 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats (many of which are also sites of calcium binding), a von Willebrand factor (vWF) type C domain, and a cysteine-rich region just proximal to the transmembrane segment (2). There are two isoforms for human Jagged 2, named long and short. The short form lacks a splicing variant region (aa 421 - 461) that is present in the long form of the protein. Human Jagged 2 shares 90% and 87% aa sequence identity with mouse and rat Jagged 2, respectively. During murine embryonic development, Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (2). In 2-week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (2). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (2). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems, T cell lineage differentiation, natural killer cells, and the establishment of functional natural killer cell lines (3, 5, 6).

  1. Shawber, C. et al. (1996) Dev. Biol. 180:370.
  2. Luo, B. et al. (1997) Mol. Cell. Biol. 17:6057.
  3. Valsecchi, V. et al. (1997) Mech. Dev. 69:203.
  4. Schickwann, T. et al. (2000) Blood 96:950.
  5. DeHart, S. et al. (2005) Blood 105:3521.
  6. de La Coste, A. and A.A. Freitas (2006) Immunol. Lett. 102:1.

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Publications for Jagged 2 (1726-JG)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Binding Assay, Bioassay.


Filter By Application
Binding Assay
(1)
Bioassay
(6)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using 1726-JG Applications Species
D Zhang, X Qiao, L Wang, L Zhang, J Yao, X Wu, T Yu, KI Boström, Y Yao Skip is essential for Notch signaling to induce Sox2 in cerebral arteriovenous malformations Cell. Signal., 2020;68(0):109537. 2020 [PMID: 31927035] (Bioassay, Human) Bioassay Human
M López-Guer, S Xargay-Tor, P Fuentes, J Roldán, B González-F, L Rosich, E Silkensted, MJ García-Leó, E Lee-Vergés, N Giménez, A Giró, M Aymerich, N Villamor, J Delgado, A López-Guil, XS Puente, E Campo, ML Toribio, D Colomer Specific NOTCH1 antibody targets DLL4-induced proliferation, migration, and angiogenesis in NOTCH1-mutated CLL cells Oncogene, 2019;0(0):. 2019 [PMID: 31616059] (Bioassay, Human) Bioassay Human
M Colombo, S Garavelli, M Mazzola, N Platonova, D Giannandre, R Colella, L Apicella, M Lancellott, E Lesma, S Ancona, MT Palano, M Barbieri, E Taiana, E Lazzari, A Basile, M Turrini, A Pistocchi, A Neri, R Chiaramont Multiple myeloma exploits Jagged1 and Jagged2 to promote intrinsic and bone marrow-dependent drug resistance Haematologica, 2019;0(0):. 2019 [PMID: 31582544] (Bioassay, Human) Bioassay Human
R Schwentner, G Jug, MO Kauer, T Schnöller, P Waidhofer-, W Holter, C Hutter JAG2 signaling induces differentiation of CD14+ monocytes into Langerhans cell histiocytosis-like cells J. Leukoc. Biol., 2019;105(1):101-111. 2019 [PMID: 30296338] (Binding Assay, Human) Binding Assay Human
Diaz B, Yuen A, Iizuka S, Higashiyama S, Courtneidge S Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia. J Cell Biol, 2013;201(2):279-92. 2013 [PMID: 23589494] (Bioassay, Human) Bioassay Human
Sundlisaeter E, Edelmann R, Hol J, Sponheim J, Kuchler A, Weiss M, Udalova I, Midwood K, Kasprzycka M, Haraldsen G The alarmin IL-33 is a notch target in quiescent endothelial cells. Am J Pathol, 2012;181(3):1099-111. 2012 [PMID: 22809957] (Bioassay, Human) Bioassay Human
Erceg S, Lukovic D, Moreno-Manzano V, Stojkovic M, Bhattacharya S Derivation of cerebellar neurons from human pluripotent stem cells. Curr Protoc Stem Cell Biol, 2012;20(0):1H.51. 2012 [PMID: 22415839] (Bioassay, Human) Bioassay Human

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Blogs on Jagged 2.

Notch Antibody Proves Metastatic Lung Cancer Has a Jagged Edge
We at Novus Biologicals are one of the leading antibody suppliers for cancer research. In a recent Notch antibody study, the Notch ligand Jagged 2 was found to promote the growth of metastatic lung cancer cells by inhibiting miR-200, which blocks epit...  Read full blog post.

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Bioinformatics

Gene Symbol JAG2
Uniprot