Recombinant Human Jagged 1 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Jagged 1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 0.5-2.0 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Jagged 1 protein
Human Jagged 1
(Ser32-Ser1046)
Accession # P78504
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Ser32
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
JAG1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
137 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
170-180 kDa, under reducing conditions
Publications
Read Publications using
1277-JG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Jagged 1 Fc Chimera Protein, CF

  • AGS
  • AHDMGC104644
  • Alagille syndrome
  • AWS
  • CD339 antigen
  • CD339
  • HJ1
  • JAG1
  • Jagged 1
  • Jagged1
  • JAGL1
  • protein jagged-1

Background

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region (SwissProt # P78504). The ECD contains a DSL domain (aa 185-229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1-8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9-10).

  1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
  2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
  3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
  4. Lai, E.C. (2004) Development 131:965.
  5. Milner, L.A. et al. (1994) Blood 83:2057.  
  6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504
  7. Linheng, L. et al. (1998) Immunity 8:43.
  8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
  9. Oda, T. et al. (1997) Nat. Genet. 16:235.
  10. Oda, T. et al. (1997) Genomics 43:376.

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Publications for Jagged 1 (1277-JG)(27)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 3 applications: Binding Assay, Bioassay, In Vivo.


Filter By Application
Binding Assay
(1)
Bioassay
(25)
In Vivo
(1)
All Applications
Filter By Species
Human
(23)
Mouse
(3)
N/A
(1)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using 1277-JG Applications Species
SH Chung, W Shen, KC Davidson, A Pébay, RCB Wong, B Yau, M Gillies Differentiation of Retinal Glial Cells From Human Embryonic Stem Cells by Promoting the Notch Signaling Pathway Front Cell Neurosci, 2019;13(0):527. 2019 [PMID: 31849614] (Bioassay, Human) Bioassay Human
M López-Guer, S Xargay-Tor, P Fuentes, J Roldán, B González-F, L Rosich, E Silkensted, MJ García-Leó, E Lee-Vergés, N Giménez, A Giró, M Aymerich, N Villamor, J Delgado, A López-Guil, XS Puente, E Campo, ML Toribio, D Colomer Specific NOTCH1 antibody targets DLL4-induced proliferation, migration, and angiogenesis in NOTCH1-mutated CLL cells Oncogene, 2019;0(0):. 2019 [PMID: 31616059] (Bioassay, Human) Bioassay Human
S Matsui, M Ochiai, K Yasuda, SI Mae, M Kotaka, T Toyoda, T Yamamoto, K Osafune Differentiation and isolation of iPSC-derived remodeling ductal plate-like cells by use of an AQP1-GFP reporter human iPSC line Stem Cell Res, 2019;35(0):101400. 2019 [PMID: 30735882] (Bioassay, Human) Bioassay Human
S Stephenson, MA Care, I Fan, A Zougman, DR Westhead, GM Doody, RM Tooze Growth Factor-like Gene Regulation Is Separable from Survival and Maturation in Antibody-Secreting Cells J. Immunol., 2019;202(4):1287-1300. 2019 [PMID: 30642980] (Bioassay, Human) Bioassay Human
R Schwentner, G Jug, MO Kauer, T Schnöller, P Waidhofer-, W Holter, C Hutter JAG2 signaling induces differentiation of CD14+ monocytes into Langerhans cell histiocytosis-like cells J. Leukoc. Biol., 2019;105(1):101-111. 2019 [PMID: 30296338] (Binding Assay, Human) Binding Assay Human
SY Lee, F Long Notch signaling suppresses glucose metabolism in mesenchymal progenitors to restrict osteoblast differentiation J. Clin. Invest., 2018;0(0):. 2018 [PMID: 30284985] (Bioassay, Mouse) Bioassay Mouse
J Manokawinc, P Nattasit, T Thongngam, P Pavasant, KA Tompkins, H Egusa, T Osathanon RNA sequencing data of Notch ligand treated human dental pulp cells Data Brief, 2018;17(0):407-413. 2018 [PMID: 29876410] (Bioassay, Human) Bioassay Human
CJM Antila, V Rraklli, HA Blomster, KM Dahlström, TA Salminen, J Holmberg, L Sistonen, C Sahlgren Sumoylation of Notch1 represses its target gene expression during cell stress Cell Death Differ., 2018;0(0):. 2018 [PMID: 29305585] (Bioassay, Human) Bioassay Human
DW Youngstrom, R Senos, RL Zondervan, JD Brodeur, AR Lints, DR Young, TL Mitchell, ME Moore, MH Myers, WJ Tseng, KM Loomes, KD Hankenson Intraoperative delivery of the Notch ligand Jagged-1 regenerates appendicular and craniofacial bone defects NPJ Regen Med, 2017;2(0):32. 2017 [PMID: 29302365] (In Vivo, Mouse) In Vivo Mouse
T Gocha, BM Rao, R DasGupta Identification and characterization of a novel Sso7d scaffold-based binder against Notch1 Sci Rep, 2017;7(1):12021. 2017 [PMID: 28931897] (Bioassay, Human) Bioassay Human
Show All 27 Publications.

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Bioinformatics

Gene Symbol JAG1
Uniprot
COVID-19 update