Recombinant Human Jagged 1 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Jagged 1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 0.5-2.0 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Jagged 1 protein
Human Jagged 1
(Ser32-Ser1046)
Accession # P78504
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Ser32
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
JAG1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
137 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
170-180 kDa, under reducing conditions
Publications
Read Publications using
1277-JG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Jagged 1 Fc Chimera Protein, CF

  • AGS
  • AHDMGC104644
  • Alagille syndrome
  • AWS
  • CD339 antigen
  • CD339
  • HJ1
  • JAG1
  • Jagged 1
  • Jagged1
  • JAGL1
  • protein jagged-1

Background

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region (SwissProt # P78504). The ECD contains a DSL domain (aa 185-229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1-8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9-10).

  1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
  2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
  3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
  4. Lai, E.C. (2004) Development 131:965.
  5. Milner, L.A. et al. (1994) Blood 83:2057.  
  6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504
  7. Linheng, L. et al. (1998) Immunity 8:43.
  8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
  9. Oda, T. et al. (1997) Nat. Genet. 16:235.
  10. Oda, T. et al. (1997) Genomics 43:376.

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Publications for Jagged 1 (1277-JG)(37)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 3 applications: Binding Assay, Bioassay, In Vivo.


Filter By Application
Binding Assay
(1)
Bioassay
(35)
In Vivo
(1)
All Applications
Filter By Species
Human
(32)
Mouse
(4)
N/A
(1)
All Species
Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using 1277-JG Applications Species
K Zohorsky, S Lin, K Mequanint Immobilization of Jagged1 Enhances Vascular Smooth Muscle Cells Maturation by Activating the Notch Pathway Cells, 2021;10(8):. 2021 [PMID: 34440858] (Bioassay, Human) Bioassay Human
Y Wang, J Chang, ZQ Wang, Y Li Sirt3 promotes the autophagy of HK?2 human proximal tubular epithelial cells via the inhibition of Notch?1/Hes?1 signaling Molecular Medicine Reports, 2021;24(3):. 2021 [PMID: 34278469] (Bioassay, Human) Bioassay Human
Z Zhao, X Shen, D Zhang, H Xiao, H Kong, B Yang, L Yang miR-153 enhances the therapeutic effect of radiotherapy by targeting JAG1 in pancreatic cancer cells Oncology Letters, 2021;21(4):300. 2021 [PMID: 33732376] (Bioassay, Human) Bioassay Human
S Shimizu, K Yoshioka, S Aki, Y Takuwa Class II phosphatidylinositol 3-kinase-C2&amp;alpha is essential for Notch signaling by regulating the endocytosis of &amp;gamma-secretase in endothelial cells Scientific Reports, 2021;11(1):5199. 2021 [PMID: 33664344] (Bioassay, Human) Bioassay Human
K Uchimura, H Wu, Y Yoshimura, BD Humphreys Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling Cell Reports, 2020;33(11):108514. 2020 [PMID: 33326782] (Bioassay, Human) Bioassay Human
S Kakuda, RK Lopilato, A Ito, RS Haltiwange Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2 J. Biol. Chem., 2020;0(0):. 2020 [PMID: 32820046] (Bioassay, Human) Bioassay Human
J Manokawinc, P Sumrejkanc, L Boonprakon, P Pavasant, H Egusa, T Osathanon NOTCH2 participates in Jagged1-induced osteogenic differentiation in human periodontal ligament cells Sci Rep, 2020;10(1):13329. 2020 [PMID: 32770090] (Bioassay, Human) Bioassay Human
J Fan, W Shen, SR Lee, AE Mathai, R Zhang, G Xu, MC Gillies Targeting the Notch and TGF-&amp;beta signaling pathways to prevent retinal fibrosis in vitro and in vivo Theranostics, 2020;10(18):7956-7973. 2020 [PMID: 32724452] (Bioassay, Human) Bioassay Human
MR Batista, P Diniz, A Torres, D Murta, L Lopes-da-C, E Silva Notch signaling in mouse blastocyst development and hatching BMC Dev. Biol., 2020;20(1):9. 2020 [PMID: 32482162] (Bioassay, Mouse) Bioassay Mouse
SH Chung, W Shen, KC Davidson, A Pébay, RCB Wong, B Yau, M Gillies Differentiation of Retinal Glial Cells From Human Embryonic Stem Cells by Promoting the Notch Signaling Pathway Front Cell Neurosci, 2019;13(0):527. 2019 [PMID: 31849614] (Bioassay, Human) Bioassay Human
Show All 37 Publications.

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Bioinformatics

Gene Symbol JAG1
Uniprot