Recombinant Human Jagged 1 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Jagged 1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 0.5-2.0 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Jagged 1 protein
Human Jagged 1
(Ser32-Ser1046)
Accession # P78504
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Ser32
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
JAG1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
137 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
170-180 kDa, under reducing conditions
Publications
Read Publications using
1277-JG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Jagged 1 Fc Chimera Protein, CF

  • AGS
  • AHDMGC104644
  • Alagille syndrome
  • AWS
  • CD339 antigen
  • CD339
  • HJ1
  • JAG1
  • Jagged 1
  • Jagged1
  • JAGL1
  • protein jagged-1

Background

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region (SwissProt # P78504). The ECD contains a DSL domain (aa 185-229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1-8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9-10).

  1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
  2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
  3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
  4. Lai, E.C. (2004) Development 131:965.
  5. Milner, L.A. et al. (1994) Blood 83:2057.  
  6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504
  7. Linheng, L. et al. (1998) Immunity 8:43.
  8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
  9. Oda, T. et al. (1997) Nat. Genet. 16:235.
  10. Oda, T. et al. (1997) Genomics 43:376.

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1277-JG
Species: Hu
Applications: Bioactivity

Publications for Jagged 1 (1277-JG)(19)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(19)
All Applications
Filter By Species
Human
(17)
Mouse
(1)
N/A
(1)
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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using 1277-JG Applications Species
CJM Antila, V Rraklli, HA Blomster, KM Dahlström, TA Salminen, J Holmberg, L Sistonen, C Sahlgren Sumoylation of Notch1 represses its target gene expression during cell stress Cell Death Differ., 2018;0(0):. 2018 [PMID: 29305585] (Bioassay, Human) Bioassay Human
T Gocha, BM Rao, R DasGupta Identification and characterization of a novel Sso7d scaffold-based binder against Notch1 Sci Rep, 2017;7(1):12021. 2017 [PMID: 28931897] (Bioassay, Human) Bioassay Human
X Hong, A Margariti, A Le Bras, L Jacquet, W Kong, Y Hu, Q Xu Transdifferentiated Human Vascular Smooth Muscle Cells are a New Potential Cell Source for Endothelial Regeneration Sci Rep, 2017;7(1):5590. 2017 [PMID: 28717251] (Bioassay, Human) Bioassay Human
Z Liu, C Li, X Meng, Y Bai, J Qi, J Wang, Q Zhou, W Zhang, X Zhang Hypoxia-inducible factor-l? mediates aggrecan and collagen ? expression via NOTCH1 signaling in nucleus pulposus cells during intervertebral disc degeneration Biochem. Biophys. Res. Commun., 2017;0(0):. 2017 [PMID: 28526405] (Bioassay, Human) Bioassay Human
M Ciria, NA García, I Ontoria-Ov, H González-K, R Carrero, JL De La Pomp, JA Montero, P Sepúlveda Mesenchymal Stem Cell Migration and Proliferation Are Mediated by Hypoxia-Inducible Factor-1? Upstream of Notch and SUMO Pathways Stem Cells Dev., 2017;0(0):. 2017 [PMID: 28520516] (Bioassay, Human) Bioassay Human
Jagged1 Instructs Macrophage Differentiation in Leprosy PLoS Pathog, 2016;12(8):e1005808. 2016 [PMID: 27532668] (Bioassay, Human) Bioassay Human
Rouch J, Scott A, Lei N, Solorzano-Vargas R, Wang J, Hanson E, Kobayashi M, Lewis M, Stelzner M, Dunn J, Eckmann L, Martin M Development of Functional Microfold (M) Cells from Intestinal Stem Cells in Primary Human Enteroids. PLoS ONE, 2016;11(1):e0148216. 2016 [PMID: 26820624] (Bioassay, Human) Bioassay Human
Procopio M, Laszlo C, Al Labban D, Kim D, Bordignon P, Jo S, Goruppi S, Menietti E, Ostano P, Ala U, Provero P, Hoetzenecker W, Neel V, Kilarski W, Swartz M, Brisken C, Lefort K, Dotto G Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation. Nat Cell Biol, 2015;17(9):1193-204. 2015 [PMID: 26302407] (Bioassay, Human) Bioassay Human
Shao, Shan, Zhao, Xiaoai, Zhang, Xiaojin, Luo, Minna, Zuo, Xiaoxiao, Huang, Shangke, Wang, Ying, Gu, Shanzhi, Zhao, Xinhan Notch1 signaling regulates the epithelial-mesenchymal transition and invasion of breast cancer in a Slug-dependent manner. Mol Cancer, 2015;14(1):28. 2015 [PMID: 25645291] (Bioassay, Human) Bioassay Human
Ghiabi P, Jiang J, Pasquier J, Maleki M, Abu-Kaoud N, Halabi N, Guerrouahen B, Rafii S, Rafii A Breast cancer cells promote a notch-dependent mesenchymal phenotype in endothelial cells participating to a pro-tumoral niche. J Transl Med, 2015;13(0):27. 2015 [PMID: 25623554] (Bioassay, Human) Bioassay Human
Show All 19 Publications.

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Bioinformatics

Gene Symbol JAG1
Entrez
Uniprot