Notch-4 Antibody [Unconjugated]

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Notch‑4 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Goat Anti-Human Notch‑4 Intracellular Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3847) at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Notch-4 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Notch-4 Intracellular Domain
Gly1778-Lys2003
Accession # Q99466
Specificity
Detects human Notch-4 Intracellular Domain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) Notch-1 Intracellular Domain and rhNotch-2 Intracellular Domain is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NOTCH4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF3847 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Notch-4 Antibody [Unconjugated]

  • INT3
  • neurogenic locus notch homolog protein 4
  • notch 4
  • Notch homolog 4
  • NOTCH3
  • Notch4
  • Notch-4

Background

Notch-4 is a 250 kDa heterodimeric membrane protein with a C-terminal transmembrane fragment covalently linked to an N-terminal extracellular fragment. It functions as a receptor for transmembrane ligands such as the Jagged and Delta proteins. Upon ligand binding, it undergoes sequential proteolytic processing to release the intracellular domain (NICD), which is translocated to the nucleus where it activates target genes. In the region used as immunogen, human and mouse Notch-4 share 78% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Notch-4 Antibody (AF3847)(2)

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Video Protocols

WB Video Protocol

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mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol NOTCH4
Uniprot