DLL4 Antibody [Unconjugated]

Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DLL4 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

DLL4 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

DLL4 was detected in immersion fixed paraffin-embedded sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing vasculature. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Mu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

DLL4 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse DLL4
  Ser28-Pro525
  Accession # BAB18580
• Specificity: Detects mouse DLL4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human DLL4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DLL4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DLL4 Antibody [Unconjugated]

• Delta 4 precursor
• delta 4
• delta ligand 4
• delta4
• delta-like 4 (Drosophila)
• delta-like 4 homolog (Drosophila)
• delta-like 4 homolog
• delta-like 4 protein
• delta-like protein 4
• DLL4
• Drosophila Delta homolog 4
• hdelta2
• MGC126344
• notch ligand delta-2
• notch ligand DLL4

Background

Delta-Like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal Regulated Intramembrane Proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by A Disintegrin Metalloprotease (ADAM) and then by Presenilin/ gamma -Ssecretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1).

1. Shutter, J.R. et al. (2000) Genes Dev. 14:1313.
2. Iso, Tatsuya et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543.
3. Walker, L. et al. (2001) Stem Cells 19:543.
4. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113.
5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
6. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DLL4 Antibody (AF1389)(29)

Publications using AF1389

• Q Hou, J Jia, J Lin, L Zhu, S Xie, Q Yu, Y Li Bacillus subtilis programs the differentiation of intestinal secretory lineages to inhibit Salmonella infection Cell Reports, 2022;40(13):111416. 2022 [PMID: 36170821] (Western Blot, Mouse)
• C Xu, VV Dinh, K Kruse, HW Jeong, EC Watson, S Adams, F Berkenfeld, M Stehling, SJ Rasouli, R Fan, R Chen, I Bedzhov, Q Chen, K Kato, ME Pitulescu, RH Adams Induction of osteogenesis by bone-targeted Notch activation Elife, 2022;11(0):. 2022 [PMID: 35119364] (Western Blot, Mouse)
• E Azzoni, V Frontera, G Anselmi, C Rode, C James, EM Deltcheva, AS Demian, J Brown, C Barone, A Patelli, JR Harman, M Nicholls, SJ Conway, E Morrissey, SEW Jacobsen, DB Sparrow, AL Harris, T Enver, MFTR de Bruijn The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition Cell Reports, 2021;37(11):110103. 2021 [PMID: 34910918] (IHC, Mouse)
• DH Yeom, YS Lee, I Ryu, S Lee, B Sung, HB Lee, D Kim, JH Ahn, E Ha, YS Choi, SH Lee, WK You ABL001, a Bispecific Antibody Targeting VEGF and DLL4, with Chemotherapy, Synergistically Inhibits Tumor Progression in Xenograft Models International Journal of Molecular Sciences, 2020;22(1):. 2020 [PMID: 33383646] (IHC, Mouse)
• G Sharma, JM Jagtap, AK Parchur, VR Gogineni, S Ran, C Bergom, SB White, MJ Flister, A Joshi Heritable modifiers of the tumor microenvironment influence nanoparticle uptake, distribution and response to photothermal therapy Theranostics, 2020;10(12):5368-5383. 2020 [PMID: 32373218] (IHC, Rat)
• C Viallard, C Audiger, N Popovic, N Akla, K Lanthier, I Legault-Na, H Melichar, S Costantino, S Lesage, B Larrivée BMP9 signaling promotes the normalization of tumor blood vessels Oncogene, 2020;0(0):. 2020 [PMID: 32042114] (Western Blot, Human)
• R Fu, WC Lv, Y Xu, MY Gong, XJ Chen, N Jiang, Y Xu, QQ Yao, L Di, T Lu, LM Wang, R Mo, ZQ Wu Endothelial ZEB1 promotes angiogenesis-dependent bone formation and reverses osteoporosis Nat Commun, 2020;11(1):460. 2020 [PMID: 31974363] (IHC, Mouse)
• D Yasuda, D Kobayashi, N Akahoshi, T Ohto-Nakan, K Yoshioka, Y Takuwa, S Mizuno, S Takahashi, S Ishii Lysophosphatidic acid-induced YAP/TAZ activation promotes developmental angiogenesis by repressing Notch ligand Dll4 J. Clin. Invest., 2019;130(0):. 2019 [PMID: 31335323] (IHC, Mouse)
• S Payne, M Gunadasa-R, A Neal, AN Redpath, J Patel, KM Chouliaras, I Ratnayaka, N Smart, S De Val Regulatory pathways governing murine coronary vessel formation are dysregulated in the injured adult heart Nat Commun, 2019;10(1):3276. 2019 [PMID: 31332177] (IHC-Fr, Mouse)
• Z Gong, B Shang, Y Chu, X Chen, Q Li, K Liu, Y Chen, Y Huang, Y Han, Q Shang, Z Zheng, L Song, Y Li, R Liu, C Xu, X Zhang, B Liu, L Wang, C Shao, Y Wang, Y Shi Fibrotic liver microenvironment promotes Dll4 and SDF-1-dependent T-cell lineage development Cell Death Dis, 2019;10(6):440. 2019 [PMID: 31165736] (IHC, Human)
• M Fernández-, V Casquero-G, W Luo, F Francesca, S Ferreira R, S Del Olmo-C, R Benedito iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications Nat Commun, 2019;10(1):2262. 2019 [PMID: 31118412] (IHC-F, Mouse)
• A Neal, S Nornes, S Payne, MD Wallace, M Fritzsche, P Louphrasit, RN Wilkinson, KM Chouliaras, K Liu, K Plant, R Sholapurka, I Ratnayaka, W Herzog, G Bond, T Chico, G Bou-Ghario, S De Val Venous identity requires BMP signalling through ALK3 Nat Commun, 2019;10(1):453. 2019 [PMID: 30692543] (IHC, Mouse)
• F Tetzlaff, MG Adam, A Feldner, I Moll, A Menuchin, J Rodriguez-, D Sprinzak, A Fischer MPDZ promotes DLL4-induced Notch signaling during angiogenesis Elife, 2018;7(0):. 2018 [PMID: 29620522] (Western Blot, Mouse)
• AV Shah, GM Birdsey, C Peghaire, ME Pitulescu, NP Dufton, Y Yang, I Weinberg, L Osuna Alma, L Payne, JC Mason, H Gerhardt, RH Adams, AM Randi The endothelial transcription factor ERG mediates Angiopoietin-1-dependent control of Notch signalling and vascular stability Nat Commun, 2017;8(0):16002. 2017 [PMID: 28695891] (Western Blot, Mouse)
• RB Davis, DO Kechele, ES Blakeney, JB Pawlak, KM Caron Lymphatic deletion of calcitonin receptor-like receptor exacerbates intestinal inflammation JCI Insight, 2017;2(6):e92465. 2017 [PMID: 28352669] (ICC, Mouse)
• Toru Nakata, Hiromichi Shimizu, Sayaka Nagata, Go Ito, Satoru Fujii, Kohei Suzuki, Ami Kawamoto, Fumiaki Ishibashi, Reiko Kuno, Sho Anzai, Tatsuro Murano, Tomohiro Mizutani, Shigeru Oshima, Kiichiro Tsuchiya, Tetsuya Nakamura, Katsuto Hozumi, Mamoru Watanabe, Ryuichi Okamoto Data showing proliferation and differentiation of intestinal epithelial cells under targeted depletion of Notch ligands in mouse intestine. Data in Brief, 2017;0(0):2352-3409. 2017 [PMID: 28124021] (IHC, Mouse)
• Blood flow controls bone vascular function and osteogenesis Nat Commun, 2016;7(0):13601. 2016 [PMID: 27922003] (IHC, Mouse)
• Vijaykumar Chennupati Notch Signaling Regulates the Homeostasis of Tissue-Restricted Innate-like T Cells J Immunol, 2016;0(0):. 2016 [PMID: 27324132] (IHC, Mouse)
• Zhou Y, Williams J, Smallwood P, Nathans J Sox7, Sox17, and Sox18 Cooperatively Regulate Vascular Development in the Mouse Retina. PLoS ONE, 2015;10(12):e0143650. 2015 [PMID: 26630461] (IHC-Fr, Mouse)
• Gopinathan G, Milagre C, Pearce O, Reynolds L, Hodivala-Dilke K, Leinster D, Zhong H, Hollingsworth R, Thompson R, Whiteford J, Balkwill F Interleukin-6 Stimulates Defective Angiogenesis. Cancer Res, 2015;75(15):3098-107. 2015 [PMID: 26081809] (IHC-P, Human)
• Rama N, Dubrac A, Mathivet T, Ni Charthaigh R, Genet G, Cristofaro B, Pibouin-Fragner L, Ma L, Eichmann A, Chedotal A Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization. Nat Med, 2015;21(5):483-91. 2015 [PMID: 25894826] (IHC, Mouse)
• Zou M, Luo H, Xiang M Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord. Dev Dyn, 2015;244(1):86-97. 2015 [PMID: 25179941] (IHC, Mouse)
• Samarelli A, Riccitelli E, Bizzozero L, Silveira T, Seano G, Pergolizzi M, Vitagliano G, Cascone I, Carpentier G, Bottos A, Primo L, Bussolino F, Arese M Neuroligin 1 induces blood vessel maturation by cooperating with the alpha6 integrin. J Biol Chem, 2014;289(28):19466-76. 2014 [PMID: 24860089] (IHC, Mouse)
• Ramasamy S, Kusumbe A, Wang L, Adams R Endothelial Notch activity promotes angiogenesis and osteogenesis in bone. Nature, 2014;507(7492):376-80. 2014 [PMID: 24647000] (IHC-Fr, Mouse)
• Izumi N, Helker C, Ehling M, Behrens A, Herzog W, Adams RH Fbxw7 controls angiogenesis by regulating endothelial notch activity. PLoS ONE, 2012;7(7):e41116. 2012 [PMID: 22848434] (IHC, Mouse)
• Benedito R, Rocha S, Woeste M, Zamykal M, Radtke F, Casanovas O, Duarte A, Pytowski B, Adams R Notch-dependent VEGFR3 upregulation allows angiogenesis without VEGF-VEGFR2 signalling. Nature, 2012;484(7392):110-4. 2012 [PMID: 22426001] (IHC, Mouse)
• Stamataki D, Holder M, Hodgetts C Delta1 expression, cell cycle exit, and commitment to a specific secretory fate coincide within a few hours in the mouse intestinal stem cell system. PLoS ONE, 2011;6(9):e24484. 2011 [PMID: 21915337] (IHC-Fr, Mouse)
• Haller BK, Brave A, Wallgard E, Roswall P, Sunkari VG, Mattson U, Hallengard D, Catrina SB, Hellstrom M, Pietras K Therapeutic efficacy of a DNA vaccine targeting the endothelial tip cell antigen delta-like ligand 4 in mammary carcinoma. Oncogene, 2010;29(30):4276-86. 2010 [PMID: 20498640] (Western Blot, Human)
• Tammela T, Zarkada G, Wallgard E, Murtomaki A, Suchting S, Wirzenius M, Waltari M, Hellstrom M, Schomber T, Peltonen R, Freitas C, Duarte A, Isoniemi H, Laakkonen P, Christofori G, Yla-Herttuala S, Shibuya M, Pytowski B, Eichmann A, Betsholtz C, Alitalo K Blocking VEGFR-3 suppresses angiogenic sprouting and vascular network formation. Nature, 2008;454(7204):656-60. 2008 [PMID: 18594512] (IHC-Fr, Mouse)

Review for DLL4 Antibody (AF1389) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous - ICC Mouse 01/05/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: See PMID 23091063
• Species: Mouse

Bioinformatics

• Gene Symbol: DLL4
• Uniprot:
  • BAB18580()