DLL4 Antibody

Images

 
Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) followed by ...read more
DLL4 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) at 10 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

DLL4 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse DLL4
Ser28-Pro525
Accession # BAB18580
Specificity
Detects mouse DLL4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human DLL4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DLL4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 3
using
AF1389 in the following applications:

Publications
Read Publications using
AF1389 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DLL4 Antibody

  • Delta 4 precursor
  • delta 4
  • delta ligand 4
  • delta4
  • delta-like 4 (Drosophila)
  • delta-like 4 homolog (Drosophila)
  • delta-like 4 homolog
  • delta-like 4 protein
  • delta-like protein 4
  • DLL4
  • Drosophila Delta homolog 4
  • hdelta2
  • MGC126344
  • notch ligand delta-2
  • notch ligand DLL4

Background

Delta-Like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal Regulated Intramembrane Proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by A Disintegrin Metalloprotease (ADAM) and then by Presenilin/ gamma -Ssecretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1).

  1. Shutter, J.R. et al. (2000) Genes Dev. 14:1313.
  2. Iso, Tatsuya et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543.
  3. Walker, L. et al. (2001) Stem Cells 19:543.
  4. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113.
  5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
  6. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-78486
Species: Hu, Mu, Rt(-)
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, KO
NB100-93551
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB100-1533
Species: Hu, Mu, Po, Sh
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
AF3970
Species: Mu, Rt
Applications: WB, Flow, IHC, CyTOF-ready
NBP2-38770
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-89522
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84310
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MAB1144
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NBP1-47791
Species: Hu, Mk
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
H00004854-M01
Species: Hu
Applications: WB, ELISA
NBP2-27152
Species: Mu
Applications: WB
H00008209-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
NBP2-03523
Species: Hu, Ca
Applications: WB, Flow
NBP2-13961
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-47645
Species: Hu, Mu, Rt
Applications: Flow, IHC-P, PAGE, IF
NBP2-57809
Species: Hu
Applications: ICC/IF
AF1190
Species: Rt
Applications: WB, Flow, IHC, Block, CyTOF-ready
AF1389
Species: Mu
Applications: WB, ICC/IF

Publications for DLL4 Antibody (AF1389)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 9 applications: ICC, IHC, IHC - Not specified, IHC Fresh, IHC Frozen, IHC OCT-embedded, IHC Paraffin-embedded, IHC-Frozen, WB.


Filter By Application
ICC
(1)
IHC
(4)
IHC - Not specified
(2)
IHC Fresh
(1)
IHC Frozen
(2)
IHC OCT-embedded
(1)
IHC Paraffin-embedded
(1)
IHC-Frozen
(1)
WB
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(14)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using AF1389 Applications Species
F Tetzlaff, MG Adam, A Feldner, I Moll, A Menuchin, J Rodriguez-, D Sprinzak, A Fischer MPDZ promotes DLL4-induced Notch signaling during angiogenesis Elife, 2018;7(0):. 2018 [PMID: 29620522] (WB,, Mouse) WB Mouse
AV Shah, GM Birdsey, C Peghaire, ME Pitulescu, NP Dufton, Y Yang, I Weinberg, L Osuna Alma, L Payne, JC Mason, H Gerhardt, RH Adams, AM Randi The endothelial transcription factor ERG mediates Angiopoietin-1-dependent control of Notch signalling and vascular stability Nat Commun, 2017;8(0):16002. 2017 [PMID: 28695891] (WB,, Mouse) WB Mouse
RB Davis, DO Kechele, ES Blakeney, JB Pawlak, KM Caron Lymphatic deletion of calcitonin receptor-like receptor exacerbates intestinal inflammation JCI Insight, 2017;2(6):e92465. 2017 [PMID: 28352669] (ICC,, Mouse) ICC Mouse
Blood flow controls bone vascular function and osteogenesis Nat Commun, 2016;7(0):13601. 2016 [PMID: 27922003] (IHC,, Mouse) IHC Mouse
Vijaykumar Chennupati Notch Signaling Regulates the Homeostasis of Tissue-Restricted Innate-like T Cells J Immunol, 2016;0(0):. 2016 [PMID: 27324132] (IHC OCT-embedded,, Mouse) IHC OCT-embedded Mouse
Zhou Y, Williams J, Smallwood P, Nathans J Sox7, Sox17, and Sox18 Cooperatively Regulate Vascular Development in the Mouse Retina. PLoS ONE, 2015;10(12):e0143650. 2015 [PMID: 26630461] (IHC-Frozen,, Mouse) IHC-Frozen Mouse
Gopinathan G, Milagre C, Pearce O, Reynolds L, Hodivala-Dilke K, Leinster D, Zhong H, Hollingsworth R, Thompson R, Whiteford J, Balkwill F Interleukin-6 Stimulates Defective Angiogenesis. Cancer Res, 2015;75(15):3098-107. 2015 [PMID: 26081809] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Rama N, Dubrac A, Mathivet T, Ni Charthaigh R, Genet G, Cristofaro B, Pibouin-Fragner L, Ma L, Eichmann A, Chedotal A Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization. Nat Med, 2015;21(5):483-91. 2015 [PMID: 25894826] (IHC - Not specified,, Mouse) IHC - Not specified Mouse
Zou M, Luo H, Xiang M Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord. Dev Dyn, 2015;244(1):86-97. 2015 [PMID: 25179941] (IHC,, Mouse) IHC Mouse
Samarelli A, Riccitelli E, Bizzozero L, Silveira T, Seano G, Pergolizzi M, Vitagliano G, Cascone I, Carpentier G, Bottos A, Primo L, Bussolino F, Arese M Neuroligin 1 induces blood vessel maturation by cooperating with the alpha6 integrin. J Biol Chem, 2014;289(28):19466-76. 2014 [PMID: 24860089] (IHC,, Mouse) IHC Mouse
Show All 16 Publications.

Review for DLL4 Antibody (AF1389) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF1389:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ICC Mouse 01/05/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 23091063
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23091063

Details

Detection NotesSee PMID 23091063

Comments

CommentsPublished See PMID 23091063

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DLL4 Antibody (AF1389) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for DLL4 Antibody (AF1389)

Discover related pathways, diseases and genes to DLL4 Antibody (AF1389). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DLL4 Antibody (AF1389)

Discover more about diseases related to DLL4 Antibody (AF1389).
 

Pathways for DLL4 Antibody (AF1389)

View related products by pathway.

PTMs for DLL4 Antibody (AF1389)

Learn more about PTMs related to DLL4 Antibody (AF1389).

Blogs on DLL4.

New DLL4 Vaccine May Prevent Breast Tumors
We at Novus Biologicals have a large antibody database devoted to signalling pathways. These underpin every area of molecular biological research, including cancer. Among our cell signalling antibodies is one targeted to DLL4 (Delta-like protein 4). D...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

3
5
0
4
0
3
1
2
0
1
0

 
Anonymous
01/05/2015
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol DLL4
Entrez
Uniprot