DLL4 Antibody [Unconjugated]


Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) followed by ...read more
DLL4 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) at 10 µg/mL for 3 hours at room temperature. Cells ...read more
DLL4 was detected in immersion fixed paraffin-embedded sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) at 5 µg/mL for 1 hour at ...read more

Product Details

Reactivity MuSpecies Glossary
Applications WB, IHC, ICC/IF

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DLL4 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse DLL4
Accession # BAB18580
Detects mouse DLL4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human DLL4 is observed.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL
Reviewed Applications
Read 1 Review rated 3
AF1389 in the following applications:

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AF1389 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DLL4 Antibody [Unconjugated]

  • Delta 4 precursor
  • delta 4
  • delta ligand 4
  • delta4
  • delta-like 4 (Drosophila)
  • delta-like 4 homolog (Drosophila)
  • delta-like 4 homolog
  • delta-like 4 protein
  • delta-like protein 4
  • DLL4
  • Drosophila Delta homolog 4
  • hdelta2
  • MGC126344
  • notch ligand delta-2
  • notch ligand DLL4


Delta-Like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal Regulated Intramembrane Proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by A Disintegrin Metalloprotease (ADAM) and then by Presenilin/ gamma -Ssecretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1).

  1. Shutter, J.R. et al. (2000) Genes Dev. 14:1313.
  2. Iso, Tatsuya et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543.
  3. Walker, L. et al. (2001) Stem Cells 19:543.
  4. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113.
  5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
  6. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DLL4 Antibody (AF1389)(29)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ICC, IHC, IHC-F, IHC-Fr, IHC-P, Western Blot.

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Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using AF1389 Applications Species
Q Hou, J Jia, J Lin, L Zhu, S Xie, Q Yu, Y Li Bacillus subtilis programs the differentiation of intestinal secretory lineages to inhibit Salmonella infection Cell Reports, 2022;40(13):111416. 2022 [PMID: 36170821] (Western Blot, Mouse) Western Blot Mouse
C Xu, VV Dinh, K Kruse, HW Jeong, EC Watson, S Adams, F Berkenfeld, M Stehling, SJ Rasouli, R Fan, R Chen, I Bedzhov, Q Chen, K Kato, ME Pitulescu, RH Adams Induction of osteogenesis by bone-targeted Notch activation Elife, 2022;11(0):. 2022 [PMID: 35119364] (Western Blot, Mouse) Western Blot Mouse
E Azzoni, V Frontera, G Anselmi, C Rode, C James, EM Deltcheva, AS Demian, J Brown, C Barone, A Patelli, JR Harman, M Nicholls, SJ Conway, E Morrissey, SEW Jacobsen, DB Sparrow, AL Harris, T Enver, MFTR de Bruijn The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition Cell Reports, 2021;37(11):110103. 2021 [PMID: 34910918] (IHC, Mouse) IHC Mouse
DH Yeom, YS Lee, I Ryu, S Lee, B Sung, HB Lee, D Kim, JH Ahn, E Ha, YS Choi, SH Lee, WK You ABL001, a Bispecific Antibody Targeting VEGF and DLL4, with Chemotherapy, Synergistically Inhibits Tumor Progression in Xenograft Models International Journal of Molecular Sciences, 2020;22(1):. 2020 [PMID: 33383646] (IHC, Mouse) IHC Mouse
G Sharma, JM Jagtap, AK Parchur, VR Gogineni, S Ran, C Bergom, SB White, MJ Flister, A Joshi Heritable modifiers of the tumor microenvironment influence nanoparticle uptake, distribution and response to photothermal therapy Theranostics, 2020;10(12):5368-5383. 2020 [PMID: 32373218] (IHC, Rat) IHC Rat
C Viallard, C Audiger, N Popovic, N Akla, K Lanthier, I Legault-Na, H Melichar, S Costantino, S Lesage, B Larrivée BMP9 signaling promotes the normalization of tumor blood vessels Oncogene, 2020;0(0):. 2020 [PMID: 32042114] (Western Blot, Human) Western Blot Human
R Fu, WC Lv, Y Xu, MY Gong, XJ Chen, N Jiang, Y Xu, QQ Yao, L Di, T Lu, LM Wang, R Mo, ZQ Wu Endothelial ZEB1 promotes angiogenesis-dependent bone formation and reverses osteoporosis Nat Commun, 2020;11(1):460. 2020 [PMID: 31974363] (IHC, Mouse) IHC Mouse
D Yasuda, D Kobayashi, N Akahoshi, T Ohto-Nakan, K Yoshioka, Y Takuwa, S Mizuno, S Takahashi, S Ishii Lysophosphatidic acid-induced YAP/TAZ activation promotes developmental angiogenesis by repressing Notch ligand Dll4 J. Clin. Invest., 2019;130(0):. 2019 [PMID: 31335323] (IHC, Mouse) IHC Mouse
S Payne, M Gunadasa-R, A Neal, AN Redpath, J Patel, KM Chouliaras, I Ratnayaka, N Smart, S De Val Regulatory pathways governing murine coronary vessel formation are dysregulated in the injured adult heart Nat Commun, 2019;10(1):3276. 2019 [PMID: 31332177] (IHC-Fr, Mouse) IHC-Fr Mouse
Z Gong, B Shang, Y Chu, X Chen, Q Li, K Liu, Y Chen, Y Huang, Y Han, Q Shang, Z Zheng, L Song, Y Li, R Liu, C Xu, X Zhang, B Liu, L Wang, C Shao, Y Wang, Y Shi Fibrotic liver microenvironment promotes Dll4 and SDF-1-dependent T-cell lineage development Cell Death Dis, 2019;10(6):440. 2019 [PMID: 31165736] (IHC, Human) IHC Human
Show All 29 Publications.

Review for DLL4 Antibody (AF1389) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF1389:
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Images Ratings Applications Species Date Details
reviewed by:
Anonymous -
ICC Mouse 01/05/2015


Sample TestedSee PMID 23091063

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New DLL4 Vaccine May Prevent Breast Tumors
We at Novus Biologicals have a large antibody database devoted to signalling pathways. These underpin every area of molecular biological research, including cancer. Among our cell signalling antibodies is one targeted to DLL4 (Delta-like protein 4). D...  Read full blog post.

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Recent Reviews


Anonymous -
Application: ICC
Species: Mouse


Gene Symbol DLL4