csl/RBPJK Antibody

Images

 
Western Blot: csl/RBPJK Antibody [NBP1-33427] - Sample (30 ug of whole cell lysate) A: Jurkat 7.5% SDS PAGE diluted at 1:2000
Immunocytochemistry/ Immunofluorescence: csl/RBPJK Antibody [NBP1-33427] - Analysis of paraformaldehyde-fixed HeLa at 1:100 dilution.
Chromatin Immunoprecipitation (ChIP): csl/RBPJK Antibody [NBP1-33427] - Sheared, cross-linked chromatin was prepared from Jurkat T-ALL cells using the Covaris TruChIP kit and shearing with the LE220 sonicator. The ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RM, XpSpecies Glossary
Applications WB, ICC/IF, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

csl/RBPJK Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human RBPJK. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Mouse (97%), Rat (98%), Xenopus (93%), Rhesus Macaque (99%), Bovine (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RBPJ
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:1000
  • Western Blot 1:500 - 1:3000
Application Notes
Chip reactivity per customer review.
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP1-33427 in the following applications:

Publications
Read Publication using NBP1-33427.

Reactivity Notes

Xenopus laevis (93%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol (pH7)
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for csl/RBPJK Antibody

  • CBF1
  • CBF-1
  • CSL
  • H-2K binding factor-2
  • IGKJRB1
  • IGKJRB1RBPSUHCBF1
  • IGKJRBRBP-J kappa
  • immunoglobulin kappa J region recombination signal binding protein 1
  • J kappa-recombination signal-binding protein
  • KBF2
  • RBPJ
  • RBP-JK
  • RBPJKMGC61669
  • RBP-Jrecombining binding protein suppressor of hairless (Drosophila)
  • RBPSUH
  • recombination signal binding protein for immunoglobulin kappa J region
  • recombining binding protein suppressor of hairless
  • Renal carcinoma antigen NY-REN-30
  • SUH
  • suppressor of hairless homolog

Background

Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate. determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some Notch protein, it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for csl/RBPJK Antibody (NBP1-33427)(1)

Review for csl/RBPJK Antibody (NBP1-33427) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-33427:
Filter by Applications
ChIP
(1)
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Images Ratings Applications Species Date Details
Chromatin Immunoprecipitation csl/RBPJK NBP1-33427
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3
reviewed by:
Christopher Cardinale
ChIP Human 07/12/2018
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Summary

ApplicationChromatin Immunoprecipitation
Sample Testedsheared cross-linked chromatin
SpeciesHuman
Lot40114

Comments

CommentsSheared, cross-linked chromatin was prepared from Jurkat T-ALL cells using the Covaris TruChIP kit and shearing with the LE220 sonicator. The chromatin buffer was adjusted to 150 mM NaCl, 20 mM Tris-Cl pH 8, 2 mM EDTA, and 1% Triton X-100. The three indicated antibodies, as well as pure pre-immune rabbit IgG control, were added to each IP reaction, consisting of 7 million cells, and incubated overnight with mixing at 4 C. The antibodies were captured with Magna ChIP Protein A + G beads (Millipore), washed with three wash buffers, and the DNA eluted and purified with the iPure v2 kit (Diagenode). The purified DNA was normalized to the input control with qPCR quantification of the insulin exon 1 gene (INS) and an RBPJ binding site in the promoter of HES1.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for csl/RBPJK Antibody (NBP1-33427) (0)

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Secondary Antibodies

 

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Recent Reviews

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Christopher Cardinale
07/12/2018
Application: ChIP
Species: Human

Bioinformatics

Gene Symbol RBPJ
Entrez
Uniprot