NLRP3/NALP3 Antibody (25N10E9)

Images

 
Western Blot: NLRP3/NALP3 Antibody (25N10E9) [NBP2-03948] - Analysis of Raw 264 cell lysate using NLRP3/NALP3 antibody (NBP2-03948) at 2 ug/ml.
Western Blot: NLRP3/NALP3 Antibody (25N10E9) [NBP2-03948] - Analysis using the NALP3 monoclonal antibody. (A), (B), mouse RAW lysate (C) and the recombinant mouse NLRP3 NOD domain protein immunogen (D) probed with 2 ...read more
Simple Western: NLRP3/NALP3 Antibody (25N10E9) [NBP2-03948] - Simple Western lane view shows a specific band for NALP3 in 0.5 mg/ml of RAW lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western
Clone
25N10E9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Catalog# & Conjugate Size Price

NLRP3/NALP3 Antibody (25N10E9) Summary

Immunogen
A recombinant protein corresponding to the NOD domain* of murine NLRP3/NALP3 (amino acids 216-385) was used as the immunogen for this antibody. *Each region of NLRP3 has a different function. Oligomerization of NOD is required for formation of the NLRP3 inflammasome. The epitope that I25N10E9 binds to the NOD region has not been mapped.
Specificity
This antibody will not detect human NLRP3.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NLRP3
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 2-5 ug/ml
  • Simple Western 1:100
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Reactivity Notes

Human and Mouse

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for NLRP3/NALP3 Antibody (25N10E9)

  • AGTAVPRL
  • AII
  • AII/AVP
  • Angiotensin/vasopressin receptor AII/AVP-like
  • AVP
  • C1orf7
  • Caterpiller protein 1.1
  • CIAS1
  • CLR1.1
  • Cold autoinflammatory syndrome 1 protein
  • Cold autoinflammatory syndrome 1
  • Cryopyrin
  • Familial cold autoinflammatory syndrome
  • FCAS
  • FCU
  • Leucine-rich repeat-, and PYD-containing protein 3
  • Muckle-Wells syndrome
  • MWS
  • NACHT
  • NACHT, LRR and PYD containing protein 3
  • NACHT, LRR and PYD domains-containing protein 3
  • NALP3
  • NLR family, pyrin domain containing 3
  • NLRP3 monoclonal
  • NLRP3
  • Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3
  • PYPAF1
  • PYRIN-containing APAF1-like protein 1

Background

NLRP3/NALP3 belongs to the NLR family of innate immune cytoplasmic receptors (reviewed in De Nardo and Latz, 2011; Kanneganti, 2010; Anand, 2011). NLR family members contain an N-terminus effector region consisting of either a pyrin (PYD), a CARD, or a BIR domain. NLRs also have an intermediate conserved NOD domain and a C-terminus LRR domain. NLRs, like TLRs, alert the immune system to the presence of PAMPs and DAMPs. Like a number of other NLRs, NLRP3 functions in a multiprotein complex called the inflammasome. The inclusion of a particular NLR within an inflammasome depends upon the upstream trigger. The NLRP3 inflammasome is considered to be a global sensor and assembles in response to a broad spectrum of PAMPs and DAMPs. For example, helmith, bacteria, plasmodium and viruses have all been shown to activate NLRP3 (reviewed in Menu and Vince, 2011). The PYD of activated NLRP3 associates with the PYD of ASC, triggering the CARD of ASC to associate with the CARD of caspase-1 and completes the assembly of the NLRP3 inflammasome. This serves as a platform for caspase-1 activation. Activation of NLRP3 can be inhibited by glybenclamide (also known as glyburide) (reviewed in Lamkanfi et al, 2009). Glybenclamide is an ATP-sensitive potassion channel inhibitor that has been shown to inhibit various biological processes including activation of the NRLP3 inflammasome. Glybenclamide is thought to act downstream of the P2X7 receptor but upsteam of NLRP3. Glybenclamide blocks activation of caspase-1 and IL-1beta K+ efflux inhibition. Glybenclamide has a number of additional functions besides blocking including enhancing insulin action and stimulating synthesis of glucose transporters. Researchers are encouraged to consult the published literature regarding additional information about how glybenclamide perturbs biological processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for NLRP3/NALP3 Antibody (NBP2-03948). (Showing 1 - 4 of 4 FAQ).

  1. Are the NLRP3/NALP3 antibodies validated in Simple Western?
    • Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.
  2. Does NLRP3/NALP3 antibodies come in lyophilized form?
    • Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.
  3. What is the theoretical molecular weight for NLRP3/NALP3 antibodies?
    • The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.
  4. What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?
    • A recombinant sequence of amino acids 216 - 385 within the NOD domain of murine NLRP3/NALP3.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

HRP Labeled NBP2-03948H

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Blogs on NLRP3/NALP3.

NLRP3/NALP3 - Sensing and responding to pathogen infection
The inflammasome is a multi protein complex that is an important component of the innate immune response. The inflammasome is able to sense and respond to pathogen infections by recognizing pathogen-associated molecular patterns and mediating the ...  Read full blog post.

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Bioinformatics

Gene Symbol NLRP3
Entrez
Uniprot