Niemann-Pick C1 Antibody

Images

 
Genetic Strategies: Knockout Validated: Niemann-Pick C1 Antibody [NB400-148] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NPC1 knockout (KO) HeLa cell line. PVDF ...read more
Western Blot: Niemann-Pick C1 Antibody [NB400-148] - Hela lysate, BHK lysate, A431 lysate tested at 1:1000. Image provided by verified customer review.
Immunocytochemistry/ Immunofluorescence: Niemann-Pick C1 Antibody [NB400-148] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Niemann-Pick C1 at 5 ug/ml ...read more
Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody [NB400-148] - Staining of human brain, cortex, neurons and astrocytes.
Western Blot: Niemann-Pick C1 Antibody [NB400-148] - Detection of human NPC1 in 20 ug of human fibroblast cell lysate.
Immunocytochemistry/ Immunofluorescence: Niemann-Pick C1 Antibody [NB400-148] - NPC1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei were counterstained with Dapi (blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, ChHa, PmSpecies Glossary
Applications WB, EM, ICC/IF, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Niemann-Pick C1 Antibody Summary

Immunogen
A synthetic peptide made to the C-terminal region of human Niemann-Pick C. [UniProt# O15118]
Localization
Late endosome and Lysosome membrane; Single-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NPC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:3000
  • Electron Microscopy
  • Immunocytochemistry/Immunofluorescence 1:250
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Knockout Validated
Application Notes
This Niemann-Pick C1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections,Western blot. In Western blot the antibody detects heterogeneously glycosylated NPC1 protein with prominent bands at 170 and 220 kDa. Results with mouse in Western blot have been mixed. It has also been tested for immuno-EM (on human protein only). Use in Electron Microscopy reported in scientific literature (PMID: 21051527). Use in Immunocytochemistry/immunofluorescence and western blot reported in scientific literature (PMID:24209575).
Control Peptide
Niemann-Pick C1 Peptide (NB400-148PEP)
Reviewed Applications
Read 3 Reviews rated 4.3
using
NB400-148 in the following applications:

Publications
Read Publications using
NB400-148 in the following applications:

  • EM
    1 publication
  • 9 publications
  • IHC
    1 publication
  • 1 publication
  • IP
    3 publications
  • WB
    18 publications

Reactivity Notes

Human, mouse, rat and Chinese hamster and primate (PMID 22212234). Porcine reactivity reported in scientific literature (PMID: 21051527) Results with mouse in Western blot have been mixed.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Niemann-Pick C1 Antibody

  • FLJ98532
  • Niemann-Pick C1 protein
  • Niemann-Pick disease, type C1
  • NPC
  • NPC1

Background

Niemann-Pick type C1 (NPC1) is a member of a family of genes encoding membrane-bound proteins containing putative sterol sensing domains. NPC1 protein regulates cholesterol transport from late endosomes-lysosomes to other intracellular compartments. NPC1 overexpression increases the rate of trafficking of low density lipoprotein cholesterol to the endoplasmic reticulum and the rate of delivery of endosomal cholesterol to the plasma membrane. NPC disease is an inherited neurovisceral lipid storage disorder of unesterified cholesterol accumulation in lysosomes. It is characterized by progressive neural and liver degeneration, resulting from inactivating mutations in NPC1, in most cases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Niemann-Pick C1 Antibody (NB400-148)(33)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chinese Hamster, Hamster, Porcine.

We have publications tested in 6 applications: EM, ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
EM
(1)
ICC/IF
(9)
IHC
(1)
IHC-P
(1)
IP
(3)
WB
(18)
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Filter By Species
Human
(15)
Mouse
(5)
Rat
(1)
Chinese Hamster
(3)
Hamster
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using NB400-148 Applications Species
Ebrahimi-Fakhari D, Wahlster L, Bartz F et al. Reduction of TMEM97 increases NPC1 protein levels and restores cholesterol trafficking in Niemann-pick type C1 disease cells Hum. Mol. Genet. 2016 Jul 04 [PMID: 27378690] (ICC/IF) ICC/IF
Ferrante A, De Nuccio C, Pepponi R et al. Stimulation of adenosine A2A receptors reduces intracellular cholesterol accumulation and rescues mitochondrial abnormalities in human neural cell models of Niemann-Pick C1. Neuropharmacology. 2015 Nov 26 [PMID: 26631535] (WB, Human) WB Human
Li J, Deffieu MS, Lee PL et al. Glycosylation inhibition reduces cholesterol accumulation in NPC1 protein-deficient cells. Proc. Natl. Acad. Sci. U.S.A. 2015 Nov 17 [PMID: 26578804] (WB, Chinese Hamster) WB Chinese Hamster
Kanerva K, Uronen RL, Blom T et al. LDL Cholesterol Recycles to the Plasma Membrane via a Rab8a-Myosin5b-Actin-Dependent Membrane Transport Route. Dev Cell. 2013 Nov 11 [PMID: 24209575] (IP, Human) IP Human
Armstrong A, Mattsson N, Appelqvist H et al. Lysosomal Network Proteins as Potential Novel CSF Biomarkers for Alzheimer's Disease. Neuromolecular Med. 2013 Oct 8 [PMID: 24101586] (WB, Human) WB Human
Cianciola NL, Greene DJ, Morton RE, Carlin CR. Adenovirus RIDalpha uncovers a novel pathway requiring ORP1L for lipid droplet formation independent of NPC1. Mol Biol Cell. 2013 Nov [PMID: 24025716] (ICC/IF, Human) ICC/IF Human
Roszell BR, Tao JQ, Yu KJ et al. Pulmonary Abnormalities in Animal Models Due to Niemann-Pick Type C1 (NPC1) or C2 (NPC2) Disease. PLoS One 2013 Jul 2 [PMID: 23843985] (WB, Mouse) WB Mouse
Fu R, Wassif CA, Yanjanin NM et al. Efficacy of N-acetylcysteine in phenotypic suppression of Mouse models of Niemann-Pick disease, type C1. Hum Mol Genet 2013 May 21 [PMID: 23666527] (WB, Mouse) WB Mouse
Goedeke L, Vales-Lara FM, Fenstermaker M et al. A Regulatory Role for MicroRNA 33(Asterisk) in Controlling Lipid Metabolism Gene Expression. Mol Cell Biol 2013 Jun [PMID: 23547260] (WB, Human) WB Human
Ohgane K, Karaki F, Dodo K, Hashimoto Y. Discovery of Oxysterol-Derived Pharmacological Chaperones for NPC1: Implication for the Existence of Second Sterol-Binding Site Chem Biol 2013 Mar 21 [PMID: 23521797] (WB, Human) WB Human
Show All 33 Publications.

Reviews for Niemann-Pick C1 Antibody (NB400-148) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NB400-148:
Filter by Applications
IP
(1)
WB
(2)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IP Human 12/23/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedSee PMID 24209575
SpeciesHuman

Blocking

Blocking DetailsSee PMID 24209575

Details

Detection NotesSee PMID 24209575

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 24209575.
 
reviewed by:
Anonymous
WB Human 12/23/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22607065
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22607065

Details

Detection NotesSee PMID 22607065

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 22607065.
Western Blot Niemann-Pick C1 NB400-148
Enlarge
reviewed by:
Anonymous
WB Human 02/28/2014
View

Summary

ApplicationWestern Blot
Sample TestedHela lysate, BHK lysate, A431 lysate
SpeciesHuman

Blocking

Blocking Details5% non-fat dry milk, 45 min r.t.

Primary Anitbody

Dilution Ratio1:1000; o/n, 4 °C, T-TBS, NFDM 3%

Product General Protocols

View specific protocols for Niemann-Pick C1 Antibody (NB400-148): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Niemann-Pick C1 Antibody (NB400-148). (Showing 1 - 4 of 4 FAQs).

  1. I'm looking for a Niemann-Pick type1 or 2 antibody to stain mouse heart section embedded in paraffin. I would like to label these sections with another primary antibody to show colocalisation of my protein of interest. I would like to know if you have a rabbit anti-Niemann-Pick antibody that works well for fluorescence immunohistochemistry?
    • Niemann-Pick C1 Antibody (<a href="http://www.novusbio.com/NB400-148" target="_self">NB400-148</a>) (0.1 ml) is one of our best sellers and this antibody has been cited in at least 17 peer reviewed research publications in journals of high repute. We have only one Niemann-Pick type C2 antibody with catalog # <a href="http://www.novusbio.com/H00010577-D01P" target="_self">H00010577-D01P</a>, but this antibody is yet to be established for IHC application. However, if you would like to try this antibody for IHC-P application you would qualify for our Innovators Reward program. Through this program if you complete an online review with image, detailing your positive or negative results we will send you a discount voucher for 100% of the purchase price of the reviewed product. Read more about <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a>.
  2. We have an inquiry of your product Cat. ID # NB400-148. Our customer would like to know the immunogen sequence of this product. If you cannot provide the detail, would you please check if this antibody covers amino acids 600-800?
    • This antibody was made to a sequence found within amino acids 1230-1278.
  3. Have you received any complaints on these lots F1 and G1 of NB400-148?
    • I have looked through our complaints and spoke to our lab manager, and we have not had any recent reported problems with these lots. These lots do happen to be approximately two years old, so it may be that they have lost reactivity over this time. Our antibody products are guaranteed for 6 months from date of receipt.
  4. I want to purchase NPC1 antibody from your company and the catalog is NB400-148. Could you please tell me in which precise position the antibody binds of the NPC1 protein?
    • NB400-148 binds between amino acids 1178 and 1278 of human Niemann-Pick C.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB400-148AF405
Alexa Fluor 488 Labeled NB400-148AF488
Alexa Fluor 647 Labeled NB400-148AF647
Alexa Fluor 700 Labeled NB400-148AF700
Biotin Labeled NB400-148B
DyLight 405 Labeled NB400-148V
DyLight 488 Labeled NB400-148G
DyLight 550 Labeled NB400-148R
DyLight 650 Labeled NB400-148C
FITC Labeled NB400-148F
HRP Labeled NB400-148H

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

 
Anonymous
12/23/2014
Application: IP
Species: Human

 
Anonymous
12/23/2014
Application: WB
Species: Human

Anonymous
02/28/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol NPC1
Entrez
Uniprot