Ran Antibody (8D1A6)

Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of HepG2 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of paraffin-embedded stomach cancer tissues using RAN mouse mAb with DAB staining.

Flow Cytometry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of Hela cells using RAN mouse mAb (green) and negative control (red).

Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against human RAN (AA: 1-216) recombinant protein. (Expected MW is 50.4 kDa)

Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mouse mAb against Hela (1), NIH/3T3 (2), A431 (3), C6 (4), Jurkat (5), Hela (6), COS7 (7), and Jurkat (8) cell lysate.

Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of GC-7901 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of Hela cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of paraffin-embedded cervical cancer tissues using RAN mouse mAb with DAB staining.

ELISA: Ran Antibody (8D1A6) [NBP2-61832] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
• Clone: 8D1A6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Ran Antibody (8D1A6) Summary

• Immunogen: Purified recombinant fragment of human RAN (AA: 1-216) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAN
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
• Theoretical MW: 24.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Ran Antibody (8D1A6)

• Androgen receptor-associated protein 24
• ARA24Gsp1
• GTPase Ran
• GTP-binding nuclear protein Ran
• guanosine triphosphatase Ran
• member RAS oncogene family
• OK/SW-cl.81
• RAN, member RAS oncogene family
• RanGTPase
• Ras-like protein TC4
• Ras-related nuclear protein
• TC4

Background

RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAN