Nup153 Antibody

Images

 
Western Blot: Nup153 Antibody [NB100-93329] - Detection of Human NUP153 on HeLa whole cell lysate using NB100-9329. NUP153 was also immunoprecipitated by rabbit anti-NUP153 antibody NB100-9330.
Immunocytochemistry: Nup153 Antibody [NB100-93329] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-NUP153 used at a dilution of 1:200 (1ug/ml). Detection: Red-fluorescent goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

Nup153 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human nucleoporin 153kDa using the numbering given in entry NP_005115.2 (GeneID 9972).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NUP153
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100 to 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-93329 in the following applications:

Publications
Read Publication using
NB100-93329 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nup153 Antibody

  • CG4453
  • dmNup153
  • dNup153
  • HNUP153
  • N153
  • nuclear pore complex protein hnup153,153 kDa nucleoporin
  • nuclear pore complex protein Nup153
  • nucleoporin 153kD
  • nucleoporin 153kDa
  • Nucleoporin Nup153
  • Nup 153
  • Nup153 Nucleoporin 153
  • nup153

Background

Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are pore complex-specific glycoproteins characterized by cytoplasmically oriented O-linked N-acetylglucosamine residues and numerous repeats of the pentapeptide sequence XFXFG. The protein encoded by this gene has three distinct domains: a N-terminal region within which a pore targeting domain has been identified, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nup153 Antibody (NB100-93329)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Filter By Species
Human
(1)
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Review for Nup153 Antibody (NB100-93329) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-93329:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 12/23/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23791529
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23791529

Details

Detection NotesSee PMID 23791529

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23791529.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nup153 Antibody (NB100-93329) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Nup153 Antibody (NB100-93329)

Discover related pathways, diseases and genes to Nup153 Antibody (NB100-93329). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Nup153 Antibody (NB100-93329)

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Blogs on Nup153.

NUP153 & 53BP1: A Novel DNA Repair Pathway
Mediating DNA damage is a crucial process, and one of the most important cellular guards against cancer. In response to DNA damage, sophisticated cellular machinery is recruited to repair the breaks, and if it fails, the cell is committed to death. De...  Read full blog post.

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Recent Reviews

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Anonymous
12/23/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol NUP153
Entrez
Uniprot