Nup153 Antibody

Western Blot: Nup153 Antibody [NB100-93329] - Knockdown of NUP153 in MDA-MB-231 breast cancer cells. Lane 1: ladder. Lane 2: Scramble Control Lane 3: NUP153 knockdown. Substantially reduced protein detection in knockdown sample and not in scramble control reflect antibody's unique specificity for Nup153. Image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: Nup153 Antibody [NB100-93329] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-NUP153 used at a dilution of 1:200 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG highly cross adsorbed Antibody used at a dilution of 1:100.

Western Blot: Nup153 Antibody [NB100-93329] - Detection of Human NUP153 on HeLa whole cell lysate using NB100-9329. NUP153 was also immunoprecipitated by rabbit anti-NUP153 antibody NB100-9330.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Nup153 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human nucleoporin 153kDa using the numbering given in entry NP_005115.2 (GeneID 9972).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NUP153
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100 to 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockout Validated
  • Western Blot 1:2000-1:10000
• Application Notes: Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. Nup153 antibody validated for WB from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Nup153 Antibody

• CG4453
• dmNup153
• dNup153
• HNUP153
• N153
• nuclear pore complex protein hnup153,153 kDa nucleoporin
• nuclear pore complex protein Nup153
• nucleoporin 153kD
• nucleoporin 153kDa
• Nucleoporin Nup153
• Nup 153
• Nup153 Nucleoporin 153
• nup153

Background

Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are pore complex-specific glycoproteins characterized by cytoplasmically oriented O-linked N-acetylglucosamine residues and numerous repeats of the pentapeptide sequence XFXFG. The protein encoded by this gene has three distinct domains: a N-terminal region within which a pore targeting domain has been identified, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nup153 Antibody (NB100-93329)(2)

Publications using NB100-93329

• Fiserova J, Maninova M, Sieger T et al. Nuclear pore protein TPR associates with lamin B1 and affects nuclear lamina organization and nuclear pore distribution Cell. Mol. Life Sci. 2019-02-14 [PMID: 30762072] (WB, Human)
• Wan G, Zhang X, Langley RR et al. DNA-Damage-Induced Nuclear Export of Precursor MicroRNAs Is Regulated by the ATM-AKT Pathway. Cell Rep 2013-06-27 [PMID: 23791529] (WB, Human)

Reviews for Nup153 Antibody (NB100-93329) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: David Feuersinger WB Human 01/31/2018

Summary

• Application: Western Blot
• Sample Tested: MDA-MB-231 nuclear extracts
• Species: Human
• Lot: A1

Comments

• Comments: Knockdown of NUP153 in MDA-MB-231 breast cancer cells. Lane 1: ladder. Lane 2: Scramble Control Lane 3: NUP153 knockdown. Substantially reduced protein detection in knockdown sample and not in scramble control reflect antibody's unique specificity for Nup153. This image was submitted by customer review.

reviewed by: cynthia morimoto WB Human 12/23/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 23791529
• Species: Human

Bioinformatics

• Gene Symbol: NUP153
• Entrez:
  • 9972(Human)
• Uniprot:
  • P49790(Human)