RanBP2 Antibody

Images

 
Western Blot: RanBP2 Antibody [NB100-93337] - Detection of Human RanBP2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Western Blot: RanBP2 Antibody [NB100-93337] - Detection of Human RanBP2 on HeLa whole cell lysate using NB100-93337. RanBP2 was also immunoprecipitated by rabbit anti-RanBP2 antibody NB100-93338.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

RanBP2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1050 and 1100 of human RAN binding protein 2 using the numbering given in entry NP_006258.3 (GeneID 5903).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RANBP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2 - 10 ug/mg lysate
Theoretical MW
358 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NB100-93337 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RanBP2 Antibody

  • ANE1
  • E3 SUMO-protein ligase RanBP2
  • EC 5.2.1.8
  • Nuclear pore complex protein Nup358
  • nucleoporin 358
  • Nucleoporin Nup358
  • NUP358TRP2
  • p270
  • RAN binding protein 2
  • Ran-binding protein 2
  • RanBP2
  • transformation-related protein 2,358 kDa nucleoporin
  • TRP1

Background

RAN is a small GTP-binding protein of the RAS superfamily that is associated with the nuclear membrane and is thought to control a variety of cellular functions through its interactions with other proteins. This gene encodes a very large RAN-binding protein that immunolocalizes to the nuclear pore complex. The protein is a giant scaffold and mosaic cyclophilin-related nucleoporin implicated in the Ran-GTPase cycle. The encoded protein directly interacts with the E2 enzyme UBC9 and strongly enhances SUMO1 transfer from UBC9 to the SUMO1 target SP100. These findings place sumoylation at the cytoplasmic filaments of the nuclear pore complex and suggest that, for some substrates, modification and nuclear import are linked events. This gene is partially duplicated in a gene cluster that lies in a hot spot for recombination on chromosome 2q. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RanBP2 Antibody (NB100-93337)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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(1)
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RANBP2
Entrez
Uniprot