hnRNP C1 + C2 Antibody (CL2593)

Images

 
Genetic Strategies: Western Blot: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HNRNPC antibody. ...read more
Immunocytochemistry/ Immunofluorescence: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining in U2OS cell line with Anti-HNRNPC monoclonal antibody, showing distinct nuclear (without nucleoli) staining in green. ...read more
Immunohistochemistry: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining of human testis shows nuclear positivity in seminiferous tubules.
Western Blot: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Lane 1: Marker [kDa]. Lane 2:Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining in HeLa cell line showing distinct nuclear (without nucleoli)Staining in green. Microtubule-staining and nuclear probes ...read more
Immunocytochemistry/ Immunofluorescence: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining of human cell line A431 showing distinct nuclear (without nucleoli)Staining in green. Microtubule-staining and nuclear ...read more
Immunocytochemistry/ Immunofluorescence: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining in U251 cell line showing distinct nuclear (without nucleoli)Staining in green. Microtubule-staining and nuclear probes ...read more
Immunocytochemistry/ Immunofluorescence: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining in MCF7 cell line with Anti-HNRNPC monoclonal antibody, showing distinct nuclear (without nucleoli) staining in green. ...read more
Immunohistochemistry: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining of human rectum shows strong nuclear immunoreactivity in glandular and lamina propria cells.
Immunohistochemistry: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining of human small intestine shows strong nuclear positivity in glandular epithelium and in lamina propria cells.
Immunohistochemistry: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining of human kidney shows strong nuclear immunoreactivity.
Immunohistochemistry: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining of human prostate shows strong nuclear positivity in glandular and connective tissue cells.
Immunohistochemistry: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining of human fallopian tube shows strong nuclear immunoreactivity.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clone
CL2593
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

hnRNP C1 + C2 Antibody (CL2593) Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:AEMYGSVTEHPSPSPLLSSS
Predicted Species
Mouse (95%). Backed by our 100% Guarantee.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HNRNPC
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Knockdown Validated
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, Fixation/Permeabilization with PFA/Triton X-100 is recommended.
Control Peptide
hnRNP C1 + C2 Recombinant Protein Antigen (NBP2-36776PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for hnRNP C1 + C2 Antibody (CL2593)

  • C1
  • C2
  • heterogeneous nuclear ribonucleoprotein C (C1/C2)
  • heterogeneous nuclear ribonucleoproteins C1/C2
  • hnRNP C1/C2
  • HNRNP
  • hnRNPC
  • HNRPC
  • MGC104306
  • MGC105117
  • MGC117353
  • MGC131677
  • nuclear ribonucleoprotein particle C1 protein
  • nuclear ribonucleoprotein particle C2 protein
  • SNRPC

Background

RNA polymerase II transcripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins are important in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA translation, and turnover. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts. When this is performed, the hnRNPs are isolated (consisting of protein groups named A to U), and many of these protein groups consist of more than one isoform. The major steadystate proteins of the isolated hnRNP complex are the A1, A2, B1, B2, C1, and C2 with a range of molecular weight starting with 34 kDa up to 43 kDa. The hnRNP-C proteins have a single RNP motif RNA-binding domain (RBD) of 80 to 100 amino acid long. The hnRNP -C proteins preferentially bind to uridine-rich RNA sequences. Oligomerization of the protein through leucine rich regions in its C-terminal end is important for RNA binding.4 Although its physiological action is unknown, mutation of the hnRNPC gene causes a embryonic lethal phenotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for hnRNP C1 + C2 Antibody (NBP2-36776) (0)

There are no publications for hnRNP C1 + C2 Antibody (NBP2-36776).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for hnRNP C1 + C2 Antibody (NBP2-36776) (0)

There are no reviews for hnRNP C1 + C2 Antibody (NBP2-36776). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for hnRNP C1 + C2 Antibody (NBP2-36776) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional hnRNP C1 + C2 Products

Research Areas for hnRNP C1 + C2 Antibody (NBP2-36776)

Find related products by research area.

Blogs on hnRNP C1 + C2

There are no specific blogs for hnRNP C1 + C2, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our hnRNP C1 + C2 Antibody (CL2593) and receive a gift card or discount.

Bioinformatics

Gene Symbol HNRNPC
Uniprot