Nbs1 [p Ser343] Antibody

Product Discontinued

Nbs1 [p Ser343] Antibody Summary

• Immunogen: Synthetic phosphopeptide derived from human Nibrin around the phosphorylation site of Serine 343.
• Modification: p Ser343
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NBN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of p-p95/NBS1 (S343) pAb in extracts from HeLa cells. Immunohistochemistry (IHC) analyzes of p-p95/NBS1 (S343) pAb in paraffin-embedded human tonsil tissue. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
• Theoretical MW: 85 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Nbs1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Nbs1 [p Ser343] Antibody

• ATV
• AT-V1
• AT-V2
• Cell cycle regulatory protein p95
• FLJ10155
• MGC87362
• NBN
• NBS
• Nbs1
• NBS1P95
• Nibrin
• Nijmegen breakage syndrome 1 (nibrin)
• Nijmegen breakage syndrome protein 1
• p95 protein of the MRE11/RAD50 complex
• p95

Background

NBS1 (Nijmegen breakage syndrome protein 1, Nibrin) is the eukaryotic component of MRN complex (Mre11-Rad50-Nbs1) involved in homologous recombination repair for DNA double-strand breaks (DSBs) and DNA damage-induced checkpoint activation. NBS1, a nuclear protein with a theoretical molecular weight of 65-85 kDa, is composed of 2 functional regions: the forkhead associated (FHA) N-terminal domain (24-83 amino acids) and the breast cancer C-terminus (BRCT) domain (105-181 amino acids). Mutations in the NBN gene are associated with Nijmegen breakage syndrome, characterized by microcephaly, growth retardation, immunodeficiency, and an increased susceptibility to prostate cancer, lung cancer, liver cancer and intrahepatic cholangiocarcinoma (IHC) (1).

In DNA double strand break repair, the FHA/BRCT domains bind DNA damage sensor proteins including gamma H2AX (phospho Ser139), phosphorylated MDC1 and CtIP (CtBP-interacting protein). NBS1 then recruits the other members of the MRN complex, Mre11 and Rad50, to the proximity of DNA DSBs. The MRN complex has also been shown to interact with ATM or ATR kinases in the presence of DSBs or replication fork stalling, respectively. Phosphorylation of NBS1 at Ser 278 and Ser343 in response to ionizing radiation (IR) is dependent on ATM and is important for activation of the S phase checkpoint. The activation of ATM in response to DNA damage is also facilitated by MRN and may lead to induction of apoptosis (2, 3).

References

1. Bian L, Meng Y, Zhang M, Li D. (2019) MRE11-RAD50-NBS1 complex alterations and DNA damage response: implications for cancer treatment. Mol Cancer. 26;18(1):169. PMID: 31767017

2. Zhang Y, Zhou J, Lim CU. (2006) The role of NBS1 in DNA double strand break repair, telomere stability, and cell cycle checkpoint control. Cell Res. 16(1):45-54. PMID: 16467875

3. Komatsu K. NBS1 and multiple regulations of DNA damage response. (2016) J Radiat Res. 57 Suppl 1:i11-i17. PMID: 27068998

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nbs1 Antibody (NB100-92610)(12)

Publications using NB100-92610

• Sessa G, GOmez-GonzAlez B, Silva S et al. BRCA2 promotes R-loop resolution by DDX5 helicase at DNA breaks to facilitate their repair by homologous recombination The EMBO journal 2021-02-26 [PMID: 33634895]
• Mano M, Ippodrino R, Zentilin L et al. Genome-wide RNAi screening identifies host restriction factors critical for in vivo AAV transduction Proc Natl Acad Sci U S A. 2015-09-07 [PMID: 26305933] (WB, Human)
• Ahmed-Seghir S, Pouvelle C, Despras E et al. Aberrant C-terminal domain of polymerase eta targets the functional enzyme to the proteosomal degradation pathway. DNA Repair (Amst.). 2015-02-26 [PMID: 25766642] (WB, Human)
• Wang Y, Hong Y, Li M et al. Mutation Inactivation of Nijmegen Breakage Syndrome Gene (NBS1) in Hepatocellular Carcinoma and Intrahepatic Cholangiocarcinoma. PLoS One 2013-12-13 [PMID: 24349281] (IF/IHC, WB, Human)
• Despras E, Delrieu N, Garandeau C et al. Regulation of the specialized DNA polymerase eta: revisiting the biological relevance of its PCNA- and ubiquitin-binding motifs Environ Mol Mutagen 2012-12-01 [PMID: 23076824] (WB, Human)
• Zimmermann M, Arachchige-Don AS, Donaldson MS et al. Elevated cyclin G2 expression intersects with DNA damage checkpoint signaling and is required for a potent G2/M checkpoint arrest response to doxorubicin. J Biol Chem 2012-06-29 [PMID: 22589537]
• Tentner AR, Lee MJ, Ostheimer GJ et al. Combined experimental and computational analysis of DNA damage signaling reveals context-dependent roles for Erk in apoptosis and G1/S arrest after genotoxic stress. Mol. Syst. Biol. 20128:568. [PMID: 22294094]
• Furusawa Y, Fujiwara Y, Campbell P et al. DNA double-strand breaks induced by cavitational mechanical effects of ultrasound in cancer cell lines. PLoS ONE 20127(1):e29012. [PMID: 22235259] (ICC/IF, Human)
• Bohgaki T, Bohgaki M, Cardoso R et al. Genomic instability, defective spermatogenesis, immunodeficiency, and cancer in a mouse model of the RIDDLE syndrome PLoS Genet 2011-04-01 [PMID: 21552324] (WB, Mouse)
• Takahashi A, Mori E, Ohnishi T. The foci of DNA double strand break-recognition proteins localize with gammaH2AX after heat treatment J Radiat Res 2010-01-01 [PMID: 20173316] (ICC/IF, Human)
• Townsend K, Mason H, Blackford AN et al. Mediator of DNA damage checkpoint 1 (MDC1) regulates mitotic progression. J Biol Chem 2009-12-01 [PMID: 19826003] (WB, Human)
• Takahashi A, Yamakawa N, Kirita T et al. DNA damage recognition proteins localize along heavy ion induced tracks in the cell nucleus J Radiat Res 2008-11-01 [PMID: 18987440] (ICC/IF, Human)

Reviews for Nbs1 Antibody (NB100-92610) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 12/22/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: primary human cells
• Species: Human

reviewed by: De-identified L WB Human 05/01/2014

Summary

• Application: Western Blot
• Sample Tested: HEK293 whole cell lysates
• Species: Human

FAQs for Nbs1 Antibody (NB100-92610). (Showing 1 - 4 of 4 FAQs).

1. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
   • Yes, if this product is used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product.
2. What research areas can this product be used in?
   • All Nbs1 products can be used in Breast Cancer, Cancer, Checkpoint signaling, Chromatin Research, DNA Double Strand Break Repair, DNA Repair, Homologous Recombination, Non-homologous end-joining, Tumor Suppressors, and Cell Cycle and Replication.
3. What is NBS1?
   • NBS1 is Nijmegen breakage syndrome protein 1, or Nibrin. Please refer to the background listed in the datasheet for a full summary of this common name.
4. What is the theoretical molecular weight of Nbs1 antibodies?
   • In general, there is only one isoform described for this target. The TMW of the human form of the target protein is 85kDa. The mouse form is 84kDa. However, the observed molecular weight may vary.

Bioinformatics

• Gene Symbol: NBN
• Entrez:
  • 4683(Human)
  • 85482(Rat)
• Uniprot:
  • O60934()