Cadherin-4/R-Cadherin Antibody

Images

 
Western blot shows lysates of mouse brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Cadherin‑4/R‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6677) followed ...read more
Cadherin‑4/R‑Cadherin was detected in immersion fixed frozen sections of embryonic mouse brain using Sheep Anti-Mouse Cadherin‑4/R‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6677) at 1.7 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Cadherin-4/R-Cadherin Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cadherin-4/R-Cadherin
His21-Ala731
Accession # P39038
Specificity
Detects mouse Cadherin-4/R-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Cadherin-4/R-Cadherin and recombinant mouse (rm) Cadherin-2 is observed and less than 1% cross-reactivity with rmE‑Cadherin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CDH4
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
AF6677 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cadherin-4/R-Cadherin Antibody

  • CAD4
  • cadherin 4, type 1, preproprotein
  • cadherin 4, type 1, R-cadherin (retinal)
  • Cadherin4
  • Cadherin-4
  • CDH4
  • FLJ22202
  • FLJ40547
  • MGC126700
  • MGC138355
  • RCAD
  • R-CAD
  • R-Cadherin
  • Retinal cadherin

Background

Cadherin-4 (also Retinal/R-Cadherin) is a 120 kDa (100 kDa predicted) member of the classical cadherin family of molecules. It is expressed on mammary epithelium, olfactory bulb neurons, cerebellar granule cells, vascular smooth muscle, astrocytes and pancreatic beta -cells. Cadherin-4 forms both homodimers and heterodimers with N-cadherin. These complexes will oligomerize, providing sources for adhesion and migration. Cadherin-4 also binds to KLRG1 expressed on NK, T and Treg lymphocytes. Mouse cadherin-4 proprecursor is 893 amino acids (aa) in length. It contains a 146 aa propeptide (aa 21-166) coupled to a 746 amino acid (aa) type I transmembrane mature protein (aa 167-913). The mature region possesses a 565 aa extracellular domain (aa 167-731) plus a 160 aa cytoplasmic region (aa 753-913). Consistent with classical cadherins, cadherin-4 contains five cadherin repeats in the extracellular domain (aa 167-721). There is one potential splice form that shows a 44 aa substitution for aa 291-913. Over aa 21-731, mouse cadherin-4 shares 98% and 93% aa identity with rat and human cadherin-4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cadherin-4/R-Cadherin Antibody (AF6677)(1)

We have publications tested in 1 confirmed species: Xenograft.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Xenograft
(1)
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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol CDH4
Uniprot