Anti-N-Cadherin Antibodies: Novus Biologicals

NBP1-48309

N-Cadherin Antibody (13A9) - BSA Free

(2 Reviews)

(42 Publications)

0.1 ml

NBP1-48309

Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of N-Cadherin expression in HeLa whole cell lysate.

N-Cadherin-Antibody-13A9-Immunocytochemistry-NBP1-48309-img0026.jpg

Flow (Intracellular): N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa with NBP1-48309 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody.

Simple Western: N-Cadherin Antibody (13A9) [NBP1-48309] - Simple Western lane view shows a specific band for N Cadherin in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

<b>Biological Strategies Validation. </b>N-Cadherin-Antibody-13A9-Western-Blot-NBP1-48309-img0032.jpg

Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - Adult mouse neural stem cells stained for N-Cadherin. Antibody at 1:100. ICC/IF image submitted by a verified customer review.

N-Cadherin-Antibody-13A9-Immunohistochemistry-NBP1-48309-img0031.jpg

Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on U-251 MG cells with N-Cadherin Antibody (13A9) NBP1-48309AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - HEK 293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti- at 5 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - IHC analysis of N Cadherin in mouse liver using DAB with hematoxylin counterstain.

Immunohistochemistry-Paraffin: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of a FFPE tissue section of human brain using 1:200 dilution of N-Cadherin antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa cells with NBP1-48309AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

N-Cadherin (13A9) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Mouse anti- N-Cadherin (13A9) Protein G Purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP1-48309AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

N-Cadherin (13A9) was detected in immersion fixed U-251 MG human glioblastoma cell line using Mouse anti-N-Cadherin (13A9) Protein G Purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NBP1-48309R) (red) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemical characterization of tissue, 2D-, and 3D-cultures. Nuclei are labeled with DAPI (blue) in all images. (A) Cryosection of an adult mouse heart immunostained for myomesin (red) and vimentin (green). (B) Confocal optical section on the substrate level of a spheroid made of hiPSC-CM and CF cultured for 1 month. Immunostaining for myosin heavy chain (red), and vimentin (green). (C) Confocal optical section above the substrate level of the same spheroid as shown in (B). (D) 2D-cultured cardiac fibroblasts immunostained for alpha-SMA (red) and all actin (green) shown as a maximum intensity projection of optical sections. (E) Single confocal optical sections perpendicular to the substrate through a CF-only, small spheroid (above) cultured for 5 days and immunostained for all actin (red) and alpha-SMA (green), and an optical section at midlevel (below). (F) Cryosection of fibroblast-only spheroid cultured for 3 weeks immunostained for vimentin (red) and alpha-SMA (green). (G) Western blot experiments and quantitative assessment of the ratio of alpha-SMA/vimentin (n = 4, ***p < 0.001) demonstrating the difference of alpha-SMA content in 2D vs. 3D cultures of pure CF. (H) Whole-mount immunostaining of a cardiomyocyte-only spheroid 1 month in culture, N-cadherin (red) and EH-myomesin (green). (I) Cardiomyocyte-only spheroid 1 month in culture, whole-mount immunostained for all-actin (red) and laminin (green). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32118040), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-48309AF350
Alexa Fluor 405	NBP1-48309AF405
Alexa Fluor 488	NBP1-48309AF488
Alexa Fluor 532	NBP1-48309AF532
Alexa Fluor 594	NBP1-48309AF594
Alexa Fluor 647	NBP1-48309AF647
Alexa Fluor 700	NBP1-48309AF700
Alexa Fluor 750	NBP1-48309AF750
Alexa Fluor Plus 405	NBP1-48309AFP405
Alexa Fluor Plus 488	NBP1-48309AFP488
Alexa Fluor Plus 555	NBP1-48309AFP555
Alexa Fluor Plus 594	NBP1-48309AFP594
Alexa Fluor Plus 647	NBP1-48309AFP647
Alexa Fluor Plus 680	NBP1-48309AFP680
Allophycocyanin	NBP1-48309APC
Allophycocyanin/Cy7	NBP1-48309APCCY7
Biotin	NBP1-48309B
CoraFluor 1	NBP1-48309CL1
DyLight 350	NBP1-48309UV
DyLight 405	NBP1-48309V
DyLight 488	NBP1-48309G
DyLight 550	NBP1-48309R
DyLight 594	NBP1-48309DL594
DyLight 650	NBP1-48309C
DyLight 680	NBP1-48309FR
DyLight 755	NBP1-48309IR
FITC	NBP1-48309F
HRP	NBP1-48309H
Janelia Fluor 525	NBP1-48309JF525
Janelia Fluor 549	NBP1-48309JF549
Janelia Fluor 646	NBP1-48309JF646
Janelia Fluor 669	NBP1-48309JF669
PE	NBP1-48309PE
PE/Atto594	NBP1-48309PEATT594
PE/Cy5.5	NBP1-48309PECY55
PE/Cy7	NBP1-48309PECY7
PerCP	NBP1-48309PCP
mFluor Violet 450 SE	NBP1-48309MFV450
mFluor Violet 500 SE	NBP1-48309MFV500
mFluor Violet 610 SE	NBP1-48309MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-48309SS
Azide and BSA Free	NBP2-80868

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AF6426

N-Cadherin Antibody [Unconjugated]

(30 Publications)

100 μg

AF6426

HAF016). A specific band was detected for N-Cadherin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and rat brain tissue. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for N-Cadherin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and rat brain tissue. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for N-Cadherin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

N-Cadherin was detected in immersion fixed A549 human lung carcinoma cell line using Sheep Anti-Human/Mouse/Rat N-Cadherin Affinity-purified Polyclonal Antibody (Catalog # AF6426) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # <a class=

NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intercalated disks between the myocardial cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

N-Cadherin was detected in immersion fixed paraffin-embedded sections of human heart using Sheep Anti-Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Sheep IgG VisUCyte HRP Polymer Antibody (Catalog # <a class=

VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intercalated disks between the myocardial cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

MAB13883) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox">

Recombinant Human N-Cadherin protein was serially diluted 2-fold and captured by Mouse Anti-Human N-Cadherin Monoclonal Antibody (Catalog # <a class=

MAB13883) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

5-001-A, open histogram), followed by Fluorescein-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0125). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

A172 cells were stained with Sheep Anti-Human/Mouse/Rat N‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426, filled histogram) or isotype control antibody (Catalog # <a class=

5-001-A, open histogram), followed by Fluorescein-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0125). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

<b>Biological Strategies Validation. </b>N-Cadherin Depletion Rescues the Migration Defect in Rab14- and FAM116-Depleted Cells(A and B) A549 cells were treated with control, Rab14, FAM116A, N-cadherin, E-cadherin, and FGFR2 siRNA duplexes alone or in the combinations indicated for 72 hr (A). The cells were then western blotted for N-cadherin, E-cadherin, or tubulin as a loading control or (B) fixed and then stained with DAPI and antibodies to N-cadherin. Scale bar indicates 10 μm.(C) A549 cells were treated with control, Rab14, FAM116A, N-cadherin, E-cadherin, and FGFR2 siRNA duplexes alone or in the combinations indicated for 72 hr. The cell monolayers were then scratched and imaged for 16 hr. Cell migration was measured and is plotted on the graph with error bars to show the standard error of the mean (n = 3).(D) Images are shown from the 0 and 16 hr time points for the Rab14- and FAM116A-depleted cells in the presence and absence of N-cadherin siRNA. Scale bar indicates 50 μm.See also Figure S5. Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S1534580712001876), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Abnormal Adherens Junctions in Rab14- and FAM116-Depleted Cells(A) A549 cells were treated with control, Rab4, Rab11, Rab14, FAM116A, and Avl9 siRNA duplexes for 72 hr. The cells were lysed in SDS-PAGE sample buffer and then western blotted as indicated in the figure. Tubulin was used as a loading control.(B) A549 cells were treated with control, Rab14, and FAM116A siRNA duplexes for 72 hr, fixed, and then stained with DAPI and antibodies to N-cadherin.(C) A549 cells were treated with control, Rab4, Rab11, Rab14, FAM116A, and Avl9 siRNA duplexes for 72 hr. The cell monolayers were then scratched, samples fixed at 0, 4, 8, and 16 hr, and then stained with DAPI and antibodies to N-cadherin. Images are oriented so the wound is to the right. Scale bar indicates 10 μm in all images.See also Figure S6. Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S1534580712001876), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF6426U-100UG
Alexa Fluor 405	AF6426V-100UG
Alexa Fluor 488	FAB6426G
Alexa Fluor 532	AF6426X-100UG
Alexa Fluor 594	AF6426T-100UG
Alexa Fluor 647	AF6426R-100UG
Alexa Fluor 700	AF6426N-100UG
Alexa Fluor 750	AF6426S-100UG
Alexa Fluor Plus 405	AF6426AFP405-100UG
Alexa Fluor Plus 488	AF6426AFP488-100UG
Alexa Fluor Plus 555	AF6426AFP555-100UG
Alexa Fluor Plus 594	AF6426AFP594-100UG
Alexa Fluor Plus 647	AF6426AFP647-100UG
Alexa Fluor Plus 680	AF6426AFP680-100UG
Allophycocyanin	FAB6426A
Biotin	AF6426B
CoraFluor 1	AF6426CL1
DyLight 350	AF6426D
DyLight 405	AF6426E
DyLight 488	AF6426K
DyLight 550	AF6426L
DyLight 594	AF6426M
DyLight 650	AF6426W
DyLight 680	AF6426Y
DyLight 755	AF6426Z
FITC	AF6426F
HRP	AF6426H
Janelia Fluor 525	AF6426JF525
Janelia Fluor 549	AF6426I
Janelia Fluor 646	AF6426J
Janelia Fluor 669	AF6426JF669
PE	AF6426P
PerCP	AF6426C
mFluor Violet 450 SE	AF6426MFV450
mFluor Violet 500 SE	AF6426MFV500
mFluor Violet 610 SE	AF6426MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB6426A
Carrier Free	FAB6426G
Carrier Free	AF6426-SP

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NBP1-51612

N-Cadherin Antibody (5D5)

(1 Review)

(2 Publications)

0.1 ml

NBP1-51612

Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (5D5) [NBP1-51612] - Immunofluorescence analysis of A431 cells using CDH2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Flow Cytometry: N-Cadherin Antibody (5D5) [NBP1-51612] - Analysis of PC-2 cells using CDH2 mouse mAb (green) and negative control (purple).

Immunohistochemistry-Paraffin: N-Cadherin Antibody (5D5) [NBP1-51612] - Analysis of human Placenta tissues using CDH2 mouse mAb

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-38856

N-Cadherin Antibody - BSA Free

(4 Publications)

0.1 ml

NBP2-38856

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining of human kidney shows strong membranous positivity in cells in tubules.

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining of human skin shows no positivity in keratinocytes.

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-38856] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-38856-25ul

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NBP2-01498

N-Cadherin Antibody (OTI2G7)

(1 Publication)

0.1 ml

NBP2-01498

Flow Cytometry: N Cadherin Antibody (2G7) [NBP2-01498] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-N Cadherin antibody, and then analyzed by flow cytometry.

Western Blot: N Cadherin Antibody (2G7) [NBP2-01498] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY N Cadherin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-N Cadherin.

Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-N Cadherin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-N Cadherin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: N Cadherin Antibody (2G7) [NBP2-01498] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-N Cadherin mouse monoclonal antibody.

Flow Cytometry: N Cadherin Antibody (2G7) [NBP2-01498] - Analysis of Hela cells, using anti-N Cadherin antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Reactivity:

Hu, Mu, Rt, Ca, Pm, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Pm, Pm

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-72891AF350
Alexa Fluor 405	NBP2-72891AF405
Alexa Fluor 488	NBP2-72891AF488
Alexa Fluor 532	NBP2-72891AF532
Alexa Fluor 594	NBP2-72891AF594
Alexa Fluor 647	NBP2-72891AF647
Alexa Fluor 700	NBP2-72891AF700
Alexa Fluor 750	NBP2-72891AF750
Alexa Fluor Plus 405	NBP2-72891AFP405
Alexa Fluor Plus 488	NBP2-72891AFP488
Alexa Fluor Plus 555	NBP2-72891AFP555
Alexa Fluor Plus 594	NBP2-72891AFP594
Alexa Fluor Plus 647	NBP2-72891AFP647
Alexa Fluor Plus 680	NBP2-72891AFP680
Allophycocyanin	NBP2-72891APC
Allophycocyanin/Cy7	NBP2-72891APCCY7
Biotin	NBP2-72891B
CoraFluor 1	NBP2-72891CL1
DyLight 350	NBP2-72891UV
DyLight 405	NBP2-72891V
DyLight 488	NBP2-72891G
DyLight 550	NBP2-72891R
DyLight 594	NBP2-72891DL594
DyLight 650	NBP2-72891C
DyLight 680	NBP2-72891FR
DyLight 755	NBP2-72891IR
FITC	NBP2-72891F
HRP	NBP2-72891H
Janelia Fluor 525	NBP2-72891JF525
Janelia Fluor 549	NBP2-72891JF549
Janelia Fluor 646	NBP2-72891JF646
Janelia Fluor 669	NBP2-72891JF669
PE	NBP2-72891PE
PE/Atto594	NBP2-72891PEATT594
PE/Cy5.5	NBP2-72891PECY55
PE/Cy7	NBP2-72891PECY7
PerCP	NBP2-72891PCP
mFluor Violet 450 SE	NBP2-72891MFV450
mFluor Violet 500 SE	NBP2-72891MFV500
mFluor Violet 610 SE	NBP2-72891MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-72891

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NBP2-19457

N-Cadherin Antibody

(1 Publication)

0.1 ml

NBP2-19457

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (NBP2-19457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

N-Cadherin antibody detects N-Cadherin protein expression by immunohistochemical analysis.<br>Sample: Frozen sectioned E13.5 Rat brain. <br>Green: N-Cadherin protein stained by N-Cadherin antibody (NBP2-19457) diluted at 1:250.<br>Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.<br>Blue: Fluoroshield with DAPI .<br>

<b>Genetic Strategies Validation. </b>Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (NBP2-19457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (NBP2-19457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

N-Cadherin antibody detects N-Cadherin protein at cell membrane by immunofluorescent analysis.<br>Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: N-Cadherin protein stained by N-Cadherin antibody (NBP2-19457) diluted at 1:500.<br>Blue: Hoechst 33342 staining.<br>

Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (NBP2-19457) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

KDM5C/PFDN5 affects EMT in CRC. A protein levels of Vimentin and N-cadherin in HCT116 and SW480 cells determined by WB analysis (two-way ANOVA); B protein levels of Vimentin and N-cadherin in xenograft tumors determined by western blot analysis (two-way ANOVA). For cellular experiments, three biological replicates were performed. For animal studies, n = 5 in each group. *p < 0.05 vs. the sh-NC group; #p < 0.05 vs. the sh-KDM5C group Image collected and cropped by CiteAb from the following open publication (//molmed.biomedcentral.com/articles/10.1186/s10020-023-00775-7), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Bv, Ca, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-21805

N-Cadherin Antibody (GC-4) - Azide and BSA Free

(1 Publication)

0.05 ml

NBP2-21805

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-21805-0.1ml

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NBP2-19459

N-Cadherin Antibody

(1 Publication)

0.1 ml

NBP2-19459

Western Blot: N-Cadherin Antibody [NBP2-19459] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: N-Cadherin Antibody [NBP2-19459] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19459] - Human hepatoma. N-Cadherin antibody [N1N3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19459] - Rat liver. N-Cadherin stained by N-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (NBP2-19459) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

N-Cadherin antibody [N1N3] detects N-Cadherin protein at cell membrane by immunofluorescent analysis.</br>Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: N-Cadherin stained by N-Cadherin antibody [N1N3] (NBP2-19459) diluted at 1:500.</br>Blue: Hoechst 33342 staining.

Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-cadherin antibody (NBP2-19459) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

N-Cadherin antibody [N1N3] detects N-Cadherin protein by western blot analysis.<br />A. 30 ug PC-12 whole cell extract <br />B. 30 ug Rat2 whole cell extract<br />5% SDS-PAGE<br />N-Cadherin antibody [N1N3] (NBP2-19459) dilution: 1:1000 <br />The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP3-15654

N-Cadherin Antibody (6F9G8)

100 ul

NBP3-15654

<b>Genetic Strategies Validation. </b>Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Confocal imaging of paraffin-embedded rat heart using [KO Validated] N-Cadherin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.

Western Blot: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Western blot analysis of lysates from C2C12 cells using [KO Validated] N-Cadherin Rabbit mAb at 1:1000 dilution incubated overnight at 4C.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 60s.

Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Confocal imaging of paraffin-embedded Mouse heart using [KO Validated] N-Cadherin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.<br>

Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Western Blot: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Western blot analysis of lysates from C6 cells using [KO Validated] N-Cadherin Rabbit mAb at 1:1000 dilution incubated overnight at 4C.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 10s.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-19460

N-Cadherin Antibody - BSA Free

0.1 ml

NBP2-19460

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP2-19460] - Paraffin-embedded hepatoma. N-Cadherin antibody [N2C1], Internal dilution: 1:500.

Western Blot: N-Cadherin Antibody [NBP2-19460] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

N-Cadherin antibody [N2C1], Internal detects N-Cadherin protein expression by immunohistochemical analysis.<br>Sample: Frozen sectioned E13.5 Rat brain. <br>Green: N-Cadherin protein stained by N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:250.<br>Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.<br>Blue: Fluoroshield with DAPI .<br>

Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:1000.

Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N2C1], Internal (NBP2-19460) diluted at 1:1000.

Reactivity:

Hu, Mu, Rt, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-61632

N-Cadherin Antibody (CL3716) - BSA Free

100 ul

NBP2-61632

Staining of human skin shows no positivity in epidermal cells as expected.

Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.

Staining of human liver cancer (hepatocellular carcinoma) shows moderate to strong membranous positivity in tumor cells.

Staining of human cerebral cortex shows moderate positivity in neuropil.

Staining of human heart muscle shows strong positivity in the intercalated discs of cardiomyocytes.

Staining of U-251 cells, showing specific staining in the plasma membrane and cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44495AF350
Alexa Fluor 405	NBP3-44495AF405
Alexa Fluor 488	NBP3-44495AF488
Alexa Fluor 532	NBP3-44495AF532
Alexa Fluor 594	NBP3-44495AF594
Alexa Fluor 647	NBP3-44495AF647
Alexa Fluor 700	NBP3-44495AF700
Alexa Fluor 750	NBP3-44495AF750
Alexa Fluor Plus 405	NBP3-44495AFP405
Alexa Fluor Plus 488	NBP3-44495AFP488
Alexa Fluor Plus 555	NBP3-44495AFP555
Alexa Fluor Plus 594	NBP3-44495AFP594
Alexa Fluor Plus 647	NBP3-44495AFP647
Alexa Fluor Plus 680	NBP3-44495AFP680
Allophycocyanin	NBP3-44495APC
Biotin	NBP3-44495B
CoraFluor 1	NBP3-44495CL1
DyLight 350	NBP3-44495UV
DyLight 405	NBP3-44495V
DyLight 488	NBP3-44495G
DyLight 550	NBP3-44495R
DyLight 594	NBP3-44495DL594
DyLight 650	NBP3-44495C
DyLight 680	NBP3-44495FR
DyLight 755	NBP3-44495IR
FITC	NBP3-44495F
HRP	NBP3-44495H
Janelia Fluor 525	NBP3-44495JF525
Janelia Fluor 549	NBP3-44495JF549
Janelia Fluor 646	NBP3-44495JF646
Janelia Fluor 669	NBP3-44495JF669
PE	NBP3-44495PE
PerCP	NBP3-44495PCP
mFluor Violet 450 SE	NBP3-44495MFV450
mFluor Violet 500 SE	NBP3-44495MFV500
mFluor Violet 610 SE	NBP3-44495MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-44495
BSA Free	NBP2-61632-25ul

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NBP3-03009

N-Cadherin Antibody - BSA Free

100 ul

NBP3-03009

Western Blot: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Western blot analysis of lysates from Mouse heart using N-Cadherin Rabbit pAb at 1:1000 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time:30s.

Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Immunofluorescence analysis of paraffin-embedded mouse heart using N-Cadherin Rabbit pAb . Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using N-Cadherin Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using N-Cadherin Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using N-Cadherin Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using N-Cadherin Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-03009-20ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

N-Cadherin Antibodies

N-Cadherin Antibody (13A9) - BSA Free

N-Cadherin Antibody [Unconjugated]

N-Cadherin Antibody (5D5)

N-Cadherin Antibody - BSA Free

N-Cadherin Antibody (OTI2G7)

N-Cadherin Antibody

N-Cadherin Antibody (GC-4) - Azide and BSA Free

N-Cadherin Antibody

N-Cadherin Antibody (6F9G8)

N-Cadherin Antibody - BSA Free

N-Cadherin Antibody (CL3716) - BSA Free

N-Cadherin Antibody - BSA Free