Human MMP-9 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human MMP-9 Quantikine ELISA Kit Summary

• Background: The Quantikine Human MMP-9 Immunoassay is a 3.5 hour solid phase ELISA designed to measure MMP-9 (92 kDa pro- and 82 kDa active forms but not the 65 kDa form) in cell culture supernates, saliva, serum, plasma, and urine. It is calibrated with CHO cell-expressed recombinant human pro-MMP-9 and the antibodies were raised against the recombinant factor. Natural human MMP-9 showed dose-response ...curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative mass values of natural human MMP-9.
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• Specificity: Natural and recombinant human 92 kDa Pro-MMP-9 and the 82 kDa active MMP-9
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: MMP9

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human MMP-9 Quantikine ELISA Kit

• 92 kDa gelatinase
• 92 kDa type IV collagenase
• CLG4B
• EC 3.4.24
• EC 3.4.24.35
• Gelatinase B
• GELB
• macrophage gelatinase
• MANDP2
• matrix metallopeptidase 9
• matrix metalloproteinase 9
• matrix metalloproteinase-9
• MMP9
• MMP-9
• type V collagenase

Background

Matrix metalloproteinases (MMPs), also called matrixins, constitute a family of zinc and calcium dependent endopeptidases that function in the breakdown of the extracellular matrix (ECM) and in the processing of a variety of molecules in different subcellular environments. They play an important role in many normal physiological processes such as embryonic development, morphogenesis, reproduction, and tissue remodeling (1, 2). They also participate in inflammatory and autoimmune disorders such as arthritis, cancer, and cardiovascular disease (3-5). While the amounts of newly synthesized MMPs are regulated mainly at the levels of transcription, the proteolytic activities of existing MMPs are controlled through both the activation of proenzymes or zymogens and the inhibition of active enzymes by endogenous inhibitors, alpha 2-Macroglobulin, and tissue inhibitors of metalloproteinases (TIMPs) (6). 

MMP-9 (also referred to as gelatinase B, 92 kDa type IV collagenase, 92 kDa gelatinase, and type V collagenase) is secreted as a glycosylated proenzyme (6-8). Activation of the proenzyme involves proteolytic removal of the N-terminal pro region, resulting in the 82 kDa active enzyme (9, 10). Active human MMP-9 shares 72% and 74% amino acid sequence identity with mouse and rat MMP-9, respectively. In addition to the zinc-binding site, the catalytic domain also contains three contiguous fibronectin type II homology units responsible for binding gelatin (11). A proline-rich hinge region links the catalytic domain to the C-terminal hemopexin-like domain. In vitro treatment of the proenzyme with 4-aminophenylmercuric acetate (APMA) produces not only the active enzyme but also a C-terminal truncated form with activity comparable to that of the active form (12). MMP-9 degrades components of the ECM with high specific activity for denatured collagens (gelatin). It can cleave native collagens of type III, IV, V, and XI, as well as Elastin, Nidogen-1, and Vitronectin (2, 3). MMP-9 can also cleave a variety of chemokines and growth factors (e.g. IL-1 beta , CXCL8/IL-8, CXCL7, CXCL4, CXCL1, Latent TGF-beta , membrane bound TNF-alpha , VEGF, and FGF basic), Amyloid beta peptide, Substance P, and Myelin Basic Protein (3, 13-15). This action can increase or decrease the biological activity of soluble factors and can also liberate them from association with the ECM (16, 17). MMP-9 can also trigger signaling through various transmembrane proteins or inhibit signaling by inducing their shedding from the cell surface (e.g. CD44, E-Cadherin, Integrins, ICAM-1, and IL-2 R alpha ) (3, 18-20). 

MMP-9 is produced by a variety of normal and transformed cells including neutrophils, monocytes, macrophages, astrocytes, fibroblasts, osteoclasts, chondrocytes, keratinocytes, endothelial and epithelial cells. It exerts physiological and pathological angiogenic and remodeling effects on the vasculature (21-25). Activated neutrophils release proMMP-9 which is free of TIMP-1, allowing the liberation of pro-angiogenic FGF-2 from the ECM (17). MMP-9 in complex with TIMP-1 does not induce FGF-2 release (17). Neutrophil-derived MMP-9 exacerbates the inflammatory response, in part by generating collagen-derived peptides that induce the release of additional neutrophil MMP9 (26). MMP-9 also plays a role in bone formation and remodeling (1, 21, 27), methamphetamineinduced behavioral sensitization and reward (28), the regulation of neuronal synapse remodeling (29), trophoblast invasion during implantation (30), and the inactivation of Serpin alpha 1-Proteinase Inhibitor (31). The shedding of adhesion proteins by MMP-9 has a direct effect on tumor cell invasiveness (18-20). 

Circulating levels of MMP-9 are increased in many inflammatory disorders including intraluminal thrombus formation (32), atherosclerosis (33), Crohn's disease (34), hepatitis C virus infection (35), colorectal cancer (36), and Duchenne muscular dystrophy (37). The ratio of MMP-9 to TIMP-1 is also increased in multiple sclerosis serum (38) and cystic fibrosis sputum (39), but it is decreased in the serum during cytomegalovirus infection (40). Levels of free MMP-9 and complexes of MMP-9 with Lipocalin-2/NGAL are elevated in the urine of ovarian cancer and uterine tract infection patients, respectively (41, 42).

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for MMP-9 (DMP900)(211)

Publications using DMP900

• J Kudelski, A Tokarzewic, M Gudowska-S, B Mroczko, P Ch?osta, M Bruczko-Go, P Mitura, G M?ynarczyk The Significance of Matrix Metalloproteinase 9 (MMP-9) and Metalloproteinase 2 (MMP-2) in Urinary Bladder Cancer Biomedicines, 2023;11(3):. 2023 [PMID: 36979935] (Human)
• LY Wang, CS Tan, MKP Lai, S Hilal Factors Associated with RANTES, EMMPIRIN, MMP2 and MMP9, and the Association of These Biomarkers with Cardiovascular Disease in a Multi-Ethnic Population Journal of Clinical Medicine, 2022;11(24):. 2022 [PMID: 36555898] (Human)
• LTH Nguyen, SH Ahn, HM Shin, IJ Yang Anti-Psoriatic Effect of Rheum palmatum L. and Its Underlying Molecular Mechanisms International Journal of Molecular Sciences, 2022;23(24):. 2022 [PMID: 36555642] (Human)
• I Tsomidis, G Notas, C Xidakis, A Voumvourak, DN Samonakis, M Koulentaki, E Kouroumali Enzymes of Fibrosis in Chronic Liver Disease Biomedicines, 2022;10(12):. 2022 [PMID: 36551935] (Human)
• A Quesnel, N Coles, TM Polvikoski, GS Karagianni, C Angione, M Islam, AA Khundakar, PS Filippou The diagnostic and prognostic potential of the EGFR/MUC4/MMP9 axis in glioma patients Scientific Reports, 2022;12(1):19868. 2022 [PMID: 36400876] (Human)
• A Bahabayi, N Yang, T Xu, Y Xue, L Ma, X Gu, Y Wang, K Jia Expression of Matrix Metalloproteinase-2,-7,-9 in Serum during Pregnancy in Patients with Pre-Eclampsia: A Prospective Study International Journal of Environmental Research and Public Health, 2022;19(21):. 2022 [PMID: 36361378] (Human)
• JV Moxon, AK Kraeuter, J Phie, S Juliano, G Anderson, G Standley, C Sealey, RP White, J Golledge Serum angiopoietin-1 concentration does not distinguish patients with ischaemic stroke from those presenting to hospital with ischaemic stroke mimics BMC cardiovascular disorders, 2022;22(1):462. 2022 [PMID: 36333663] (Human)
• L Zhu, Y Wang, F Qiao microRNA-223 and microRNA-126 are clinical indicators for predicting the plaque stability in carotid atherosclerosis patients Journal of human hypertension, 2022;0(0):. 2022 [PMID: 36192429] (Human)
• GJ Wang, X Dong, Y Li Cluster of Differentiation 147 (CD147) serves as a promoter of atherosclerosis in patients with cerebral infarction European review for medical and pharmacological sciences, 2022;26(16):5710-5717. 2022 [PMID: 36066144] (Human)
• Y Lu, A Yang, C Quan, Y Pan, H Zhang, Y Li, C Gao, H Lu, X Wang, P Cao, H Chen, S Lu, G Zhou A single-cell atlas of the multicellular ecosystem of primary and metastatic hepatocellular carcinoma Nature Communications, 2022;13(1):4594. 2022 [PMID: 35933472] (Human)
• SA Maskarinec, M McKelvy, K Boyle, H Hotchkiss, ME Duarte, B Addison, N Amato, S Khandelwal, GM Arepally, GM Lee Neutrophil functional heterogeneity is a fixed phenotype and is associated with distinct gene expression profiles Journal of leukocyte biology, 2022;0(0):. 2022 [PMID: 35916035] (Human)
• H Ma, JR Albe, T Gilliland, CM McMillen, CL Gardner, DA Boyles, EL Cottle, MD Dunn, JD Lundy, N Salama, KJ O'Malley, I Pandrea, T Teichert, S Barrick, WB Klimstra, AL Hartman, DS Reed Long-term persistence of viral RNA and inflammation in the CNS of macaques exposed to aerosolized Venezuelan equine encephalitis virus Oncogene, 2022;18(6):e1009946. 2022 [PMID: 35696423] (Cynomolgus Macaque)
• M de la Fuen, I Rodríguez-, E Vecino, E Astigarrag, A Acera, G Barreda-Gó Elevation of Tear MMP-9 Concentration as a Biomarker of Inflammation in Ocular Pathology by Antibody Microarray Immunodetection Assays International Journal of Molecular Sciences, 2022;23(10):. 2022 [PMID: 35628448] (Human)
• M Dreiner, T Munk, F Zaucke, AM Liphardt, A Niehoff Relationship between different serum cartilage biomarkers in the acute response to running and jumping in healthy male individuals Scientific Reports, 2022;12(1):6434. 2022 [PMID: 35440750] (Human)
• BG Jung, B Samten, K Dean, RJ Wallace, BA Brown-Elli, T Tucker, S Idell, JV Philley, R Vankayalap Early IL-17A production helps establish Mycobacterium intracellulare infection in mice PloS Pathogens, 2022;18(4):e1010454. 2022 [PMID: 35363832] (Human)
• J Jarecki, T Ma?ecka-Ma, E Kosior-Jar, W Widuchowsk, P Krasowski, M Gutbier, M Dobrzy?ski, T Blicharski Concentration of Selected Metalloproteinases and Osteocalcin in the Serum and Synovial Fluid of Obese Women with Advanced Knee Osteoarthritis International Journal of Environmental Research and Public Health, 2022;19(6):. 2022 [PMID: 35329213] (Human)
• C Xie, B Tang, K Wu, Q Meng, F Wang Increased serum LOXL2 concentration in pelvic inflammatory disease with pelvic adhesion Oncogene, 2022;22(1):59. 2022 [PMID: 35246120] (Human)
• S Okikawa, Y Morine, Y Saito, S Yamada, K Tokuda, H Teraoku, K Miyazaki, S Yamashita, T Ikemoto, S Imura, M Shimada Inhibition of the VEGF signaling pathway attenuates tumor?associated macrophage activity in liver cancer Oncology reports, 2022;47(4):. 2022 [PMID: 35169858] (Human)
• L Raimondi, A Gallo, N Cuscino, A De Luca, V Costa, V Carina, D Bellavia, M Bulati, R Alessandro, M Fini, PG Conaldi, G Giavaresi Potential Anti-Metastatic Role of the Novel miR-CT3 in Tumor Angiogenesis and Osteosarcoma Invasion International Journal of Molecular Sciences, 2022;23(2):. 2022 [PMID: 35054891] (Human)
• MM Ali, I Mirza, D Naquiallah, C Hassan, M Masrur, FM Bianco, AM Mahmoud CD147 Levels in Blood and Adipose Tissues Correlate with Vascular Dysfunction in Obese Diabetic Adults Journal of cardiovascular development and disease, 2021;9(1):. 2021 [PMID: 35050217] (Human)
• C Jiguet-Jig, S Boissonnea, E Denicolai, V Hein, R Lasseur, J Garcia, S Romain, R Appay, T Graillon, W Mason, AF Carpentier, AA Brandes, L' Ouafik, W Wick, A Baaziz, JP Gigan, RJ Argüello, D Figarella-, O Chinot, E Tabouret Plasmatic MMP9 released from tumor-infiltrating neutrophils is predictive for bevacizumab efficacy in glioblastoma patients: an AVAglio ancillary study Acta neuropathologica communications, 2022;10(1):1. 2022 [PMID: 34980260] (Human)
• F Kang, Q Yi, P Gu, Y Dong, Z Zhang, L Zhang, Y Bai Controlled growth factor delivery system with osteogenic-angiogenic coupling effect for bone regeneration Journal of Orthopaedic Translation, 2021;31(0):110-125. 2021 [PMID: 34976731] (Human)
• D Bakkalci, A Jay, A Rezaei, CA Howard, HJ Haugen, J Pape, S Kishida, M Kishida, G Jell, TR Arnett, S Fedele, U Cheema Bioengineering the ameloblastoma tumour to study its effect on bone nodule formation Scientific Reports, 2021;11(1):24088. 2021 [PMID: 34916549] (Human)
• S Nawrocka-M, J Biegus, M Hurkacz, M Guzik, M Rosiek-Bie, EA Jankowska, P Ponikowski, R Zymli?ski Differences in the Biomarker Profile of De Novo Acute Heart Failure versus Decompensation of Chronic Heart Failure Biomolecules, 2021;11(11):. 2021 [PMID: 34827701] (Human)
• A Kuzumi, A Yoshizaki, KM Matsuda, H Kotani, Y Norimatsu, M Fukayama, S Ebata, T Fukasawa, A Yoshizaki-, Y Asano, K Morikawa, Y Kazoe, K Mawatari, T Kitamori, S Sato Interleukin-31 promotes fibrosis and T helper 2 polarization in systemic sclerosis Nature Communications, 2021;12(1):5947. 2021 [PMID: 34642338] (Human)
• K Wadowska, P B?asiak, A Rzechonek, I Bil-Lula, M ?liwi?ska- New Insights on Old Biomarkers Involved in Tumor Microenvironment Changes and Their Diagnostic Relevance in Non-Small Cell Lung Carcinoma Biomolecules, 2021;11(8):. 2021 [PMID: 34439874] (Human)
• L Scola, RM Giarratana, V Marinello, V Cancila, C Pisano, G Ruvolo, G Frati, D Lio, CR Balistreri Polymorphisms of Pro-Inflammatory IL-6 and IL-1&beta Cytokines in Ascending Aortic Aneurysms as Genetic Modifiers and Predictive and Prognostic Biomarkers Biomolecules, 2021;11(7):. 2021 [PMID: 34202072] (Human)
• Y Wu, K Brennan, AB Fernández, MM Mc Gee Cyclophilin A regulates secretion of tumour-derived extracellular vesicles Translational Oncology, 2021;14(8):101112. 2021 [PMID: 33984826] (Human)
• M Jamka, P Bogda?ski, P Krzy?anows, A Mi?kiewicz, J Karolkiewi, M Du?-?uchow, R M?dry, A Lisowska, A Gotz-Wi?ck, S Iskakova, J Walkowiak, E M?dry Endurance Training Depletes Antioxidant System but Does Not Affect Endothelial Functions in Women with Abdominal Obesity: A Randomized Trial with a Comparison to Endurance-Strength Training Journal of Clinical Medicine, 2021;10(8):. 2021 [PMID: 33921520] (Human)
• J Jarecki, T Ma?ecka-Ma, I Polkowska, B Potoczniak, E Kosior-Jar, I Szerb, E Tomaszewsk, M Gutbier, M Dobrzy?ski, T Blicharski Level of Adiponectin, Leptin and Selected Matrix Metalloproteinases in Female Overweight Patients with Primary Gonarthrosis Journal of Clinical Medicine, 2021;10(6):. 2021 [PMID: 33803785] (Human)
• YJ Shin, H Vu, JH Lee, HD Kim Diagnostic and prognostic ability of salivary MMP-9 for oral squamous cell carcinoma: A pre-/post-surgery case and matched control study PLoS ONE, 2021;16(3):e0248167. 2021 [PMID: 33735248] (Human)
• S Sriphoosan, K Thanapirom, SJ Kerr, S Suksawatam, P Thaimai, S Sittisomwo, K Sonsiri, N Srisoontho, N Teeratorn, N Tanpowpong, B Chaopathom, S Treepraser, Y Poovorawan, P Komolmit Effect of vitamin D supplementation in patients with chronic hepatitis C after direct-acting antiviral treatment: a randomized, double-blind, placebo-controlled trial PeerJ, 2021;9(0):e10709. 2021 [PMID: 33614272] (Human)
• C Guo, L Sun, L Zhang, F Dong, X Zhang, L Yao, C Chang Serum sphingolipid profile in asthma Journal of leukocyte biology, 2021;0(0):. 2021 [PMID: 33600023] (Human)
• JR Albe, H Ma, TH Gilliland, CM McMillen, CL Gardner, DA Boyles, EL Cottle, MD Dunn, JD Lundy, KJ O'Malley, N Salama, AW Walters, I Pandrea, T Teichert, WB Klimstra, DS Reed, AL Hartman Physiological and immunological changes in the brain associated with lethal eastern equine encephalitis virus in macaques PloS Pathogens, 2021;17(2):e1009308. 2021 [PMID: 33534855] (Primate)
• X Li, F Zhu, Z Liu, X Tang, Y Han, J Jiang, C Ma, Y He High expression of Rab31 confers a poor prognosis and enhances cell proliferation and invasion in oral squamous cell carcinoma Oncology reports, 2021;0(0):. 2021 [PMID: 33469675] (Human)
• VG Arcos-Sacr, CL Sampieri, VH Sandoval-L, RA Orozco-Ort, MA Acuña-Hern, J Morales-Ro, ME Hernández-, A Rodríguez- Urinary MMP-9/UCr association with albumin concentration and albumin-creatinine-ratio in Mexican patients with type 2 diabetes mellitus PeerJ, 2020;8(0):e10474. 2020 [PMID: 33362965] (Human)
• J Jandova, GT Wondrak Genomic GLO1 deletion modulates TXNIP expression, glucose metabolism, and redox homeostasis while accelerating human A375 malignant melanoma tumor growth Redox Biology, 2020;39(0):101838. 2020 [PMID: 33360689] (Human)
• A Waszczykow, M Podgórski, M Waszczykow, Z Gerlicz-Ko, P Jurowski Matrix Metalloproteinases MMP-2 and MMP-9, Their Inhibitors TIMP-1 and TIMP-2, Vascular Endothelial Growth Factor and sVEGFR-2 as Predictive Markers of Ischemic Retinopathy in Patients with Systemic Sclerosis-Case Series Report Int J Mol Sci, 2020;21(22):. 2020 [PMID: 33218057] (Human)
• H Kook, DH Jang, JH Kim, JY Cho, HJ Joo, SA Cho, JH Park, SJ Hong, CW Yu, DS Lim Identification of plaque ruptures using a novel discriminative model comprising biomarkers in patients with acute coronary syndrome Sci Rep, 2020;10(1):20228. 2020 [PMID: 33214686] (Human)
• MMS Balla, S Patwardhan, PK Melwani, P Purwar, A Kumar, CS Pramesh, S Laskar, BN Pandey Prognosis of metastasis based on age and serum analytes after follow-up of non-metastatic lung cancer patients Transl Oncol, 2020;14(1):100933. 2020 [PMID: 33188981] (Human)
• T Phillips, L Huitema, R Cepeda, DL Cobos, RIM Perez, MS Garza, F Ringpfeil, B Dasgeb, J Uitto, JC Salas-Alan, V Alexeev, O Igoucheva Aberrant recruitment of leukocytes defines poor wound healing in patients with recessive dystrophic epidermolysis bullosa J Dermatol Sci, 2020;0(0):. 2020 [PMID: 33143962] (Human)
• JJ Noh, MS Kim, YJ Cho, SY Jeong, YY Lee, JY Ryu, JJ Choi, I Bae, Z Wu, BG Kim, JR Hwang, JW Lee Anti-Cancer Activity of As4O6 and its Efficacy in a Series of Patient-Derived Xenografts for Human Cervical Cancer Pharmaceutics, 2020;12(10):. 2020 [PMID: 33086573] (Human)
• L Raimondi, A De Luca, S Fontana, N Amodio, V Costa, V Carina, D Bellavia, S Raimondo, S Siragusa, F Monteleone, R Alessandro, M Fini, G Giavaresi Multiple Myeloma-Derived Extracellular Vesicles Induce Osteoclastogenesis through the Activation of the XBP1/IRE1alpha Axis Cancers (Basel), 2020;12(8):. 2020 [PMID: 32759820] (Human)
• L Raimondi, A De Luca, S Fontana, N Amodio, V Costa, V Carina, D Bellavia, S Raimondo, S Siragusa, F Monteleone, R Alessandro, M Fini, G Giavaresi Multiple Myeloma-Derived Extracellular Vesicles Induce Osteoclastogenesis through the Activation of the XBP1/IRE1&alpha Axis Cancers (Basel), 2020;12(8):. 2020 [PMID: 32759820] (Human)
• J Jandova, J Perer, A Hua, JA Snell, GT Wondrak Genetic Target Modulation Employing CRISPR/Cas9 Identifies Glyoxalase 1 as a Novel Molecular Determinant of Invasion and Metastasis in A375 Human Malignant Melanoma Cells In Vitro and In Vivo Cancers (Basel), 2020;12(6):. 2020 [PMID: 32466621] (Human)
• S Hayat, O Ahmad, I Mahmud, MZH Howlader, Z Islam Association of matrix metalloproteinase-9 polymorphism with severity of Guillain-Barr� syndrome J. Neurol. Sci., 2020;415(0):116908. 2020 [PMID: 32447054] (Human)
• AE Stanciu, A Zamfir-Chi, MM Stanciu, A Pantea-Sto, C Nitipir, DC Gheorghe Serum melatonin is inversely associated with matrix metalloproteinase-9 in oral squamous cell carcinoma Oncol Lett, 2020;19(4):3011-3020. 2020 [PMID: 32218858] (Human)
• YY Liu, SY Wang, YN Li, WJ Bian, LQ Zhang, YH Li, L Long, X Liu, XW Zhang, ZG Li Activity of fibroblast-like synoviocytes in rheumatoid arthritis was impaired by dickkopf-1 targeting siRNA Chin. Med. J., 2020;0(6):679-686. 2020 [PMID: 32068606] (Human)
• CS Kuo, JS Chen, LY Lin, GW Schmid-Sch, S Chien, PH Huang, JW Chen, SJ Lin Inhibition of Serine Protease Activity Protects Against High Fat Diet-Induced Inflammation and Insulin Resistance Sci Rep, 2020;10(1):1725. 2020 [PMID: 32015418] (Human)
• Z Wang, D Ye, J Ye, M Wang, J Liu, H Jiang, Y Xu, J Zhang, J Chen, J Wan ADAMTS-5 Decreases in Coronary Arteries and Plasma from Patients with Coronary Artery Disease Dis. Markers, 2019;2019(0):6129748. 2019 [PMID: 31929842] (Human)
• M Yamauchi, T Kinjo, G Parrott, K Miyagi, S Haranaga, Y Nakayama, K Chibana, K Fujita, A Nakamoto, F Higa, I Owan, K Yonemoto, J Fujita Diagnostic performance of serum interferon gamma, matrix metalloproteinases, and periostin measurements for pulmonary tuberculosis in Japanese patients with pneumonia PLoS ONE, 2020;15(1):e0227636. 2020 [PMID: 31917802] (Human)
• A Kobus, J Bagi?ska, J ?api?ska-A, S ?awicki, A Kierklo Levels of Selected Matrix Metalloproteinases, Their Inhibitors in Saliva, and Oral Status in Juvenile Idiopathic Arthritis Patients vs. Healthy Controls Biomed Res Int, 2019;2019(0):7420345. 2019 [PMID: 31781639] (Human)
• HL Lung, ML Lung, S Law Serum amyloid A1 polymorphisms as risk factors in oesophageal squamous cell carcinoma Hong Kong Med J, 2019;25(5):4-8. 2019 [PMID: 31761762] (Human)
• DP Zhang, YF Peng, HL Zhang, JG Ma, M Zhao, S Yin, TT Wei Basilar Artery Tortuosity Is Associated With White Matter Hyperintensities by TIMP-1 Front Neurosci, 2019;13(0):836. 2019 [PMID: 31474817] (Human)
• N Sabbah, S Jaisson, R Garnotel, E Anglés-Can, P Gillery Small size apolipoprotein(a) isoforms enhance inflammatory and proteolytic potential of collagen-primed monocytes Lipids Health Dis, 2019;18(1):166. 2019 [PMID: 31470857] (Human)
• B Singh, N Ghosh, D Saha, S Sarkar, P Bhattachar, K Chaudhury Effect of doxycyline in chronic obstructive pulmonary disease - An exploratory study Pulm Pharmacol Ther, 2019;0(0):101831. 2019 [PMID: 31349003] (Human)
• B Singh, N Ghosh, D Saha, S Sarkar, P Bhattachar, K Chaudhury Effect of doxycyline in chronic obstructive pulmonary disease - An exploratory study Pulm Pharmacol Ther, 2019;0(0):101831. 2019 [PMID: 31349003] (Human)
• L Lorente, MM Martín, L Ramos, M Argueso, JJ Cáceres, J Solé-Violá, A Jiménez, JM Borreguero, AF González-R, J Orbe, JA Rodríguez, JA Páramo High serum levels of tissue inhibitor of matrix metalloproteinase-1 during the first week of a malignant middle cerebral artery infarction in non-surviving patients BMC Neurol, 2019;19(1):167. 2019 [PMID: 31319804] (Human)
• L Lorente, MM Martín, L Ramos, M Argueso, JJ Cáceres, J Solé-Violá, A Jiménez, JM Borreguero, AF González-R, J Orbe, JA Rodríguez, JA Páramo High serum levels of tissue inhibitor of matrix metalloproteinase-1 during the first week of a malignant middle cerebral artery infarction in non-surviving patients BMC Neurol, 2019;19(1):167. 2019 [PMID: 31319804] (Human)
• D Štrbac, K Gori?ar, V Dolžan, V Kova? Evaluation of Matrix Metalloproteinase 9 Serum Concentration as a Biomarker in Malignant Mesothelioma Dis. Markers, 2019;2019(0):1242964. 2019 [PMID: 31191742] (Human)
• M Matulka, A Konopka, B Mroczko, A Pryczynicz, A Kemona, M Groblewska, A Sieskiewic, E Olszewska Expression and Concentration of Matrix Metalloproteinase 9 and Tissue Inhibitor of Matrix Metalloproteinases 1 in Laryngeal Squamous Cell Carcinoma Dis. Markers, 2019;2019(0):3136792. 2019 [PMID: 31143300] (Human)
• L Lorente, MM Martín, L Ramos, M Argueso, JJ Cáceres, J Solé-Violá, A Jiménez, JM Borreguero, AF González-R, J Orbe, JA Rodríguez, JA Páramo Persistently high circulating tissue inhibitor of matrix metalloproteinase-1 levels in non-survivor brain trauma injury patients J Crit Care, 2019;51(0):117-121. 2019 [PMID: 30802757] (Human)
• A Darlix, C Hirtz, S Thezenas, A Maceski, A Gabelle, E Lopez-Crap, H De Forges, N Firmin, S Guiu, W Jacot, S Lehmann The prognostic value of the�Tau protein serum level in metastatic breast cancer patients and its correlation with brain metastases BMC Cancer, 2019;19(1):110. 2019 [PMID: 30700265] (Human)
• H Yogasundar, A Nikhanj, BN Putko, M Boutin, S Jain-Ghai, A Khan, C Auray-Blai, ML West, GY Oudit Elevated Inflammatory Plasma Biomarkers in Patients With Fabry Disease: A Critical Link to Heart Failure With Preserved Ejection Fraction J Am Heart Assoc, 2018;7(21):e009098. 2018 [PMID: 30571380] (Human)
• AU García-Tej, CL Sampieri, I Suárez-Tor, J Morales-Ro, VP Demeneghi-, ME Hernández-, A Rodríguez- Association of urinary activity of MMP-9 with renal impairment in Mexican patients with type 2 diabetes mellitus PeerJ, 2018;6(0):e6067. 2018 [PMID: 30564523] (Human)
• ZJ Shen, CS Xu, YP Li, J Li, JJ Xu, P Xia Telmisartan inhibits Ang II-induced MMP-9 expression in macrophages in stabilizing atheromatous plaque Eur Rev Med Pharmacol Sci, 2018;22(22):8004-8012. 2018 [PMID: 30536349] (Human)
• DC Woodworth, A Dagher, A Curatolo, M Sachdev, C Ashe-McNal, BD Naliboff, JS Labus, JR Landis, JJ Kutch, EA Mayer, RS Lee, MA Moses, BM Ellingson, Changes in brain white matter structure are associated with urine proteins in urologic chronic pelvic pain syndrome (UCPPS): A MAPP Network study PLoS ONE, 2018;13(12):e0206807. 2018 [PMID: 30517112] (Human)
• CK Wu, MM Su, YF Wu, JJ Hwang, LY Lin Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with Preserved Ejection Fraction Patients J Clin Med, 2018;7(11):. 2018 [PMID: 30413105] (Human)
• S Zeng, B Zhu, J Zeng, W Wu, C Jiang Zeylenone represses the progress of human prostate cancer by downregulating the Wnt/??catenin pathway Mol Med Rep, 2018;0(0):. 2018 [PMID: 30365080] (Human)
• Y Li, L Chen, S Yao, J Chen, W Hu, M Wang, S Chen, X Chen, S Li, X Gu, G Ma, B Zhao, W Zhong, K Li Association of Polymorphisms of the Matrix Metalloproteinase 9 Gene with Ischaemic Stroke in a Southern Chinese Population Cell. Physiol. Biochem., 2018;49(6):2188-2199. 2018 [PMID: 30257242] (Human)
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• Agarwal A, Covic L, Sevigny LM, Kaneider NC, Lazarides K, Azabdaftari G, Sharifi S, Kuliopulos A Targeting a metalloprotease-PAR1 signaling system with cell-penetrating pepducins inhibits angiogenesis, ascites, and progression of ovarian cancer. Mol. Cancer Ther., 2008;7(9):2746-57. 2008 [PMID: 18790755] (Human)
• Samnegard A, Hulthe J, Silveira A, Ericsson CG, Hamsten A, Eriksson P Gender specific associations between matrix metalloproteinases and inflammatory markers in post myocardial infarction patients. Atherosclerosis, 2009;202(2):550-6. 2009 [PMID: 18619594] (Human)
• Coral K, Angayarkanni N, Madhavan J, Bharathselvi M, Ramakrishnan S, Nandi K, Rishi P, Kasinathan N, Krishnakumar S Lysyl oxidase activity in the ocular tissues and the role of LOX in proliferative diabetic retinopathy and rhegmatogenous retinal detachment. Invest. Ophthalmol. Vis. Sci., 2008;49(11):4746-52. 2008 [PMID: 18566459] (Human)
• Cowden Dahl KD, Symowicz J, Ning Y, Gutierrez E, Fishman DA, Adley BP, Stack MS, Hudson LG Matrix metalloproteinase 9 is a mediator of epidermal growth factor-dependent e-cadherin loss in ovarian carcinoma cells. Cancer Res., 2008;68(12):4606-13. 2008 [PMID: 18559505] (Human)
• Bendrik C, Robertson J, Gauldie J, Dabrosin C Gene transfer of matrix metalloproteinase-9 induces tumor regression of breast cancer in vivo. Cancer Res., 2008;68(9):3405-12. 2008 [PMID: 18451168] (Human)
• Glader P, Eldh B, Bozinovski S, Andelid K, Sjostrand M, Malmhall C, Anderson GP, Riise GC, Qvarfordt I, Linden A Impact of acute exposure to tobacco smoke on gelatinases in the bronchoalveolar space. Eur. Respir. J., 2008;32(3):644-50. 2008 [PMID: 18417512] (Human)
• Smith ER, Zurakowski D, Saad A, Scott RM, Moses MA Urinary biomarkers predict brain tumor presence and response to therapy. Clin. Cancer Res., 2008;14(8):2378-86. 2008 [PMID: 18413828] (Human)
• Huh JW, Kim DS, Oh YM, Shim TS, Lim CM, Lee SD, Koh Y, Kim WS, Kim WD, Kim KR Is metalloproteinase-7 specific for idiopathic pulmonary fibrosis? Chest, 2007;133(5):1101-6. 2007 [PMID: 18071010] (Human)
• Han WK, Waikar SS, Johnson A, Betensky RA, Dent CL, Devarajan P, Bonventre JV Urinary biomarkers in the early diagnosis of acute kidney injury. Kidney Int., 2007;73(7):863-9. 2007 [PMID: 18059454] (Human)
• Simpson JL, Powell H, Boyle MJ, Scott RJ, Gibson PG Clarithromycin targets neutrophilic airway inflammation in refractory asthma. Am. J. Respir. Crit. Care Med., 2007;177(2):148-55. 2007 [PMID: 17947611] (Human)
• Goleva E, Hauk PJ, Boguniewicz J, Martin RJ, Leung DY Airway remodeling and lack of bronchodilator response in steroid-resistant asthma. J. Allergy Clin. Immunol., 2007;120(5):1065-72. 2007 [PMID: 17900681] (Human)
• Vishnubhotla R, Sun S, Huq J, Bulic M, Ramesh A, Guzman G, Cho M, Glover SC ROCK-II mediates colon cancer invasion via regulation of MMP-2 and MMP-13 at the site of invadopodia as revealed by multiphoton imaging. Lab. Invest., 2007;87(11):1149-58. 2007 [PMID: 17876296] (Human)
• Kleinman ME, Greives MR, Churgin SS, Blechman KM, Chang EI, Ceradini DJ, Tepper OM, Gurtner GC Hypoxia-induced mediators of stem/progenitor cell trafficking are increased in children with hemangioma. Arterioscler. Thromb. Vasc. Biol., 2007;27(12):2664-70. 2007 [PMID: 17872454] (Human)
• Forst T, Karagiannis E, Lubben G, Hohberg C, Schondorf T, Dikta G, Drexler M, Morcos M, Danschel W, Borchert M, Pfutzner A Pleiotrophic and anti-inflammatory effects of pioglitazone precede the metabolic activity in type 2 diabetic patients with coronary artery disease. Atherosclerosis, 2007;197(1):311-7. 2007 [PMID: 17588584] (Human)
• Kissel CK, Lehmann R, Assmus B, Aicher A, Honold J, Fischer-Rasokat U, Heeschen C, Spyridopoulos I, Dimmeler S, Zeiher AM Selective functional exhaustion of hematopoietic progenitor cells in the bone marrow of patients with postinfarction heart failure. J. Am. Coll. Cardiol., 2007;49(24):2341-9. 2007 [PMID: 17572250] (Human)
• Nabata A, Kuroki M, Ueba H, Hashimoto S, Umemoto T, Wada H, Yasu T, Saito M, Momomura S, Kawakami M C-reactive protein induces endothelial cell apoptosis and matrix metalloproteinase-9 production in human mononuclear cells: Implications for the destabilization of atherosclerotic plaque. Atherosclerosis, 2007;196(1):129-35. 2007 [PMID: 17531242] (Human)
• Zinner RG, Nemunaitis J, Eiseman I, Shin HJ, Olson SC, Christensen J, Huang X, Lenehan PF, Donato NJ, Shin DM Phase I clinical and pharmacodynamic evaluation of oral CI-1033 in patients with refractory cancer. Clin. Cancer Res., 2007;13(10):3006-14. 2007 [PMID: 17505003] (Human)
• Zhao Z, McCloud B, Fleming R, Klempner MS Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro. Biochem. Biophys. Res. Commun., 2007;358(2):528-33. 2007 [PMID: 17485071] (Human)
• Wu CY, Wu MS, Chiang EP, Chen YJ, Chen CJ, Chi NH, Shih YT, Chen GH, Lin JT Plasma matrix metalloproteinase-9 level is better than serum matrix metalloproteinase-9 level to predict gastric cancer evolution. Clin. Cancer Res., 2007;13(7):2054-60. 2007 [PMID: 17404086] (Human)
• Gaggar A, Li Y, Weathington N, Winkler M, Kong M, Jackson P, Blalock JE, Clancy JP Matrix metalloprotease-9 dysregulation in lower airway secretions of cystic fibrosis patients. Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L96-L104. 2007 [PMID: 17384080] (Human)
• Apple FS, Pearce LA, Chung A, Ler R, Murakami MM Multiple biomarker use for detection of adverse events in patients presenting with symptoms suggestive of acute coronary syndrome. Clin. Chem., 2007;53(5):874-81. 2007 [PMID: 17384009] (Human)
• Bodet C, Andrian E, Tanabe S, Grenier D Actinobacillus actinomycetemcomitans lipopolysaccharide regulates matrix metalloproteinase, tissue inhibitors of matrix metalloproteinase, and plasminogen activator production by human gingival fibroblasts: a potential role in connective tissue destruction. J. Cell. Physiol., 2007;212(1):189-94. 2007 [PMID: 17299802] (Human)
• Ma DH, Yao JY, Kuo MT, See LC, Lin KY, Chen SC, Chen JK, Chao AS, Wang SF, Lin KK Generation of endostatin by matrix metalloproteinase and cathepsin from human limbocorneal epithelial cells cultivated on amniotic membrane. Invest. Ophthalmol. Vis. Sci., 2007;48(2):644-51. 2007 [PMID: 17251461] (Human)
• Yee LD, Williams N, Wen P, Young DC, Lester J, Johnson MV, Farrar WB, Walker MJ, Povoski SP, Suster S, Eng C Pilot study of rosiglitazone therapy in women with breast cancer: effects of short-term therapy on tumor tissue and serum markers. Clin. Cancer Res., 2007;13(1):246-52. 2007 [PMID: 17200362] (Human)
• Sherman JA, Hall A, Malenka DJ, De Muinck ED, Simons M Humoral and cellular factors responsible for coronary collateral formation. Am. J. Cardiol., 2006;98(9):1194-7. 2006 [PMID: 17056326] (Human)
• Johannsen A, Rylander G, Soder B, Asberg M Dental plaque, gingival inflammation, and elevated levels of interleukin-6 and cortisol in gingival crevicular fluid from women with stress-related depression and exhaustion. J. Periodontol., 2006;77(8):1403-9. 2006 [PMID: 16937592] (Human)
• Borrelli V, di Marzo L, Sapienza P, Colasanti M, Moroni E, Cavallaro A Role of platelet-derived growth factor and transforming growth factor beta1 the in the regulation of metalloproteinase expressions. Surgery, 2006;140(3):454-63. 2006 [PMID: 16934609] (Human)
• Molica S, Vitelli G, Mirabelli R, Digiesu G, Giannarelli D, Cuneo A, Ribatti D, Vacca A Serum levels of syndecan-1 in B-cell chronic lymphocytic leukemia: correlation with the extent of angiogenesis and disease-progression risk in early disease. Leuk. Lymphoma, 2006;47(6):1034-40. 2006 [PMID: 16840194] (Human)
• Rofstad EK, Mathiesen B, Kindem K, Galappathi K Acidic extracellular pH promotes experimental metastasis of human melanoma cells in athymic nude mice. Cancer Res., 2006;66(13):6699-707. 2006 [PMID: 16818644] (Human)
• Lee KS, Min KH, Kim SR, Park SJ, Park HS, Jin GY, Lee YC Vascular endothelial growth factor modulates matrix metalloproteinase-9 expression in asthma. Am. J. Respir. Crit. Care Med., 2006;174(2):161-70. 2006 [PMID: 16645174] (Human)
• Merck E, Gaillard C, Scuiller M, Scapini P, Cassatella MA, Trinchieri G, Bates EE Ligation of the FcR gamma chain-associated human osteoclast-associated receptor enhances the proinflammatory responses of human monocytes and neutrophils. J. Immunol., 2006;176(5):3149-56. 2006 [PMID: 16493074] (Human)
• Chu CS, Lee KT, Lee MY, Su HM, Voon WC, Sheu SH, Lai WT Effects of rosiglitazone alone and in combination with atorvastatin on nontraditional markers of cardiovascular disease in patients with type 2 diabetes mellitus. Am. J. Cardiol., 2006;97(5):646-50. 2006 [PMID: 16490430] (Human)
• Adelroth E, Hedlund U, Blomberg A, Helleday R, Ledin MC, Levin JO, Pourazar J, Sandstrom T, Jarvholm B Airway inflammation in iron ore miners exposed to dust and diesel exhaust. Eur. Respir. J., 2006;27(4):714-9. 2006 [PMID: 16455836] (Human)
• Niessner A, Richter B, Penka M, Steiner S, Strasser B, Ziegler S, Heeb-Elze E, Zorn G, Leitner-Heinschink A, Niessner C, Wojta J, Huber K Endurance training reduces circulating inflammatory markers in persons at risk of coronary events: impact on plaque stabilization? Atherosclerosis, 2005;186(1):160-5. 2005 [PMID: 16084517] (Human)
• Figueredo CM, Fischer RG, Gustafsson A Aberrant neutrophil reactions in periodontitis. J. Periodontol., 2005;76(6):951-5. 2005 [PMID: 15948690] (Human)
• Ko FW, Diba C, Roth M, McKay K, Johnson PR, Salome C, King GG A comparison of airway and serum matrix metalloproteinase-9 activity among normal subjects, asthmatic patients, and patients with asthmatic mucus hypersecretion. Chest, 2005;127(6):1919-27. 2005 [PMID: 15947303] (Human)
• Simpson JL, Scott RJ, Boyle MJ, Gibson PG Differential proteolytic enzyme activity in eosinophilic and neutrophilic asthma. Am. J. Respir. Crit. Care Med., 2005;172(5):559-65. 2005 [PMID: 15901607] (Human)
• Rouy D, Ernens I, Jeanty C, Wagner DR Plasma storage at -80 degrees C does not protect matrix metalloproteinase-9 from degradation. Anal. Biochem., 2005;338(2):294-8. 2005 [PMID: 15745750] (Human)
• Sagel SD, Kapsner RK, Osberg I Induced sputum matrix metalloproteinase-9 correlates with lung function and airway inflammation in children with cystic fibrosis. Pediatr. Pulmonol., 2005;39(3):224-32. 2005 [PMID: 15635615] (Human)
• Watelet JB, Demetter P, Claeys C, Van Cauwenberge P, Cuvelier C, Bachert C Neutrophil-derived metalloproteinase-9 predicts healing quality after sinus surgery. Laryngoscope, 2005;115(1):56-61. 2005 [PMID: 15630367] (Human)
• Li DQ, Chen Z, Song XJ, Luo L, Pflugfelder SC Stimulation of matrix metalloproteinases by hyperosmolarity via a JNK pathway in human corneal epithelial cells. Invest. Ophthalmol. Vis. Sci., 2004;45(12):4302-11. 2004 [PMID: 15557436] (Human)
• Singh S, Singh UP, Grizzle WE, Lillard JW CXCL12-CXCR4 interactions modulate prostate cancer cell migration, metalloproteinase expression and invasion. Lab. Invest., 2004;84(12):1666-76. 2004 [PMID: 15467730] (Human)
• D'Ascenzo S, Giusti I, Millimaggi D, Marci R, Tatone C, Cardigno Colonna R, Moscarini M, Pavan A, Dolo V, Caserta D Intrafollicular expression of matrix metalloproteinases and their inhibitors in normally ovulating women compared with patients undergoing in vitro fertilization treatment. Eur. J. Endocrinol., 2004;151(1):87-91. 2004 [PMID: 15248826] (Human)
• Jumper C, Cobos E, Lox C Determination of the serum matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in patients with either advanced small-cell lung cancer or non-small-cell lung cancer prior to treatment. Respir Med, 2004;98(2):173-7. 2004 [PMID: 14971882] (Human)
• Laudanski P, Reduta T Matrix metalloprotenase-9 in acute pyelonephritis. Pediatr. Res., 2004;55(2):357; author r. 2004 [PMID: 14763447] (Human)
• Doubrovina ES, Doubrovin MM, Vider E, Sisson RB, O'Reilly RJ, Dupont B, Vyas YM Evasion from NK cell immunity by MHC class I chain-related molecules expressing colon adenocarcinoma. J. Immunol., 2003;171(12):6891-9. 2003 [PMID: 14662896] (Human)
• Tziakas DN, Chalikias GK, Hatzinikolaou HI, Parissis JT, Papadopoulos ED, Trypsianis GA, Papadopoulou E, Tentes IK, Karas SM, Hatseras DI Anti-inflammatory cytokine profile in acute coronary syndromes: behavior of interleukin-10 in association with serum metalloproteinases and proinflammatory cytokines. Int. J. Cardiol., 2003;92(2):169-75. 2003 [PMID: 14659849] (Human)
• Ulrich D, Hrynyschyn K, Pallua N Matrix metalloproteinases and tissue inhibitors of metalloproteinases in sera and tissue of patients with Dupuytren's disease. Plast. Reconstr. Surg., 2003;112(5):1279-86. 2003 [PMID: 14504511] (Human)
• Young KA, Stouffer RL Gonadotropin and steroid regulation of matrix metalloproteinases and their endogenous tissue inhibitors in the developed corpus luteum of the rhesus monkey during the menstrual cycle. Biol. Reprod., 2003;70(1):244-52. 2003 [PMID: 13679308] (Primate)
• D'Agostino P Matrix metalloproteinases production in malignant pleural effusions after talc pleurodesis. Clin. Exp. Immunol., 2003;134(1):138-42. 2003 [PMID: 12974766] (Human)
• Marx N, Froehlich J, Siam L, Ittner J, Wierse G, Schmidt A, Scharnagl H, Hombach V, Koenig W Antidiabetic PPAR gamma-activator rosiglitazone reduces MMP-9 serum levels in type 2 diabetic patients with coronary artery disease. Arterioscler. Thromb. Vasc. Biol., 2003;23(2):283-8. 2003 [PMID: 12588772] (Human)
• Cataldo DD, Bettiol J, Noel A, Bartsch P, Foidart JM, Louis R Matrix metalloproteinase-9, but not tissue inhibitor of matrix metalloproteinase-1, increases in the sputum from allergic asthmatic patients after allergen challenge. Chest, 2002;122(5):1553-9. 2002 [PMID: 12426252] (Human)
• Fireman E, Kraiem Z, Sade O, Greif J, Fireman Z Induced sputum-retrieved matrix metalloproteinase 9 and tissue metalloproteinase inhibitor 1 in granulomatous diseases. Clin. Exp. Immunol., 2002;130(2):331-7. 2002 [PMID: 12390324] (Human)
• Szamatowicz J, Laudanski P, Tomaszewska I Matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1: a possible role in the pathogenesis of endometriosis. Hum. Reprod., 2002;17(2):284-8. 2002 [PMID: 11821264] (Human)

FAQs for MMP-9 (DMP900). (Showing 1 - 1 of 1 FAQs).

1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
   • We have 10 primary antibodies for MMP-9 that have been tested in ELISA.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&applications=ELISA&common_name=MMP-9It looks like 2 have been tested for capture (please note the tested species for each of these).https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Capture%20(Matched%20Antibody%20Pair)1 has been tested for detection.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Detection%20(Matched%20Antibody%20Pair)

Bioinformatics

• Gene Symbol: MMP9