Human MMP-9 Quantikine ELISA Kit

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Catalog# & Conjugate Size Price

Human MMP-9 Quantikine ELISA Kit Summary

Background
The Quantikine Human MMP-9 Immunoassay is a 3.5 hour solid phase ELISA designed to measure MMP-9 (92 kDa pro- and 82 kDa active forms but not the 65 kDa form) in cell culture supernates, saliva, serum, plasma, and urine. It is calibrated with CHO cell-expressed recombinant human pro-MMP-9 and the antibodies were raised against the recombinant factor. Natural human MMP-9 showed dose-response ...curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative mass values of natural human MMP-9.
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Specificity
Natural and recombinant human 92 kDa Pro-MMP-9 and the 82 kDa active MMP-9
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
MMP9

Applications/Dilutions

Application Notes
Interference observed with 1 or more available related molecules.
Publications
Read Publications using
DMP900 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human MMP-9 Quantikine ELISA Kit

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Background

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for MMP-9 (DMP900)(161)

We have publications tested in 3 confirmed species: Human, Mouse, Primate.

We have publications tested in 1 application: ELISA Capture.


Filter By Application
ELISA Capture
(3)
All Applications
Filter By Species
Human
(159)
Mouse
(1)
Primate
(1)
All Species
Showing Publications 1 - 10 of 161. Show All 161 Publications.
Publications using DMP900 Applications Species
Z Wang, D Ye, J Ye, M Wang, J Liu, H Jiang, Y Xu, J Zhang, J Chen, J Wan ADAMTS-5 Decreases in Coronary Arteries and Plasma from Patients with Coronary Artery Disease Dis. Markers, 2019;2019(0):6129748. 2019 [PMID: 31929842] (Human) Human
M Yamauchi, T Kinjo, G Parrott, K Miyagi, S Haranaga, Y Nakayama, K Chibana, K Fujita, A Nakamoto, F Higa, I Owan, K Yonemoto, J Fujita Diagnostic performance of serum interferon gamma, matrix metalloproteinases, and periostin measurements for pulmonary tuberculosis in Japanese patients with pneumonia PLoS ONE, 2020;15(1):e0227636. 2020 [PMID: 31917802] (Human) Human
A Kobus, J Bagi?ska, J ?api?ska-A, S ?awicki, A Kierklo Levels of Selected Matrix Metalloproteinases, Their Inhibitors in Saliva, and Oral Status in Juvenile Idiopathic Arthritis Patients vs. Healthy Controls Biomed Res Int, 2019;2019(0):7420345. 2019 [PMID: 31781639] (Human) Human
HL Lung, ML Lung, S Law Serum amyloid A1 polymorphisms as risk factors in oesophageal squamous cell carcinoma Hong Kong Med J, 2019;25(5):4-8. 2019 [PMID: 31761762] (Human) Human
DP Zhang, YF Peng, HL Zhang, JG Ma, M Zhao, S Yin, TT Wei Basilar Artery Tortuosity Is Associated With White Matter Hyperintensities by TIMP-1 Front Neurosci, 2019;13(0):836. 2019 [PMID: 31474817] (Human) Human
N Sabbah, S Jaisson, R Garnotel, E Anglés-Can, P Gillery Small size apolipoprotein(a) isoforms enhance inflammatory and proteolytic potential of collagen-primed monocytes Lipids Health Dis, 2019;18(1):166. 2019 [PMID: 31470857] (Human) Human
B Singh, N Ghosh, D Saha, S Sarkar, P Bhattachar, K Chaudhury Effect of doxycyline in chronic obstructive pulmonary disease - An exploratory study Pulm Pharmacol Ther, 2019;0(0):101831. 2019 [PMID: 31349003] (Human) Human
B Singh, N Ghosh, D Saha, S Sarkar, P Bhattachar, K Chaudhury Effect of doxycyline in chronic obstructive pulmonary disease - An exploratory study Pulm Pharmacol Ther, 2019;0(0):101831. 2019 [PMID: 31349003] (Human) Human
L Lorente, MM Martín, L Ramos, M Argueso, JJ Cáceres, J Solé-Violá, A Jiménez, JM Borreguero, AF González-R, J Orbe, JA Rodríguez, JA Páramo High serum levels of tissue inhibitor of matrix metalloproteinase-1 during the first week of a malignant middle cerebral artery infarction in non-surviving patients BMC Neurol, 2019;19(1):167. 2019 [PMID: 31319804] (Human) Human
L Lorente, MM Martín, L Ramos, M Argueso, JJ Cáceres, J Solé-Violá, A Jiménez, JM Borreguero, AF González-R, J Orbe, JA Rodríguez, JA Páramo High serum levels of tissue inhibitor of matrix metalloproteinase-1 during the first week of a malignant middle cerebral artery infarction in non-surviving patients BMC Neurol, 2019;19(1):167. 2019 [PMID: 31319804] (Human) Human
Show All 161 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MMP-9 (DMP900). (Showing 1 - 2 of 2 FAQs).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&applications=ELISA&common_name=MMP-9It looks like 2 have been tested for capture (please note the tested species for each of these).https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Capture%20(Matched%20Antibody%20Pair)1 has been tested for detection.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Detection%20(Matched%20Antibody%20Pair)
  2. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP DMP900
HRP DMP900
HRP DMP900

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Bioinformatics

Gene Symbol MMP9