FZR1/CDH1 Antibody

Images

 
Western Blot: FZR1/CDH1 Antibody [NBP2-15840] - A431 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FZR1/CDH1 antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: FZR1/CDH1 Antibody [NBP2-15840] - Cdh1 decreases upon Abeta treatment in neurons. Representative Western blot image of cdh1 after different time points in control conditions or ...read more
Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840] - Paraffin-embedded mouse brain. FZR1 antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: FZR1/CDH1 Antibody [NBP2-15840] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FZR1 protein stained by FZR1 antibody diluted at 1:500. Red: Phalloidin, ...read more
Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840] - Paraffin-embedded mouse heart. FZR1 antibody diluted at 1:500.
Immunohistochemistry-Paraffin: FZR1/CDH1 Antibody [NBP2-15840] - Paraffin-embedded rat heart. FZR1 antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, RM, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

FZR1/CDH1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CDH1. The exact sequence is proprietary.
Localization
Isoform 2: Nucleus; Isoform 3: Cytoplasm
Predicted Species
Porcine (97%), Bovine (97%), Xenopus (90%), Rhesus Macaque (100%), Chicken (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FZR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-15840 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    3 publications

Reactivity Notes

Xenopus laevis (90%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for FZR1/CDH1 Antibody

  • CDC20C
  • CDH1
  • Cdh1/Hct1 homolog
  • CDH1CDC20-like 1b
  • fizzy/cell division cycle 20 related 1 (Drosophila)
  • Fzr
  • FZR2
  • FZRCDC20-like protein 1
  • hCDH1
  • HCDHFYR
  • KIAA1242fizzy-related protein homolog

Background

Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FZR1/CDH1 Antibody (NBP2-15840)(4)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol FZR1
Uniprot
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